Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I210 R1
|
226 |
19.6 |
1130256 |
95.4% |
1078264 |
84.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
3,535,339 |
T→C |
F525S (TTC→TCC) |
feoB → |
Fe(2+) transporter permease subunit FeoB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 3,535,339 | 0 | T | C | 100.0%
| 56.7
/ NA
| 18 | F525S (TTC→TCC) | feoB | Fe(2+) transporter permease subunit FeoB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/4); total (14/4) |
CGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTG > NZ_CP009273/3535253‑3535424
|
cGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTCCCt < 1:424033/90‑1 (MQ=255)
aaaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAgcg > 2:78153/1‑90 (MQ=255)
ggCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGa > 2:37778/1‑90 (MQ=255)
gAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAt < 1:344444/90‑1 (MQ=255)
gAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAt < 1:395841/90‑1 (MQ=255)
ggCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAAtcgtc > 2:512438/1‑90 (MQ=255)
ttCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGAt < 1:380260/90‑1 (MQ=255)
aGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCt > 2:509683/1‑90 (MQ=255)
gATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGc > 1:14200/1‑90 (MQ=255)
gATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGc > 1:91607/1‑90 (MQ=255)
tcatcatcGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgt > 1:323183/1‑90 (MQ=255)
tcatcGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccg > 1:348409/1‑90 (MQ=255)
tcatcGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccg > 1:527026/1‑90 (MQ=255)
gTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGc > 1:196050/1‑90 (MQ=255)
cAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCg > 1:49876/1‑90 (MQ=255)
cAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCg > 1:126597/1‑90 (MQ=255)
gCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCggg > 1:270152/1‑90 (MQ=255)
aTTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTg > 2:343802/1‑90 (MQ=255)
|
CGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTG > NZ_CP009273/3535253‑3535424
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
ATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGG > NZ_CP009273/3535240‑3535422
|
ATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTC > SRR3722092.142951/1‑100 (MQ=60)
ATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTC > SRR3722092.41048/1‑100 (MQ=60)
ACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGC < SRR3722092.511235/100‑1 (MQ=60)
CGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTC < SRR3722092.432247/100‑1 (MQ=60)
gatctacactaagatggtcgtcggcagcgtcagatgtgtataagagacagGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTT < SRR3722092.175851/50‑1 (MQ=60)
GAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAAC < SRR3722092.403422/100‑1 (MQ=60)
GAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAAC < SRR3722092.350630/100‑1 (MQ=60)
TTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACG < SRR3722092.387470/100‑1 (MQ=60)
CTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGC > SRR3722092.93176/1‑100 (MQ=60)
CTGGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGC > SRR3722092.14443/1‑100 (MQ=60)
GGTAAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGT > SRR3722092.328912/1‑100 (MQ=60)
AAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCG > SRR3722092.354699/1‑100 (MQ=60)
AAAGTGATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCG > SRR3722092.537590/1‑100 (MQ=60)
GATCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGC > SRR3722092.199299/1‑100 (MQ=60)
TCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCG > SRR3722092.50720/1‑100 (MQ=60)
TCATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCG > SRR3722092.128739/1‑100 (MQ=60)
ATCATCGTCAGCATTTCCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGG > SRR3722092.274683/1‑100 (MQ=60)
|
ATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGG > NZ_CP009273/3535240‑3535422
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
