Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,971,075 2 bp→GC coding (942‑943/1068 nt) rffG → dTDP‑glucose 4,6‑dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,971,0750CG100.0% 728.5 / NA 209C314W (TGC→TGG) rffGdTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (87/122);  total (87/122)
*NC_0009133,971,0760GC100.0% 732.2 / NA 209V315L (GTG→CTG) rffGdTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (87/122);  total (87/122)

Only 100 of 218 total aligned reads displayed.

TGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCGTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGAC  >  NC_000913/3970988‑3971162
                                                                                       ||                                                                                      
tGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGcc                                                                                    >  1:558541/1‑93 (MQ=255)
 gACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCg                                                                                   >  1:1670752/1‑93 (MQ=255)
    cTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAg                                                                                <  2:4431271/93‑1 (MQ=255)
     tGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAgg                                                                               >  1:5016829/1‑93 (MQ=255)
      gATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCGCGTGAACTTGGCTGGCTGCCGCAGGa                                                                              >  1:5201289/1‑93 (MQ=255)
         cACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAc                                                                           <  2:123211/93‑1 (MQ=255)
           ccTTTGTCGCTGACCGTCCGGTGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCt                                                                         <  1:137431/93‑1 (MQ=255)
           ccTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCt                                                                         <  2:2436220/93‑1 (MQ=255)
           ccTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCt                                                                         >  1:1780361/1‑93 (MQ=255)
            cTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCtt                                                                        <  1:278806/93‑1 (MQ=255)
             tttGTCGCTGCCCGTCCGGGGCATGATCTGCGTTCTGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAACttt                                                                       >  1:3155005/1‑93 (MQ=255)
              ttGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTg                                                                      <  2:5141765/93‑1 (MQ=255)
              ttGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTg                                                                      >  2:725568/1‑93 (MQ=255)
                   gCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGt                                                                 >  2:4648928/1‑93 (MQ=255)
                   gCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGt                                                                 <  2:329857/93‑1 (MQ=255)
                    cTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTg                                                                <  2:4337947/93‑1 (MQ=255)
                    cTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTg                                                                >  1:6450380/1‑93 (MQ=255)
                    cTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTg                                                                >  1:1741682/1‑93 (MQ=255)
                      gACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGa                                                              >  1:6188813/1‑93 (MQ=255)
                      gACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGa                                                              <  1:5063115/93‑1 (MQ=255)
                      gACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGa                                                              >  2:6422773/1‑93 (MQ=255)
                      gACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGa                                                              >  1:1437412/1‑93 (MQ=255)
                       aCCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGaa                                                             <  1:661477/93‑1 (MQ=255)
                       aCCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGaa                                                             <  2:3545125/93‑1 (MQ=255)
                       aCCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTGTGAAAGTGGaa                                                             >  1:4009684/1‑93 (MQ=255)
                        ccGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGaag                                                            >  1:3413606/1‑92 (MQ=255)
                        ccGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAAt                                                            <  2:4887310/93‑1 (MQ=255)
                        ccGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAAt                                                            <  2:2390048/93‑1 (MQ=255)
                         cGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATg                                                           <  2:1880838/93‑1 (MQ=255)
                          gTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGc                                                          >  2:5816053/1‑93 (MQ=255)
                          gTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGc                                                          <  1:2981656/93‑1 (MQ=255)
                            ccGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGt                                                        <  2:246746/93‑1 (MQ=255)
                             cGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTa                                                       <  1:2918804/93‑1 (MQ=255)
                             cGGGGCATGATCTGCGTTATGCCATTGATGCTACGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTa                                                       <  1:4244500/93‑1 (MQ=255)
                              ggggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTaa                                                      >  2:4089775/1‑93 (MQ=255)
                              ggggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTaa                                                      >  2:3975434/1‑93 (MQ=255)
                                ggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAACCCTTTGAAAGTGGAATGCGTaaaa                                                    <  2:3761692/93‑1 (MQ=255)
                                ggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGCAAGGGGAATGCGTaaaa                                                    >  1:6020092/1‑93 (MQ=255)
                                ggCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTaaaa                                                    >  2:2920943/1‑93 (MQ=255)
                                 gCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAAc                                                   <  2:6381271/93‑1 (MQ=255)
                                   aTGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACgg                                                 <  1:2863794/93‑1 (MQ=255)
                                    tGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGt                                                <  1:4376227/93‑1 (MQ=255)
