Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,395,798	A→G	H270R (CAC→CGC)	mutL →	MutL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,395,798	0	A	G	100.0%	832.1 / NA	240	H270R (CAC→CGC)	mutL	MutL
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (118/122); total (118/122)

Only 100 of 269 total aligned reads displayed.

TGGCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGG  >  NC_000913/4395708‑4395887

tGGCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATcgcg                                                                                         <  1:2788118/93‑1 (MQ=255)

 ggCCTATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCgcgc                                                                                        <  2:4020383/93‑1 (MQ=255)

 ggCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCgcgc                                                                                        >  2:5119538/1‑93 (MQ=255)

 cgCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCgcgc                                                                                        >  1:4667011/2‑93 (MQ=255)

   ccGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGa                                                                                      <  1:3211903/93‑1 (MQ=255)

    cGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGAt                                                                                     <  1:119276/93‑1 (MQ=255)

     gATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATc                                                                                    >  2:2715340/1‑93 (MQ=255)

     gATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATc                                                                                    <  2:3090584/93‑1 (MQ=255)

      aTCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATcc                                                                                   >  2:2782225/1‑93 (MQ=255)

      aTCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATcc                                                                                   >  2:3146568/1‑93 (MQ=255)

       tCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCg                                                                                  >  2:304318/1‑93 (MQ=255)

       tCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCg                                                                                  >  1:973040/1‑93 (MQ=255)

        ccAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGc                                                                                 >  2:4480535/1‑93 (MQ=255)

         cAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGcc                                                                                <  1:4725031/93‑1 (MQ=255)

          aaaTCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCa                                                                               <  1:644935/93‑1 (MQ=255)

          aaaTCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCa                                                                               >  1:1847940/1‑93 (MQ=255)

           aaTCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAg                                                                              <  1:6427110/93‑1 (MQ=255)

           aaTCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAg                                                                              <  2:354282/93‑1 (MQ=255)

             tCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGc                                                                            >  2:2005161/1‑93 (MQ=255)

              cacaCCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGcc                                                                           >  1:2894840/1‑93 (MQ=255)

              cacaCCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGcc                                                                           <  2:1462683/93‑1 (MQ=255)

              cacaCCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGcc                                                                           <  1:4486581/93‑1 (MQ=255)

                caccacGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTg                                                                         >  2:703459/1‑93 (MQ=255)

                 accaccCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGc                                                                        <  1:3394887/93‑1 (MQ=255)

                   cacGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGa                                                                      <  2:1905896/93‑1 (MQ=255)

                    ccGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGaa                                                                     <  1:4448661/92‑1 (MQ=255)

                    ccGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGaa                                                                     <  1:5865850/92‑1 (MQ=255)

                    acGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGaa                                                                     >  2:4628078/1‑93 (MQ=255)

                    acGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGaa                                                                     >  2:5659008/1‑93 (MQ=255)

                     cGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAg                                                                    <  2:138173/93‑1 (MQ=255)

                      gCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGGGAAGa                                                                   <  1:3934259/93‑1 (MQ=255)

                       cccGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGAc                                                                  >  1:1004606/1‑93 (MQ=255)

                       cccGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGAc                                                                  >  1:4030663/1‑93 (MQ=255)

                       cccGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGAc                                                                  >  1:150728/1‑93 (MQ=255)

                       cccGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGAc                                                                  <  1:4638883/93‑1 (MQ=255)

                        ccGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACa                                                                 >  1:6116409/1‑93 (MQ=255)

                        ccGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACa                                                                 <  1:904916/93‑1 (MQ=255)

                         cGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACaa                                                                <  2:3005543/93‑1 (MQ=255)

                         cGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACaa                                                                >  1:272670/1‑93 (MQ=255)

                          gCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACaaa                                                               <  2:3862545/93‑1 (MQ=255)

                            aCTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGTGCGATCCGCCAGGCCTGCGAAGACAAACt                                                             >  1:3101104/1‑93 (MQ=255)

                            aCTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACt                                                             >  2:4771307/1‑93 (MQ=255)

