Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,589,187 A→G intergenic (‑323/‑40) yjiY ← / → tsr putative carbon starvation protein/methyl‑accepting chemotaxis protein I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,589,1870AG100.0% 568.3 / NA 165intergenic (‑323/‑40)yjiY/tsrputative carbon starvation protein/methyl‑accepting chemotaxis protein I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (116/49);  total (116/49)

Only 100 of 168 total aligned reads displayed.

TATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCGT  >  NC_000913/4589098‑4589276
                                                                                         |                                                                                         
  tGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCg                                                                                      >  1:6008996/1‑93 (MQ=255)
   gTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGa                                                                                     <  2:2356541/93‑1 (MQ=255)
    tAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGaa                                                                                    >  1:1359003/1‑93 (MQ=255)
    tAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGaa                                                                                    >  1:5310239/1‑93 (MQ=255)
     aaaTAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGaaa                                                                                   >  2:3729380/1‑93 (MQ=255)
     aaaTAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGaaa                                                                                   <  2:3631387/93‑1 (MQ=255)
      aataatGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGaaaa                                                                                  >  2:1422687/1‑93 (MQ=255)
      aataatGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGaaaa                                                                                  >  1:1651895/1‑93 (MQ=255)
      aataatGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGaaaa                                                                                  >  1:1661693/1‑93 (MQ=255)
        taatGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGCTATTCATAAAGTTTTTCCTTTCCGGGGCGAAAATc                                                                                >  1:3436124/1‑93 (MQ=255)
         aatGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCt                                                                               >  2:5210003/1‑93 (MQ=255)
         aatGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCt                                                                               >  1:1256052/1‑93 (MQ=255)
         aatGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCt                                                                               >  2:2828387/1‑93 (MQ=255)
         aatGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCt                                                                               <  1:804538/93‑1 (MQ=255)
          atGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCtt                                                                              >  2:1783060/1‑93 (MQ=255)
          atGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCtt                                                                              >  1:4985614/1‑93 (MQ=255)
           tGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTg                                                                             <  1:2136359/93‑1 (MQ=255)
             gCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCa                                                                           >  1:6091847/1‑93 (MQ=255)
                ttttAAATTCAGAGTGTGATTAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCg                                                                        >  2:6345722/1‑93 (MQ=255)
                ttttAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCg                                                                        >  2:206673/1‑93 (MQ=255)
                ttttAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCg                                                                        <  1:4112117/93‑1 (MQ=255)
                  ttAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGt                                                                      <  1:1660317/93‑1 (MQ=255)
                   tAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTc                                                                     <  1:5885821/93‑1 (MQ=255)
                   tAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTc                                                                     >  1:5385207/1‑93 (MQ=255)
                   tAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTc                                                                     >  1:1508846/1‑93 (MQ=255)
                     aaTTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCca                                                                   >  1:4856385/1‑93 (MQ=255)
                       ttCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCcaca                                                                 >  1:1543690/1‑93 (MQ=255)
                        tCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAg                                                                <  1:3852682/93‑1 (MQ=255)
                         cAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAgg                                                               >  1:3939091/1‑93 (MQ=255)
                          agagTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGa                                                              >  2:1665331/1‑93 (MQ=255)
                          agagTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGa                                                              >  2:3670570/1‑93 (MQ=255)
                            agTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGaaa                                                            <  2:4393449/93‑1 (MQ=255)
                            agTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGaaa                                                            >  2:3351920/1‑93 (MQ=255)
                             gtgtGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAag                                                           <  2:4569784/93‑1 (MQ=255)
                              tgtgAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAaga                                                          >  2:228720/1‑93 (MQ=255)
                               gtgAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAagag                                                         >  2:3553812/1‑93 (MQ=255)
                               gtgAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAagag                                                         <  2:3351713/93‑1 (MQ=255)
                                tgAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAgaga                                                        >  2:166083/1‑93 (MQ=255)
                                tgAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAgaga                                                        >  1:5866369/1‑93 (MQ=255)
