Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I207 R1
|
218 |
45.0 |
2482936 |
97.1% |
2410930 |
86.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
1,941,604 |
A→C |
G10G (GGT→GGG) |
ruvC ← |
crossover junction endodeoxyribonuclease RuvC |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 1,941,604 | 0 | A | C | 100.0%
| 129.5
/ NA
| 38 | G10G (GGT→GGG) | ruvC | crossover junction endodeoxyribonuclease RuvC |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (15/23); total (15/23) |
TCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAACCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGACC > NZ_CP009273/1941518‑1941686
|
tcCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAcccc < 1:1046861/90‑1 (MQ=255)
ttGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCa > 2:266197/1‑90 (MQ=255)
tGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCaa < 2:426669/90‑1 (MQ=255)
gTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATg > 1:179480/1‑90 (MQ=255)
cGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg < 2:732089/90‑1 (MQ=255)
cGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg < 2:664557/90‑1 (MQ=255)
cGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg < 1:980198/90‑1 (MQ=255)
cGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg < 1:872957/90‑1 (MQ=255)
gATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCgag > 2:119098/1‑90 (MQ=255)
cATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGaata > 2:981804/1‑90 (MQ=255)
ggTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCAcgc < 2:452227/90‑1 (MQ=255)
gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGtc > 2:975339/1‑84 (MQ=255)
gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGtc < 1:975339/84‑1 (MQ=255)
gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGtt > 1:1049787/1‑90 (MQ=255)
gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGtt > 1:1159993/1‑90 (MQ=255)
gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGtt > 1:869974/1‑90 (MQ=255)
tGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGtttt < 1:449749/90‑1 (MQ=255)
tGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGtttt < 1:463394/90‑1 (MQ=255)
cctacctaCCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGc < 2:1069954/90‑1 (MQ=255)
acctacctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGt < 2:493844/90‑1 (MQ=255)
tacctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGAGTCTCCGTTTTGCTGTTTa > 1:704470/1‑90 (MQ=255)
cctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGc < 1:570328/90‑1 (MQ=255)
cctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGc < 1:444414/90‑1 (MQ=255)
tGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAg < 2:423518/90‑1 (MQ=255)
tGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAg < 2:869974/90‑1 (MQ=255)
tGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAg < 2:1190893/90‑1 (MQ=255)
gCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGc < 1:562338/90‑1 (MQ=255)
cGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGcc < 2:511365/90‑1 (MQ=255)
cGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGcc < 2:641568/90‑1 (MQ=255)
cGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGcc < 2:1049787/90‑1 (MQ=255)
ggATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCt > 1:149664/1‑90 (MQ=255)
tAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGt > 2:2226/1‑90 (MQ=255)
tAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGt < 1:742314/90‑1 (MQ=255)
ggTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTg > 2:480966/1‑90 (MQ=255)
ggTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTg > 2:1061150/1‑90 (MQ=255)
ggTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTg > 1:35929/1‑90 (MQ=255)
aCGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGAc > 1:18805/1‑90 (MQ=255)
cgcgcgACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGAcc < 2:18805/90‑1 (MQ=255)
cgcgcgACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGAcc < 2:749005/90‑1 (MQ=255)
|
TCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAACCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGACC > NZ_CP009273/1941518‑1941686
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
AAATCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAACCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGACCTCATCAGAGATTTCAC > NZ_CP009273/1941512‑1941702
|
AAATCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGAT < SRR3722089.563553/100‑1 (MQ=60)
ATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATG > SRR3722089.181725/1‑100 (MQ=60)
TCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGC < SRR3722089.1062549/100‑1 (MQ=60)
CGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAG < SRR3722089.994705/100‑1 (MQ=60)
CGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAG < SRR3722089.885494/100‑1 (MQ=60)
AGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTT > SRR3722089.1065524/1‑100 (MQ=60)
AGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTT > SRR3722089.882448/1‑100 (MQ=60)
AGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTT > SRR3722089.1177641/1‑100 (MQ=60)
AGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGC < SRR3722089.989746/100‑1 (MQ=60)
GTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGAGTCTCCGTTTTGCTGTTTA > SRR3722089.713648/1‑100 (MQ=60)
TGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGC < SRR3722089.455143/100‑1 (MQ=60)
TGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGC < SRR3722089.468927/100‑1 (MQ=60)
CCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCT > SRR3722089.151520/1‑100 (MQ=60)
CCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATC < SRR3722089.449747/100‑1 (MQ=60)
CCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATC < SRR3722089.577069/100‑1 (MQ=60)
GCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAG < SRR3722089.568919/100‑1 (MQ=60)
CGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTG > SRR3722089.36376/1‑100 (MQ=60)
GTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGAC > SRR3722089.19043/1‑100 (MQ=60)
TAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGACC < SRR3722089.752072/100‑1 (MQ=60)
ACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGACCTCATCAGAGATTTCAC > SRR3722089.500050/1‑100 (MQ=60)
ACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGACCTCATCAGAGATTTCAC > SRR3722089.146422/1‑100 (MQ=60)
ACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGACCTCATCAGAGATTTCAC > SRR3722089.1236710/1‑100 (MQ=60)
|
AAATCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAACCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGACCTCATCAGAGATTTCAC > NZ_CP009273/1941512‑1941702
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
