Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,593,254 2 bp→GC coding (59‑60/807 nt) yjjM ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,593,2540CG100.0% 733.3 / NA 211A20G (GCG→GGC) yjjMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (106/105);  total (106/105)
*NC_0009134,593,2550GC100.0% 725.6 / NA 211A20G (GCG→GGC) yjjMhypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (106/105);  total (106/105)

Only 100 of 213 total aligned reads displayed.

ACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGAAGT  >  NC_000913/4593167‑4593340
                                                                                       ||                                                                                     
aCTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCa                                                                                   <  1:1020046/93‑1 (MQ=255)
aCTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCa                                                                                   <  2:1779279/93‑1 (MQ=255)
 cTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAt                                                                                  >  2:2903474/1‑93 (MQ=255)
 cTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAt                                                                                  >  2:467334/1‑93 (MQ=255)
  tCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAtt                                                                                 >  2:789571/1‑93 (MQ=255)
  tCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAtt                                                                                 <  1:524790/93‑1 (MQ=255)
  tCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAtt                                                                                 >  1:6361161/1‑93 (MQ=255)
   cATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTc                                                                                <  2:4647093/93‑1 (MQ=255)
    aTTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTcg                                                                               <  2:2517969/93‑1 (MQ=255)
       gAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTTCGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGt                                                                            >  1:5436071/1‑93 (MQ=255)
       gAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGt                                                                            <  1:4640896/93‑1 (MQ=255)
         aGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTaa                                                                          >  2:5190232/1‑93 (MQ=255)
           cGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGt                                                                        <  1:482596/93‑1 (MQ=255)
            ggTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTg                                                                       >  1:6431532/1‑93 (MQ=255)
              tagtaCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGc                                                                     <  2:2397698/93‑1 (MQ=255)
              tagtaCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGc                                                                     >  1:2495200/1‑93 (MQ=255)
               agtaCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCt                                                                    >  2:5395521/1‑93 (MQ=255)
                 taCAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTga                                                                  >  1:1804111/1‑93 (MQ=255)
                    aGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGaa                                                               <  1:2925731/93‑1 (MQ=255)
                    aGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGaa                                                               <  1:4135932/93‑1 (MQ=255)
                     ggCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAat                                                              >  1:2020078/1‑93 (MQ=255)
                     ggCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAat                                                              <  2:456637/93‑1 (MQ=255)
                      gCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAata                                                             <  2:3696278/93‑1 (MQ=255)
                        gAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATg                                                           >  2:3405751/1‑93 (MQ=255)
                         aaaCCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGa                                                          <  1:768706/93‑1 (MQ=255)
                          aaCCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAc                                                         >  1:4803384/1‑93 (MQ=255)
                           aCCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACg                                                        >  1:2662179/1‑93 (MQ=255)
                             ggTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCa                                                      >  1:6200982/2‑93 (MQ=255)
                              gTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCAc                                                     >  2:2157555/1‑93 (MQ=255)
                                catcatGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtg                                                   >  1:4591748/1‑93 (MQ=255)
                                catcatGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtg                                                   <  2:6098116/93‑1 (MQ=255)
                                 atcatGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtgt                                                  <  2:3261378/93‑1 (MQ=255)
                                 atcatGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtgt                                                  <  1:5064767/93‑1 (MQ=255)
                                  tcatGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACtgtg                                                 <  2:2823785/93‑1 (MQ=255)
                                   catGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTgtgg                                                <  2:4253242/93‑1 (MQ=255)
                                   catGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTgtgg                                                >  1:4784697/1‑93 (MQ=255)
                                    atGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTgtggt                                               >  2:5608034/1‑93 (MQ=255)
                                    atGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTgtggt                                               <  2:4874684/93‑1 (MQ=255)
                                     tGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTgtggtg                                              <  2:158958/93‑1 (MQ=255)
                                        tCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCgg                                           <  2:3980321/93‑1 (MQ=255)
                                          aGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTTGTGCGGCt                                         >  1:316573/1‑93 (MQ=255)
                                          aGGTTTACGCGCAATAACATAGTCGCTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCt                                         >  1:1499554/1‑93 (MQ=255)
                                             tttACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAt                                      <  2:1153858/93‑1 (MQ=255)
                                               tACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtgt                                    <  1:5725109/93‑1 (MQ=255)