                                gCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTgggg                                                         <  2:2065129/93‑1 (MQ=255)

                                 cAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTggggg                                                        >  1:6037084/1‑93 (MQ=255)

                                   gAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGcc                                                      <  2:1064229/93‑1 (MQ=255)

                                     aaTTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGa                                                    <  2:5558474/93‑1 (MQ=255)

                                     aaTTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGa                                                    <  2:2856706/93‑1 (MQ=255)

                                     aaTTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGa                                                    >  1:2377792/1‑93 (MQ=255)

                                     aaTTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGa                                                    >  1:4905020/1‑93 (MQ=255)

                                      aTTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGAt                                                   <  2:4336194/93‑1 (MQ=255)

                                      aTTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGAt                                                   >  1:3040868/1‑93 (MQ=255)

                                       ttCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATc                                                  <  2:2275035/93‑1 (MQ=255)

                                       ttCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATc                                                  <  1:5087778/93‑1 (MQ=255)

                                       ttCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATc                                                  <  2:2157158/93‑1 (MQ=255)

                                        tCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCa                                                 >  1:5934390/1‑93 (MQ=255)

                                         cAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAg                                                >  2:2221279/1‑93 (MQ=255)

                                         cAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAg                                                <  1:2584625/93‑1 (MQ=255)

                                          aGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGc                                               >  1:1336601/1‑93 (MQ=255)

                                             aTTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAAc                                            <  2:1623437/93‑1 (MQ=255)

                                              ttGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAAcc                                           <  1:5189874/93‑1 (MQ=255)

                                               tGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCg                                          <  2:4428987/93‑1 (MQ=255)

                                                  tACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCa                                       <  2:6271463/93‑1 (MQ=255)

                                                  tACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCa                                       >  2:2497965/1‑93 (MQ=255)

                                                     gTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCAttt                                    >  2:878631/1‑93 (MQ=255)

                                                          cGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGtgtt                               >  1:6211556/1‑93 (MQ=255)

                                                           ggTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGtgttg                              <  2:5495330/93‑1 (MQ=255)

                                                           ggTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGtgttg                              <  2:5259412/93‑1 (MQ=255)

                                                             tCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTa                            <  2:2375606/93‑1 (MQ=255)

                                                             tCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTa                            <  1:5317051/93‑1 (MQ=255)

                                                              cGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTAt                           >  1:2364446/1‑93 (MQ=255)

                                                               gCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATc                          >  1:1613677/1‑93 (MQ=255)

                                                               gCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATc                          <  2:68287/93‑1 (MQ=255)

                                                               gCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATc                          >  2:5329608/1‑93 (MQ=255)

                                                               gCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATc                          >  2:341470/1‑93 (MQ=255)

                                                                  tgatgCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTgg                       <  2:5008365/93‑1 (MQ=255)

                                                                   gatgCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGga                      <  2:3293042/93‑1 (MQ=255)

                                                                   gatgCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGga                      >  1:3507848/1‑93 (MQ=255)

                                                                    atgCGCGATCCCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGAATTTGTGTTGTATCTGgag                     <  2:6219553/93‑1 (MQ=255)

                                                                     tgCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGgaga                    >  1:5452445/1‑93 (MQ=255)

                                                                      gcgcGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGAt                   >  2:853153/1‑93 (MQ=255)

                                                                       cgcgATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATc                  >  1:3403540/1‑93 (MQ=255)

                                                                       cgcgATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATc                  <  2:6192791/93‑1 (MQ=255)

                                                                       cgcgATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATc                  <  2:4928087/93‑1 (MQ=255)

                                                                         cgATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGa                <  1:3677818/93‑1 (MQ=255)

                                                                         cgATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGa                >  2:4252946/1‑93 (MQ=255)

                                                                          gATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGAc               >  2:3789673/1‑93 (MQ=255)

                                                                          gATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGAc               <  2:3910167/93‑1 (MQ=255)

                                                                           aTCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGAcc              >  1:222392/1‑93 (MQ=255)

                                                                             cGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGCCAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCca            >  2:5164614/1‑93 (MQ=255)

