Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,004,984 +CG coding (1516/1833 nt) recQ → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,004,9831.G99.1% 769.2 / ‑1.0 226coding (1515/1833 nt)recQATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (126/97);  total (126/100)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01
*NC_0009134,004,9832.C98.7% 771.9 / 1.8 226coding (1515/1833 nt)recQATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base . (0/3);  new base C (126/97);  total (126/100)
Fisher's exact test for biased strand distribution p-value = 8.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 242 total aligned reads displayed.

CCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA  >  NC_000913/4004893‑4005060
                                                                                            ||                                                                               
ccGCCAGCTGATTCACCTCGGCCTTGCGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTCCTGCGCg‑‑g                                                                                >  1:5322054/1‑93 (MQ=255)
ccGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑g                                                                                <  2:4085213/93‑1 (MQ=255)
 cGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑gc                                                                               <  1:5825626/93‑2 (MQ=255)
  gCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGACGCGCGCCCGGTGCTGCGCg‑‑gcg                                                                              >  1:2076544/1‑91 (MQ=255)
   ccAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑gcga                                                                             >  2:2635384/1‑90 (MQ=255)
   ccAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑gcga                                                                             >  2:2818053/1‑90 (MQ=255)
    cAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑gcgaa                                                                            >  1:1120973/1‑89 (MQ=255)
          aTTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCctct                                                                      >  2:2990603/1‑93 (MQ=255)
           ttCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCtt                                                                     <  2:5799061/93‑1 (MQ=255)
            tCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCttt                                                                    >  1:3408637/1‑93 (MQ=255)
             cACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGACGTCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTg                                                                   >  1:1525301/1‑93 (MQ=255)
               ccTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCa                                                                 >  1:76520/1‑93 (MQ=255)
                  gggCCTGGTGACGCAAAATATTGCGCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACt                                                              <  1:3477166/92‑1 (MQ=255)
                   ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACtt                                                             <  1:1503351/93‑1 (MQ=255)
                   ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACtt                                                             <  2:3224096/93‑1 (MQ=255)
                   ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACtt                                                             <  2:2520177/93‑1 (MQ=255)
                      cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgcc                                                          >  2:2007408/1‑93 (MQ=255)
                      cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgcc                                                          >  2:153339/1‑93 (MQ=255)
                       tGGTGACGCAAAATATTGCCCATCATTCTGCCCTACAACAGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccg                                                         <  1:4169618/93‑1 (MQ=255)
                       tGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccg                                                         >  2:193799/1‑93 (MQ=255)
                       tGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccg                                                         <  2:588515/93‑1 (MQ=255)
                        ggTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgt                                                        >  1:1794270/1‑93 (MQ=255)
                        ggTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgt                                                        >  1:2201952/1‑93 (MQ=255)
                        ggTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgt                                                        <  2:4210885/93‑1 (MQ=255)
                        ggTGACGCAAAATATGGCCCATCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgt                                                        <  1:3157119/93‑1 (MQ=255)
                          tGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtgc                                                      <  1:6094738/93‑1 (MQ=255)
                          tGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtgc                                                      <  2:3050715/93‑1 (MQ=255)
                           gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtgcc                                                     >  2:4675464/1‑93 (MQ=255)
                            aCGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtgccg                                                    >  1:4502750/1‑93 (MQ=255)
                             cGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCcgc                                                   <  1:954626/93‑1 (MQ=255)
                             cGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCcgc                                                   <  2:5517915/93‑1 (MQ=255)
                             cGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCcgc                                                   <  1:4545938/93‑1 (MQ=255)
                              gCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCcgcg                                                  <  1:4057141/93‑1 (MQ=255)
                                 aaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTAt                                               <  2:2303974/93‑1 (MQ=255)
                                  aaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATc                                              >  2:4036507/1‑93 (MQ=255)
                                   atatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCg                                             >  2:3338050/1‑93 (MQ=255)
                                    tatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGt                                            <  1:1706283/93‑1 (MQ=255)
                                     atTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTg                                           <  1:5661739/93‑1 (MQ=255)
                                      ttGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTgg                                          <  2:4849035/93‑1 (MQ=255)
                                      ttGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTgg                                          >  2:1453881/1‑93 (MQ=255)
                                       tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgc                                         <  1:4126561/93‑1 (MQ=255)
                                       tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgc                                         <  1:3727594/93‑1 (MQ=255)
                                       tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgc                                         <  2:5154474/93‑1 (MQ=255)
                                        gCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgcg                                        >  1:5097392/1‑93 (MQ=255)
                                          ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                      >  1:4308571/1‑93 (MQ=255)
                                          ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                      >  1:3893390/1‑93 (MQ=255)
                                          ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                      >  1:3112196/1‑93 (MQ=255)