                                  aaTAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGaaa                                                      >  2:6176343/1‑93 (MQ=255)
                                  aaTAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGaaa                                                      <  1:2328117/93‑1 (MQ=255)
                                  aaTAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGaaa                                                      >  1:3600347/1‑93 (MQ=255)
                                  aaTAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGaaa                                                      <  1:3180285/93‑1 (MQ=255)
                                     aaaaTTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCa                                                   <  1:4186000/93‑1 (MQ=255)
                                     aaaaTTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCa                                                   >  1:4833924/1‑93 (MQ=255)
                                         ttCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGtt                                               >  2:158585/1‑93 (MQ=255)
                                           cACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTaa                                             <  1:2203881/93‑1 (MQ=255)
                                           cACTCGGCGTAATCTCCGCAGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTaa                                             >  1:6286921/1‑93 (MQ=255)
                                            aCTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTaaa                                            >  2:3763917/1‑93 (MQ=255)
                                            aCTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTaaa                                            >  2:3174518/1‑93 (MQ=255)
                                              tCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAAc                                          >  1:1047040/1‑93 (MQ=255)
                                                ggCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGt                                        <  2:3990675/93‑1 (MQ=255)
                                                ggCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGt                                        >  2:4255795/1‑93 (MQ=255)
                                                 gCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTa                                       >  2:583135/1‑93 (MQ=255)
                                                    tAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCa                                    >  2:440843/1‑93 (MQ=255)
                                                     aaTCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGATAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCaa                                   >  1:2983292/1‑93 (MQ=255)
                                                     aaTCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCaa                                   >  1:5636060/1‑93 (MQ=255)
                                                      aTCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCaaa                                  >  2:1276361/1‑93 (MQ=255)
                                                      aTCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCaaa                                  >  1:3083078/1‑93 (MQ=255)
                                                         tcCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAAtt                               >  2:1174278/1‑93 (MQ=255)
                                                         tcCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAAtt                               >  1:884525/1‑93 (MQ=255)
                                                         tcCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAAtt                               >  2:3027291/1‑93 (MQ=255)
                                                         tcCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAAtt                               >  1:2036783/1‑93 (MQ=255)
                                                         tcCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAAtt                               >  2:2558207/1‑93 (MQ=255)
                                                         tcCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAAtt                               >  2:2116903/1‑93 (MQ=255)
                                                            gcgGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATtgtg                            >  2:4721890/1‑93 (MQ=255)
                                                              gggATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGAc                          >  1:6427904/1‑93 (MQ=255)
                                                              gggATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGAc                          >  1:6203984/1‑93 (MQ=255)
                                                              gggATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGAc                          <  1:3577536/93‑1 (MQ=255)
                                                               ggATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAGACCATGTTAAAACGTATCAAAATTGTGAcc                         >  2:3835447/1‑93 (MQ=255)
                                                               ggATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGAcc                         >  2:5319950/1‑93 (MQ=255)
                                                               ggATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGAcc                         >  1:701145/1‑93 (MQ=255)
                                                                gATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCa                        >  2:2958587/1‑93 (MQ=255)
                                                                 atatTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAg                       >  1:2508196/1‑93 (MQ=255)
                                                                  tatTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGc                      >  2:2037935/1‑93 (MQ=255)
                                                                   atTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCt                     <  2:3631898/93‑1 (MQ=255)
                                                                    ttCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCtt                    >  2:2483590/1‑93 (MQ=255)
                                                                    ttCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCtt                    >  2:4768214/1‑93 (MQ=255)
                                                                      cATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTAc                  <  1:4463983/93‑1 (MQ=255)
                                                                      cATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTAc                  <  1:4222528/93‑1 (MQ=255)
                                                                          aaGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTActgct              >  1:922885/1‑93 (MQ=255)
                                                                            gTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTgg            >  1:1207610/1‑93 (MQ=255)
