Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I195 R1
|
222 |
27.1 |
1551108 |
96.0% |
1489063 |
86.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
436,666 |
A→C |
L45R (CTG→CGG) |
xseB ← |
exodeoxyribonuclease VII small subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 436,666 | 0 | A | C | 100.0%
| 66.8
/ NA
| 20 | L45R (CTG→CGG) | xseB | exodeoxyribonuclease VII small subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (13/7); total (13/7) |
GGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTC > NZ_CP009273/436581‑436751
|
ggTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACATGCCCGCTg > 2:747449/1‑90 (MQ=255)
gCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCCGCTGCACGCCGCGt > 1:242683/1‑90 (MQ=255)
gcagAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCCGCTGCAc < 1:572036/64‑1 (MQ=255)
gcagAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCCGCTGCAc > 2:572036/1‑64 (MQ=255)
gcagAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCct > 1:240787/1‑90 (MQ=255)
tGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGc > 1:430816/1‑90 (MQ=255)
tACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCgg > 1:370687/1‑90 (MQ=255)
tgttgtAATTTGGCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTcc > 2:286444/1‑90 (MQ=255)
ttgtAATTTGGCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCCCTTTCCAg > 2:756299/1‑90 (MQ=255)
gtAATTTGGCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGAc < 2:242683/90‑1 (MQ=255)
tAATTTGGCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACg < 2:331288/90‑1 (MQ=255)
tAATTTGGCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACg < 2:413999/90‑1 (MQ=255)
tAATTTGGCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACg < 1:286444/90‑1 (MQ=255)
ggCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGGTAc > 1:728503/1‑90 (MQ=255)
ggCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGGCGCCACTTTCCAGACGGGTTAc > 1:415992/1‑90 (MQ=255)
gCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACa > 2:573940/1‑90 (MQ=255)
tGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATc < 2:370687/90‑1 (MQ=255)
tGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATc > 2:590038/1‑90 (MQ=255)
tGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAgctc < 2:668646/90‑1 (MQ=255)
tGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAgctc > 2:761597/1‑90 (MQ=255)
|
GGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATCTGTTCCAGCTC > NZ_CP009273/436581‑436751
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATC > NZ_CP009273/436574‑436740
|
TAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCCGCTGCAC < SRR3722073.417597/100‑1 (MQ=60)
GGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCCGCTGCACGCCGCGT > SRR3722073.247773/1‑100 (MQ=60)
tgtgtataagagacagTTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCG < SRR3722073.583987/84‑1 (MQ=60)
TTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCT > SRR3722073.245842/1‑100 (MQ=60)
CAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGC > SRR3722073.440036/1‑100 (MQ=60)
GCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGG > SRR3722073.378538/1‑100 (MQ=60)
GTTGTAATTTGGCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGGTAC > SRR3722073.743197/1‑100 (MQ=60)
GTTGTAATTTGGCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGGCGCCACTTTCCAGACGGGTTAC > SRR3722073.424881/1‑100 (MQ=60)
TAATTTGGCCTGCCCCTGACGTGCCCGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATC < SRR3722073.292510/100‑1 (MQ=60)
|
TAAAAGGGGTTAGAGAGGCGTCTTCATTGTCAGACAGCAGAATTTGTACGCGCTGTTCGGCTTGTTGTAATTTGGCCTGCCCCTGACGTGCCAGCTGCACGCCGCGTTCGAACTCGTTCAGCGCCTCTTCCAGCGGCAGGTCGCCACTTTCCAGACGGGTTACAATC > NZ_CP009273/436574‑436740
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
