Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	884	257.2	12958910	96.9%	12557183	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,542,425	A→G	Y168C (TAC→TGC)	b4316 →	Pil

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,542,425	0	A	G	99.5%	741.2 / ‑4.7	211	Y168C (TAC→TGC)	b4316	Pil
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (101/109); total (101/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01

Only 100 of 215 total aligned reads displayed.

AATTAGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTT  >  NC_000913/4542335‑4542514

aaTTAGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGcc                                                                                         >  1:4029895/1‑93 (MQ=255)

 aTTAGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCt                                                                                        <  2:1981683/93‑1 (MQ=255)

  ttAGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTg                                                                                       <  1:1364772/93‑1 (MQ=255)

      cGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACgg                                                                                   >  2:898955/1‑93 (MQ=255)

      cGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACgg                                                                                   <  2:1433647/93‑1 (MQ=255)

       gTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGt                                                                                  >  1:1988785/1‑93 (MQ=255)

        ttGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTa                                                                                 >  1:3666874/1‑93 (MQ=255)

          gCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTacc                                                                               >  2:6065244/1‑91 (MQ=255)

           ccACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGTCGGTAACa                                                                              <  1:6295989/93‑1 (MQ=255)

           ccACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACa                                                                              >  1:6054672/1‑93 (MQ=255)

            cACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACag                                                                             <  1:1091414/93‑1 (MQ=255)

            cACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACag                                                                             >  2:4060236/1‑93 (MQ=255)

            cACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACag                                                                             >  2:3764224/1‑93 (MQ=255)

             aCCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACACTGATTAACCCGACACCCTATTGCCTGACGGTAACaga                                                                            >  1:1732598/1‑93 (MQ=255)

              cccGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACagag                                                                           <  2:5243375/93‑1 (MQ=255)

               ccGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGt                                                                          <  1:5054073/93‑1 (MQ=255)

               ccGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGt                                                                          >  2:2189910/1‑93 (MQ=255)

               ccGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGt                                                                          <  2:1886914/93‑1 (MQ=255)

                cGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACCCCCTATTGCCTGACGGTAACAGAGtt                                                                         <  2:2551068/93‑1 (MQ=255)

                cGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGtt                                                                         <  2:3028051/93‑1 (MQ=255)

                cGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGtt                                                                         <  1:6063723/93‑1 (MQ=255)

                cGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGtt                                                                         >  2:2608419/1‑93 (MQ=255)

                 gATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTg                                                                        >  1:3314176/1‑93 (MQ=255)

                  aTCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGa                                                                       >  1:891080/1‑93 (MQ=255)

                  aTCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGa                                                                       >  2:4205284/1‑93 (MQ=255)

                  aTCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGATGa                                                                       >  2:6467555/1‑93 (MQ=255)

                   tCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGCATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACCGAGTTgca                                                                      >  1:4373885/1‑91 (MQ=255)

                   tCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGaa                                                                      <  2:5497707/93‑1 (MQ=255)

                     aGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATg                                                                    >  2:2319762/1‑93 (MQ=255)

                      ggCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACACGACACCCTATTGCCTGACGGTAACAGAGTTGAATGc                                                                   >  2:3403984/1‑93 (MQ=255)

                         cGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCgg                                                                >  2:3586812/1‑93 (MQ=255)

                         cGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCgg                                                                <  1:4648547/93‑1 (MQ=255)

                          gCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGa                                                               >  2:6408468/1‑93 (MQ=255)

                           cAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGaa                                                              >  2:4917447/1‑93 (MQ=255)

                            aGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAAc                                                             >  1:1072427/1‑93 (MQ=255)

                            aGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAAc                                                             >  2:4721318/1‑93 (MQ=255)

                            aGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAAc                                                             <  1:233385/93‑1 (MQ=255)

                              aaaaaTTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAAccc                                                           >  2:4814006/1‑93 (MQ=255)

                              aaaaaTTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAAccc                                                           >  2:1676109/1‑93 (MQ=255)

                                 aaTTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCggg                                                        <  2:1546940/93‑1 (MQ=255)

                                  aTTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGt                                                       <  1:267080/93‑1 (MQ=255)

                                  aTTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGt                                                       <  2:2208391/93‑1 (MQ=255)

                                   ttAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGtt                                                      >  1:473624/1‑93 (MQ=255)

                                     aaGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCt                                                    <  1:5347134/93‑1 (MQ=255)

                                     aaGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCt                                                    >  2:764497/1‑93 (MQ=255)

                                      aGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCtt                                                   <  1:2959443/93‑1 (MQ=255)