                                                                              gCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCcaa           >  2:5079713/1‑92 (MQ=255)

                                                                                 tGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATc        >  2:2588500/1‑93 (MQ=255)

                                                                                 tGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATc        <  1:5259013/93‑1 (MQ=255)

                                                                                  gATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCa       >  1:209851/1‑93 (MQ=255)

                                                                                   atcaatcgCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAg      >  1:6127409/1‑93 (MQ=255)

                                                                                     caatcgCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGt    >  1:3701734/1‑93 (MQ=255)

                                                                                     caatcgCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGt    <  1:3750366/93‑1 (MQ=255)

                                                                                       atcgCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTgg  >  1:755139/1‑93 (MQ=255)

                                                                                       atcgCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTgg  >  1:2840664/1‑93 (MQ=255)

                                                                                       atcgCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTgg  >  1:2162513/1‑93 (MQ=255)

                                                                                       atcgCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTgg  >  2:1251260/1‑93 (MQ=255)

TGGCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGG  >  NC_000913/4395708‑4395887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GGGTGGCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGT  >  NC_000913/4395705‑4395891

GGGTGCCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCG                                                                                               <  SRR4302534.1907511/94‑1 (MQ=60)

   TGGCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGC                                                                                            <  SRR4302534.2861451/94‑1 (MQ=60)

   TCGCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGC                                                                                            >  SRR4302534.4793717/1‑94 (MQ=60)

    GGCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCG                                                                                           <  SRR4302534.930770/94‑1 (MQ=60)

    GGCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCG                                                                                           <  SRR4302534.6294000/94‑1 (MQ=60)

     GCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGA                                                                                          <  SRR4302534.1023625/94‑1 (MQ=60)

     GCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGA                                                                                          >  SRR4302534.3850088/1‑94 (MQ=60)

     GCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGA                                                                                          <  SRR4302534.5126327/94‑1 (MQ=60)

     GCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGA                                                                                          >  SRR4302534.6585611/1‑94 (MQ=60)

      CCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGAT                                                                                         >  SRR4302534.2854221/1‑94 (MQ=60)

      CCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGAT                                                                                         <  SRR4302534.3297131/94‑1 (MQ=60)

       CGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATC                                                                                        >  SRR4302534.6602981/1‑94 (MQ=60)

       CGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATC                                                                                        >  SRR4302534.3488935/1‑94 (MQ=60)

       CGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATC                                                                                        >  SRR4302534.4674913/1‑94 (MQ=60)

       CGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATC                                                                                        <  SRR4302534.122445/94‑1 (MQ=60)

        GATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCC                                                                                       >  SRR4302534.931182/1‑94 (MQ=60)

         ATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCG                                                                                      >  SRR4302534.997834/1‑94 (MQ=60)

         ATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCG                                                                                      <  SRR4302534.4077264/94‑1 (MQ=60)

         ATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCC                                                                                      >  SRR4302534.4900141/1‑94 (MQ=60)

            CAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCA                                                                                   <  SRR4302534.4853359/94‑1 (MQ=60)

            CAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCA                                                                                   >  SRR4302534.1896018/1‑94 (MQ=60)

             AAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAG                                                                                  <  SRR4302534.660576/94‑1 (MQ=60)

              AGTCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGGGATCCGCCAGG                                                                                 <  SRR4302534.3751632/94‑1 (MQ=60)

              AATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGG                                                                                 <  SRR4302534.6600429/94‑1 (MQ=60)

              AATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGG                                                                                 <  SRR4302534.6600428/94‑1 (MQ=60)

                TCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCC                                                                               >  SRR4302534.2970831/1‑94 (MQ=60)

                TCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCC                                                                               <  SRR4302534.2280923/94‑1 (MQ=60)

                 CACACCCCGCCCGCACTGGCAGAAATTCAGGATTGCTACGTGAACGGCCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCtgcaggcat                                                                              >  SRR4302534.4486762/1‑85 (MQ=60)

                 CACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCT                                                                              <  SRR4302534.4607976/94‑1 (MQ=60)

