Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I0 R3	223	74.2	2572381	97.5%	2508071	179.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	AE015451	4,741,278	C→T	100%	intergenic (+515/+238)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451	4,741,278	0	C	T	100.0%	285.0 / ‑6.8	74	intergenic (+515/+238)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (36/37); minor base G (0/1); total (36/38)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTTCCGGCAGGTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACCTCC‑GCCAAC‑TGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTC‑C‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATACGCTTGAACGATCTCATCATGTTTATCCTTGTTGT  >  AE015451/4741072‑4741500

ctTCCGGCAGGTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAg                                                                                                                                                                                                     >  2:1011584/1‑235 (MQ=255)

 ttCCGGCAGCTCAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCa                                                                                                                                                                                                      <  1:834158/233‑1 (MQ=255)

         ggTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGgcgc                                                                                                                                                                                               >  2:673713/1‑232 (MQ=255)

             attCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATc                                                                                                                                                                                        <  1:491475/233‑1 (MQ=255)

               tCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCAc                                                                                                                                                                                      <  1:182985/235‑1 (MQ=255)

                 ggCTCGTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGtcctc‑ct                                                                                                                                                                                                                           <  1:65049/196‑2 (MQ=255)

                  gCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTc                                                                                                                                                                                  <  1:673716/236‑1 (MQ=255)

                    tcttctTCCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                                                                                                                                 <  1:254810/235‑1 (MQ=255)

                            cAGTTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                                                                                                                                         >  2:633773/1‑235 (MQ=255)

                            cAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATCGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                                                                                                                                 <  2:536016/227‑1 (MQ=255)

                                ttACGGGTCAGGCTGCCATCCGCCTCCTTGATTGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                                                                                                                                                     >  1:420541/1‑236 (MQ=255)

                                            cTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                                                                                                                                                          >  2:991576/1‑234 (MQ=255)

                                                cctCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                                                                                                                                    <  2:941281/218‑1 (MQ=255)

                                                         cctTGA‑TGGTCGTAATGTGCATCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTTGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGgcgc                                                                                                                                                                                               <  1:457545/184‑1 (MQ=255)

                                                             gA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGa                                                                                                                                       >  1:895503/1‑236 (MQ=255)

                                                              a‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAg                                                                                                                                      >  1:65812/1‑236 (MQ=255)

                                                              a‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCATGCTCGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAg                                                                                                                                      <  1:652571/236‑1 (MQ=255)

                                                                   tCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGATCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTCAT‑GCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTg                                                                                                                                        <  2:650861/230‑1 (MQ=255)

                                                                        aTGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAccc                                                                                                                                                     >  1:992474/1‑212 (MQ=255)

                                                                        aTGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAccc                                                                                                                                                     <  2:992471/212‑1 (MQ=255)

                                                                               gcaCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTg                                                                                                                                                          <  2:929013/200‑1 (MQ=255)

                                                                                caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCa                                                                                                                                                                                       >  2:69224/1‑170 (MQ=255)

                                                                                 aCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCTGCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgt                                                                                                                     <  1:1011587/235‑1 (MQ=255)

                                                                                   ggAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtc                                                                                                                    >  2:1106805/1‑234 (MQ=255)

                                                                                   ggAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCTAGCGGCGCCTTGATCACGGTCACACcgc                                                                                                                                                                           >  1:356505/1‑179 (MQ=255)

                                                                                    gAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcct                                                                                                                  >  1:712245/1‑235 (MQ=255)

                                                                                        cACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcgtcc                                                                                                             >  1:425249/1‑236 (MQ=255)

                                                                                          cgcgGCGTAACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc                                                                                                                                                                           <  2:594219/172‑1 (MQ=255)

                                                                                            cggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCgg                                                                                                                                                   <  2:1205137/194‑1 (MQ=255)

                                                                                             ggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCCCCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCg                                                                                                                                                    <  2:186512/192‑1 (MQ=255)

                                                                                             ggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGc                                                                                                        >  1:614084/1‑236 (MQ=255)

                                                                                             ggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGc                                                                                                        >  1:473379/1‑236 (MQ=255)

                                                                                             ggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAc                                                                                                                                                       >  1:6783/1‑189 (MQ=255)

                                                                                             ggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGa                                                                                                                                   >  1:483893/1‑209 (MQ=255)

                                                                                              gcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCa                                                                                                                                                <  2:483890/195‑1 (MQ=255)

                                                                                                  cACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACt                                                                                                    >  1:484650/1‑235 (MQ=255)

                                                                                                  cACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTg                                                                                                                                        <  1:634214/199‑1 (MQ=255)

                                                                                                   aCCGGACATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAc                                                                                                                                          <  2:1073924/196‑1 (MQ=255)

                                                                                                    ccGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtt                                                                                                                                                                                            <  2:141701/145‑1 (MQ=255)

                                                                                                      ggCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCt                                                                                                                                             <  1:25080/190‑1 (MQ=255)

                                                                                                           tGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTTCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCa                                                                                                                                                <  2:519745/182‑1 (MQ=255)

                                                                                                            ggCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGttt                                                                                           >  2:548128/1‑234 (MQ=255)

                                                                                                               gTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCCCTTGCCAGt                                                                                             >  2:936479/1‑229 (MQ=255)

                                                                                                               gTATTGAACT‑GGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGcc                                                                                     >  1:823086/1‑236 (MQ=255)

                                                                                                                  ttGACCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGCCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAgcggcg                                                                                                                                                                                                <  2:81494/127‑1 (MQ=255)

                                                                                                                     aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACCGTCACACCGCGAt                                                                                                                                                                        >  2:108258/1‑148 (MQ=255)

                                                                                                                            tataGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCcaca                                                                            <  2:425246/233‑1 (MQ=255)