                                        aTTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGa                                                 >  1:3171987/1‑93 (MQ=255)

                                         tttCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGaa                                                >  1:453307/1‑93 (MQ=255)

                                           tcgtcgTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGaaaa                                              <  2:4366504/93‑1 (MQ=255)

                                               cgtAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCa                                          >  2:1797869/1‑93 (MQ=255)

                                                gtAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCAt                                         >  1:3945380/1‑93 (MQ=255)

                                                gtAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCAt                                         <  2:556229/93‑1 (MQ=255)

                                                  aGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAAACCGGGTTCTTGAAAATGCATTg                                       >  1:1607099/1‑93 (MQ=255)

                                                   gcgcGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTgg                                      >  1:3636124/1‑93 (MQ=255)

                                                   gcgcGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTgg                                      >  2:4411149/1‑93 (MQ=255)

                                                     gcgAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTg                                    >  2:5175658/1‑93 (MQ=255)

                                                       gAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGTTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGcc                                  <  1:3846257/93‑1 (MQ=255)

                                                       gAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGcc                                  <  1:244275/93‑1 (MQ=255)

                                                       gAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGcc                                  <  2:1291536/93‑1 (MQ=255)

                                                       gAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGcc                                  <  1:4121213/93‑1 (MQ=255)

                                                       gAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGcc                                  >  1:4902108/1‑93 (MQ=255)

                                                        aaTTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCt                                 <  2:1585173/93‑1 (MQ=255)

                                                        aaTTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCt                                 <  2:6128633/93‑1 (MQ=255)

                                                        aaTTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCt                                 >  2:5047759/1‑93 (MQ=255)

                                                         aTTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTc                                <  2:3261884/93‑1 (MQ=255)

                                                          ttCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTcc                               <  1:6150701/93‑1 (MQ=255)

                                                           tctcTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCa                              <  1:5883445/93‑1 (MQ=255)

                                                             tctGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAAt                            <  2:2500468/93‑1 (MQ=255)

                                                             tctGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAAt                            <  1:4243306/93‑1 (MQ=255)

                                                              ctGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATg                           >  1:506410/1‑93 (MQ=255)

                                                               tGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATgg                          >  2:5110419/1‑93 (MQ=255)

                                                               tGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATgg                          <  1:5991439/93‑1 (MQ=255)

                                                                gACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATggg                         >  1:6461439/1‑93 (MQ=255)

                                                                gACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATggg                         <  1:3100243/93‑1 (MQ=255)

                                                                 aCGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGGTCTTGAAAATGCATTGGTGCCTCCAATGGGc                        >  2:4005620/1‑93 (MQ=255)

                                                                  cGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCg                       >  2:294374/1‑93 (MQ=255)

                                                                    cTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGaa                     >  1:666566/1‑93 (MQ=255)

                                                                    cTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGaa                     <  2:4581850/93‑1 (MQ=255)

                                                                     tGATTAACCCGTCACCGTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGaaa                    <  1:2277271/93‑1 (MQ=255)

                                                                       aTTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGGAAGc                  >  2:122380/1‑93 (MQ=255)

                                                                       aTTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGc                  <  1:3554917/93‑1 (MQ=255)

                                                                        ttAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCa                 <  1:560994/93‑1 (MQ=255)

                                                                         tAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCAc                >  1:5425607/1‑93 (MQ=255)

                                                                           aCCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACgg              >  1:225975/1‑93 (MQ=255)

                                                                            cccGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGt             <  1:388493/93‑1 (MQ=255)

                                                                              cGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTa           >  1:2923780/1‑93 (MQ=255)

                                                                              cGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTa           >  2:2686905/1‑93 (MQ=255)

                                                                                acacCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTaaa         <  2:5838656/93‑1 (MQ=255)

                                                                                 cgcccTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAAt        <  2:4787381/91‑1 (MQ=255)

                                                                                 cacCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAAt        >  1:5821748/1‑93 (MQ=255)

                                                                                 cacCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAAt        <  1:1478389/93‑1 (MQ=255)

                                                                                 cacCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAAt        <  1:6415415/93‑1 (MQ=255)

                                                                                 cacCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAAt        <  1:1236233/93‑1 (MQ=255)

                                                                                 cacCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAAt        <  1:2190052/93‑1 (MQ=255)

                                                                                  acCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAAtt       >  2:3445678/1‑93 (MQ=255)

                                                                                   cccTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTg      >  1:3371810/1‑93 (MQ=255)

                                                                                     cTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGcc    <  2:3279387/93‑1 (MQ=255)