                                     taTCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGtt                                               <  1:1275957/92‑1 (MQ=255)
                                     gATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGtt                                               >  1:5707838/1‑93 (MQ=255)
                                     gATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGtt                                               <  2:2134549/93‑1 (MQ=255)
                                     gATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGtt                                               <  2:1213556/93‑1 (MQ=255)
                                       tCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCa                                             <  2:3828627/93‑1 (MQ=255)
                                        cTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAg                                            <  1:3277847/93‑1 (MQ=255)
                                        cTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAg                                            >  2:5550884/1‑93 (MQ=255)
                                         tGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGt                                           >  1:1011618/1‑93 (MQ=255)
                                         tGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGt                                           <  1:1172145/93‑1 (MQ=255)
                                         tGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGt                                           >  1:4535435/1‑93 (MQ=255)
                                          gCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTg                                          >  1:3669431/1‑93 (MQ=255)
                                           cGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCCCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTgg                                         <  2:3447369/93‑1 (MQ=255)
                                            gTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGt                                        <  2:3099786/93‑1 (MQ=255)
                                             ttATGCCATTGAGGCTTCGAAAATGGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTa                                       <  1:5803467/93‑1 (MQ=255)
                                                tGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCt                                    <  2:2305132/93‑1 (MQ=255)
                                                tGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCt                                    <  2:6002719/93‑1 (MQ=255)
                                                tGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCt                                    >  1:1056219/1‑93 (MQ=255)
                                                 gCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTg                                   >  2:833649/1‑93 (MQ=255)
                                                 gCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTg                                   <  2:1978120/93‑1 (MQ=255)
                                                 gCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTg                                   <  1:1600583/93‑1 (MQ=255)
                                                  ccATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGTATGCGTAAAACGGTTCAGTGGTATCTgg                                  >  1:932571/1‑93 (MQ=255)
                                                  ccATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTgg                                  <  1:3804805/93‑1 (MQ=255)
                                                   cATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGGATGCGTAAAACGGTTCAGTGGTAGCTGGc                                 >  1:2020049/1‑93 (MQ=255)
                                                   cATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGc                                 <  1:2705873/93‑1 (MQ=255)
                                                    aTTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCt                                >  1:1889176/1‑93 (MQ=255)
                                                    aTTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCt                                >  2:5758325/1‑93 (MQ=255)
                                                    aTTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCt                                <  1:5300038/93‑1 (MQ=255)
                                                      tGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTaa                              <  2:1111877/93‑1 (MQ=255)
                                                      tGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTaa                              >  2:585078/1‑93 (MQ=255)
                                                        aTGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATg                            >  2:3671752/1‑93 (MQ=255)
                                                           cTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGaaa                         <  1:3860008/93‑1 (MQ=255)
                                                            ttCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAg                        <  2:2578243/93‑1 (MQ=255)
                                                                aaaaTTGCGCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtgg                    <  1:5862442/93‑1 (MQ=255)
                                                                aaaaTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtgg                    >  2:6435995/1‑93 (MQ=255)
                                                                   aTTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCtggtgg                 <  2:1513448/93‑1 (MQ=255)
                                                                    ttGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCGGTGGTATCTGGCTAATGAAAGCTGGTGGa                >  2:4380952/1‑93 (MQ=255)
                                                                    ttGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGa                <  1:4195900/93‑1 (MQ=255)
                                                                     tGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGaa               <  2:1700866/93‑1 (MQ=255)
                                                                      gCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAg              <  2:2179523/93‑1 (MQ=255)
                                                                        ccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCa            <  2:2520057/93‑1 (MQ=255)
                                                                        ccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCa            >  2:4515741/1‑93 (MQ=255)
                                                                        ccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCa            >  2:3231074/1‑93 (MQ=255)
                                                                        ccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCa            >  1:2271614/1‑93 (MQ=255)
                                                                        ccGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCa            >  1:2365754/1‑93 (MQ=255)
                                                                         cGTGAACTTGGCTGGCTGCCGCAGGAATCCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAg           <  1:1830014/93‑1 (MQ=255)
                                                                          gTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAgg          <  2:4267008/93‑1 (MQ=255)
                                                                          gTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAgg          <  2:3784356/93‑1 (MQ=255)
