Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,476,406 A→G D124G (GAC→GGC)  yjgD → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,476,4060AG99.6% 795.9 / ‑4.6 231D124G (GAC→GGC) yjgDhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (101/129);  total (102/129)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

Only 100 of 247 total aligned reads displayed.

TTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGT  >  NC_000913/4476316‑4476495
                                                                                          |                                                                                         
ttGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGgcggcg                                                                                         <  1:5434677/93‑1 (MQ=255)
 tGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGcggcga                                                                                        <  2:5395811/93‑1 (MQ=255)
 tGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGcggcga                                                                                        <  2:3874258/93‑1 (MQ=255)
   aacaacTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATg                                                                                      >  1:2870176/1‑93 (MQ=255)
    acaacTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGa                                                                                     >  2:3168468/1‑93 (MQ=255)
     caacTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGaa                                                                                    >  2:4317715/1‑93 (MQ=255)
      aacTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGGTGAAg                                                                                   >  2:6124155/1‑93 (MQ=255)
       acTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGTGGCGATGAAGa                                                                                  <  1:3647463/93‑1 (MQ=255)
       acTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGa                                                                                  >  1:3708463/1‑93 (MQ=255)
       acTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGa                                                                                  >  1:6170533/1‑93 (MQ=255)
          gatgaCGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGAttt                                                                               >  2:82743/1‑93 (MQ=255)
           atgaCGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGAtttt                                                                              >  1:6088489/1‑93 (MQ=255)
             gaCGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGt                                                                            >  1:2777559/1‑93 (MQ=255)
              aCGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTc                                                                           <  1:500847/93‑1 (MQ=255)
              aCGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTc                                                                           <  1:6015662/93‑1 (MQ=255)
              aCGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTc                                                                           >  1:1894259/1‑93 (MQ=255)
              aCGCTGACAGAGAAATTTGACGTTGAGTACGACGGATTGGGCACTTTCTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTc                                                                           <  2:952615/93‑1 (MQ=255)
               cGCTGGCAAAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCg                                                                          >  1:6293814/1‑93 (MQ=255)
                gCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGa                                                                         <  2:2428002/93‑1 (MQ=255)
                gCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGa                                                                         <  2:5019342/93‑1 (MQ=255)
                 cTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGACGATCCTAACGGCGAAGATGGCGGGGATGAACATTTTGTCggg                                                                        >  2:4015580/1‑91 (MQ=255)
                 cTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGAt                                                                        <  2:4079813/93‑1 (MQ=255)
                 cTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGAt                                                                        <  1:6007011/93‑1 (MQ=255)
                     cAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAg                                                                    >  2:5507034/1‑93 (MQ=255)
                      agagAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGa                                                                   >  2:2723369/1‑93 (MQ=255)
                      agagAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGa                                                                   >  1:5559045/1‑93 (MQ=255)
                      agagAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGa                                                                   <  1:1142216/93‑1 (MQ=255)
                       gagaAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGAc                                                                  >  2:5581338/1‑93 (MQ=255)
                        agaAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACg                                                                 >  2:5932748/1‑93 (MQ=255)
                         gaAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGa                                                                >  2:3657028/1‑93 (MQ=255)
                         gaAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGa                                                                <  1:4911029/93‑1 (MQ=255)
                          aaaTTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGAt                                                               <  1:4727189/93‑1 (MQ=255)
                          aaaTTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGAt                                                               <  1:560318/93‑1 (MQ=255)
                           aaTTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATg                                                              <  2:1897165/93‑1 (MQ=255)
                           aaTTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCAAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATg                                                              <  1:4532411/93‑1 (MQ=255)
                            aTTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGa                                                             <  1:2210236/93‑1 (MQ=255)
                            aTTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGa                                                             <  1:3033234/93‑1 (MQ=255)
                            aTTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGa                                                             <  1:660315/93‑1 (MQ=255)
                             tttGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGAc                                                            <  2:3800325/93‑1 (MQ=255)
                               tGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACgg                                                          <  1:4263155/93‑1 (MQ=255)
                               tGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACgg                                                          >  2:3748562/1‑93 (MQ=255)
                                gACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGa                                                         >  2:3097698/1‑93 (MQ=255)
                                gACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGa                                                         >  2:5720988/1‑93 (MQ=255)
                                   gTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGtt                                                      >  2:3177559/1‑93 (MQ=255)