                                                                                                                             ataGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCT‑GCGACT‑TGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAg                                                                           >  2:930868/1‑233 (MQ=255)

                                                                                                                                    cAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGACTTGC‑ATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAc                                                                      <  1:1220645/231‑1 (MQ=255)

                                                                                                                                    cAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAc                                                                      >  2:1220642/1‑231 (MQ=255)

                                                                                                                                     aGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgt                                                                                                                     <  1:1136058/183‑1 (MQ=255)

                                                                                                                                       ctctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGACTTGC‑ATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAcag                                                                    >  2:451747/1‑230 (MQ=255)

                                                                                                                                       ctctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGcc                                                                                                       >  2:764612/1‑195 (MQ=255)

                                                                                                                                       ctctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCa                                                                                   >  1:16568/1‑215 (MQ=255)

                                                                                                                                       ctctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGaa                                                               >  1:70371/1‑235 (MQ=255)

                                                                                                                                       ctctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTAGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAc                                                                      >  1:978627/1‑228 (MQ=255)

                                                                                                                                       ctctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATCCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCtag                                                                                                                                                                                           >  2:357670/1‑109 (MQ=255)

                                                                                                                                        tctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACCCCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAgg                                                                          >  2:777113/1‑223 (MQ=255)

                                                                                                                                        tctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TGTCACTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAcag                                                                    <  1:369432/229‑1 (MQ=255)

                                                                                                                                         ctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAcagcag                                                                 >  1:1147505/1‑231 (MQ=255)

                                                                                                                                         ctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAcagc                                                                   >  1:284103/1‑229 (MQ=255)

                                                                                                                                         ctGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAacac                                                            >  1:1190014/1‑236 (MQ=255)

                                                                                                                                          tGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGCA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAacac                                                            <  1:137528/235‑1 (MQ=255)

                                                                                                                                          tGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAacac                                                            <  1:737887/235‑1 (MQ=255)

                                                                                                                                          tGGTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAacac                                                            <  1:474295/235‑1 (MQ=255)

                                                                                                                                           ggTCACCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAcag                                                                    <  2:887522/226‑1 (MQ=255)

                                                                                                                                               aCCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGGCACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGAcagc                                                                   >  1:332747/1‑223 (MQ=255)

                                                                                                                                               aCCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAgg                                                                          <  2:528270/216‑1 (MQ=255)

                                                                                                                                               aCCTTCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGAGATAGGCCTTGTACTTGGAc                                                                                                                                                       >  2:1270573/1‑139 (MQ=255)

                                                                                                                                                   tCGTGCTTGAG‑CAGCTTC‑TGCGAC‑TTGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACccacacc                                                     <  2:823083/233‑1 (MQ=255)

                                                                                                                                                                        tgcgacttgcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATACGCtt                                <  1:996637/226‑1 (MQ=255)

                                                                                                                                                                                gcaTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGt                                                                                             <  2:468868/165‑1 (MQ=255)

                                                                                                                                                                                  aTTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGt                                           <  1:669276/215‑1 (MQ=255)

                                                                                                                                                                                             ccaggcacaGGCCCCGGACACGTCCTC‑CTGCAGCGCCTCGACCTTAGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACccacaccaca                                                  <  1:983626/190‑1 (MQ=255)

                                                                                                                                                                                                       gCCCCGGACACGGCCTC‑CTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATACGCTTGAACGATCTCATCATGTTTATCCTtgttgt  <  1:1052776/236‑1 (MQ=255)

CTTCCGGCAGGTAAGTCGGCTCTTCTTTCAGGTTACGGGTCAGGCTGCCATCCGCCTCCTTGA‑TGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AACCTCC‑GCCAAC‑TGCAATTCGTGAA‑CCAAGCAAAAGGCCCCGAAAAGGCCCTC‑C‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCCAGGCCACAGGCGACAGCAGAACACCCACACCACACAGCAGTGCAATACGCTTGAACGATCTCATCATGTTTATCCTTGTTGT  >  AE015451/4741072‑4741500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACCTCCGCCAACTGCAATTCGTGAACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCC  >  AE015451/4741119‑4741418

CCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAG                                                    >  ERR1711319.907403/1‑251 (MQ=60)

 CATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGG                                                   >  ERR1711319.66444/1‑251 (MQ=60)

          CCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGActgtctcttat                                          >  ERR1711319.1005356/1‑240 (MQ=60)

              GATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCATGCTCGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCC                                      <  ERR1711319.660893/251‑1 (MQ=60)

                      TAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTC                               >  ERR1711319.721862/1‑250 (MQ=60)

                          GTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCT                          >  ERR1711319.430711/1‑251 (MQ=60)

                               GCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCC                     >  ERR1711319.479372/1‑251 (MQ=60)

                               GCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCC                     >  ERR1711319.621510/1‑251 (MQ=60)

                               GCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGAC                                                >  ERR1711319.490003/1‑224 (MQ=60)

                               GCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACC                                                                    >  ERR1711319.6852/1‑204 (MQ=60)

                                CACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCC                     <  ERR1711319.1024724/250‑1 (MQ=60)

                                    GAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTT                 >  ERR1711319.490764/1‑250 (MQ=60)

                                                 TCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGAT                                        <  ERR1711319.641829/214‑1 (MQ=60)

                                                 TCACCGGCCATGGCGTATTGAACT‑GGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCC  >  ERR1711319.834311/1‑251 (MQ=60)

                                                     CGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGCTTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACC                                             <  ERR1711319.25325/205‑1 (MQ=60)

CCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACCTCCGCCAACTGCAATTCGTGAACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTCCTTGGCCACTTGCCAGTTTTCAGCCC  >  AE015451/4741119‑4741418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`