Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F2 I208 R1
|
222 |
13.1 |
731276 |
96.6% |
706412 |
85.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NZ_CP009273 |
3,318,468 |
A→C |
A609A (GCT→GCG) |
ftsH ← |
ATP‑dependent zinc metalloprotease FtsH |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NZ_CP009273 | 3,318,468 | 0 | A | C | 100.0%
| 52.5
/ NA
| 16 | A609A (GCT→GCG) | ftsH | ATP‑dependent zinc metalloprotease FtsH |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (9/7); total (9/7) |
GGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGAAGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTGCGTCGATAGTCTCAT > NZ_CP009273/3318387‑3318553
|
ggTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTgg > 1:59990/1‑90 (MQ=255)
tgtTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGtt < 1:291770/90‑1 (MQ=255)
tACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGttctt > 1:287853/1‑90 (MQ=255)
ggTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGc > 1:219485/1‑90 (MQ=255)
cgGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACg < 1:291594/90‑1 (MQ=255)
cGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACg > 1:249745/1‑90 (MQ=255)
cGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACg < 2:68555/90‑1 (MQ=255)
tACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCt > 1:141517/1‑90 (MQ=255)
tACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCt > 1:211774/1‑90 (MQ=255)
tACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCt > 1:357772/1‑90 (MQ=255)
tACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCt > 2:26500/1‑90 (MQ=255)
tGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTg > 1:325320/1‑90 (MQ=255)
gTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTGc < 2:232082/90‑1 (MQ=255)
aGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTGCGTCGAt < 2:325320/90‑1 (MQ=255)
gTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTGCGTCGATAGtctc < 2:91275/90‑1 (MQ=255)
tAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTGCGTCGATAGTCTCat < 2:156913/90‑1 (MQ=255)
|
GGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGAAGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTGCGTCGATAGTCTCAT > NZ_CP009273/3318387‑3318553
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGAAGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTG > NZ_CP009273/3318369‑3318538
|
GCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGAC > SRR3722090.230237/1‑100 (MQ=60)
GCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGG > SRR3722090.60638/1‑100 (MQ=60)
GACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTT > SRR3722090.291457/1‑100 (MQ=60)
GTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGC > SRR3722090.221956/1‑100 (MQ=60)
TGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCC < SRR3722090.295430/100‑1 (MQ=60)
CGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGA < SRR3722090.295250/100‑1 (MQ=60)
GTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACG > SRR3722090.252768/1‑100 (MQ=60)
GCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCT > SRR3722090.142992/1‑100 (MQ=60)
GCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCT > SRR3722090.214135/1‑100 (MQ=60)
GCCTTTGGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCT > SRR3722090.362431/1‑100 (MQ=60)
GGACTACCATTGTCGCCAGAATTGTTAGACGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTG > SRR3722090.329541/1‑100 (MQ=60)
|
GCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTTAGAAGCGCCTGGTTCTTCCCAGCCCGCTGGCGGACGTACATCGCGACGTGCCATCAGGTCATCAATCTGCGGTG > NZ_CP009273/3318369‑3318538
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
