Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,028,399 G→A L55L (CTG→TTG)  fadB ← 4‑enzyme protein: 3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,028,3990GA99.6% 914.0 / ‑5.5 253L55L (CTG→TTG) fadB4‑enzyme protein: 3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (119/133);  total (119/134)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 281 total aligned reads displayed.

TTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTG  >  NC_000913/4028309‑4028488
                                                                                          |                                                                                         
 tAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAAccc                                                                                        <  1:93727/93‑1 (MQ=255)
  aaCTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCt                                                                                       >  1:5482886/1‑93 (MQ=255)
  aaCTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCt                                                                                       >  2:5241850/1‑93 (MQ=255)
   aCTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCtt                                                                                      >  1:2915171/1‑93 (MQ=255)
    cTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCttt                                                                                     >  1:6162328/1‑93 (MQ=255)
     tGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCtttt                                                                                    <  2:426013/93‑1 (MQ=255)
      gTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTa                                                                                   <  2:3285755/93‑1 (MQ=255)
       ttcttcAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAg                                                                                  <  2:5528536/93‑1 (MQ=255)
        tcttcAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGa                                                                                 <  2:378639/93‑1 (MQ=255)
        tcttcAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGa                                                                                 >  2:5092583/1‑93 (MQ=255)
         cttcAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGAt                                                                                <  1:3046982/93‑1 (MQ=255)
          ttcAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATc                                                                               <  2:2913277/93‑1 (MQ=255)
          ttcAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATc                                                                               <  1:5974643/93‑1 (MQ=255)
           tcAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCt                                                                              <  1:1525512/93‑1 (MQ=255)
            cAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTg                                                                             >  1:6303701/1‑93 (MQ=255)
            cAGGAACGAGGAACAGAGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTg                                                                             <  1:1966502/93‑1 (MQ=255)
              ggAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGAt                                                                           <  2:6087754/93‑1 (MQ=255)
               gAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGAtt                                                                          >  2:2702579/1‑93 (MQ=255)
               gAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGAtt                                                                          <  2:2284261/93‑1 (MQ=255)
                 aCGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGc                                                                        >  2:1778069/1‑93 (MQ=255)
                  cGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCt                                                                       <  1:1469673/93‑1 (MQ=255)
                    aGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGt                                                                     >  2:4773284/1‑93 (MQ=255)
                    aGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGt                                                                     >  2:3098827/1‑93 (MQ=255)
                     ggAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGtt                                                                    >  1:5549626/1‑93 (MQ=255)
                     ggAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGtt                                                                    >  1:3208733/1‑93 (MQ=255)
                     ggAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGtt                                                                    >  1:4253519/1‑93 (MQ=255)
                      gAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTc                                                                   <  2:187176/93‑1 (MQ=255)
                      gAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTc                                                                   <  2:4961611/93‑1 (MQ=255)
                       aaCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTcc                                                                  <  1:5292855/93‑1 (MQ=255)
                        aCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCa                                                                 >  2:1691230/1‑93 (MQ=255)
                        aCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCa                                                                 >  2:116765/1‑93 (MQ=255)
                        aCAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCa                                                                 <  2:3671855/93‑1 (MQ=255)
                           gggACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCa                                                              >  1:2515432/1‑93 (MQ=255)
                           gggACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCa                                                              <  2:2013565/93‑1 (MQ=255)
                            ggACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCAc                                                             <  1:2154793/93‑1 (MQ=255)
                             gACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACg                                                            >  2:2413708/1‑93 (MQ=255)
                              aCAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGc                                                           <  1:780183/93‑1 (MQ=255)
                              aCAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGc                                                           >  1:394320/1‑93 (MQ=255)
                               cAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGcc                                                          >  1:3088904/1‑93 (MQ=255)
                                 aaaaTTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGa                                                        >  2:4173683/1‑93 (MQ=255)
                                  aaaTTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGAt                                                       <  2:4647359/93‑1 (MQ=255)
                                  aaaTTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGAt                                                       >  1:592801/1‑93 (MQ=255)
