Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,016,796	T→C	Y119H (TAT→CAT)	ubiE →	UbiE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,016,796	0	T	C	99.6%	954.9 / NA	272	Y119H (TAT→CAT)	ubiE	UbiE
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (144/127); total (145/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 272 total aligned reads displayed.

GGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGAC  >  NC_000913/4016706‑4016885

  cAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGt                                                                                       <  1:3840154/93‑1 (MQ=255)

  cAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGt                                                                                       >  2:4539247/1‑93 (MQ=255)

  cAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGt                                                                                       >  2:4364089/1‑93 (MQ=255)

     aGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCa                                                                                    >  2:703867/1‑93 (MQ=255)

        ggTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGc                                                                                 >  1:639001/1‑93 (MQ=255)

        ggTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGc                                                                                 >  1:3257097/1‑93 (MQ=255)

        ggTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGc                                                                                 >  2:5474036/1‑93 (MQ=255)

        ggTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGc                                                                                 <  2:1358689/93‑1 (MQ=255)

        ggTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGc                                                                                 <  1:4968826/93‑1 (MQ=255)

         gTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCg                                                                                <  2:971991/93‑1 (MQ=255)

         gTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCg                                                                                <  1:5464069/93‑1 (MQ=255)

          tCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGa                                                                               >  1:1508271/1‑93 (MQ=255)

          tCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGa                                                                               <  2:4643100/93‑1 (MQ=255)

           ccTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGaa                                                                              >  1:3706357/1‑93 (MQ=255)

            cTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAAc                                                                             <  1:5771030/93‑1 (MQ=255)

                cTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTg                                                                         >  2:4443694/1‑93 (MQ=255)

                  gATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCCTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAg                                                                       >  1:533045/1‑93 (MQ=255)

                   atatCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAgg                                                                      <  1:514710/93‑1 (MQ=255)

                   atatCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGGTCAGGCGAACGCTGAgg                                                                      >  1:1836405/1‑93 (MQ=255)

                    tatCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGgc                                                                     <  1:2887846/93‑1 (MQ=255)

                    tatCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGgc                                                                     >  2:5894621/1‑93 (MQ=255)

                    tatCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGgc                                                                     <  2:1146915/93‑1 (MQ=255)

                      tCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGgcgc                                                                   >  1:5082923/1‑93 (MQ=255)

                      tCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGgcgc                                                                   >  1:1000375/1‑93 (MQ=255)

                      tCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGgcgc                                                                   <  1:2038499/93‑1 (MQ=255)

                        aaTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTg                                                                 <  2:3181453/93‑1 (MQ=255)

                        aaTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTg                                                                 >  2:3136805/1‑93 (MQ=255)

                         aTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCATGCGAACGCTGAGGCGCTGc                                                                <  1:6239046/93‑1 (MQ=255)

                         aTGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGc                                                                <  2:4123721/93‑1 (MQ=255)

                          tGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGcc                                                               >  2:2078959/1‑93 (MQ=255)

                          tGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGcc                                                               <  1:2974432/93‑1 (MQ=255)

                            aaTCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAGGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGt                                                             <  1:6150960/93‑1 (MQ=255)

                            aaTCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGt                                                             >  1:5693558/1‑93 (MQ=255)

                             aTCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGtt                                                            >  2:2879064/1‑93 (MQ=255)

                              tCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTc                                                           >  1:3141720/1‑93 (MQ=255)

                                cATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTccc                                                         >  1:2510468/1‑93 (MQ=255)

                                cATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTccc                                                         >  1:1677721/1‑93 (MQ=255)

                                cATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTccc                                                         <  1:2735496/93‑1 (MQ=255)

                                cATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTccc                                                         <  2:2701396/93‑1 (MQ=255)

                                 aTGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCg                                                        >  2:1278601/1‑93 (MQ=255)