                                          ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                      >  1:2583600/1‑93 (MQ=255)
                                           cAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTc                                     >  2:4390949/1‑93 (MQ=255)
                                           cAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTc                                     >  1:1013467/1‑93 (MQ=255)
                                            cgCATTCTCCCCCAGAACTGACAGAGGCCGCGCCCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                    <  2:1612107/92‑1 (MQ=255)
                                            aGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                    >  1:685176/1‑93 (MQ=255)
                                            aGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                    <  1:1246157/93‑1 (MQ=255)
                                            aGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                    >  2:6018738/1‑93 (MQ=255)
                                             gCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaa                                   <  2:5545321/93‑1 (MQ=255)
                                               aTTCTGCCCTACAACTGACAGAGGGCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                 >  1:6226757/1‑93 (MQ=255)
                                               aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                 <  1:5592055/93‑1 (MQ=255)
                                                ttCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAcc                                >  2:2156682/1‑93 (MQ=255)
                                                ttCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAcc                                <  1:545846/93‑1 (MQ=255)
                                                 tCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCg                               <  2:775790/93‑1 (MQ=255)
                                                  cTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGa                              >  2:3823796/1‑93 (MQ=255)
                                                   tGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaa                             >  2:3259203/1‑93 (MQ=255)
                                                    gCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCG‑‑GCTAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaaa                            <  1:1913622/93‑1 (MQ=255)
                                                     cccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg                           <  2:640998/93‑1 (MQ=255)
                                                      ccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          <  2:2905697/93‑1 (MQ=255)
                                                      ccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          <  2:6187260/93‑1 (MQ=255)
                                                       cTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCg                         <  2:5475407/93‑1 (MQ=255)
                                                        tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                        <  2:3732020/93‑1 (MQ=255)
                                                        tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                        <  1:2300997/93‑1 (MQ=255)
                                                         aCAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAt                       >  1:5105000/1‑93 (MQ=255)
                                                           aaCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                     <  1:3588192/93‑1 (MQ=255)
                                                            aCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    >  2:2049498/1‑93 (MQ=255)
                                                            aCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    >  2:6428107/1‑93 (MQ=255)
                                                            aCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    >  2:4486804/1‑93 (MQ=255)
                                                               gACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaa                 >  2:501401/1‑93 (MQ=255)
                                                                aCAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaaa                <  2:3377268/93‑1 (MQ=255)
                                                                aCAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaaa                >  2:860837/1‑93 (MQ=255)
                                                                 cAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt               <  2:3524281/93‑1 (MQ=255)
                                                                 cAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt               <  2:4994591/93‑1 (MQ=255)
                                                                  agagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATc              <  1:495865/93‑1 (MQ=255)
                                                                   gagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg             <  2:5714048/93‑1 (MQ=255)
                                                                   gagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg             >  2:1637728/1‑93 (MQ=255)
                                                                   gagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAGCg             >  1:2272424/1‑93 (MQ=255)
                                                                    agGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGt            >  2:1703361/1‑93 (MQ=255)
                                                                    agGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGt            <  1:2679057/93‑1 (MQ=255)
                                                                     ggCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           >  2:4914797/1‑93 (MQ=255)
                                                                     ggCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           <  1:5178181/93‑1 (MQ=255)
                                                                     ggCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           <  2:1285284/93‑1 (MQ=255)
                                                                     ggCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           >  2:1860798/1‑93 (MQ=255)
                                                                      gCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTc          <  2:1054313/93‑1 (MQ=255)
                                                                       ccGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcg         <  1:2982148/93‑1 (MQ=255)
                                                                        cgcgcgCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcgg        >  1:536174/1‑93 (MQ=255)
                                                                         gcgcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       >  2:5294461/1‑93 (MQ=255)
                                                                         gcgcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       >  1:5876755/1‑93 (MQ=255)
                                                                          cgcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcg      <  2:6055417/93‑1 (MQ=255)
                                                                           gcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcgg     <  2:3060163/93‑1 (MQ=255)
                                                                             gcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCa   >  1:4668474/1‑93 (MQ=255)
                                                                             gcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCa   >  2:5970097/1‑93 (MQ=255)
                                                                              cccGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCaa  >  1:4400735/1‑93 (MQ=255)
                                                                              cccGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGCGGCGCTCAAACCGCAAGCGATGCAGAAATCGTTCGGCGGGaa  >  2:2751905/1‑93 (MQ=255)
                                                                                            ||                                                                               
CCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA  >  NC_000913/4004893‑4005060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGT  >  NC_000913/4004892‑4005077
                                                                                               |                                                                                               
TCCGCCAGCTGATTCACCTCGGCCTTGCGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTCCTGCGCG‑‑G                                                                                                 >  SRR4302534.5465594/1‑94 (MQ=60)
        CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCT                                                                                         >  SRR4302534.2673694/1‑94 (MQ=60)
        CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCT                                                                                         <  SRR4302534.1210310/94‑1 (MQ=60)
        CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCT                                                                                         >  SRR4302534.3344603/1‑94 (MQ=60)
        CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCT                                                                                         <  SRR4302534.3506542/94‑1 (MQ=60)
        CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCT                                                                                         <  SRR4302534.4330616/94‑1 (MQ=60)
          GATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGCCAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCT                                                                                       <  SRR4302534.1844409/94‑1 (MQ=60)
          GATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCT                                                                                       >  SRR4302534.1911995/1‑94 (MQ=60)
           ATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTT                                                                                      >  SRR4302534.1254167/1‑94 (MQ=60)
            TTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTT                                                                                     >  SRR4302534.3500190/1‑94 (MQ=60)
            TTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTT                                                                                     >  SRR4302534.2710862/1‑94 (MQ=60)
             TCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTG                                                                                    >  SRR4302534.1374656/1‑94 (MQ=60)
             TCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGACGTCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTG                                                                                    >  SRR4302534.1565347/1‑94 (MQ=60)
              CACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGC                                                                                   <  SRR4302534.2117535/94‑1 (MQ=60)
               ACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCA                                                                                  >  SRR4302534.78598/1‑94 (MQ=60)
                CCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAA                                                                                 <  SRR4302534.3373630/94‑1 (MQ=60)
                 CTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAAC                                                                                >  SRR4302534.621398/1‑94 (MQ=60)
                 CTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAAC                                                                                >  SRR4302534.3702674/1‑94 (MQ=60)
                   GGGCCTGGTGACGCAAAATATTGCGCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTT                                                                              <  SRR4302534.3570628/94‑1 (MQ=60)
                   CGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTT                                                                              >  SRR4302534.4179522/1‑94 (MQ=60)
                   CGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTT                                                                              >  SRR4302534.5804001/1‑94 (MQ=60)
                   CGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTT                                                                              >  SRR4302534.1392796/1‑94 (MQ=60)
                    GGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTG                                                                             <  SRR4302534.1542906/94‑1 (MQ=60)
                      CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCC                                                                           >  SRR4302534.2263381/1‑94 (MQ=60)
                      CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCC                                                                           >  SRR4302534.523158/1‑94 (MQ=60)
                       CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCG                                                                          <  SRR4302534.5848413/94‑1 (MQ=60)
                        TGGTGACGCAAAATATTGCCCATCATTCTGCCCTACAACAGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGT                                                                         <  SRR4302534.4282249/94‑1 (MQ=60)
                        TGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGT                                                                         >  SRR4302534.1840872/1‑94 (MQ=60)
                        TGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGT                                                                         >  SRR4302534.2260210/1‑94 (MQ=60)
                        TGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGT                                                                         <  SRR4302534.6283004/94‑1 (MQ=60)
                         GGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTG                                                                        <  SRR4302534.3625645/94‑1 (MQ=60)
                         GGTGACGCAAAATATGGCCCATCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTG                                                                        <  SRR4302534.3240273/94‑1 (MQ=60)
                          GTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGC                                                                       <  SRR4302534.2254923/94‑1 (MQ=60)
                           TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCC                                                                      <  SRR4302534.5865769/94‑1 (MQ=60)
                           TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCC                                                                      <  SRR4302534.6260479/94‑1 (MQ=60)
                           TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCC                                                                      <  SRR4302534.3181587/94‑1 (MQ=60)
                            GACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCG                                                                     >  SRR4302534.4624621/1‑94 (MQ=60)
                            GACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCG                                                                     <  SRR4302534.1127131/94‑1 (MQ=60)
                             ACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGC                                                                    >  SRR4302534.2271185/1‑94 (MQ=60)
                             ACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGC                                                                    >  SRR4302534.751358/1‑94 (MQ=60)
                              CGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCG                                                                   >  SRR4302534.3542391/1‑94 (MQ=60)
                              CGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCG                                                                   <  SRR4302534.457280/94‑1 (MQ=60)
                              CGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCG                                                                   <  SRR4302534.978933/94‑1 (MQ=60)
                              CGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCG                                                                   <  SRR4302534.4669184/94‑1 (MQ=60)