                                  aaaTTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGAt                                                       <  2:2541750/93‑1 (MQ=255)
                                   aaTTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATg                                                      <  1:3136856/93‑1 (MQ=255)
                                   aaTTCGGTGATAGCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATg                                                      <  1:3247525/93‑1 (MQ=255)
                                    aTTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATgg                                                     >  1:5624306/1‑93 (MQ=255)
                                    aTTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATgg                                                     >  2:2962427/1‑93 (MQ=255)
                                    aTTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATgg                                                     <  1:1754122/93‑1 (MQ=255)
                                       cGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCt                                                  <  1:5814488/93‑1 (MQ=255)
                                       cGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCt                                                  <  1:172211/93‑1 (MQ=255)
                                             tatCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGa                                            >  2:1908920/1‑93 (MQ=255)
                                             tatCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGa                                            <  2:5727574/93‑1 (MQ=255)
                                              atCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAg                                           >  2:882815/1‑93 (MQ=255)
                                               tCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAgg                                          >  1:1231737/1‑93 (MQ=255)
                                                cAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGc                                         <  1:6398633/93‑1 (MQ=255)
                                                 aGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCt                                        <  2:752750/93‑1 (MQ=255)
                                                 aGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCt                                        <  1:5707654/93‑1 (MQ=255)
                                                 aGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCt                                        <  1:5890374/93‑1 (MQ=255)
                                                  gCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTg                                       >  2:4833218/1‑93 (MQ=255)
                                                   cACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTgg                                      <  2:1314461/93‑1 (MQ=255)
                                                   cACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTgg                                      <  1:4178401/93‑1 (MQ=255)
                                                    cccGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGc                                     <  1:2401892/92‑1 (MQ=255)
                                                    aCCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGc                                     >  2:1217927/1‑93 (MQ=255)
                                                       gacgaTAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGAc                                  >  2:62226/1‑93 (MQ=255)
                                                       gacgaTAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGAc                                  >  1:3571325/1‑93 (MQ=255)
                                                       gacgaTAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGAc                                  >  2:4506796/1‑93 (MQ=255)
                                                       gacgaTAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGAc                                  <  1:3716217/93‑1 (MQ=255)
                                                          gaTAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGt                               >  2:3187766/1‑93 (MQ=255)
                                                            tAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCg                             <  1:34612/93‑1 (MQ=255)
                                                              aaGGCTGCTTTGTTCGAGCGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCa                           <  2:153058/93‑1 (MQ=255)
                                                              aaGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCa                           <  1:6254708/93‑1 (MQ=255)
                                                               aGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAg                          <  2:1425414/93‑1 (MQ=255)
                                                                ggCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAgt                         >  1:264574/1‑93 (MQ=255)
                                                                ggCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAgt                         <  1:1025376/93‑1 (MQ=255)
                                                                ggCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAgt                         >  1:4005738/1‑93 (MQ=255)
                                                                ggCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAgt                         >  1:4568870/1‑93 (MQ=255)
                                                                  ctgctTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAgtgt                       >  2:6424561/1‑93 (MQ=255)
                                                                  ctgctTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAgtgt                       <  2:2594086/93‑1 (MQ=255)
                                                                   tgctTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTc                      >  2:5398182/1‑93 (MQ=255)
                                                                    gctTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCg                     >  2:4206164/1‑93 (MQ=255)
                                                                    gctTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCg                     <  1:5031027/93‑1 (MQ=255)
                                                                    gctTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCg                     >  1:2055306/1‑93 (MQ=255)
                                                                    gctTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCg                     >  2:515942/1‑93 (MQ=255)
                                                                       ttGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGt                  <  2:6268697/93‑1 (MQ=255)
                                                                        tGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGtt                 <  1:383731/93‑1 (MQ=255)
                                                                        tGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGtt                 <  2:2650857/93‑1 (MQ=255)
                                                                         gTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGCGttt                >  1:1107525/1‑93 (MQ=255)
                                                                         gTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGttt                <  2:5816465/93‑1 (MQ=255)