                                                                                      tATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCt   <  1:4865318/93‑1 (MQ=255)

                                                                                      tATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCt   <  1:447083/93‑1 (MQ=255)

                                                                                       aTTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCtt  <  2:535506/93‑1 (MQ=255)

AATTAGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTT  >  NC_000913/4542335‑4542514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTAAATTAGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGA  >  NC_000913/4542332‑4542518

CTAAATTAGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTG                                                                                               >  SRR4302534.2671590/1‑94 (MQ=60)

  AAATTAGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCC                                                                                             >  SRR4302534.4137939/1‑94 (MQ=60)

   AATTAGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCT                                                                                            >  SRR4302534.4785163/1‑94 (MQ=60)

    ATTAGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTG                                                                                           <  SRR4302534.298754/94‑1 (MQ=60)

     TTAGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGA                                                                                          <  SRR4302534.1400721/94‑1 (MQ=60)

     TTAGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGA                                                                                          <  SRR4302534.413806/94‑1 (MQ=60)

       AGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACG                                                                                        <  SRR4302534.6629565/94‑1 (MQ=60)

         CGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGT                                                                                      >  SRR4302534.2040863/1‑94 (MQ=60)

          GTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTA                                                                                     >  SRR4302534.3765429/1‑94 (MQ=60)

          GTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTA                                                                                     >  SRR4302534.2166037/1‑94 (MQ=60)

             GCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTATCCCGACCCCCTATTGCCTGTCGGTAACA                                                                                  <  SRR4302534.3000847/94‑1 (MQ=60)

             GCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACA                                                                                  >  SRR4302534.6219319/1‑94 (MQ=60)

             GCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACA                                                                                  >  SRR4302534.6219318/1‑94 (MQ=60)

              CCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGTCGGTAACAA                                                                                 <  SRR4302534.6466285/94‑1 (MQ=60)

               CACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGA                                                                                <  SRR4302534.1119807/94‑1 (MQ=60)

               CACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACACTGATTAACCCGACACCCTATTGCCTGACGGTAACAGA                                                                                >  SRR4302534.1777601/1‑94 (MQ=60)

                 CCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGT                                                                              <  SRR4302534.2793938/94‑1 (MQ=60)

                  CCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTT                                                                             <  SRR4302534.5190578/94‑1 (MQ=60)

                   CGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACTCGACACCCTATTGCCTCACGGTAACAGAGCTG                                                                            >  SRR4302534.5570463/1‑94 (MQ=60)

                   CGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTG                                                                            >  SRR4302534.2454157/1‑94 (MQ=60)

                   CGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTG                                                                            >  SRR4302534.5852148/1‑94 (MQ=60)

                   CGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTG                                                                            <  SRR4302534.6228593/94‑1 (MQ=60)

                   CGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTG                                                                            >  SRR4302534.3402722/1‑94 (MQ=60)

                    GATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGA                                                                           >  SRR4302534.913646/1‑94 (MQ=60)

                    GATCAGGCCGCAGAAAAATTAAGATTGCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGA                                                                           <  SRR4302534.4217002/94‑1 (MQ=60)

                     ATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGCATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACCGAGTTGCA                                                                          >  SRR4302534.4491771/1‑94 (MQ=60)

                     ATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAA                                                                          <  SRR4302534.2691138/94‑1 (MQ=60)

                      TCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAAT                                                                         >  SRR4302534.5010214/1‑94 (MQ=60)

                       CAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGCCGGTAACAGAGTTGAATG                                                                        >  SRR4302534.1117479/1‑94 (MQ=60)

                       CAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATG                                                                        <  SRR4302534.4094317/94‑1 (MQ=60)

                       CAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATG                                                                        <  SRR4302534.6227917/94‑1 (MQ=60)

                        AGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGC                                                                       <  SRR4302534.6249980/94‑1 (MQ=60)

                         GGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCC                                                                      <  SRR4302534.1261085/94‑1 (MQ=60)

                          GCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGGCACCCTATTGCCTGACGGTAACAGAGTTGAATGCCG                                                                     <  SRR4302534.4936547/94‑1 (MQ=60)

                          GCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCG                                                                     <  SRR4302534.5733168/94‑1 (MQ=60)

                           CCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGG                                                                    >  SRR4302534.604956/1‑94 (MQ=60)

                           CCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGG                                                                    <  SRR4302534.1497480/94‑1 (MQ=60)

                            CGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGA                                                                   <  SRR4302534.4774740/94‑1 (MQ=60)

                              CAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAAC                                                                 >  SRR4302534.3936725/1‑94 (MQ=60)