                                   gTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGtt                                                      <  2:1636798/93‑1 (MQ=255)
                                    ttGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTc                                                     <  1:3588859/93‑1 (MQ=255)
                                     tGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCg                                                    <  2:5247661/93‑1 (MQ=255)
                                         tACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCAc                                                <  2:2219879/93‑1 (MQ=255)
                                           cgacgGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTa                                              <  2:2785126/93‑1 (MQ=255)
                                            gacgGATGGGCCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTaa                                             <  1:5489065/93‑1 (MQ=255)
                                                gATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTaa                                         >  2:5799676/1‑93 (MQ=255)
                                                gATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTaa                                         <  2:473886/93‑1 (MQ=255)
                                                gATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTaa                                         >  1:3802380/1‑93 (MQ=255)
                                                 aTGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAg                                        <  1:4821822/93‑1 (MQ=255)
                                                 aTGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAg                                        <  1:1249823/93‑1 (MQ=255)
                                                 aTGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAg                                        <  1:411411/93‑1 (MQ=255)
                                                  tGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGc                                       <  1:1743979/93‑1 (MQ=255)
                                                   ggggCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCg                                      >  1:2661140/1‑93 (MQ=255)
                                                    gggCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGt                                     <  2:6439133/93‑1 (MQ=255)
                                                      gCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAg                                   <  1:1693474/93‑1 (MQ=255)
                                                       cACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAgc                                  <  2:1259531/93‑1 (MQ=255)
                                                       cACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAgc                                  <  1:6126948/93‑1 (MQ=255)
                                                        acttacttTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAgcg                                 >  1:4497440/1‑93 (MQ=255)
                                                         cttacttTGAAGATCCTAACGGCGAAGATGGCGGCGGTGAAGATTTTGTCGATGAAGACGACGACGGAGATCGCCACTAAATAAGCGTAgcgc                                >  1:3283976/1‑93 (MQ=255)
                                                         cttacttTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAgcgc                                >  1:5863811/1‑93 (MQ=255)
                                                            acttTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATc                             <  2:301895/93‑1 (MQ=255)
                                                            acttTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATc                             <  1:2940080/93‑1 (MQ=255)
                                                             cttTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCa                            <  1:888402/93‑1 (MQ=255)
                                                              tttGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAg                           >  2:4620682/1‑93 (MQ=255)
                                                              tttGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAg                           <  2:2106290/93‑1 (MQ=255)
                                                               ttGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAgg                          <  2:3201726/93‑1 (MQ=255)
                                                                  aaGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGccc                       <  2:5902716/93‑1 (MQ=255)
                                                                  aaGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGccc                       <  1:1953220/93‑1 (MQ=255)
                                                                    gATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAg                     >  2:5155101/1‑93 (MQ=255)
                                                                    gATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAg                     >  1:3186712/1‑93 (MQ=255)
                                                                    gATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAg                     <  2:1669566/93‑1 (MQ=255)
                                                                     aTCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAgg                    >  2:4779314/1‑93 (MQ=255)
                                                                     aTCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAgg                    >  2:6007192/1‑93 (MQ=255)
                                                                     aTCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAgg                    >  1:1096919/1‑93 (MQ=255)
                                                                     aTCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAgg                    >  2:2333887/1‑93 (MQ=255)
                                                                      tCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGt                   >  2:5752185/1‑93 (MQ=255)
                                                                      tCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGt                   >  1:5872060/1‑93 (MQ=255)
                                                                       ccTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTa                  >  2:2688587/1‑93 (MQ=255)
                                                                       ccTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTa                  <  1:642382/93‑1 (MQ=255)
                                                                        cTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAg                 >  2:454109/1‑93 (MQ=255)
                                                                           aCGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTc              >  2:2825816/1‑93 (MQ=255)
                                                                           aCGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTc              >  2:6478432/1‑93 (MQ=255)
                                                                           aCGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTc              <  2:122867/93‑1 (MQ=255)
                                                                           aCGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTc              >  2:5095004/1‑93 (MQ=255)
                                                                            cGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTcc             <  1:2102746/93‑1 (MQ=255)
                                                                            cGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTcc             >  1:5892948/1‑93 (MQ=255)
                                                                            cGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTcc             >  1:136574/1‑93 (MQ=255)
                                                                              gCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCGGGCCCAGGTAGTTCCTg           >  2:630179/1‑93 (MQ=255)