                               GCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGT                                                                  <  SRR4302534.4165967/94‑1 (MQ=60)
                                CAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTA                                                                 <  SRR4302534.3938597/94‑1 (MQ=60)
                                   AATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCG                                                              <  SRR4302534.5626333/94‑1 (MQ=60)
                                     TATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTG                                                            <  SRR4302534.1750612/94‑1 (MQ=60)
                                      ATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGG                                                           <  SRR4302534.2499838/94‑1 (MQ=60)
                                      ATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGG                                                           <  SRR4302534.5815026/94‑1 (MQ=60)
                                        TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                                         <  SRR4302534.3827775/94‑1 (MQ=60)
                                        TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                                         >  SRR4302534.5512156/1‑94 (MQ=60)
                                        TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                                         >  SRR4302534.5234969/1‑94 (MQ=60)
                                        TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                                         >  SRR4302534.1588860/1‑94 (MQ=60)
                                        TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                                         <  SRR4302534.4237670/94‑1 (MQ=60)
                                          CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                                       >  SRR4302534.3997892/1‑94 (MQ=60)
                                          CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                                       <  SRR4302534.4030523/94‑1 (MQ=60)
                                          CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                                       >  SRR4302534.3194038/1‑94 (MQ=60)
                                          CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                                       >  SRR4302534.2651996/1‑94 (MQ=60)
                                          CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                                       >  SRR4302534.4425324/1‑94 (MQ=60)
                                           CCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTC                                                      >  SRR4302534.1039547/1‑94 (MQ=60)
                                            CAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCA                                                     >  SRR4302534.701912/1‑94 (MQ=60)
                                             AGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAA                                                    >  SRR4302534.6103205/1‑94 (MQ=60)
                                             AGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAA                                                    <  SRR4302534.1279001/94‑1 (MQ=60)
                                              GCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAA                                                   >  SRR4302534.978000/1‑94 (MQ=60)
                                              GCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAA                                                   <  SRR4302534.4268062/94‑1 (MQ=60)
                                               CATTCTGCCCTACAACTGACAGAGGGCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                                  >  SRR4302534.6395810/1‑94 (MQ=60)
                                               CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                                  <  SRR4302534.6640702/94‑1 (MQ=60)
                                                ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                                 <  SRR4302534.5743235/94‑1 (MQ=60)
                                                 TTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCG                                                <  SRR4302534.559327/94‑1 (MQ=60)
                                                 TTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCG                                                <  SRR4302534.2084036/94‑1 (MQ=60)
                                                  TCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGA                                               <  SRR4302534.5501901/94‑1 (MQ=60)
                                                   CTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAA                                              >  SRR4302534.4768195/1‑94 (MQ=60)
                                                   CTGCCCTACAACTGACAGAGGCAGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAA                                              >  SRR4302534.5266767/1‑94 (MQ=60)
                                                     GCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAG                                            <  SRR4302534.5121173/94‑1 (MQ=60)
                                                     GCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCG‑‑GCTAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAG                                            <  SRR4302534.1963570/94‑1 (MQ=60)
                                                      CCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                                           >  SRR4302534.3821755/1‑94 (MQ=60)
                                                      CCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                                           <  SRR4302534.4259596/94‑1 (MQ=60)
                                                       CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                                          >  SRR4302534.2360798/1‑94 (MQ=60)
                                                         TACAGCTGACAGAGGCCGCGCGCCCGGTGCTGCGCG‑‑GCAAATCCTCTTTGCAGCTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                                        <  SRR4302534.3098910/94‑1 (MQ=60)
                                                         TACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                                        <  SRR4302534.2362069/94‑1 (MQ=60)
                                                         TACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                                        >  SRR4302534.5242766/1‑94 (MQ=60)
                                                          TCAACTGACAAAGGCCTCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATG                                       <  SRR4302534.341198/94‑1 (MQ=60)
                                                          ACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATG                                       <  SRR4302534.4406359/94‑1 (MQ=60)
                                                          ACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATG                                       <  SRR4302534.5584220/94‑1 (MQ=60)
                                                          ACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATG                                       <  SRR4302534.5337737/94‑1 (MQ=60)
                                                            AACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                                     >  SRR4302534.1543915/1‑94 (MQ=60)
                                                            AACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                                     <  SRR4302534.3684699/94‑1 (MQ=60)
                                                             ACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAG                                    <  SRR4302534.4583889/94‑1 (MQ=60)
                                                                GACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAA                                 >  SRR4302534.299646/1‑94 (MQ=60)
                                                                 ACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAT                                >  SRR4302534.5149741/1‑94 (MQ=60)