                                                                           tGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGt         <  1:958887/93‑1 (MQ=255)
                                                                           tGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGt         <  2:4212734/93‑1 (MQ=255)
                                                                            gAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTg        >  1:5406506/1‑93 (MQ=255)
                                                                             aaCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGc       >  1:4655985/1‑93 (MQ=255)
                                                                              aCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCa      <  2:5106433/93‑1 (MQ=255)
                                                                              aCTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCa      >  1:2147349/1‑93 (MQ=255)
                                                                                ttGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAgg    >  2:4400980/1‑93 (MQ=255)
                                                                                 tggctggcTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGa   <  1:2527441/93‑1 (MQ=255)
                                                                                 tggctggcTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGa   <  2:5401167/93‑1 (MQ=255)
                                                                                 tggctggcTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGa   <  1:771188/93‑1 (MQ=255)
                                                                                  ggctggctGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGAc  <  2:1440482/93‑1 (MQ=255)
                                                                                       ||                                                                                      
TGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCGTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGAC  >  NC_000913/3970988‑3971162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCGTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGG  >  NC_000913/3970987‑3971164
                                                                                        |                                                                                         
GTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCC                                                                                      >  SRR4302534.572309/1‑94 (MQ=60)
 TGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCG                                                                                     <  SRR4302534.5764519/94‑1 (MQ=60)
 TGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCG                                                                                     <  SRR4302534.3727645/94‑1 (MQ=60)
 TGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCG                                                                                     <  SRR4302534.1281021/94‑1 (MQ=60)
 TGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCG                                                                                     <  SRR4302534.1900513/94‑1 (MQ=60)
 TGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCG                                                                                     >  SRR4302534.1714237/1‑94 (MQ=60)
  GACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGC                                                                                    <  SRR4302534.2305264/94‑1 (MQ=60)
   ACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCA                                                                                   <  SRR4302534.3901915/94‑1 (MQ=60)
     CTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGG                                                                                 >  SRR4302534.5152386/1‑94 (MQ=60)
      TGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCGCGTGAACTTGGCTGGCTGCCGCAGGA                                                                                >  SRR4302534.5341606/1‑94 (MQ=60)
      TGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGA                                                                                >  SRR4302534.6167473/1‑94 (MQ=60)
      TGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGA                                                                                <  SRR4302534.2999800/94‑1 (MQ=60)
       GATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAA                                                                               <  SRR4302534.1670387/94‑1 (MQ=60)
           ACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCT                                                                           >  SRR4302534.1826590/1‑94 (MQ=60)
           ACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCT                                                                           >  SRR4302534.1912921/1‑94 (MQ=60)
            CCTTTGTCGCTGACCGTCCGGTGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTT                                                                          <  SRR4302534.141025/94‑1 (MQ=60)
             CTTTGTCGCTGCCCGTCCGGGGCATGATCTGCGTTCTGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAAACTTT                                                                         >  SRR4302534.3238096/1‑94 (MQ=60)
             CTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTT                                                                         >  SRR4302534.5296666/1‑94 (MQ=60)
             CTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTT                                                                         <  SRR4302534.286022/94‑1 (MQ=60)
               TTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGA                                                                       <  SRR4302534.547494/94‑1 (MQ=60)
                TGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAA                                                                      >  SRR4302534.5920963/1‑94 (MQ=60)
                  TCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAG                                                                    <  SRR4302534.1268867/94‑1 (MQ=60)
                  TCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAG                                                                    <  SRR4302534.3671339/94‑1 (MQ=60)
                    GCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTG                                                                  >  SRR4302534.1786898/1‑94 (MQ=60)
                    GCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTG                                                                  <  SRR4302534.2975068/94‑1 (MQ=60)
                    GCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTG                                                                  >  SRR4302534.6624210/1‑94 (MQ=60)
                      TGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGA                                                                >  SRR4302534.6357237/1‑94 (MQ=60)
                      TGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGA                                                                >  SRR4302534.1455701/1‑94 (MQ=60)
                      TGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGA                                                                >  SRR4302534.1475248/1‑94 (MQ=60)
                       GACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAA                                                               <  SRR4302534.5199832/94‑1 (MQ=60)
