Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I0 R1	216	53.7	1879433	97.4%	1830567	179.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	AE015451	4,808,177	Δ103 bp	45.7%	intergenic (‑286/‑167)	PP_4234 ← / → dsbD‑II	Aldehyde oxidase and xanthine dehydrogenase/thiol/disulfide interchange protein 2

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 4808176	2 (0.040)	5 (0.100)	5/322	NT	45.7%	intergenic (‑285/‑270)	PP_4234/dsbD‑II	Aldehyde oxidase and xanthine dehydrogenase/thiol/disulfide interchange protein 2
*	?	AE015451	4808280 =	10 (0.210)	5 (0.100)	5/322	NT	45.7%	intergenic (‑389/‑166)	PP_4234/dsbD‑II	Aldehyde oxidase and xanthine dehydrogenase/thiol/disulfide interchange protein 2

AGTCAAGCTGACAATGTGTCGCGGTGTGTATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/4808001‑4808176

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgccgcccCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCAGAACAAACTCTTACTACTCCTGACCCACCCCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACTCCTACT  >  AE015451/4808280‑4808480

AGTCAAGCTGACAATGTGTCGCGGTGTGTATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCA                                                                                                                                               <  1:732781/236‑1

                        TGTGTATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCAGAA                                                                                                                                            <  1:391742/215‑1

                                     ATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCCCTCAAAATTCCAACGCCG                                                                                                                                                                                        >  2:391739/1‑158

                                         GCGGAATTCGCGCTTTGGCGGGGAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGGTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCCCTCAAA                                                                                                                                                                                                    >  1:86447/1‑142

                                                                                GTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCAGAACAAACTCTT                                                                                                                                   <  2:156876/168‑1

                                                                                                                                                                             CCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCAGAACAAACTCTTACTACTCCTGACCCACCCCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACTCCTACT  <  1:788726/204‑1

                                                                                                                                                                             CCCCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCAGAACAAACTCTTACTACTCCTGACCCACCCCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACTCCTACT  >  2:788723/1‑204

AGTCAAGCTGACAATGTGTCGCGGTGTGTATAGGGCAATGGGCGGAATTCGCGCTTTGGCGGGTAGGTGTTTGGCGGGAGGTTTTCAGCGCCTGGGAGGTGTTCGCCGGGGGATTTTCAGCGCCTGGGAGGTGTTCGCCGGGAGAGTTTCAGCGCCTGGGAGATCGAGCGCCGCCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/4808001‑4808176

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgccgcccCCTCAAAATTCCAACGCCGCCTCCCCTCGAAAATCCAACAACAAAGACCCTGCAAACCCAGAACAAACTCTTACTACTCCTGACCCACCCCCAACACCCGCGTTAACCTTCATTTAATCAACCCCAGCCAGCATGGCCCCATTCCCAGATCTGCCAAGGCGAAGACATGCGCGTTCTCCTGCTCTTCCTCACACTCCTACT  >  AE015451/4808280‑4808480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A