                                                                          ttCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTtta               <  1:4034258/93‑1 (MQ=255)
                                                                           tCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTttat              <  2:2082085/93‑1 (MQ=255)
                                                                               aCGCAGCACCACCCCTTTAAGATCTTATTGCTGTCCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAAc          <  1:5464742/93‑1 (MQ=255)
                                                                                 gcagcagcaACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTg        >  2:4013721/1‑93 (MQ=255)
                                                                                  cagcagcaaCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGa       <  1:4763314/93‑1 (MQ=255)
                                                                                  cagcagcaaCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGa       <  2:6436165/93‑1 (MQ=255)
                                                                                   agcagcaacCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGaa      >  1:3962984/1‑93 (MQ=255)
                                                                                   agcagcaacCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGaa      >  1:23460/1‑93 (MQ=255)
                                                                                   agcagcaacCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGaa      <  1:4503257/93‑1 (MQ=255)
                                                                                    gcagcaacCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAAc     >  2:2774030/1‑93 (MQ=255)
                                                                                    gcagcaacCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAAc     >  1:6054081/1‑93 (MQ=255)
                                                                                     cagcaccCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAAcc    <  1:4394083/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTG  >  NC_000913/4028309‑4028488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGC  >  NC_000913/4028306‑4028492
                                                                                             |                                                                                             
GACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAA                                                                                               >  SRR4302534.6376693/1‑94 (MQ=60)
GACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAA                                                                                               <  SRR4302534.1365585/94‑1 (MQ=60)
  CTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACC                                                                                             >  SRR4302534.1203054/1‑94 (MQ=60)
    TAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCT                                                                                           <  SRR4302534.96232/94‑1 (MQ=60)
    TAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCT                                                                                           >  SRR4302534.5630747/1‑94 (MQ=60)
     AACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTT                                                                                          >  SRR4302534.2991680/1‑94 (MQ=60)
     AACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTT                                                                                          <  SRR4302534.2137140/94‑1 (MQ=60)
      ACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTT                                                                                         >  SRR4302534.6329944/1‑94 (MQ=60)
       CTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTT                                                                                        <  SRR4302534.3252950/94‑1 (MQ=60)
        TGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTA                                                                                       <  SRR4302534.142352/94‑1 (MQ=60)
         GTTCTTCAGGAACGAGGAACAGGTACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAG                                                                                      <  SRR4302534.3940183/94‑1 (MQ=60)
         GTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAA                                                                                      >  SRR4302534.6320369/1‑94 (MQ=60)
          TTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGA                                                                                     <  SRR4302534.1745125/94‑1 (MQ=60)
            CTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATC                                                                                   <  SRR4302534.3126909/94‑1 (MQ=60)
            CTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATC                                                                                   <  SRR4302534.5538701/94‑1 (MQ=60)
            CTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATC                                                                                   <  SRR4302534.4086227/94‑1 (MQ=60)
            CTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATC                                                                                   <  SRR4302534.6034340/94‑1 (MQ=60)
             TTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCT                                                                                  <  SRR4302534.6136910/94‑1 (MQ=60)
             TTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCT                                                                                  >  SRR4302534.766849/1‑94 (MQ=60)
              TCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTG                                                                                 <  SRR4302534.1565563/94‑1 (MQ=60)
              TCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTG                                                                                 >  SRR4302534.6474166/1‑94 (MQ=60)
               CAGGAACGAGGAACAGAGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGA                                                                                <  SRR4302534.2017974/94‑1 (MQ=60)
                AGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGAT                                                                               >  SRR4302534.3328305/1‑94 (MQ=60)
                 GGAGCGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATT                                                                              <  SRR4302534.4367459/94‑1 (MQ=60)
                 GGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATT                                                                              <  SRR4302534.6126053/94‑1 (MQ=60)
                  GAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTG                                                                             <  SRR4302534.5364853/94‑1 (MQ=60)
                  GAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACACTTTTAGCTCTGATTG                                                                             >  SRR4302534.263482/1‑94 (MQ=60)
                   AACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGC                                                                            >  SRR4302534.2497020/1‑94 (MQ=60)
                    ACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCT                                                                           <  SRR4302534.5844329/94‑1 (MQ=60)
                     CGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTG                                                                          <  SRR4302534.1508336/94‑1 (MQ=60)