                 CACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCT                                                                              >  SRR4302534.2930589/1‑94 (MQ=60)

                 CACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCT                                                                              <  SRR4302534.6267249/94‑1 (MQ=60)

                  ACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTG                                                                             >  SRR4302534.5331586/1‑94 (MQ=60)

                  ACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTG                                                                             >  SRR4302534.2864508/1‑94 (MQ=60)

                    ACCACCCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCG                                                                           <  SRR4302534.3485980/94‑1 (MQ=60)

                       CCGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAG                                                                        <  SRR4302534.4568877/94‑1 (MQ=60)

                       CCGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAG                                                                        <  SRR4302534.6025125/94‑1 (MQ=60)

                         GCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGGGAAGAC                                                                      <  SRR4302534.4039643/94‑1 (MQ=60)

                         GCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGAC                                                                      >  SRR4302534.4138724/1‑94 (MQ=60)

                         GCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGAC                                                                      >  SRR4302534.774575/1‑94 (MQ=60)

                         GCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGAC                                                                      >  SRR4302534.1030375/1‑94 (MQ=60)

                         GCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGAC                                                                      >  SRR4302534.154655/1‑94 (MQ=60)

                          CCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACA                                                                     <  SRR4302534.2493058/94‑1 (MQ=60)

                          CCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACA                                                                     <  SRR4302534.4764762/94‑1 (MQ=60)

                          CCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACA                                                                     >  SRR4302534.6282795/1‑94 (MQ=60)

                           CCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAA                                                                    >  SRR4302534.279731/1‑94 (MQ=60)

                           CCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAA                                                                    <  SRR4302534.927904/94‑1 (MQ=60)

                             GCACTGGCAGAAATTCAGTACTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAAC                                                                  >  SRR4302534.6459332/1‑94 (MQ=60)

                              CACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGTGCGATCCGCCAGGCCTGCGAAGACAAACT                                                                 >  SRR4302534.3182672/1‑94 (MQ=60)

                              CACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACT                                                                 <  SRR4302534.898411/94‑1 (MQ=60)

                              CACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACT                                                                 <  SRR4302534.4344536/94‑1 (MQ=60)

                                CTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGG                                                               <  SRR4302534.3013586/94‑1 (MQ=60)

                                CTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGG                                                               >  SRR4302534.3567614/1‑94 (MQ=60)

                                  GGCAGAAATTCATTATTGCTACGTGAACGGTCGCATGATGCGCTATCGCCTTATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGG                                                             <  SRR4302534.6408686/94‑1 (MQ=60)

                                   GCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGG                                                            >  SRR4302534.6201155/1‑94 (MQ=60)

                                    CAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGC                                                           >  SRR4302534.5327202/1‑94 (MQ=60)

                                    CAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGC                                                           >  SRR4302534.2375232/1‑94 (MQ=60)

                                     AGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCC                                                          >  SRR4302534.1636731/1‑94 (MQ=60)

                                     AGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCC                                                          <  SRR4302534.4828653/94‑1 (MQ=60)

                                     AGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCC                                                          >  SRR4302534.1259650/1‑94 (MQ=60)

                                     AGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGAGCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCC                                                          <  SRR4302534.124238/94‑1 (MQ=60)

                                      GAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCG                                                         <  SRR4302534.723977/94‑1 (MQ=60)

                                       AAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGA                                                        >  SRR4302534.2440954/1‑94 (MQ=60)

                                       AAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGA                                                        <  SRR4302534.4230623/94‑1 (MQ=60)

                                       AAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGA                                                        >  SRR4302534.2703012/1‑94 (MQ=60)

                                       AAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGA                                                        >  SRR4302534.5037987/1‑94 (MQ=60)

                                        AATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGAT                                                       >  SRR4302534.6059280/1‑94 (MQ=60)

                                        AATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGAT                                                       >  SRR4302534.3120626/1‑94 (MQ=60)

                                         ATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGCTC                                                      >  SRR4302534.5004120/1‑94 (MQ=60)

                                         ATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATC                                                      <  SRR4302534.2733739/94‑1 (MQ=60)