                                                                              gCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTg           >  2:1036073/1‑93 (MQ=255)
                                                                                  aGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAAc       >  1:3982678/1‑93 (MQ=255)
                                                                                    aTGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCt     <  1:3441799/93‑1 (MQ=255)
                                                                                     tGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTg    >  2:109638/1‑93 (MQ=255)
                                                                                     tGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTg    <  2:1779785/93‑1 (MQ=255)
                                                                                     tGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTg    <  2:4300785/93‑1 (MQ=255)
                                                                                       gcggcgATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGt  <  1:1422586/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGT  >  NC_000913/4476316‑4476495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGTTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAAT  >  NC_000913/4476313‑4476499
                                                                                             |                                                                                             
AGGTTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGGTGGCGG                                                                                               >  SRR4302534.2340259/1‑94 (MQ=60)
AGGTTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGG                                                                                               >  SRR4302534.1127705/1‑94 (MQ=60)
AGGTTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGG                                                                                               >  SRR4302534.4968889/1‑94 (MQ=60)
   TTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGA                                                                                            <  SRR4302534.5581018/94‑1 (MQ=60)
     GAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATG                                                                                          >  SRR4302534.2945540/1‑94 (MQ=60)
       ACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAA                                                                                        <  SRR4302534.4128189/94‑1 (MQ=60)
         AACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGA                                                                                      >  SRR4302534.6138372/1‑94 (MQ=60)
         AACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGA                                                                                      >  SRR4302534.6338379/1‑94 (MQ=60)
         AACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGA                                                                                      >  SRR4302534.3808157/1‑94 (MQ=60)
          ACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGTGGCGATGAAGAT                                                                                     <  SRR4302534.3745469/94‑1 (MQ=60)
          ACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGAT                                                                                     <  SRR4302534.3731019/94‑1 (MQ=60)
            TGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTT                                                                                   >  SRR4302534.1908717/1‑94 (MQ=60)
             GATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTT                                                                                  >  SRR4302534.6254057/1‑94 (MQ=60)
             GATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTT                                                                                  <  SRR4302534.6216356/94‑1 (MQ=60)
             GATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTT                                                                                  <  SRR4302534.3182379/94‑1 (MQ=60)
               TGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGT                                                                                >  SRR4302534.2850628/1‑94 (MQ=60)
               TGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGT                                                                                <  SRR4302534.3680102/94‑1 (MQ=60)
                GACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTC                                                                               >  SRR4302534.1943675/1‑94 (MQ=60)
                 ACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCG                                                                              <  SRR4302534.6179123/94‑1 (MQ=60)
                 ACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCG                                                                              <  SRR4302534.513322/94‑1 (MQ=60)
                 ACGCTGGCAAAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCG                                                                              >  SRR4302534.6464067/1‑94 (MQ=60)
                    CTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATG                                                                           <  SRR4302534.6251972/94‑1 (MQ=60)
                    CTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATA                                                                           <  SRR4302534.6170208/94‑1 (MQ=60)
                      GGCAGAGAAATTTGACGTTGAGTACGACGGGTGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGGAGAATTTGTCGATGAA                                                                         >  SRR4302534.384549/1‑94 (MQ=60)
                        CAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGA                                                                       >  SRR4302534.5709131/1‑94 (MQ=60)
                         AGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGAC                                                                      >  SRR4302534.6115424/1‑94 (MQ=60)
                         AGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGAC                                                                      <  SRR4302534.1172121/94‑1 (MQ=60)
                          GAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACG                                                                     <  SRR4302534.1399244/94‑1 (MQ=60)
                          GAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACG                                                                     >  SRR4302534.4623265/1‑94 (MQ=60)
                           AGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGA                                                                    >  SRR4302534.3839436/1‑94 (MQ=60)
                            GAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGAT                                                                   <  SRR4302534.5044129/94‑1 (MQ=60)
                             AAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATG                                                                  >  SRR4302534.5702633/1‑94 (MQ=60)
                             AAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATG                                                                  <  SRR4302534.574114/94‑1 (MQ=60)
                             AAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATG                                                                  <  SRR4302534.4855565/94‑1 (MQ=60)
                              AATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCAAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGA                                                                 <  SRR4302534.4655234/94‑1 (MQ=60)
                               ATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGAC                                                                <  SRR4302534.676369/94‑1 (MQ=60)
                               ATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGAC                                                                <  SRR4302534.2476913/94‑1 (MQ=60)