                                   gctcAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGa                                                      <  1:1262251/90‑1 (MQ=255)

                                   gctcAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGa                                                      <  1:81949/90‑1 (MQ=255)

                                    ctcAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGAt                                                     <  2:420662/91‑1 (MQ=255)

                                     tcAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATa                                                    <  1:2355189/92‑1 (MQ=255)

                                     tcAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATa                                                    <  2:4163732/92‑1 (MQ=255)

                                      cAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATaa                                                   <  1:785695/93‑1 (MQ=255)

                                        aaaTGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAaca                                                 >  2:6002940/1‑93 (MQ=255)

                                        aaaTGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAaca                                                 >  1:1718459/1‑93 (MQ=255)

                                         aaTGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAacac                                                >  1:4289670/1‑93 (MQ=255)

                                          aTGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACAcc                                               >  2:5830413/1‑93 (MQ=255)

                                          aTGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACAcc                                               >  2:3565427/1‑93 (MQ=255)

                                           tGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCt                                              >  2:4827994/1‑93 (MQ=255)

                                            gggCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCtt                                             <  2:5182152/93‑1 (MQ=255)

                                            gggCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCtt                                             <  1:4799415/93‑1 (MQ=255)

                                             ggCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCttt                                            <  1:1574430/93‑1 (MQ=255)

                                             ggCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCttt                                            <  1:1993329/93‑1 (MQ=255)

                                               ccGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGa                                          >  1:2083191/1‑93 (MQ=255)

                                               ccGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGa                                          <  2:2172884/93‑1 (MQ=255)

                                                cgcgAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGAt                                         <  1:229004/93‑1 (MQ=255)

                                                cgcgAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGAt                                         >  2:2289071/1‑93 (MQ=255)

                                                   gagaAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGc                                      >  1:4114310/1‑93 (MQ=255)

                                                    agaAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCa                                     >  2:5361033/1‑93 (MQ=255)

                                                    agaAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCa                                     >  1:4157363/1‑93 (MQ=255)

                                                    agaAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCa                                     >  2:3232014/1‑93 (MQ=255)

                                                     gaAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGGTTGCAt                                    >  1:2241351/1‑93 (MQ=255)

                                                     gaAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCAt                                    <  1:3930577/93‑1 (MQ=255)

                                                      aaGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATc                                   >  2:2513272/1‑93 (MQ=255)

                                                        gCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCAc                                 >  2:4492083/1‑93 (MQ=255)

                                                         cTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCAcc                                <  2:198946/93‑1 (MQ=255)

                                                         cTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCAcc                                <  2:139462/93‑1 (MQ=255)

                                                            cGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCAtt                             >  2:4703964/1‑93 (MQ=255)

                                                            cGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCAtt                             >  1:357519/1‑93 (MQ=255)

                                                             gTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCAttt                            >  1:5947560/1‑93 (MQ=255)

                                                             gTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCAttt                            >  2:885868/1‑93 (MQ=255)

                                                             gTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCAttt                            >  2:1675757/1‑93 (MQ=255)

                                                               aaTATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCg                          >  1:4667649/1‑93 (MQ=255)

                                                                 tatCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGtt                        <  2:4853340/93‑1 (MQ=255)

                                                                  atCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGttt                       <  2:6193602/93‑1 (MQ=255)

                                                                   tCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTg                      >  2:46069/1‑93 (MQ=255)

                                                                   tCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTg                      >  1:329581/1‑93 (MQ=255)

                                                                    cGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTgg                     >  1:4398422/1‑93 (MQ=255)

                                                                     ggTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGt                    <  2:5713827/93‑1 (MQ=255)

                                                                     ggTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGt                    <  2:6403227/93‑1 (MQ=255)

                                                                     ggTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGt                    >  1:520166/1‑93 (MQ=255)

                                                                     ggTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGt                    <  2:6349021/93‑1 (MQ=255)