                              CAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAAC                                                                 >  SRR4302534.1100252/1‑94 (MQ=60)

                               AGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACC                                                                <  SRR4302534.5456879/94‑1 (MQ=60)

                               AGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACC                                                                <  SRR4302534.3652759/94‑1 (MQ=60)

                               AGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACC                                                                >  SRR4302534.3761783/1‑94 (MQ=60)

                               AGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACC                                                                <  SRR4302534.239492/94‑1 (MQ=60)

                                  AAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGG                                                             <  SRR4302534.4886934/94‑1 (MQ=60)

                                    AATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGT                                                           <  SRR4302534.1927350/94‑1 (MQ=60)

                                    AATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGT                                                           >  SRR4302534.662242/1‑94 (MQ=60)

                                     ATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTT                                                          <  SRR4302534.274006/94‑1 (MQ=60)

                                     ATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTT                                                          >  SRR4302534.485539/1‑94 (MQ=60)

                                     ATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTT                                                          <  SRR4302534.1317425/94‑1 (MQ=60)

                                     ATTAAGATTACGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTT                                                          >  SRR4302534.6480715/1‑94 (MQ=60)

                                       TAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCT                                                        >  SRR4302534.2330701/1‑94 (MQ=60)

                                       TAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCT                                                        >  SRR4302534.4400567/1‑94 (MQ=60)

                                        AAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTT                                                       <  SRR4302534.5491305/94‑1 (MQ=60)

                                         AGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTG                                                      <  SRR4302534.3036989/94‑1 (MQ=60)

                                          GATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGA                                                     >  SRR4302534.3255690/1‑94 (MQ=60)

                                           ATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAA                                                    >  SRR4302534.464790/1‑94 (MQ=60)

                                                GTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGC                                               <  SRR4302534.4700219/94‑1 (MQ=60)

                                                 TCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCA                                              <  SRR4302534.2255164/94‑1 (MQ=60)

                                                 GCGTAGCGCGAATTCTCTGACGCTGATTACCCCGACACCCTATTGCCTGACGGAAACAGAGTTGAATGCCGGACCCCGGTTTCTAAAAAATGCA                                              <  SRR4302534.1315957/94‑1 (MQ=60)

                                                  CGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCAT                                             >  SRR4302534.4051022/1‑94 (MQ=60)

                                                   GTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATT                                            <  SRR4302534.3112000/94‑1 (MQ=60)

                                                    TAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAAACCGGGTTCTTGAAAATGCATTG                                           >  SRR4302534.1649071/1‑94 (MQ=60)

                                                     AGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGG                                          >  SRR4302534.3733861/1‑94 (MQ=60)

                                                       CGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTG                                        <  SRR4302534.3435594/94‑1 (MQ=60)

                                                         CGAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCC                                      >  SRR4302534.5035013/1‑94 (MQ=60)

                                                          GAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGTTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCT                                     <  SRR4302534.3949545/94‑1 (MQ=60)

                                                          GAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCT                                     >  SRR4302534.517299/1‑94 (MQ=60)

                                                          GAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCT                                     <  SRR4302534.4232116/94‑1 (MQ=60)

                                                          GAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCT                                     <  SRR4302534.250666/94‑1 (MQ=60)

                                                          GAATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCT                                     <  SRR4302534.2370235/94‑1 (MQ=60)

                                                           AATTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTC                                    >  SRR4302534.1662587/1‑94 (MQ=60)

                                                             TTCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCA                                  <  SRR4302534.6318029/94‑1 (MQ=60)

                                                              TCTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAA                                 <  SRR4302534.6043193/94‑1 (MQ=60)

                                                               CTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAAT                                <  SRR4302534.4002255/94‑1 (MQ=60)

                                                               CTCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAAT                                <  SRR4302534.63491/94‑1 (MQ=60)

                                                                TCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATG                               <  SRR4302534.4357741/94‑1 (MQ=60)

                                                                TCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATG                               >  SRR4302534.3599579/1‑94 (MQ=60)

                                                                TCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATG                               <  SRR4302534.1590925/94‑1 (MQ=60)

                                                                TCTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATG                               >  SRR4302534.519001/1‑94 (MQ=60)

                                                                 CTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGG                              <  SRR4302534.3982886/94‑1 (MQ=60)

                                                                 CTGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGG                              <  SRR4302534.5191034/94‑1 (MQ=60)

                                                                  TGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGG                             <  SRR4302534.6154202/94‑1 (MQ=60)

                                                                  TGACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGG                             >  SRR4302534.6635480/1‑94 (MQ=60)

                                                                   GACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGC                            <  SRR4302534.3181795/94‑1 (MQ=60)