                               ATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGAC                                                                <  SRR4302534.5172087/94‑1 (MQ=60)
                               ATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGAC                                                                <  SRR4302534.2268767/94‑1 (MQ=60)
                               ATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGAC                                                                <  SRR4302534.3112777/94‑1 (MQ=60)
                                TTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACG                                                               <  SRR4302534.5282255/94‑1 (MQ=60)
                                TTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACG                                                               >  SRR4302534.2206111/1‑94 (MQ=60)
                                  TGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGA                                                             <  SRR4302534.4378296/94‑1 (MQ=60)
                                  TGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGA                                                             >  SRR4302534.1949972/1‑94 (MQ=60)
                                  TGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGA                                                             <  SRR4302534.3766457/94‑1 (MQ=60)
                                   GACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAG                                                            <  SRR4302534.1299467/94‑1 (MQ=60)
                                   GACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGAAGATGACGGAG                                                            >  SRR4302534.6293649/1‑94 (MQ=60)
                                    ACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGT                                                           <  SRR4302534.4194480/94‑1 (MQ=60)
                                     CGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTT                                                          <  SRR4302534.4937590/94‑1 (MQ=60)
                                      GTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTC                                                         <  SRR4302534.3057611/94‑1 (MQ=60)
                                      GTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCAAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTC                                                         <  SRR4302534.2823479/94‑1 (MQ=60)
                                       TTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCG                                                        <  SRR4302534.3685380/94‑1 (MQ=60)
                                         GAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCC                                                      <  SRR4302534.6029736/94‑1 (MQ=60)
                                         GAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCC                                                      <  SRR4302534.1723212/94‑1 (MQ=60)
                                          AGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCA                                                     >  SRR4302534.4337469/1‑94 (MQ=60)
                                           GTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCAC                                                    >  SRR4302534.3089478/1‑94 (MQ=60)
                                             ACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTA                                                  <  SRR4302534.5737319/94‑1 (MQ=60)
                                              CGACGGATGGGGCACTTACCTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAA                                                 >  SRR4302534.4550227/1‑94 (MQ=60)
                                               GACGGATGGGCCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAAT                                                <  SRR4302534.5637102/94‑1 (MQ=60)
                                                  GGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAA                                             >  SRR4302534.3904470/1‑94 (MQ=60)
                                                   GATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAG                                            <  SRR4302534.2651870/94‑1 (MQ=60)
                                                    ATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGC                                           <  SRR4302534.887736/94‑1 (MQ=60)
                                                    ATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGC                                           <  SRR4302534.1282761/94‑1 (MQ=60)
                                                    ATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGC                                           <  SRR4302534.421929/94‑1 (MQ=60)
                                                    ATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGC                                           <  SRR4302534.4952682/94‑1 (MQ=60)
                                                     TGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCG                                          <  SRR4302534.6422261/94‑1 (MQ=60)
                                                     TGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCG                                          >  SRR4302534.2731492/1‑94 (MQ=60)
                                                     TGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCG                                          <  SRR4302534.1789244/94‑1 (MQ=60)
                                                      GGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGT                                         >  SRR4302534.6566210/1‑94 (MQ=60)
                                                       GGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTA                                        <  SRR4302534.888189/94‑1 (MQ=60)
                                                       GGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTA                                        <  SRR4302534.1456495/94‑1 (MQ=60)
                                                        GGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAG                                       <  SRR4302534.4607978/94‑1 (MQ=60)
                                                         GCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGC                                      <  SRR4302534.1737484/94‑1 (MQ=60)
                                                         GCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGC                                      >  SRR4302534.5950244/1‑94 (MQ=60)
                                                          TACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCG                                     >  SRR4302534.4619155/1‑94 (MQ=60)
                                                          CACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCG                                     <  SRR4302534.214556/94‑1 (MQ=60)
                                                          CACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCG                                     >  SRR4302534.3522090/1‑94 (MQ=60)
                                                          CACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCG                                     <  SRR4302534.5747750/94‑1 (MQ=60)
                                                          CACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCG                                     <  SRR4302534.6293600/94‑1 (MQ=60)
                                                           ACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGGTGAAGATTTTGTCGATGAAGACGACGACGGAGATCGCCACTAAATAAGCGTAGCGC                                    >  SRR4302534.3371509/1‑94 (MQ=60)
                                                           ACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGC                                    >  SRR4302534.6023022/1‑94 (MQ=60)
                                                           ACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGC                                    >  SRR4302534.3284936/1‑94 (MQ=60)