                      GAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGT                                                                         >  SRR4302534.3012164/1‑94 (MQ=60)
                       AGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTT                                                                        >  SRR4302534.3293880/1‑94 (MQ=60)
                       AGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTT                                                                        >  SRR4302534.3694400/1‑94 (MQ=60)
                       AGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTT                                                                        >  SRR4302534.5699420/1‑94 (MQ=60)
                       AGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTT                                                                        >  SRR4302534.4745643/1‑94 (MQ=60)
                       AGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTT                                                                        >  SRR4302534.4368297/1‑94 (MQ=60)
                        GGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTC                                                                       <  SRR4302534.6388336/94‑1 (MQ=60)
                        GGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTC                                                                       >  SRR4302534.3007469/1‑94 (MQ=60)
                          AACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCA                                                                     <  SRR4302534.3294257/94‑1 (MQ=60)
                          AACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCA                                                                     <  SRR4302534.5435732/94‑1 (MQ=60)
                             AGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCA                                                                  >  SRR4302534.2582226/1‑94 (MQ=60)
                               GGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACG                                                                <  SRR4302534.2211674/94‑1 (MQ=60)
                               GGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACG                                                                <  SRR4302534.5132464/94‑1 (MQ=60)
                                GACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGC                                                               >  SRR4302534.404401/1‑94 (MQ=60)
                                GACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGC                                                               <  SRR4302534.4302753/94‑1 (MQ=60)
                                 ACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCC                                                              <  SRR4302534.1087494/94‑1 (MQ=60)
                                 ACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCC                                                              >  SRR4302534.3935150/1‑94 (MQ=60)
                                 ACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCC                                                              >  SRR4302534.3170113/1‑94 (MQ=60)
                                 ACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCC                                                              <  SRR4302534.799635/94‑1 (MQ=60)
                                 ACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCC                                                              <  SRR4302534.1559106/94‑1 (MQ=60)
                                   AAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGA                                                            <  SRR4302534.2063354/94‑1 (MQ=60)
                                   AAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGA                                                            >  SRR4302534.2406236/1‑94 (MQ=60)
                                    AAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGAT                                                           >  SRR4302534.607276/1‑94 (MQ=60)
                                    AAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGAT                                                           <  SRR4302534.3965814/94‑1 (MQ=60)
                                     AAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATG                                                          <  SRR4302534.5643733/94‑1 (MQ=60)
                                     AAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATG                                                          >  SRR4302534.4039534/1‑94 (MQ=60)
                                     AAATGCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATG                                                          <  SRR4302534.5970637/94‑1 (MQ=60)
                                      AATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGG                                                         <  SRR4302534.3219349/94‑1 (MQ=60)
                                      AATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGG                                                         >  SRR4302534.5776509/1‑94 (MQ=60)
                                      AATTCGGTGATAGCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGG                                                         <  SRR4302534.3333999/94‑1 (MQ=60)
                                       ATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGC                                                        <  SRR4302534.1799667/94‑1 (MQ=60)
                                         TCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCT                                                      <  SRR4302534.6503217/94‑1 (MQ=60)
                                          CGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTC                                                     <  SRR4302534.176753/94‑1 (MQ=60)
                                          CGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTC                                                     <  SRR4302534.5972306/94‑1 (MQ=60)
                                           GGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCG                                                    >  SRR4302534.6336428/1‑94 (MQ=60)
                                           GGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCG                                                    >  SRR4302534.6072391/1‑94 (MQ=60)
                                            GTGATATCAGCACCGACGATAAAGGCTGCTTTGGTCGAACGCAGCAGCAAGCCTTGTAGATCTGATTGCTGTTCCAGAACGCCGATGGGATCGC                                                   >  SRR4302534.6622296/1‑94 (MQ=60)
                                               ATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGA                                                <  SRR4302534.6428000/94‑1 (MQ=60)
                                               ATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGA                                                >  SRR4302534.3605960/1‑94 (MQ=60)
                                                 ATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGG                                              >  SRR4302534.1264168/1‑94 (MQ=60)
                                                  TCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGCGGC                                             >  SRR4302534.4411043/1‑94 (MQ=60)
                                                  TCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGC                                             >  SRR4302534.863227/1‑94 (MQ=60)