                                                                            gTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTgg            >  1:3679342/1‑93 (MQ=255)
                                                                            gTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTgg            <  1:1237606/93‑1 (MQ=255)
                                                                            gTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTgg            >  1:5952710/1‑93 (MQ=255)
                                                                             tttttCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGt           >  2:5725346/1‑93 (MQ=255)
                                                                              ttttCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGtt          >  1:4716567/1‑93 (MQ=255)
                                                                               tttcctttccGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGttt         >  1:3577851/1‑93 (MQ=255)
                                                                               tttcctttccGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGttt         <  2:5913242/93‑1 (MQ=255)
                                                                                 tcctttccGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTg       >  2:5621378/1‑93 (MQ=255)
                                                                                 tcctttccGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTg       <  1:5807728/93‑1 (MQ=255)
                                                                                  cctttccGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTgg      <  1:3423136/93‑1 (MQ=255)
                                                                                  cctttccGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTgg      >  2:4036732/1‑93 (MQ=255)
                                                                                  cctttccGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTgg      >  1:2404494/1‑93 (MQ=255)
                                                                                   ctttccGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGc     >  2:2680806/1‑93 (MQ=255)
                                                                                    tttccGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGcc    >  1:1880961/1‑93 (MQ=255)
                                                                                     ttccGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCg   >  2:2205642/1‑93 (MQ=255)
                                                                                     ttccGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCg   >  1:1047555/1‑93 (MQ=255)
                                                                                      tccGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCGt  >  1:3601021/1‑93 (MQ=255)
                                                                                      tccGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCGt  >  1:3849468/1‑93 (MQ=255)
                                                                                         |                                                                                         
TATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCGT  >  NC_000913/4589098‑4589276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTT  >  NC_000913/4589094‑4589280
                                                                                             |                                                                                             
AATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCG                                                                                               >  SRR4302534.4852081/1‑94 (MQ=60)
AATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCG                                                                                               >  SRR4302534.6574576/1‑94 (MQ=60)
   TTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGC                                                                                            >  SRR4302534.4891218/1‑94 (MQ=60)
     ATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCG                                                                                          >  SRR4302534.6172258/1‑94 (MQ=60)
       GTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAA                                                                                        >  SRR4302534.1394814/1‑94 (MQ=60)
       GTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAA                                                                                        >  SRR4302534.5453517/1‑94 (MQ=60)
         AAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAA                                                                                      >  SRR4302534.1704968/1‑94 (MQ=60)
         AAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAA                                                                                      >  SRR4302534.1694953/1‑94 (MQ=60)
         AAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAA                                                                                      >  SRR4302534.4449181/1‑94 (MQ=60)
           ATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGCTATTCATAAAGTTTTTCCTTTCCGGGGCGAAAATC                                                                                    >  SRR4302534.3528467/1‑94 (MQ=60)
            TAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCT                                                                                   >  SRR4302534.1289149/1‑94 (MQ=60)
            TAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCT                                                                                   >  SRR4302534.1747430/1‑94 (MQ=60)
             AATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTT                                                                                  >  SRR4302534.5120532/1‑94 (MQ=60)
             AATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTT                                                                                  <  SRR4302534.824750/94‑1 (MQ=60)
               TGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGC                                                                                <  SRR4302534.2192685/94‑1 (MQ=60)
               TGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGA                                                                                >  SRR4302534.5344982/1‑94 (MQ=60)
                GGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCA                                                                               >  SRR4302534.6257507/1‑94 (MQ=60)
                GGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCA                                                                               <  SRR4302534.258393/94‑1 (MQ=60)
                    TTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGG                                                                           <  SRR4302534.4222740/94‑1 (MQ=60)
                      TTAAATTCAGAGTGTGAATAAAATTCGCTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTC                                                                         <  SRR4302534.1429360/94‑1 (MQ=60)