                                         ATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATC                                                      >  SRR4302534.4601115/1‑94 (MQ=60)

                                          TTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCA                                                     <  SRR4302534.5225098/94‑1 (MQ=60)

                                          TTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCA                                                     >  SRR4302534.6095544/1‑94 (MQ=60)

                                            CAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGC                                                   <  SRR4302534.2653046/94‑1 (MQ=60)

                                            CAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGC                                                   >  SRR4302534.4675769/1‑94 (MQ=60)

                                            CAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGC                                                   >  SRR4302534.1371837/1‑94 (MQ=60)

                                             AGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCA                                                  <  SRR4302534.3868064/94‑1 (MQ=60)

                                             AGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCA                                                  <  SRR4302534.149561/94‑1 (MQ=60)

                                                ATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACC                                               <  SRR4302534.1655852/94‑1 (MQ=60)

                                                 TTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCG                                              <  SRR4302534.5329783/94‑1 (MQ=60)

                                                     TACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCAT                                          <  SRR4302534.5420377/94‑1 (MQ=60)

                                                     TACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCAT                                          >  SRR4302534.480983/1‑94 (MQ=60)

                                                     TACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCAT                                          <  SRR4302534.3736435/94‑1 (MQ=60)

                                                      ACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATT                                         >  SRR4302534.5530190/1‑94 (MQ=60)

                                                       CGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTT                                        <  SRR4302534.3825804/94‑1 (MQ=60)

                                                       CGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTT                                        >  SRR4302534.525399/1‑94 (MQ=60)

                                                        GTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTG                                       >  SRR4302534.3864150/1‑94 (MQ=60)

                                                        GTGAACGGTCGCATGATGCGCAATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTG                                       <  SRR4302534.2436782/94‑1 (MQ=60)

                                                          GAACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTG                                     <  SRR4302534.2043397/94‑1 (MQ=60)

                                                            ACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTT                                   <  SRR4302534.4569785/94‑1 (MQ=60)

                                                            ACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTT                                   >  SRR4302534.6380363/1‑94 (MQ=60)

                                                            ACGGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTT                                   <  SRR4302534.3113366/94‑1 (MQ=60)

                                                              GGTCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGT                                 >  SRR4302534.1412601/1‑94 (MQ=60)

                                                                TCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTAT                               <  SRR4302534.3623350/94‑1 (MQ=60)

                                                                TCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTAT                               <  SRR4302534.5460476/94‑1 (MQ=60)

                                                                TCGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTAT                               >  SRR4302534.2427213/1‑94 (MQ=60)

                                                                 CGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATC                              >  SRR4302534.4767965/1‑94 (MQ=60)

                                                                 CGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATC                              >  SRR4302534.378322/1‑94 (MQ=60)

                                                                 CGCATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATC                              >  SRR4302534.1655791/1‑94 (MQ=60)

                                                                  GCATGATGCGCGAGCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGGGTTGTATCT                             >  SRR4302534.2232057/1‑94 (MQ=60)

                                                                  GCAGGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCT                             <  SRR4302534.3197827/94‑1 (MQ=60)

                                                                    ATGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGG                           >  SRR4302534.3553306/1‑94 (MQ=60)

                                                                     TGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGA                          <  SRR4302534.6150361/94‑1 (MQ=60)

                                                                     TGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGA                          >  SRR4302534.3602212/1‑94 (MQ=60)

                                                                     TGATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGA                          <  SRR4302534.5344747/94‑1 (MQ=60)

                                                                      GATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAG                         <  SRR4302534.6353559/94‑1 (MQ=60)

                                                                      GATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAG                         >  SRR4302534.3239921/1‑94 (MQ=60)

                                                                      GATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAG                         >  SRR4302534.6361916/1‑94 (MQ=60)

                                                                       ATGCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGA                        >  SRR4302534.5599362/1‑94 (MQ=60)

                                                                         GCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATC                      >  SRR4302534.3494935/1‑94 (MQ=60)

                                                                         GCGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATC                      <  SRR4302534.5911189/94‑1 (MQ=60)

                                                                          CGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCG                     <  SRR4302534.2347716/94‑1 (MQ=60)