                                                   CAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCT                                            <  SRR4302534.6571431/94‑1 (MQ=60)
                                                    AGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAATCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATCGCCTCGCCGAGGCTG                                           >  SRR4302534.1562331/1‑94 (MQ=60)
                                                    AGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTG                                           >  SRR4302534.576468/1‑94 (MQ=60)
                                                    AGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTG                                           <  SRR4302534.5862273/94‑1 (MQ=60)
                                                    AGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTG                                           >  SRR4302534.2419793/1‑94 (MQ=60)
                                                    AGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTG                                           <  SRR4302534.6050312/94‑1 (MQ=60)
                                                    AGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTG                                           <  SRR4302534.6203146/94‑1 (MQ=60)
                                                     cccccgaagaaAAAGGCTGTTTTGTTCAACCGCAGCAGCACCCTTTTTAGTTTTGATTGCTTTTCCGGCACGCCGATGGCCTCGCCGAGGCTGG                                          <  SRR4302534.4556857/83‑1 (MQ=33)
                                                      CACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGC                                         >  SRR4302534.3151012/1‑94 (MQ=60)
                                                      CACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGC                                         <  SRR4302534.4291291/94‑1 (MQ=60)
                                                       CCCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCG                                        <  SRR4302534.2465732/94‑1 (MQ=60)
                                                         CGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGAC                                      <  SRR4302534.4772974/94‑1 (MQ=60)
                                                         CGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGAC                                      >  SRR4302534.3667386/1‑94 (MQ=60)
                                                          GACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACG                                     <  SRR4302534.4153383/94‑1 (MQ=60)
                                                          GACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACG                                     <  SRR4302534.2921616/94‑1 (MQ=60)
                                                          GACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACG                                     <  SRR4302534.3816133/94‑1 (MQ=60)
                                                              ATAAAGGCTGCTTTGTTCGAACGCAGCAGCATCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCG                                 <  SRR4302534.1452462/94‑1 (MQ=60)
                                                              ATAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCG                                 >  SRR4302534.972385/1‑94 (MQ=60)
                                                               TAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGC                                >  SRR4302534.3390977/1‑94 (MQ=60)
                                                               TAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGC                                <  SRR4302534.35523/94‑1 (MQ=60)
                                                               TAAAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGC                                >  SRR4302534.5280901/1‑94 (MQ=60)
                                                                 AAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGGGACGGTCGCAG                              >  SRR4302534.5688854/1‑94 (MQ=60)
                                                                 AAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAG                              >  SRR4302534.1779757/1‑94 (MQ=60)
                                                                 AAGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAG                              <  SRR4302534.6424240/94‑1 (MQ=60)
                                                                  AGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGT                             <  SRR4302534.6500196/94‑1 (MQ=60)
                                                                  AGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGT                             >  SRR4302534.4616798/1‑94 (MQ=60)
                                                                  AGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGT                             >  SRR4302534.271441/1‑94 (MQ=60)
                                                                  AGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGT                             >  SRR4302534.4692734/1‑94 (MQ=60)
                                                                  AGGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGT                             >  SRR4302534.4112992/1‑94 (MQ=60)
                                                                   GGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTG                            <  SRR4302534.1051788/94‑1 (MQ=60)
                                                                   GGCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTG                            <  SRR4302534.1669413/94‑1 (MQ=60)
                                                                    GCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGT                           <  SRR4302534.2024052/94‑1 (MQ=60)
                                                                    GCTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGT                           <  SRR4302534.4216047/94‑1 (MQ=60)
                                                                     CTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTC                          >  SRR4302534.4071901/1‑94 (MQ=60)
                                                                     CTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTC                          <  SRR4302534.2672130/94‑1 (MQ=60)
                                                                     CTGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTC                          <  SRR4302534.6235466/94‑1 (MQ=60)
                                                                      TGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCG                         >  SRR4302534.2109000/1‑94 (MQ=60)
                                                                      TGCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCG                         >  SRR4302534.3436062/1‑94 (MQ=60)
                                                                       GCTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGA                        <  SRR4302534.5166920/94‑1 (MQ=60)
                                                                        CTTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAG                       <  SRR4302534.2176399/94‑1 (MQ=60)
                                                                         TTTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGT                      <  SRR4302534.5725725/94‑1 (MQ=60)
                                                                          TTGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTT                     <  SRR4302534.3584621/94‑1 (MQ=60)
                                                                           TGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGCGTTT                    >  SRR4302534.1136401/1‑94 (MQ=60)