                                                 cgcgCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtgttg                                  >  1:5256640/1‑93 (MQ=255)
                                                 cgcgCAATAACATAGTCGTTGCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGCCGCACTGTGGTGCGGCTGAtgttg                                  <  2:6451237/93‑1 (MQ=255)
                                                  gcgcAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtgttgt                                 >  2:619653/1‑93 (MQ=255)
                                                  gcgcAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAtgttgt                                 <  1:3972069/93‑1 (MQ=255)
                                                   cgcAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTa                                <  2:5048890/93‑1 (MQ=255)
                                                     cAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACa                              <  1:5662679/93‑1 (MQ=255)
                                                     cAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACa                              <  2:5824718/93‑1 (MQ=255)
                                                     cAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACa                              <  1:3829297/93‑1 (MQ=255)
                                                      aaTAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAt                             <  2:5770499/93‑1 (MQ=255)
                                                      aaTAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAt                             >  1:577588/1‑93 (MQ=255)
                                                      aaTAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAGTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAt                             <  2:3121565/93‑1 (MQ=255)
                                                       aTAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAtt                            >  1:1551992/1‑93 (MQ=255)
                                                        tAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAttt                           >  1:2714092/1‑93 (MQ=255)
                                                          aCATAGTCGTTTGCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTAGATTTcc                         >  1:1406493/1‑93 (MQ=255)
                                                          aCATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTcc                         <  2:1160133/93‑1 (MQ=255)
                                                          aCATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTcc                         >  2:4390769/1‑93 (MQ=255)
                                                          aCATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTcc                         <  1:3085667/93‑1 (MQ=255)
                                                           cATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCg                        >  1:1315112/1‑93 (MQ=255)
                                                           cATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCg                        >  1:186480/1‑93 (MQ=255)
                                                           cATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCg                        >  2:4269865/1‑93 (MQ=255)
                                                           cATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCg                        >  1:5532674/1‑93 (MQ=255)
                                                            aTAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGc                       <  2:5436375/93‑1 (MQ=255)
                                                             tAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGcc                      <  1:2686114/93‑1 (MQ=255)
                                                               gTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAg                    >  2:3013584/1‑93 (MQ=255)
                                                               gTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAg                    >  2:2582995/1‑93 (MQ=255)
                                                                gcGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAgc                   <  1:4056787/92‑1 (MQ=255)
                                                                  gTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAgcgc                 >  1:4706671/1‑93 (MQ=255)
                                                                   tttCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCa                >  1:3991142/1‑93 (MQ=255)
                                                                   tttCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCa                <  1:5291113/93‑1 (MQ=255)
                                                                    ttCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCat               >  2:2024835/1‑92 (MQ=255)
                                                                    ttCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCAc               >  2:459422/1‑93 (MQ=255)
                                                                     tCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACt              <  2:5120575/93‑1 (MQ=255)
                                                                     tCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACt              >  2:1220791/1‑93 (MQ=255)
                                                                     tCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACt              <  2:3536454/93‑1 (MQ=255)
                                                                      ccAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACtt             <  1:2415628/93‑1 (MQ=255)
                                                                      ccAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACtt             >  2:5348369/1‑93 (MQ=255)
                                                                      ccAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACtt             >  1:3568295/1‑93 (MQ=255)
                                                                      ccAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACtt             <  2:5073632/93‑1 (MQ=255)
                                                                       cAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACttt            >  1:159584/1‑93 (MQ=255)
                                                                       cAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACttt            <  1:4310343/93‑1 (MQ=255)
                                                                        aaCCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTg           >  2:906429/1‑93 (MQ=255)
                                                                         aCCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGc          <  2:2646944/93‑1 (MQ=255)
                                                                          ccTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCg         >  2:5971861/1‑93 (MQ=255)
                                                                            tGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCTCACTTTGCGAt       >  2:1631892/1‑93 (MQ=255)
                                                                            tGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAt       >  2:2895056/1‑93 (MQ=255)
                                                                             gCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATg      <  1:145753/93‑1 (MQ=255)
                                                                             gCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATg      <  2:882695/93‑1 (MQ=255)
                                                                              cGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATgg     >  1:4867079/1‑92 (MQ=255)