                                                                    ACGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCG                           >  SRR4302534.3382503/1‑94 (MQ=60)

                                                                     CGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGA                          >  SRR4302534.3247539/1‑94 (MQ=60)

                                                                     CGCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGA                          >  SRR4302534.5759635/1‑94 (MQ=60)

                                                                      GCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAA                         <  SRR4302534.4528007/94‑1 (MQ=60)

                                                                      GCTGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAA                         >  SRR4302534.682790/1‑94 (MQ=60)

                                                                        TGTTTACCCCGACACCCTATTGCCGGAGGGTAACAGAGTTGAATGCCGGAACGGGGGTTCTAAAAAATGCATTGGTGCCTCCAATGGGCGAAAG                       <  SRR4302534.1904267/94‑1 (MQ=60)

                                                                        TGATTAACCCGTCACCGTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAG                       <  SRR4302534.2337704/94‑1 (MQ=60)

                                                                        TGATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAG                       >  SRR4302534.4337983/1‑94 (MQ=60)

                                                                         GATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGC                      >  SRR4302534.4651084/1‑94 (MQ=60)

                                                                          ATTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCA                     <  SRR4302534.3650534/94‑1 (MQ=60)

                                                                           TTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCAC                    >  SRR4302534.5571624/1‑94 (MQ=60)

                                                                           TTAACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCAC                    <  SRR4302534.574798/94‑1 (MQ=60)

                                                                             AACCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGG                  >  SRR4302534.231873/1‑94 (MQ=60)

                                                                               CCCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTT                <  SRR4302534.398425/94‑1 (MQ=60)

                                                                                CCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCcggggctttttaaaaaaaatattcgtcctctcccccccccaacaacac               >  SRR4302534.5697048/1‑46 (MQ=60)

                                                                                CCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTA               >  SRR4302534.3000518/1‑94 (MQ=60)

                                                                                CCGACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTA               >  SRR4302534.3513966/1‑94 (MQ=60)

                                                                                  GACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAA             >  SRR4302534.780855/1‑94 (MQ=60)

                                                                                   ACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAAT            <  SRR4302534.6337693/94‑1 (MQ=60)

                                                                                   ACACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAAT            >  SRR4302534.5979770/1‑94 (MQ=60)

                                                                                    CACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATT           <  SRR4302534.1517263/94‑1 (MQ=60)

                                                                                    CACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATT           <  SRR4302534.1268816/94‑1 (MQ=60)

                                                                                    CACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATT           <  SRR4302534.6588504/94‑1 (MQ=60)

                                                                                    CACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATT           <  SRR4302534.2247955/94‑1 (MQ=60)

                                                                                     ACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTG          >  SRR4302534.943108/1‑94 (MQ=60)

                                                                                     ACCCTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTG          >  SRR4302534.3462153/1‑94 (MQ=60)

                                                                                        CTATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCT       >  SRR4302534.183277/1‑94 (MQ=60)

                                                                                        CTATGGCCTGACGGTAACAGAGTTGAATGCCGGAGCACGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCT       <  SRR4302534.3690866/94‑1 (MQ=60)

                                                                                         TATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTT      <  SRR4302534.6018194/94‑1 (MQ=60)

                                                                                         TATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTT      <  SRR4302534.4997294/94‑1 (MQ=60)

                                                                                         TATTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTT      <  SRR4302534.458415/94‑1 (MQ=60)

                                                                                           TTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCT    <  SRR4302534.5453024/94‑1 (MQ=60)

                                                                                           TTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCT    <  SRR4302534.6378848/94‑1 (MQ=60)

                                                                                           TTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCT    <  SRR4302534.5040319/94‑1 (MQ=60)

                                                                                           TTGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCT    >  SRR4302534.4075572/1‑94 (MQ=60)

                                                                                            TGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTG   <  SRR4302534.5306190/94‑1 (MQ=60)

                                                                                            TGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTG   <  SRR4302534.3632395/94‑1 (MQ=60)

                                                                                            TGCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTG   >  SRR4302534.907568/1‑94 (MQ=60)

                                                                                             GCCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGA  >  SRR4302534.3265609/1‑94 (MQ=60)

CTAAATTAGCGTTGCCACCCGATCAGGCCGCAGAAAAATTAAGATTTCGTCGTAGCGCGAATTCTCTGACGCTGATTAACCCGACACCCTATTACCTGACGGTAACAGAGTTGAATGCCGGAACCCGGGTTCTTGAAAATGCATTGGTGCCTCCAATGGGCGAAAGCACGGTTAAATTGCCTTCTGA  >  NC_000913/4542332‑4542518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 7 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`