Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I211 R1
|
223 |
18.0 |
1007644 |
96.3% |
970361 |
85.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
2,882,413 |
A→C |
V349G (GTT→GGT) |
cysI ← |
assimilatory sulfite reductase (NADPH) hemoprotein subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 2,882,413 | 0 | A | C | 100.0%
| 39.8
/ NA
| 13 | V349G (GTT→GGT) | cysI | assimilatory sulfite reductase (NADPH) hemoprotein subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (6/7); total (6/7) |
CCGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACT > NZ_CP009273/2882326‑2882493
|
ccGGTTTTCAGCGGACGCGCTGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAccc < 1:393112/90‑1 (MQ=255)
ccGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAccc < 1:284940/90‑1 (MQ=255)
ccGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAccc > 2:127123/1‑90 (MQ=255)
cGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAcccc < 1:435628/90‑1 (MQ=255)
gTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAg < 2:217722/90‑1 (MQ=255)
gACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGAt > 2:334575/1‑90 (MQ=255)
tCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTg < 1:484732/90‑1 (MQ=255)
cAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGa < 2:343472/90‑1 (MQ=255)
aGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAAc < 2:440711/90‑1 (MQ=255)
gCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCata < 1:156379/90‑1 (MQ=255)
gCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCata > 2:196911/1‑90 (MQ=255)
cGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCatat > 2:244940/1‑90 (MQ=255)
tCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATc < 1:196911/90‑1 (MQ=255)
tCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATc < 2:34564/90‑1 (MQ=255)
tGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATcccc > 1:248959/1‑90 (MQ=255)
ccAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCcgc > 2:84762/1‑90 (MQ=255)
aTGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACt > 2:292659/1‑90 (MQ=255)
|
CCGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACT > NZ_CP009273/2882326‑2882493
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CTCCAGCAGGCCGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACG > NZ_CP009273/2882316‑2882506
|
CTCCAGCAGGCCGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCC < SRR3722094.309457/100‑1 (MQ=60)
CTCCAGCAGGCCGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCC < SRR3722094.495656/100‑1 (MQ=60)
CCGGTTTTCAGCGGACGCGCTGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATAC < SRR3722094.399874/100‑1 (MQ=60)
CCGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATAC < SRR3722094.289225/100‑1 (MQ=60)
CGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACG < SRR3722094.443239/100‑1 (MQ=60)
TCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATG < SRR3722094.493283/100‑1 (MQ=60)
GCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATC < SRR3722094.158564/100‑1 (MQ=60)
TCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATT < SRR3722094.199604/100‑1 (MQ=60)
CAGCGTCAGGTGCCAGTTATCATCAATGCCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCC > SRR3722094.252576/1‑100 (MQ=60)
CCCTTACCCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACG > SRR3722094.385015/1‑100 (MQ=60)
|
CTCCAGCAGGCCGGTTTTCAGCGGACGCGCCGGATAATCAAGGATGCGACCATTTTCGATAAACAGCGTCAGGTGCCAGTTATCATCAATGCCCTTAACCCAGCCAATACGATCGCCTCGTCCGGTGAACTCATATGGACGGATCGGTTCAAATTTGATCCCCGCGCGACGCTCCACTTCCGCTTTAAACG > NZ_CP009273/2882316‑2882506
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