                                                                              cGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATgg     <  1:2866268/93‑2 (MQ=255)
                                                                               gTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATgga    >  2:5734634/1‑91 (MQ=255)
                                                                               gTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATgga    >  1:3242134/1‑91 (MQ=255)
                                                                                tCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATggaa   >  2:3772583/1‑90 (MQ=255)
                                                                                tCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATggaa   >  1:5792154/1‑90 (MQ=255)
                                                                                tCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATggaa   >  1:1790379/1‑90 (MQ=255)
                                                                                 cAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATggaag  <  2:3846396/93‑5 (MQ=255)
                                                                                 cAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATggaag  <  1:112006/93‑5 (MQ=255)
                                                                                       ||                                                                                     
ACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGAAGT  >  NC_000913/4593167‑4593340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GAAGTGG  >  NC_000913/4593161‑4593342
                                                                                             |                                                                                         
TTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACG                                                                                           >  SRR4302534.4544640/1‑94 (MQ=60)
TTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACG                                                                                           >  SRR4302534.4883631/1‑94 (MQ=60)
TTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACG                                                                                           <  SRR4302534.3905016/94‑1 (MQ=60)
TTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACG                                                                                           <  SRR4302534.381938/94‑1 (MQ=60)
     CACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCA                                                                                      <  SRR4302534.3494836/94‑1 (MQ=60)
     CACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCA                                                                                      <  SRR4302534.329648/94‑1 (MQ=60)
     CACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCA                                                                                      <  SRR4302534.3174333/94‑1 (MQ=60)
      ACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAT                                                                                     <  SRR4302534.1046305/94‑1 (MQ=60)
      ACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCAT                                                                                     <  SRR4302534.2509655/94‑1 (MQ=60)
       CTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATT                                                                                    >  SRR4302534.6532949/1‑94 (MQ=60)
       CTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATT                                                                                    <  SRR4302534.3078485/94‑1 (MQ=60)
        TCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTC                                                                                   <  SRR4302534.537834/94‑1 (MQ=60)
            TGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTTCGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGT                                                                               >  SRR4302534.5582455/1‑94 (MQ=60)
            TGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGT                                                                               >  SRR4302534.2781165/1‑94 (MQ=60)
             GAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTA                                                                              <  SRR4302534.5359045/94‑1 (MQ=60)
             GAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTA                                                                              <  SRR4302534.1290266/94‑1 (MQ=60)
             GAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTA                                                                              <  SRR4302534.4766828/94‑1 (MQ=60)
              AAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAA                                                                             <  SRR4302534.5948403/94‑1 (MQ=60)
              AAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAA                                                                             >  SRR4302534.668402/1‑94 (MQ=60)
               AGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAG                                                                            >  SRR4302534.5033066/1‑94 (MQ=60)
               AGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAG                                                                            >  SRR4302534.1509436/1‑94 (MQ=60)
                 CGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTG                                                                          <  SRR4302534.494694/94‑1 (MQ=60)
                 CGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTG                                                                          >  SRR4302534.6604921/1‑94 (MQ=60)
                   GTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGC                                                                        >  SRR4302534.2561473/1‑94 (MQ=60)
                      GTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGA                                                                     >  SRR4302534.1850960/1‑94 (MQ=60)
                      GTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGA                                                                     >  SRR4302534.4063354/1‑94 (MQ=60)
                       TACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAG                                                                    <  SRR4302534.3225974/94‑1 (MQ=60)
                       TACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAG                                                                    <  SRR4302534.396038/94‑1 (MQ=60)
                          AGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAAT                                                                 >  SRR4302534.1349929/1‑94 (MQ=60)
                          AGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAAT                                                                 <  SRR4302534.4341877/94‑1 (MQ=60)
                          AGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAAT                                                                 >  SRR4302534.2072934/1‑94 (MQ=60)
                          AGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAAT                                                                 <  SRR4302534.4247522/94‑1 (MQ=60)
                          AGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAAT                                                                 <  SRR4302534.3002535/94‑1 (MQ=60)
                          AGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAAT                                                                 >  SRR4302534.1839683/1‑94 (MQ=60)