                                                                          CGCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCG                     <  SRR4302534.2003978/94‑1 (MQ=60)

                                                                           GCGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGA                    >  SRR4302534.1695644/1‑94 (MQ=60)

                                                                            CGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAATACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGAC                   <  SRR4302534.2219336/94‑1 (MQ=60)

                                                                            CGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGAC                   <  SRR4302534.288334/94‑1 (MQ=60)

                                                                            CGATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGAC                   <  SRR4302534.3776696/94‑1 (MQ=60)

                                                                             GATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACC                  <  SRR4302534.611515/94‑1 (MQ=60)

                                                                             GATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACC                  >  SRR4302534.3422505/1‑94 (MQ=60)

                                                                             GATCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACC                  >  SRR4302534.228213/1‑94 (MQ=60)

                                                                               TCGCCTGATCAATCGCGCGATCCTCCAGGCCTGCGAAGACAAACTGTGGGCCGAGCATCAACCGTTATTTGTGTTGaatttggatttcgacccc                >  SRR4302534.2994592/1‑76 (MQ=43)

                                                                               TCGCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGAGTTGTATCTGGAGATCGACCCA                >  SRR4302534.521396/1‑94 (MQ=60)

                                                                                 GCCTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACA              >  SRR4302534.3166896/1‑94 (MQ=60)

                                                                                 GCCTGATCAAGCGCGCGATCCGCCAGGGCTGCGAAGGCAAACTGGGGGCCGATCAGGAACCGGCACTTGTGTTGTATCTGGAGCTCGAACGACA              >  SRR4302534.2473335/1‑94 (MQ=60)

                                                                                   CTGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATC            <  SRR4302534.2598212/94‑1 (MQ=60)

                                                                                    TGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCA           <  SRR4302534.1216655/94‑1 (MQ=60)

                                                                                    TGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCA           >  SRR4302534.5564568/1‑94 (MQ=60)

                                                                                    TGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCA           <  SRR4302534.5401114/94‑1 (MQ=60)

                                                                                    TGATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCA           >  SRR4302534.215361/1‑94 (MQ=60)

                                                                                     GATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAG          >  SRR4302534.6294069/1‑94 (MQ=60)

                                                                                      ATCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGG         >  SRR4302534.6205934/1‑94 (MQ=60)

                                                                                       TCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGT        >  SRR4302534.3801237/1‑94 (MQ=60)

                                                                                       TCAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGT        <  SRR4302534.3431621/94‑1 (MQ=60)

                                                                                        CAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTG       <  SRR4302534.5721405/94‑1 (MQ=60)

                                                                                        CAATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTG       <  SRR4302534.3851096/94‑1 (MQ=60)

                                                                                         AATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGG      >  SRR4302534.773857/1‑94 (MQ=60)

                                                                                         AATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGG      >  SRR4302534.2219630/1‑94 (MQ=60)

                                                                                         AATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGG      >  SRR4302534.2929199/1‑94 (MQ=60)

                                                                                         AATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGG      >  SRR4302534.2915294/1‑94 (MQ=60)

                                                                                          ATCGCGCGATCCGCCAGGCCTGCGCAGACAAACTGGGGGTCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGAGCCACATCtggtgtg     >  SRR4302534.3672243/1‑87 (MQ=60)

                                                                                          ATCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGA     >  SRR4302534.2050750/1‑94 (MQ=60)

                                                                                           TCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGAC    <  SRR4302534.2410305/94‑1 (MQ=60)

                                                                                           TCGCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGAC    <  SRR4302534.3406503/94‑1 (MQ=60)

                                                                                             GCGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGT  >  SRR4302534.786313/1‑94 (MQ=60)

GGGTGGCCGATCCAAATCACACCACGCCCGCACTGGCAGAAATTCAGTATTGCTACGTGAACGGTCGCATGATGCGCGATCGCCTGATCAATCACGCGATCCGCCAGGCCTGCGAAGACAAACTGGGGGCCGATCAGCAACCGGCATTTGTGTTGTATCTGGAGATCGACCCACATCAGGTGGACGT  >  NC_000913/4395705‑4395891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`