                                                                           TGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTT                    <  SRR4302534.393541/94‑1 (MQ=60)
                                                                           TGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTT                    >  SRR4302534.1187661/1‑94 (MQ=60)
                                                                           TGTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTT                    >  SRR4302534.4598727/1‑94 (MQ=60)
                                                                            GTTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTA                   >  SRR4302534.3209780/1‑94 (MQ=60)
                                                                             TTCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTAT                  <  SRR4302534.4142405/94‑1 (MQ=60)
                                                                              TCGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATT                 <  SRR4302534.222804/94‑1 (MQ=60)
                                                                               CGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTA                >  SRR4302534.5723101/1‑94 (MQ=60)
                                                                               CGAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTA                >  SRR4302534.5969263/1‑94 (MQ=60)
                                                                                GAACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAA               >  SRR4302534.834803/1‑94 (MQ=60)
                                                                                  ACGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACT             <  SRR4302534.1402366/94‑1 (MQ=60)
                                                                                  ACGCAGCACCACCCCTTTAAGATCTTATTGCTGTCCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACT             <  SRR4302534.5612049/94‑1 (MQ=60)
                                                                                   CGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTG            >  SRR4302534.5122576/1‑94 (MQ=60)
                                                                                   CGCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTG            >  SRR4302534.2954332/1‑94 (MQ=60)
                                                                                   CGCAGCAGCAACCCTTTTAGATCGGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTG            <  SRR4302534.5617917/94‑1 (MQ=60)
                                                                                    GCAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGA           <  SRR4302534.5995050/94‑1 (MQ=60)
                                                                                     CAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAA          <  SRR4302534.4892650/94‑1 (MQ=60)
                                                                                     CAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAA          >  SRR4302534.24084/1‑94 (MQ=60)
                                                                                     CAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAA          >  SRR4302534.4069083/1‑94 (MQ=60)
                                                                                     CAGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAA          >  SRR4302534.4069081/1‑94 (MQ=60)
                                                                                      AGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAAC         <  SRR4302534.4625140/94‑1 (MQ=60)
                                                                                      AGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAAC         >  SRR4302534.3287426/1‑94 (MQ=60)
                                                                                      AGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAAC         >  SRR4302534.1973266/1‑94 (MQ=60)
                                                                                      AGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAAC         >  SRR4302534.4124095/1‑94 (MQ=60)
                                                                                      AGCAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAAC         >  SRR4302534.6218713/1‑94 (MQ=60)
                                                                                        CAGCACCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCT       <  SRR4302534.4512588/94‑1 (MQ=60)
                                                                                        CAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCT       >  SRR4302534.6154767/1‑94 (MQ=60)
                                                                                        CAGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCT       >  SRR4302534.4666465/1‑94 (MQ=60)
                                                                                         AGCAACCTTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTG      <  SRR4302534.5570434/94‑1 (MQ=60)
                                                                                         AGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTG      >  SRR4302534.6125472/1‑94 (MQ=60)
                                                                                         AGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTG      >  SRR4302534.2511719/1‑94 (MQ=60)
                                                                                         AGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTG      >  SRR4302534.3175676/1‑94 (MQ=60)
                                                                                         AGCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGCTTATTAACTGAACCCG      >  SRR4302534.4063261/1‑94 (MQ=60)
                                                                                          GCAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGG     <  SRR4302534.4511253/94‑1 (MQ=60)
                                                                                           CAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGGCTCGCCGAGGCTGGCGACCGCCGCAGAGTCGAGTTTTTTAACTGAACCTTGG    >  SRR4302534.2736061/1‑94 (MQ=60)
                                                                                           CAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGG    <  SRR4302534.2711568/94‑1 (MQ=60)
                                                                                           CAACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGG    <  SRR4302534.1100845/94‑1 (MQ=60)
                                                                                            AACCCTTTTAGATCTGATTGCTGTTGCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGG   >  SRR4302534.6395458/1‑94 (MQ=60)
                                                                                            AACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGG   <  SRR4302534.3637627/94‑1 (MQ=60)
                                                                                             ACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGC  >  SRR4302534.1849040/1‑94 (MQ=60)
                                                                                             ACCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGC  >  SRR4302534.3439273/1‑94 (MQ=60)
                                                                                             |                                                                                             
GACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGC  >  NC_000913/4028306‑4028492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