                          AGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAAT                                                                 >  SRR4302534.1748185/1‑94 (MQ=60)
                            GCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATAT                                                               >  SRR4302534.6397164/1‑94 (MQ=60)
                               AAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAC                                                            >  SRR4302534.4933810/1‑94 (MQ=60)
                               AAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAC                                                            >  SRR4302534.484958/1‑94 (MQ=60)
                               AAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAC                                                            <  SRR4302534.787816/94‑1 (MQ=60)
                               AAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGAC                                                            >  SRR4302534.1899871/1‑94 (MQ=60)
                                AACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACG                                                           >  SRR4302534.2732558/1‑94 (MQ=60)
                                AACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACG                                                           >  SRR4302534.1458846/1‑94 (MQ=60)
                                 ACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGC                                                          >  SRR4302534.3273088/1‑94 (MQ=60)
                                  CGGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCA                                                         >  SRR4302534.6369641/1‑94 (MQ=60)
                                   CGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCAC                                                        <  SRR4302534.41540/94‑1 (MQ=60)
                                   CGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCAC                                                        <  SRR4302534.1790893/94‑1 (MQ=60)
                                   CGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCAC                                                        >  SRR4302534.4592929/1‑94 (MQ=60)
                                    GTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACT                                                       <  SRR4302534.3851287/94‑1 (MQ=60)
                                     TCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTG                                                      >  SRR4302534.4716362/1‑94 (MQ=60)
                                       ATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTG                                                    <  SRR4302534.5201514/94‑1 (MQ=60)
                                       ATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTG                                                    <  SRR4302534.2750107/94‑1 (MQ=60)
                                        TCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGG                                                   >  SRR4302534.4914631/1‑94 (MQ=60)
                                          ATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTG                                                 <  SRR4302534.1141446/94‑1 (MQ=60)
                                           TGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGC                                                <  SRR4302534.2989826/94‑1 (MQ=60)
                                            GGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCG                                               <  SRR4302534.5422490/94‑1 (MQ=60)
                                            GGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCG                                               <  SRR4302534.4809884/94‑1 (MQ=60)
                                             GTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGCGAATATGACGCACTGTGGCGCGG                                              >  SRR4302534.4928540/1‑94 (MQ=60)
                                             GTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGG                                              <  SRR4302534.2599076/94‑1 (MQ=60)
                                               CAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTTGTGCGGCT                                            >  SRR4302534.324727/1‑94 (MQ=60)
                                               CAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCT                                            <  SRR4302534.2775694/94‑1 (MQ=60)
                                               CAGGTTTACGCGCAATAACATAGTCGCTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCT                                            >  SRR4302534.1539018/1‑94 (MQ=60)
                                                AGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTG                                           <  SRR4302534.59698/94‑1 (MQ=60)
                                                AGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTG                                           >  SRR4302534.586595/1‑94 (MQ=60)
                                                  GTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGAT                                         <  SRR4302534.1597916/94‑1 (MQ=60)
                                                   TTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATG                                        <  SRR4302534.1013015/94‑1 (MQ=60)
                                                   TTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATG                                        <  SRR4302534.493648/94‑1 (MQ=60)
                                                     TACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTT                                      <  SRR4302534.5880216/94‑1 (MQ=60)
                                                      ACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTG                                     >  SRR4302534.5398688/1‑94 (MQ=60)
                                                      ACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTG                                     >  SRR4302534.1983874/1‑94 (MQ=60)
                                                        GCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAATTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTA                                   <  SRR4302534.5391695/94‑1 (MQ=60)
                                                        GCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTA                                   <  SRR4302534.4078388/94‑1 (MQ=60)
                                                          GCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACC                                 <  SRR4302534.4177884/94‑1 (MQ=60)
                                                          GCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACA                                 >  SRR4302534.577170/1‑94 (MQ=60)
                                                          GCAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACA                                 <  SRR4302534.6540910/94‑1 (MQ=60)
                                                           CAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAT                                <  SRR4302534.5815983/94‑1 (MQ=60)
                                                           CAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAT                                <  SRR4302534.3932145/94‑1 (MQ=60)
                                                           CAATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACAT                                >  SRR4302534.591749/1‑94 (MQ=60)
                                                            AATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATT                               <  SRR4302534.5876439/94‑1 (MQ=60)
