Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I200 R1
|
216 |
30.8 |
1683622 |
97.2% |
1636480 |
87.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
463,972 |
A→G |
D45G (GAC→GGC) |
decR → |
DNA‑binding transcriptional regulator DecR |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 463,972 | 0 | A | G | 79.0%
| 29.7
/ 5.0
| 19 | D45G (GAC→GGC) | decR | DNA‑binding transcriptional regulator DecR |
| Reads supporting (aligned to +/- strand): ref base A (4/0); new base G (9/6); total (13/6) |
| Fisher's exact test for biased strand distribution p-value = 2.55e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 |
TTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAAC > NZ_CP009273/463886‑464058
|
ttGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCCCCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGAcggcgg < 1:13036/90‑1 (MQ=255)
ttGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGAcggcgg < 2:87531/90‑1 (MQ=255)
gCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTa > 1:431266/1‑90 (MQ=255)
ctctctTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCtt > 1:620017/1‑90 (MQ=255)
tctctTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTa > 2:820820/1‑90 (MQ=255)
gCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTc > 2:97861/1‑90 (MQ=255)
gCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTc > 1:552196/1‑90 (MQ=255)
gCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTc > 1:598490/1‑90 (MQ=255)
tGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGccc > 2:806909/1‑90 (MQ=255)
gTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCg > 1:720750/1‑90 (MQ=255)
aaTCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGACCCGGaa < 1:806909/90‑1 (MQ=255)
aCAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATa < 2:254972/90‑1 (MQ=255)
aCAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATa < 1:585241/90‑1 (MQ=255)
ccaccCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGcc > 2:614677/1‑90 (MQ=255)
accCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTc > 2:95652/1‑90 (MQ=255)
ttGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCt > 1:346812/1‑90 (MQ=255)
tGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAcc < 2:552196/90‑1 (MQ=255)
tGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAcc < 1:482650/90‑1 (MQ=255)
aaGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAcc > 2:533122/1‑87 (MQ=255)
aaGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGAcc < 1:533122/87‑1 (MQ=255)
gcCTGAAACGGCTGGAGGACGACGGGATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTtg > 2:743803/1‑90 (MQ=255)
acggcgGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAAc < 2:370481/90‑1 (MQ=255)
|
TTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAAC > NZ_CP009273/463886‑464058
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAAC > NZ_CP009273/463874‑464058
|
ACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGC < SRR3722079.96226/100‑1 (MQ=60)
CAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTA > SRR3722079.434999/1‑100 (MQ=60)
GCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATC > SRR3722079.824010/1‑100 (MQ=60)
GGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTT > SRR3722079.626321/1‑100 (MQ=60)
TTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCCCCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATC < SRR3722079.13130/100‑1 (MQ=60)
CTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTC > SRR3722079.557492/1‑100 (MQ=60)
CTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTC > SRR3722079.604498/1‑100 (MQ=60)
GGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCG > SRR3722079.728450/1‑100 (MQ=60)
AATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGACCCGGAAAAAATAGGCC < SRR3722079.815566/100‑1 (MQ=60)
ACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCC < SRR3722079.591048/100‑1 (MQ=60)
CAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCT > SRR3722079.349552/1‑100 (MQ=60)
TGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGC < SRR3722079.486867/100‑1 (MQ=60)
TGGAAGCGCCTGAAACGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGC < SRR3722079.538107/100‑1 (MQ=60)
CGGCTGGAGGACGGCGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAAC > SRR3722079.497396/1‑100 (MQ=60)
|
ACTGCAGCAGGATTGCACCCTCTCTTTGCAGGCACTGGCTGAAGCCGTTAATCTGACAACCACCCCTTGCTGGAAGCGCCTGAAACGGCTGGAGGACGACGGTATCCTTATCGGCAAAGTCGCCCTGCTGGATCCGGAAAAAATAGGCCTCGGCCTGACCGCTTTTGTGCTGATAAAAACGCAAC > NZ_CP009273/463874‑464058
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