                                                                        gtgATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTg                 >  1:6190032/1‑93 (MQ=255)

                                                                        gtgATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTg                 <  2:783294/93‑1 (MQ=255)

                                                                         tgATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGc                <  2:1785023/93‑1 (MQ=255)

                                                                          gATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCg               >  2:6237288/1‑93 (MQ=255)

                                                                          gATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCg               >  2:3949458/1‑93 (MQ=255)

                                                                          gATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGATGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCg               >  1:749404/1‑93 (MQ=255)

                                                                           aTTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGt              >  1:1012441/1‑93 (MQ=255)

                                                                             tGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTaa            <  2:5550766/93‑1 (MQ=255)

                                                                              ggCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAAc           <  2:2015616/93‑1 (MQ=255)

                                                                              ggCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAAc           >  2:1526938/1‑93 (MQ=255)

                                                                                  aCGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTTGTCTGCGTAACGTCa       >  1:4941219/1‑93 (MQ=255)

                                                                                     ttGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCg    <  2:4766493/93‑1 (MQ=255)

                                                                                     ttGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCg    >  2:1222251/1‑93 (MQ=255)

                                                                                     ttGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCg    >  1:1943219/1‑93 (MQ=255)

                                                                                      tGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGa   <  2:3405629/93‑1 (MQ=255)

GGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGAC  >  NC_000913/4016706‑4016885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAA  >  NC_000913/4016703‑4016887

ACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGC                                                                                             >  SRR4302534.2068372/1‑94 (MQ=60)

   GGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATG                                                                                          >  SRR4302534.4929747/1‑94 (MQ=60)

     CAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTT                                                                                        <  SRR4302534.3943304/94‑1 (MQ=60)

     CAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTT                                                                                        >  SRR4302534.3340199/1‑94 (MQ=60)

     CAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTT                                                                                        <  SRR4302534.3956379/94‑1 (MQ=60)

     CAAAGTGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTT                                                                                        <  SRR4302534.3882902/94‑1 (MQ=60)

          TGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGC                                                                                   >  SRR4302534.654507/1‑94 (MQ=60)

          TGGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGC                                                                                   >  SRR4302534.3343840/1‑94 (MQ=60)

           GGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCG                                                                                  <  SRR4302534.5103360/94‑1 (MQ=60)

           GGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCG                                                                                  <  SRR4302534.3256341/94‑1 (MQ=60)

           GGTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCG                                                                                  <  SRR4302534.4674187/94‑1 (MQ=60)

            GTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGA                                                                                 >  SRR4302534.4230811/1‑94 (MQ=60)

            GTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGA                                                                                 >  SRR4302534.3432852/1‑94 (MQ=60)

            GTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGA                                                                                 >  SRR4302534.223602/1‑94 (MQ=60)

            GTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGA                                                                                 >  SRR4302534.5092455/1‑94 (MQ=60)

            GTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGA                                                                                 <  SRR4302534.5611347/94‑1 (MQ=60)

            GTCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGA                                                                                 >  SRR4302534.1547939/1‑94 (MQ=60)

             TCCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAA                                                                                >  SRR4302534.3805997/1‑94 (MQ=60)

              CCTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCCGGCGAAC                                                                               >  SRR4302534.590461/1‑94 (MQ=60)

               CTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACG                                                                              >  SRR4302534.743249/1‑94 (MQ=60)

               CTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACG                                                                              <  SRR4302534.2866631/94‑1 (MQ=60)

               CTTGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACG                                                                              <  SRR4302534.5927588/94‑1 (MQ=60)

                 TGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCT                                                                            <  SRR4302534.677266/94‑1 (MQ=60)

                 TGCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCT                                                                            <  SRR4302534.5585083/94‑1 (MQ=60)

                  GCTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTG                                                                           <  SRR4302534.5821142/94‑1 (MQ=60)

                   CTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGA                                                                          >  SRR4302534.6325615/1‑94 (MQ=60)