                       GACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAA                                                               >  SRR4302534.2750429/1‑94 (MQ=60)
                       GACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTGTGAAAGTGGAA                                                               >  SRR4302534.4117055/1‑94 (MQ=60)
                        ACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAAT                                                              <  SRR4302534.677564/94‑1 (MQ=60)
                        ACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAAG                                                              >  SRR4302534.3505301/1‑94 (MQ=60)
                         CCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATG                                                             <  SRR4302534.1830983/94‑1 (MQ=60)
                           GTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCG                                                           <  SRR4302534.3059676/94‑1 (MQ=60)
                           GTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCG                                                           >  SRR4302534.6039251/1‑94 (MQ=60)
                              CGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAA                                                        <  SRR4302534.675855/94‑1 (MQ=60)
                              CGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAA                                                        <  SRR4302534.2995397/94‑1 (MQ=60)
                              CGGGGCATGATCTGCGTTATGCCATTGATGCTACGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAA                                                        <  SRR4302534.4358976/94‑1 (MQ=60)
                               gtcGCATGATCTGCGCTATGGCGTTGATTCTGCGAAAATTGCCCGTGAACTTGGCTGGCTGACGCAGGAAACCTTTGAAAGTGGAATGCGTAAA                                                       <  SRR4302534.3228271/91‑1 (MQ=59)
                               GGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAA                                                       >  SRR4302534.5379678/1‑94 (MQ=60)
                               GGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAA                                                       >  SRR4302534.4322581/1‑94 (MQ=60)
                                CGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGCAAGGGGAATGCGTAAAA                                                      >  SRR4302534.6183692/1‑94 (MQ=60)
                                 GGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGAAAAAC                                                     <  SRR4302534.6255363/94‑1 (MQ=60)
                                  GCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACG                                                    >  SRR4302534.105824/1‑94 (MQ=60)
                                   CATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGG                                                   >  SRR4302534.6568092/1‑94 (MQ=60)
                                   CATGATCTGCGTTATGCCATCGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGG                                                   >  SRR4302534.6407038/1‑94 (MQ=60)
                                    ATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGT                                                  <  SRR4302534.2939011/94‑1 (MQ=60)
                                     TGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTT                                                 >  SRR4302534.5862465/1‑94 (MQ=60)
                                     TGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTT                                                 <  SRR4302534.4494182/94‑1 (MQ=60)
                                      TATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTC                                                <  SRR4302534.1309538/94‑1 (MQ=60)
                                      GATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTC                                                <  SRR4302534.2382614/94‑1 (MQ=60)
                                         CTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGT                                             <  SRR4302534.4058331/94‑1 (MQ=60)
                                         CTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGT                                             >  SRR4302534.4225883/1‑94 (MQ=60)
                                         CTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGT                                             <  SRR4302534.4359610/94‑1 (MQ=60)
                                         CTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGT                                             >  SRR4302534.4658345/1‑94 (MQ=60)
                                         CTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGT                                             <  SRR4302534.3365201/94‑1 (MQ=60)
                                         CTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGT                                             >  SRR4302534.3348074/1‑94 (MQ=60)
                                         CTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGT                                             >  SRR4302534.1156691/1‑94 (MQ=60)
                                         CTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGT                                             >  SRR4302534.1037639/1‑94 (MQ=60)
                                          TGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTG                                            <  SRR4302534.1202958/94‑1 (MQ=60)
                                          TGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTG                                            >  SRR4302534.3768078/1‑94 (MQ=60)
                                          TGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTG                                            <  SRR4302534.5671503/94‑1 (MQ=60)
                                           GCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGGTCAGTGG                                           >  SRR4302534.4578589/1‑94 (MQ=60)
                                            CGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGT                                          >  SRR4302534.2045344/1‑94 (MQ=60)
                                              TTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTAT                                        <  SRR4302534.5637857/94‑1 (MQ=60)
                                              TTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTAT                                        <  SRR4302534.4862061/94‑1 (MQ=60)
                                              TTATGCCATTGAGGCTTCGAAAATGGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTAT                                        <  SRR4302534.5960966/94‑1 (MQ=60)
                                               TATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATC                                       <  SRR4302534.340235/94‑1 (MQ=60)
                                                ATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCT                                      >  SRR4302534.1083563/1‑94 (MQ=60)
                                                 TGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTG                                     <  SRR4302534.1493169/94‑1 (MQ=60)
                                                  GCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGTATGCGTAAAACGGTTCAGTGGTATCTGG                                    >  SRR4302534.956296/1‑94 (MQ=60)
                                                  GCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGG                                    <  SRR4302534.1642399/94‑1 (MQ=60)
                                                   CCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGGATGCGTAAAACGGTTCAGTGGTAGCTGGC                                   >  SRR4302534.2072904/1‑94 (MQ=60)
