Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F2 I208 R1 222 13.1 731276 96.6% 706412 85.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,021,663 (A)5→4 coding (1737/2055 nt) helD → DNA helicase IV

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,021,6590A.100.0% 21.0 / NA 6coding (1733/2055 nt)helDDNA helicase IV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (4/2);  total (4/2)

GGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATC  >  NZ_CP009273/1021576‑1021744
                                                                                   |                                                                                     
ggATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGaaaagcg                                                                                 >  1:18060/1‑87 (MQ=255)
     aGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAac                                                                           >  1:224413/1‑90 (MQ=255)
                                                ggCGCGTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCg                                                    <  1:254849/70‑1 (MQ=255)
                                                ggCGCGTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCg                                                    >  2:254849/1‑70 (MQ=255)
                                                                    ggCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGa            >  2:320623/1‑90 (MQ=255)
                                                                       cTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTa         >  2:238387/1‑90 (MQ=255)
                                                                              ccTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGtcatc  <  2:124017/90‑1 (MQ=255)
                                                                                   |                                                                                     
GGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATC  >  NZ_CP009273/1021576‑1021744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTA  >  NZ_CP009273/1021566‑1021737
                                                                                                 |                                                                          
ACGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCG                                                                         >  SRR3722090.18259/1‑100 (MQ=60)
     TTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAAC                                                                    >  SRR3722090.226971/1‑100 (MQ=60)
                                      taagagacagTTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGAC                                   <  SRR3722090.257965/90‑1 (MQ=60)
                                                                       CACATGAGGCCTGCCAGCCTGG‑AAAAGCGGCAACACGCTGGCCctgtctcttatacacaactccgagcccacgagactcctgagcatctcgtatgccgtc  >  SRR3722090.331657/1‑43 (MQ=60)
                                                                                                 |                                                                          
ACGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAAGAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAACACGCTGGCCGAAGTTGCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTA  >  NZ_CP009273/1021566‑1021737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