Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I207 R1
|
218 |
45.0 |
2482936 |
97.1% |
2410930 |
86.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
2,439,101 |
T→C |
K255E (AAG→GAG) |
mepA ← |
penicillin‑insensitive murein endopeptidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 2,439,101 | 0 | T | C | 100.0%
| 121.6
/ NA
| 36 | K255E (AAG→GAG) | mepA | penicillin‑insensitive murein endopeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (29/7); total (29/7) |
AAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTTCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATGGCGGTAAAG > NZ_CP009273/2439016‑2439184
|
aaaCAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCa < 2:526693/90‑1 (MQ=255)
ggCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCtt > 2:406126/1‑90 (MQ=255)
ggCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCtt > 2:125856/1‑90 (MQ=255)
ggCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCtt > 1:714785/1‑90 (MQ=255)
ggCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCtt > 2:1100608/1‑90 (MQ=255)
gCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCttt > 2:547115/1‑90 (MQ=255)
gCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCttt > 2:1137829/1‑90 (MQ=255)
tttCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCgg > 1:1134760/1‑90 (MQ=255)
tttCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCgg > 1:586547/1‑90 (MQ=255)
tttCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCgg > 2:1042403/1‑90 (MQ=255)
tCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCa < 1:145088/71‑1 (MQ=255)
tCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCa > 2:145088/1‑71 (MQ=255)
tCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGtt > 2:972359/1‑90 (MQ=255)
tCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGtt > 2:716611/1‑90 (MQ=255)
tCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGtt > 1:1222021/1‑90 (MQ=255)
tCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGtt > 1:184316/1‑90 (MQ=255)
tCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGGGTCTTCTCCTCAGGCTTTGTTGTTCCCGGtt > 1:865723/1‑90 (MQ=255)
tAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGa < 1:448337/90‑1 (MQ=255)
tAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGa < 2:184316/90‑1 (MQ=255)
aGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAg > 2:122215/1‑90 (MQ=255)
cGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCaa > 2:1049493/1‑90 (MQ=255)
aGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGc > 2:448261/1‑90 (MQ=255)
cgcCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTc > 1:943805/1‑90 (MQ=255)
gcCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGc > 1:794270/1‑84 (MQ=255)
gcCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGc < 2:794270/84‑1 (MQ=255)
gcCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCt > 2:947943/1‑90 (MQ=255)
gcCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCt > 2:423382/1‑90 (MQ=255)
gcCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCt > 1:844723/1‑90 (MQ=255)
tGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGcc > 1:373664/1‑90 (MQ=255)
tGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGcc > 2:88614/1‑90 (MQ=255)
gCAGGAAGGCAGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGcccc > 2:947164/1‑90 (MQ=255)
gAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCaa > 2:213156/1‑90 (MQ=255)
cggcAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATc > 1:1181212/1‑90 (MQ=255)
gAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATg < 1:406126/90‑1 (MQ=255)
ggCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATg > 1:321993/1‑88 (MQ=255)
ggCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATg < 2:321993/88‑1 (MQ=255)
tcttctcctCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATGGCGGTAAAg < 1:601627/90‑1 (MQ=255)
|
AAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTTCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATGGCGGTAAAG > NZ_CP009273/2439016‑2439184
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTTCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATGGCGGTAAAGGTTGATCTTCACAC > NZ_CP009273/2439003‑2439198
|
tcgtcggcagcgtcagatgtgtataagagacagCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCC < SRR3722089.925830/67‑1 (MQ=60)
AACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCT < SRR3722089.855017/100‑1 (MQ=60)
ACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTT > SRR3722089.724081/1‑100 (MQ=60)
aagagacagATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTG < SRR3722089.146891/91‑1 (MQ=60)
CTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGG > SRR3722089.1152020/1‑100 (MQ=60)
CTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGG > SRR3722089.593611/1‑100 (MQ=60)
ATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTT > SRR3722089.1240591/1‑100 (MQ=60)
ATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTT > SRR3722089.186617/1‑100 (MQ=60)
ATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGGGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTT > SRR3722089.878121/1‑100 (MQ=60)
CCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTctgtctcttatacacatctgacgctg > SRR3722089.378281/1‑74 (MQ=60)
TAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACC < SRR3722089.453719/100‑1 (MQ=60)
TGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGctgtctcttatacacatctgacgctgccgacgaccatcttag > SRR3722089.594233/1‑58 (MQ=60)
CATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTC > SRR3722089.957636/1‑100 (MQ=60)
ATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCT > SRR3722089.805213/1‑100 (MQ=60)
ATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCT > SRR3722089.856721/1‑100 (MQ=60)
CAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCC > SRR3722089.378246/1‑100 (MQ=60)
GGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATC > SRR3722089.1199206/1‑100 (MQ=60)
CGGCAACGGAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATGGC > SRR3722089.326052/1‑100 (MQ=60)
GAGGCGGTGTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATGGCGGTAAAGG < SRR3722089.411047/100‑1 (MQ=60)
GTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCtgtctcttatacacatctgacgctgccgacgacca > SRR3722089.1226527/1‑65 (MQ=60)
GTCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCctgtctcttatacacatctgacg > SRR3722089.733046/1‑77 (MQ=60)
TCTTCTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATGGCGGTAAAGGTTGATCTTC < SRR3722089.609039/100‑1 (MQ=60)
CTCCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATGGCGGTAAAGGTTGATCTTCACAC > SRR3722089.1152475/1‑100 (MQ=60)
|
GCGGGGAAACCATAAACAGGCTATTAAACGTTTCCATTAGATCACGTGCTCATCCAGTAGCGCCTGGCAGGAAGGCGGCAACGGAGGCGGTGTCTTCTTCTCAGGCTTTGTTGTTCCCGGTTTTGGAGGTTCAAACCAGCTTTGCAGTTCTGCCCCGCAACCATCGCCTGATGGCGGTAAAGGTTGATCTTCACAC > NZ_CP009273/2439003‑2439198
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