                      TTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTC                                                                         <  SRR4302534.1703564/94‑1 (MQ=60)
                      TTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTC                                                                         >  SRR4302534.1548523/1‑94 (MQ=60)
                      TTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTC                                                                         >  SRR4302534.6164148/1‑94 (MQ=60)
                      TTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTC                                                                         >  SRR4302534.3674800/1‑94 (MQ=60)
                      TTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTC                                                                         >  SRR4302534.5530253/1‑94 (MQ=60)
                       TAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCC                                                                        <  SRR4302534.6045635/94‑1 (MQ=60)
                        AAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCA                                                                       >  SRR4302534.4988138/1‑94 (MQ=60)
                          ATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACA                                                                     >  SRR4302534.1584150/1‑94 (MQ=60)
                            TCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGG                                                                   <  SRR4302534.3956119/94‑1 (MQ=60)
                            TCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGG                                                                   >  SRR4302534.4044592/1‑94 (MQ=60)
                              AGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAA                                                                 >  SRR4302534.2194771/1‑94 (MQ=60)
                               GAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAA                                                                <  SRR4302534.1253686/94‑1 (MQ=60)
                               GAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAA                                                                >  SRR4302534.3594897/1‑94 (MQ=60)
                                  TGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAG                                                             >  SRR4302534.856676/1‑94 (MQ=60)
                                   GTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGA                                                            >  SRR4302534.6025655/1‑94 (MQ=60)
                                   GTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGA                                                            <  SRR4302534.6020199/94‑1 (MQ=60)
                                     GAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAA                                                          >  SRR4302534.3697175/1‑94 (MQ=60)
                                     GAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAA                                                          >  SRR4302534.4136554/1‑94 (MQ=60)
                                      AATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAAC                                                         <  SRR4302534.3264293/94‑1 (MQ=60)
                                      AATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAAC                                                         <  SRR4302534.2389890/94‑1 (MQ=60)
                                        TAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCA                                                       >  SRR4302534.4965077/1‑94 (MQ=60)
                                        TAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGGTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCA                                                       >  SRR4302534.1621439/1‑94 (MQ=60)
                                         AAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCAT                                                      <  SRR4302534.4299132/94‑1 (MQ=60)
                                         AAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCAT                                                      <  SRR4302534.6527539/94‑1 (MQ=60)
                                            ATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTT                                                   >  SRR4302534.6401253/1‑94 (MQ=60)
                                              TCACTCGGCGTAATCTCCGCAGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAA                                                 >  SRR4302534.6457020/1‑94 (MQ=60)
                                               CACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAA                                                <  SRR4302534.2262199/94‑1 (MQ=60)
                                                 CTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAAC                                              >  SRR4302534.1074118/1‑94 (MQ=60)
                                                  TCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACG                                             <  SRR4302534.3820669/94‑1 (MQ=60)
                                                       GTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCA                                        >  SRR4302534.116598/1‑94 (MQ=60)
                                                        TAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGATAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAA                                       >  SRR4302534.3061342/1‑94 (MQ=60)
                                                        TAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAA                                       >  SRR4302534.5788607/1‑94 (MQ=60)
                                                         AATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAA                                      >  SRR4302534.4984326/1‑94 (MQ=60)
                                                         AATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAA                                      >  SRR4302534.3164085/1‑94 (MQ=60)
                                                          ATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAA                                     >  SRR4302534.5491106/1‑94 (MQ=60)
                                                            CTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATT                                   >  SRR4302534.906907/1‑94 (MQ=60)
                                                            CTCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATT                                   >  SRR4302534.2090042/1‑94 (MQ=60)
                                                             TCCGCGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTG                                  >  SRR4302534.3985697/1‑94 (MQ=60)
                                                                 CGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGAC                              >  SRR4302534.6601236/1‑94 (MQ=60)
                                                                 CGGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGAC                              >  SRR4302534.6372686/1‑94 (MQ=60)