                   CTGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGA                                                                          <  SRR4302534.1564545/94‑1 (MQ=60)

                    TGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAG                                                                         <  SRR4302534.4045694/94‑1 (MQ=60)

                    TGATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCCTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAG                                                                         >  SRR4302534.546279/1‑94 (MQ=60)

                     GATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGG                                                                        >  SRR4302534.1294116/1‑94 (MQ=60)

                     GATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGGTCAGGCGAACGCTGAGG                                                                        >  SRR4302534.1884153/1‑94 (MQ=60)

                      ATATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGC                                                                       <  SRR4302534.527515/94‑1 (MQ=60)

                       TATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCG                                                                      <  SRR4302534.2963620/94‑1 (MQ=60)

                       GATCAATGAATCCATGCCGAATATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTAAGGCG                                                                      <  SRR4302534.5079004/94‑1 (MQ=60)

                        ATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGC                                                                     >  SRR4302534.5220113/1‑94 (MQ=60)

                        ATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGC                                                                     >  SRR4302534.82871/1‑94 (MQ=60)

                        ATCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGC                                                                     >  SRR4302534.1026014/1‑94 (MQ=60)

                         TCAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCT                                                                    <  SRR4302534.2091801/94‑1 (MQ=60)

                          CAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTG                                                                   >  SRR4302534.877443/1‑94 (MQ=60)

                          CAATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTG                                                                   >  SRR4302534.4319929/1‑94 (MQ=60)

                            ATGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCATGCGAACGCTGAGGCGCTGCC                                                                 <  SRR4302534.6408353/94‑1 (MQ=60)

                             TGAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCG                                                                <  SRR4302534.3052292/94‑1 (MQ=60)

                              GAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGT                                                               >  SRR4302534.2070536/1‑94 (MQ=60)

                              GAATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGT                                                               >  SRR4302534.5847779/1‑94 (MQ=60)

                               AATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAAGGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTT                                                              <  SRR4302534.6318301/94‑1 (MQ=60)

                               AATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTT                                                              <  SRR4302534.5428883/94‑1 (MQ=60)

                               AATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTT                                                              <  SRR4302534.1533972/94‑1 (MQ=60)

                                ATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTC                                                             <  SRR4302534.5225854/94‑1 (MQ=60)

                                ATCCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTC                                                             >  SRR4302534.3224351/1‑94 (MQ=60)

                                  CCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCCACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCC                                                           >  SRR4302534.6546924/1‑94 (MQ=60)

                                  CCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCC                                                           <  SRR4302534.3987145/94‑1 (MQ=60)

                                  CCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCC                                                           >  SRR4302534.1721371/1‑94 (MQ=60)

                                  CCATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCC                                                           >  SRR4302534.2577120/1‑94 (MQ=60)

                                   CATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCG                                                          <  SRR4302534.2807604/94‑1 (MQ=60)

                                    ATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGG                                                         >  SRR4302534.1738384/1‑94 (MQ=60)

                                    ATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGG                                                         >  SRR4302534.5833823/1‑94 (MQ=60)

                                    ATGCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGG                                                         >  SRR4302534.2799697/1‑94 (MQ=60)

                                      GCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGAT                                                       <  SRR4302534.4866523/94‑1 (MQ=60)

                                      GCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGAT                                                       <  SRR4302534.1295507/94‑1 (MQ=60)

                                      GCTCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGAT                                                       <  SRR4302534.84151/94‑1 (MQ=60)

                                        TCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGTTGAGGCGCTGCCGTTCCCGGATAA                                                     <  SRR4302534.5321881/94‑1 (MQ=60)

                                        TCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAA                                                     <  SRR4302534.808092/94‑1 (MQ=60)

                                        TCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAA                                                     <  SRR4302534.2417689/94‑1 (MQ=60)

                                         CAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAAC                                                    <  SRR4302534.805340/94‑1 (MQ=60)