                                                           ACTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGC                                    <  SRR4302534.4688275/94‑1 (MQ=60)
                                                            CTTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCA                                   >  SRR4302534.1535523/1‑94 (MQ=60)
                                                             TTACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCAT                                  <  SRR4302534.2887874/94‑1 (MQ=60)
                                                               ACTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCA                                <  SRR4302534.3017234/94‑1 (MQ=60)
                                                                CTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGCTGAAGACGATGACGGAGTTCGCCACTAATTAAGCGCAGCGCATCAG                               >  SRR4302534.2123050/1‑94 (MQ=60)
                                                                CTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAG                               >  SRR4302534.1831515/1‑94 (MQ=60)
                                                                CTTTGAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAG                               <  SRR4302534.910896/94‑1 (MQ=60)
                                                                    GAAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCC                           <  SRR4302534.1127761/94‑1 (MQ=60)
                                                                    GAAGATACTAACGACGAATATGGCGGCGATGATTACTTGGTCGATGAATACGATGACGGAATTCGCCACTAATTAAGCGTAGCGCATCTGGCCC                           <  SRR4302534.2196445/94‑1 (MQ=34)
                                                                     AAGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCA                          <  SRR4302534.2004337/94‑1 (MQ=60)
                                                                      AGATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAG                         >  SRR4302534.3271023/1‑94 (MQ=60)
                                                                       GATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGG                        >  SRR4302534.1125475/1‑94 (MQ=60)
                                                                       GATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGG                        >  SRR4302534.4013102/1‑94 (MQ=60)
                                                                        ATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGGCCAGGT                       >  SRR4302534.2874474/1‑94 (MQ=60)
                                                                        ATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGT                       >  SRR4302534.5037571/1‑94 (MQ=60)
                                                                        ATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGT                       <  SRR4302534.466640/94‑1 (MQ=60)
                                                                        ATCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGT                       >  SRR4302534.6031488/1‑94 (MQ=60)
                                                                         TCCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTA                      <  SRR4302534.6629176/94‑1 (MQ=60)
                                                                          CCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAG                     >  SRR4302534.6288557/1‑94 (MQ=60)
                                                                          CCTAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAG                     <  SRR4302534.657974/94‑1 (MQ=60)
                                                                            TAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTT                   <  SRR4302534.1234906/94‑1 (MQ=60)
                                                                            TAACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTT                   >  SRR4302534.3489816/1‑94 (MQ=60)
                                                                             AACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGACGACGATTTCGGAGTTCGCCACTAATTAAGCGCAGCGAATCAGGCCCATGTAGCTC                  >  SRR4302534.2959019/1‑94 (MQ=60)
                                                                             AACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTC                  >  SRR4302534.1783074/1‑94 (MQ=60)
                                                                              ACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCC                 >  SRR4302534.140145/1‑94 (MQ=60)
                                                                              ACGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCC                 >  SRR4302534.6052957/1‑94 (MQ=60)
                                                                               CGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCT                <  SRR4302534.2157942/94‑1 (MQ=60)
                                                                               CGGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCT                <  SRR4302534.600930/94‑1 (MQ=60)
                                                                                GGCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTG               >  SRR4302534.3735577/1‑94 (MQ=60)
                                                                                 GCGAAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGC              >  SRR4302534.6305137/1‑94 (MQ=60)
                                                                                    AAGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAAC           >  SRR4302534.4089258/1‑94 (MQ=60)
                                                                                     AGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACC          <  SRR4302534.6171890/94‑1 (MQ=60)
                                                                                     AGATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACC          >  SRR4302534.5117056/1‑94 (MQ=60)
                                                                                       ATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTG        <  SRR4302534.4991246/94‑1 (MQ=60)
                                                                                       ATGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTG        <  SRR4302534.3534303/94‑1 (MQ=60)
                                                                                        TGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGG       <  SRR4302534.6549940/94‑1 (MQ=60)
                                                                                        TGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGG       <  SRR4302534.6032498/94‑1 (MQ=60)
                                                                                        TGGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGG       >  SRR4302534.5612175/1‑94 (MQ=60)
                                                                                         GGCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGT      >  SRR4302534.3001667/1‑94 (MQ=60)
                                                                                          GCGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTA     <  SRR4302534.1460020/94‑1 (MQ=60)
                                                                                           CGGCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGA    >  SRR4302534.4834964/1‑94 (MQ=60)
                                                                                             GCGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAAT  <  SRR4302534.3607952/94‑1 (MQ=60)
                                                                                             |                                                                                             
AGGTTGAACAACTGATGACGCTGGCAGAGAAATTTGACGTTGAGTACGACGGATGGGGCACTTACTTTGAAGATCCTAACGGCGAAGATGGCGACGATGAAGATTTTGTCGATGAAGACGATGACGGAGTTCGCCACTAATTAAGCGTAGCGCATCAGGCCCAGGTAGTTCCTGCAACCTGGTGAAT  >  NC_000913/4476313‑4476499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