                                                                  GGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACC                             >  SRR4302534.5937176/1‑94 (MQ=60)
                                                                  GGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACC                             <  SRR4302534.3673761/94‑1 (MQ=60)
                                                                  GGGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACC                             >  SRR4302534.718329/1‑94 (MQ=60)
                                                                   GGATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCA                            <  SRR4302534.6112824/94‑1 (MQ=60)
                                                                    GATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAG                           <  SRR4302534.3322582/94‑1 (MQ=60)
                                                                    GATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAG                           >  SRR4302534.2574795/1‑94 (MQ=60)
                                                                    GATATTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAG                           <  SRR4302534.1702429/94‑1 (MQ=60)
                                                                        TTCATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACAAGCTTA                       >  SRR4302534.1387591/1‑94 (MQ=60)
                                                                          CATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACT                     <  SRR4302534.4336351/94‑1 (MQ=60)
                                                                          CATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACT                     <  SRR4302534.4584685/94‑1 (MQ=60)
                                                                          CATAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACT                     >  SRR4302534.4306154/1‑94 (MQ=60)
                                                                            TAAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGC                   <  SRR4302534.235015/94‑1 (MQ=60)
                                                                             AAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCGGCTTACTGCT                  >  SRR4302534.3762512/1‑94 (MQ=60)
                                                                             AAAGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCT                  >  SRR4302534.946390/1‑94 (MQ=60)
                                                                               AGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGG                >  SRR4302534.3778260/1‑94 (MQ=60)
                                                                               AGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGG                >  SRR4302534.6114343/1‑94 (MQ=60)
                                                                               AGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGG                >  SRR4302534.1239386/1‑94 (MQ=60)
                                                                               AGTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGCTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGG                >  SRR4302534.5905213/1‑94 (MQ=60)
                                                                                GTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGT               <  SRR4302534.6351177/94‑1 (MQ=60)
                                                                                GTTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGT               <  SRR4302534.1270231/94‑1 (MQ=60)
                                                                                 TTTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTT              >  SRR4302534.4844644/1‑94 (MQ=60)
                                                                                  TTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTT             >  SRR4302534.3674087/1‑94 (MQ=60)
                                                                                  TTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTT             >  SRR4302534.3674086/1‑94 (MQ=60)
                                                                                  TTTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTT             >  SRR4302534.3197540/1‑94 (MQ=60)
                                                                                   TTTCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTT            <  SRR4302534.2064981/94‑1 (MQ=60)
                                                                                     TCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGG          <  SRR4302534.5965359/94‑1 (MQ=60)
                                                                                     TCCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGG          >  SRR4302534.2468396/1‑94 (MQ=60)
                                                                                      CCTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGC         <  SRR4302534.3515111/94‑1 (MQ=60)
                                                                                       CTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCC        >  SRR4302534.1929958/1‑94 (MQ=60)
                                                                                       CTTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCC        <  SRR4302534.773547/94‑1 (MQ=60)
                                                                                        TTTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCG       >  SRR4302534.1074647/1‑94 (MQ=60)
                                                                                         TTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCGT      <  SRR4302534.2516924/94‑1 (MQ=60)
                                                                                         TTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCGT      >  SRR4302534.3697861/1‑94 (MQ=60)
                                                                                         TTCCGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCGT      >  SRR4302534.3952839/1‑94 (MQ=60)
                                                                                            CGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCGTTTT   >  SRR4302534.3465266/1‑94 (MQ=60)
                                                                                            CGGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCGTTTT   <  SRR4302534.3218987/94‑1 (MQ=60)
                                                                                             GGGCCGAAAATCTTGCATCGGTCCACAGGGAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCGgtgtt  >  SRR4302534.485316/1‑89 (MQ=60)
                                                                                             GGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTT  >  SRR4302534.2658484/1‑94 (MQ=60)
                                                                                             |                                                                                             
AATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACAGGAAAGAGAAACCATGTTAAAACGTATCAAAATTGTGACCAGCTTACTGCTGGTTTTGGCCGTTTTT  >  NC_000913/4589094‑4589280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