                                                   CCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGC                                   <  SRR4302534.3906967/94‑1 (MQ=60)
                                                    CATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCT                                  >  SRR4302534.1938440/1‑94 (MQ=60)
                                                    CATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCT                                  <  SRR4302534.2777328/94‑1 (MQ=60)
                                                     ATTGATTCTTCGAAAATTGCCCGTGAACTTGGCTGTCTGCCGCAGGAAACCTATGAAAGTGCAATGCGTAAAGCGGTACAGTGGTATCTGGCTA                                 <  SRR4302534.5982533/94‑1 (MQ=60)
                                                     ATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTA                                 <  SRR4302534.5443059/94‑1 (MQ=60)
                                                       TGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAAT                               >  SRR4302534.5012367/1‑94 (MQ=60)
                                                       TGATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAAT                               <  SRR4302534.1016676/94‑1 (MQ=60)
                                                         ATGCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGA                             <  SRR4302534.3345365/94‑1 (MQ=60)
                                                           GCTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAA                           <  SRR4302534.1820699/94‑1 (MQ=60)
                                                            CTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAG                          <  SRR4302534.3963646/94‑1 (MQ=60)
                                                            CTTCGAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAG                          <  SRR4302534.3942615/94‑1 (MQ=60)
                                                                GAAAATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGG                      >  SRR4302534.2107536/1‑94 (MQ=60)
                                                                 AAAATTGCGCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGT                     <  SRR4302534.6021609/94‑1 (MQ=60)
                                                                 AAAATTGCCCGTGTACTTGGCTGGCTGCCGCAGGAAACCTCTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGGTAATGAAAGCggtt                     >  SRR4302534.4889981/1‑90 (MQ=60)
                                                                   AATTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGG                   <  SRR4302534.2822680/94‑1 (MQ=60)
                                                                     TTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAA                 <  SRR4302534.4309244/94‑1 (MQ=60)
                                                                     TTGCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAA                 <  SRR4302534.5194666/94‑1 (MQ=60)
                                                                       GCCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGC               <  SRR4302534.174487/94‑1 (MQ=60)
                                                                        CCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTGAAACGGGTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCA              >  SRR4302534.979282/1‑94 (MQ=60)
                                                                        CCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCA              >  SRR4302534.2331849/1‑94 (MQ=60)
                                                                        CCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCA              >  SRR4302534.5810821/1‑94 (MQ=60)
                                                                        CCCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCA              >  SRR4302534.2428564/1‑94 (MQ=60)
                                                                         CCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAG             <  SRR4302534.1378248/94‑1 (MQ=60)
                                                                         CCGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAG             <  SRR4302534.4079545/94‑1 (MQ=60)
                                                                          CGTGAACTTGGCTGGCTGCCGCAGGAATCCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGG            <  SRR4302534.1877612/94‑1 (MQ=60)
                                                                          CGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGG            >  SRR4302534.767786/1‑94 (MQ=60)
                                                                          CGTGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGG            <  SRR4302534.2903314/94‑1 (MQ=60)
                                                                            TGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTG          <  SRR4302534.983316/94‑1 (MQ=60)
                                                                            TGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTG          >  SRR4302534.5551982/1‑94 (MQ=60)
                                                                            TGAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTG          <  SRR4302534.4696881/94‑1 (MQ=60)
                                                                             GAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGC         >  SRR4302534.4782384/1‑94 (MQ=60)
                                                                             GAACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGC         <  SRR4302534.856413/94‑1 (MQ=60)
                                                                              AACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCA        <  SRR4302534.2616365/94‑1 (MQ=60)
                                                                              AACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCA        >  SRR4302534.2204018/1‑94 (MQ=60)
                                                                               ACTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAG       <  SRR4302534.1612688/94‑1 (MQ=60)
                                                                                CTTGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGG      >  SRR4302534.2487073/1‑94 (MQ=60)
                                                                                  TGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGAC    <  SRR4302534.2594511/94‑1 (MQ=60)
                                                                                  TGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGAC    <  SRR4302534.790377/94‑1 (MQ=60)
                                                                                  TGGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGAC    <  SRR4302534.101782/94‑1 (MQ=60)
                                                                                   GGCTGGCTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACG   <  SRR4302534.2055091/94‑1 (MQ=60)
                                                                                    GCTGGCTGCCGCAGGAATCGTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGCCTAATGAAAGCTGGTGTAAGCAGGTGCAGGACGG  <  SRR4302534.4789910/94‑1 (MQ=60)
                                                                                        |                                                                                         
GTGACCTGATCACCTTTGTCGCTGACCGTCCGGGGCATGATCTGCGTTATGCCATTGATGCTTCGAAAATTGCCCGTGAACTTGGCTGCGTGCCGCAGGAAACCTTTGAAAGTGGAATGCGTAAAACGGTTCAGTGGTATCTGGCTAATGAAAGCTGGTGGAAGCAGGTGCAGGACGG  >  NC_000913/3970987‑3971164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