                                          AAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACA                                                   >  SRR4302534.1763099/1‑94 (MQ=60)

                                           AAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACAC                                                  >  SRR4302534.4239541/1‑94 (MQ=60)

                                           AAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACAC                                                  >  SRR4302534.4405893/1‑94 (MQ=60)

                                             ATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCT                                                <  SRR4302534.4374805/94‑1 (MQ=60)

                                             ATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCT                                                <  SRR4302534.5198867/94‑1 (MQ=60)

                                               GGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTT                                              <  SRR4302534.4929739/94‑1 (MQ=60)

                                                GGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTG                                             <  SRR4302534.1615635/94‑1 (MQ=60)

                                                GGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTG                                             <  SRR4302534.2045541/94‑1 (MQ=60)

                                                 GCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGA                                            >  SRR4302534.2137766/1‑94 (MQ=60)

                                                  CCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGAT                                           >  SRR4302534.1641410/1‑94 (MQ=60)

                                                   CGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATT                                          <  SRR4302534.234988/94‑1 (MQ=60)

                                                   CGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATT                                          >  SRR4302534.2037484/1‑94 (MQ=60)

                                                    GCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTG                                         <  SRR4302534.3223394/94‑1 (MQ=60)

                                                     CGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGC                                        >  SRR4302534.4224996/1‑94 (MQ=60)

                                                     CGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGC                                        >  SRR4302534.3119365/1‑94 (MQ=60)

                                                      GAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCA                                       >  SRR4302534.2309020/1‑94 (MQ=60)

                                                      GAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCA                                       >  SRR4302534.5936705/1‑94 (MQ=60)

                                                      GAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCA                                       >  SRR4302534.4269626/1‑94 (MQ=60)

                                                       CGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGGTTGCAT                                      >  SRR4302534.2300751/1‑94 (MQ=60)

                                                        GAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATC                                     <  SRR4302534.4035864/94‑1 (MQ=60)

                                                        GAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATC                                     >  SRR4302534.230472/1‑94 (MQ=60)

                                                            CTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCA                                 <  SRR4302534.5449927/94‑1 (MQ=60)

                                                            CTGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCA                                 >  SRR4302534.4827313/1‑94 (MQ=60)

                                                            CGGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCA                                 >  SRR4302534.6369483/1‑94 (MQ=60)

                                                             TGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCAT                                >  SRR4302534.843669/1‑94 (MQ=60)

                                                             TGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCAT                                >  SRR4302534.1739382/1‑94 (MQ=60)

                                                             TGCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCAT                                <  SRR4302534.3834695/94‑1 (MQ=60)

                                                              GCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATT                               <  SRR4302534.18861/94‑1 (MQ=60)

                                                              GCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATT                               >  SRR4302534.366656/1‑94 (MQ=60)

                                                              GCGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATT                               <  SRR4302534.1860205/94‑1 (MQ=60)

                                                               CGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTT                              >  SRR4302534.6109055/1‑94 (MQ=60)

                                                               CGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTT                              >  SRR4302534.4453772/1‑94 (MQ=60)

                                                               CGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTT                              <  SRR4302534.5954934/94‑1 (MQ=60)

                                                               CGTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTT                              <  SRR4302534.6126749/94‑1 (MQ=60)

                                                                GTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTC                             >  SRR4302534.3713084/1‑94 (MQ=60)

                                                                GTAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTC                             <  SRR4302534.796080/94‑1 (MQ=60)

                                                                 TAATATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCG                            >  SRR4302534.4794367/1‑94 (MQ=60)

                                                                    TATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTT                         <  SRR4302534.1881874/94‑1 (MQ=60)

                                                                    TATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTT                         <  SRR4302534.1031952/94‑1 (MQ=60)

                                                                    TATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTT                         >  SRR4302534.2880402/1‑94 (MQ=60)

                                                                    TATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTT                         >  SRR4302534.4965663/1‑94 (MQ=60)