                                                                  CAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                               >  SRR4302534.4113195/1‑94 (MQ=60)
                                                                  CAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                               <  SRR4302534.4885373/94‑1 (MQ=60)
                                                                   AGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCG                              >  SRR4302534.1586253/1‑94 (MQ=60)
                                                                   AGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCG                              <  SRR4302534.508239/94‑1 (MQ=60)
                                                                   AGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAGCG                              >  SRR4302534.2332684/1‑94 (MQ=60)
                                                                   AGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGAGATGCAGAAATCG                              >  SRR4302534.2475015/1‑94 (MQ=60)
                                                                    GAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGT                             >  SRR4302534.6506839/1‑94 (MQ=60)
                                                                    GAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGT                             <  SRR4302534.4428809/94‑1 (MQ=60)
                                                                     AGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTT                            <  SRR4302534.2749878/94‑1 (MQ=60)
                                                                      GGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTC                           <  SRR4302534.5317698/94‑1 (MQ=60)
                                                                        CCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGG                         <  SRR4302534.3060177/94‑1 (MQ=60)
                                                                        CCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGG                         >  SRR4302534.4772285/1‑94 (MQ=60)
                                                                        CCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGG                         >  SRR4302534.549472/1‑94 (MQ=60)
                                                                         CGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGC                        >  SRR4302534.1774212/1‑94 (MQ=60)
                                                                         CGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGC                        >  SRR4302534.6036308/1‑94 (MQ=60)
                                                                         CGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGC                        >  SRR4302534.6009128/1‑94 (MQ=60)
                                                                          GCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCG                       >  SRR4302534.3407002/1‑94 (MQ=60)
                                                                             CGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGTTGCAGAAATCGTTCGGCGGCA                    <  SRR4302534.5393899/94‑1 (MQ=60)
                                                                             CGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCA                    >  SRR4302534.4646921/1‑94 (MQ=60)
                                                                             CGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCA                    >  SRR4302534.4795210/1‑94 (MQ=60)
                                                                              GCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA                   >  SRR4302534.4519470/1‑94 (MQ=60)
                                                                                CCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT                 >  SRR4302534.621843/1‑94 (MQ=60)
                                                                                 CGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA                <  SRR4302534.4631828/94‑1 (MQ=60)
                                                                                 CGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA                <  SRR4302534.2879173/94‑1 (MQ=60)
                                                                                    tgctgcgcggcG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGA             <  SRR4302534.2349128/83‑1 (MQ=60)
                                                                                    tgctgcgcggcG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGA             <  SRR4302534.3415909/83‑1 (MQ=60)
                                                                                      ctgcgcggcG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATC           <  SRR4302534.3595421/85‑1 (MQ=60)
                                                                                      ctgcgcggcG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATC           <  SRR4302534.6015216/85‑1 (MQ=60)
                                                                                       ttcgcggcG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCG          <  SRR4302534.1573819/86‑1 (MQ=60)
                                                                                       tgcgcggcG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCG          <  SRR4302534.3494116/86‑1 (MQ=60)
                                                                                       tgcgcggcG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCG          >  SRR4302534.269546/9‑94 (MQ=60)
                                                                                        gcggggcG‑‑AATCCTCTTTGCACCTCGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGCCGGCAACTATGATCGC         <  SRR4302534.1439024/87‑1 (MQ=60)
                                                                                        gcgcggcG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGC         <  SRR4302534.5616308/87‑1 (MQ=60)
                                                                                        gcgcggcG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGC         <  SRR4302534.3323181/87‑1 (MQ=60)
                                                                                         CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCcga        >  SRR4302534.5194779/1‑91 (MQ=60)
                                                                                         CGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCA        >  SRR4302534.3515844/1‑94 (MQ=60)
                                                                                            ggcG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAAC     >  SRR4302534.3210784/4‑94 (MQ=60)
                                                                                            C‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTG   <  SRR4302534.149240/94‑1 (MQ=60)
                                                                                            C‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTG   <  SRR4302534.443965/94‑1 (MQ=60)
                                                                                             gcG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACT    <  SRR4302534.2892179/92‑1 (MQ=60)
                                                                                             gcG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGAGCCAACTATGATCGAAAACG    >  SRR4302534.4365348/3‑94 (MQ=60)
                                                                                               G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGT  >  SRR4302534.5593532/1‑94 (MQ=60)
                                                                                               G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGT  >  SRR4302534.2227604/1‑94 (MQ=60)
                                                                                               G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGT  <  SRR4302534.1339304/94‑1 (MQ=60)
                                                                                               |                                                                                               
TCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTATGATCGCAAACTGT  >  NC_000913/4004892‑4005077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