                                                            AATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATT                               >  SRR4302534.4808848/1‑94 (MQ=60)
                                                            AATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATT                               >  SRR4302534.1592646/1‑94 (MQ=60)
                                                             ATAACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTT                              >  SRR4302534.2785742/1‑94 (MQ=60)
                                                               AACATAGTCGTTTGCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTAGATTTCC                            >  SRR4302534.1443487/1‑94 (MQ=60)
                                                               AACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCC                            <  SRR4302534.4093250/94‑1 (MQ=60)
                                                                ACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCG                           <  SRR4302534.3166752/94‑1 (MQ=60)
                                                                ACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCG                           >  SRR4302534.5681999/1‑94 (MQ=60)
                                                                ACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCG                           >  SRR4302534.191374/1‑94 (MQ=60)
                                                                ACATAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCG                           >  SRR4302534.1349785/1‑94 (MQ=60)
                                                                   TAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCA                        <  SRR4302534.2757107/94‑1 (MQ=60)
                                                                   TAGTCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCA                        <  SRR4302534.5399581/94‑1 (MQ=60)
                                                                      GCGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCG                     <  SRR4302534.4165600/94‑1 (MQ=60)
                                                                       CGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGATAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGC                    >  SRR4302534.154290/1‑94 (MQ=60)
                                                                       CGTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGC                    >  SRR4302534.4834448/1‑94 (MQ=60)
                                                                        GTTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCA                   >  SRR4302534.4097966/1‑94 (MQ=60)
                                                                         TTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCAC                  >  SRR4302534.6427964/1‑94 (MQ=60)
                                                                         TTTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCAC                  <  SRR4302534.5433915/94‑1 (MQ=60)
                                                                          TTCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACT                 >  SRR4302534.3651699/1‑94 (MQ=60)
                                                                           TCCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTT                >  SRR4302534.3664274/1‑94 (MQ=60)
                                                                            CCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTT               >  SRR4302534.4759728/1‑94 (MQ=60)
                                                                            CCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTT               <  SRR4302534.2479871/94‑1 (MQ=60)
                                                                            CCAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTT               >  SRR4302534.163758/1‑94 (MQ=60)
                                                                             CAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTG              <  SRR4302534.4427121/94‑1 (MQ=60)
                                                                             CAACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTG              >  SRR4302534.2479967/1‑94 (MQ=60)
                                                                              AACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGC             >  SRR4302534.5489407/1‑94 (MQ=60)
                                                                              AACCTGCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGC             <  SRR4302534.2870406/94‑1 (MQ=60)
                                                                                   GCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GG       <  SRR4302534.5605161/94‑1 (MQ=60)
                                                                                   GCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GG       <  SRR4302534.4986221/94‑1 (MQ=60)
                                                                                   GCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GG       <  SRR4302534.149549/94‑1 (MQ=60)
                                                                                   GCGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GG       >  SRR4302534.4999103/1‑94 (MQ=60)
                                                                                    CGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GGA      <  SRR4302534.2941547/94‑1 (MQ=60)
                                                                                    CGTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GGA      >  SRR4302534.3328408/1‑94 (MQ=60)
                                                                                     GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaa     >  SRR4302534.5949303/1‑91 (MQ=60)
                                                                                     GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaa     >  SRR4302534.1836877/1‑91 (MQ=60)
                                                                                     GTCAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaa     <  SRR4302534.2272812/94‑4 (MQ=60)
                                                                                       CAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGT    <  SRR4302534.114998/94‑1 (MQ=60)
                                                                                       CAAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑Ggaagg   >  SRR4302534.6496220/1‑89 (MQ=60)
                                                                                        AAGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTG   >  SRR4302534.5564132/1‑94 (MQ=60)
                                                                                         AGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGG  <  SRR4302534.3395517/94‑1 (MQ=60)
                                                                                         AGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGG  <  SRR4302534.3798797/94‑1 (MQ=60)
                                                                                         AGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGG  >  SRR4302534.697237/1‑94 (MQ=60)
                                                                                         AGACGCCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGATGGAAGTGG  >  SRR4302534.979552/1‑94 (MQ=60)
                                                                                             |                                                                                         
TTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCGTCATCATGGTCAGGTTTACGCGCAATAACATAGTCGTTTCCAACCTGCGTCAAGACCGCGCATTCGCGTAAGTGGCTGAGAATATGACGCACTGTGGTGCGGCTGATGTTGTACATTTCCGCCAGCGCACTTTGCGAT‑GAAGTGG  >  NC_000913/4593161‑4593342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