                                                                     ATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTG                        >  SRR4302534.338046/1‑94 (MQ=60)

                                                                     ATCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTG                        <  SRR4302534.2418553/94‑1 (MQ=60)

                                                                      TCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGG                       <  SRR4302534.2016007/94‑1 (MQ=60)

                                                                      TCGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGG                       >  SRR4302534.4517097/1‑94 (MQ=60)

                                                                       CGGTGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGT                      >  SRR4302534.533099/1‑94 (MQ=60)

                                                                          TGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTG                   >  SRR4302534.6358482/1‑94 (MQ=60)

                                                                          TGTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTG                   <  SRR4302534.1106467/94‑1 (MQ=60)

                                                                           GTGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGC                  >  SRR4302534.4554289/1‑94 (MQ=60)

                                                                            TGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCG                 <  SRR4302534.3380490/94‑1 (MQ=60)

                                                                            TGATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGATGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCG                 >  SRR4302534.767972/1‑94 (MQ=60)

                                                                             GATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGT                >  SRR4302534.1038494/1‑94 (MQ=60)

                                                                              ATTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTA               <  SRR4302534.2413281/94‑1 (MQ=60)

                                                                              ATTGGCAACGTTGAGCATGTGCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACCCCTTTGATTGCATCCCCATTTCGTTTGGTCTGCGTA               <  SRR4302534.548741/94‑1 (MQ=60)

                                                                               TTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAA              >  SRR4302534.2225544/1‑94 (MQ=60)

                                                                               TTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAA              >  SRR4302534.5283474/1‑94 (MQ=60)

                                                                               TTGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAA              <  SRR4302534.1321180/94‑1 (MQ=60)

                                                                                TGGCAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAAC             >  SRR4302534.3721844/1‑94 (MQ=60)

                                                                                   CAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCTCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTC          <  SRR4302534.5174147/94‑1 (MQ=60)

                                                                                   CAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTC          <  SRR4302534.2579938/94‑1 (MQ=60)

                                                                                   CAACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTC          >  SRR4302534.3369729/1‑94 (MQ=60)

                                                                                    AACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTTGTCTGCGTAACGTCA         >  SRR4302534.5075090/1‑94 (MQ=60)

                                                                                    AACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCC         >  SRR4302534.6194877/1‑94 (MQ=60)

                                                                                    AACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCA         >  SRR4302534.6531057/1‑94 (MQ=60)

                                                                                    AACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCA         >  SRR4302534.6374505/1‑94 (MQ=60)

                                                                                    AACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCA         >  SRR4302534.2113678/1‑94 (MQ=60)

                                                                                    AACGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCA         <  SRR4302534.2977517/94‑1 (MQ=60)

                                                                                    AACGTTGAGCATGGTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCA         >  SRR4302534.2399697/1‑94 (MQ=60)

                                                                                      CGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACC       >  SRR4302534.206222/1‑94 (MQ=60)

                                                                                      CGTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGACTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACC       >  SRR4302534.1718295/1‑94 (MQ=60)

                                                                                       GTTGAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCG      >  SRR4302534.1994040/1‑94 (MQ=60)

                                                                                          GAGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACA   >  SRR4302534.4027802/1‑94 (MQ=60)

                                                                                           AGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAA  >  SRR4302534.2548997/1‑94 (MQ=60)

                                                                                           AGCATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAA  >  SRR4302534.5520116/1‑94 (MQ=60)

ACTGGCAAAGTGGTCCTTGCTGATATCAATGAATCCATGCCCAAAATGGGCCGCGAGAAGCTGCGTAATATCGGTGTGATTGGCAACGTTGAGTATGTTCAGGCGAACGCTGAGGCGCTGCCGTTCCCGGATAACACCTTTGATTGCATCACCATTTCGTTTGGTCTGCGTAACGTCACCGACAA  >  NC_000913/4016703‑4016887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`