Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F2 I218 R1 11 14.7 995888 96.1% 957048 85.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,776,510 A→G L171P (CTG→CCG)  gpsA ← NAD(P)H‑dependent glycerol‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,776,5100AG100.0% 25.0 / NA 9L171P (CTG→CCG) gpsANAD(P)H‑dependent glycerol‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/4);  total (5/4)

TGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTG  >  NZ_CP009273/3776424‑3776579
                                                                                      |                                                                     
tGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTgcggca                                                                    <  2:383356/90‑1 (MQ=255)
    aaTAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGGAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCGGCAgctg                                                                >  2:127720/1‑90 (MQ=255)
        aCGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCGGCAGCTGCTgg                                                            >  2:71729/1‑90 (MQ=255)
                   cgcgCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCGGCAGCTGCTGGAGATCATCGGc                                                 <  2:179681/90‑1 (MQ=255)
                     cgccgcCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCGGCAGCTGCTGGAGATCATCGGCaa                                               <  2:47601/90‑1 (MQ=255)
                                            aaaTCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCGGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGc                        <  1:192369/90‑1 (MQ=255)
                                            aaaTCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCGGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGc                        <  2:231017/90‑1 (MQ=255)
                                                       gCTGTAAACGCGGAAACTTTTGCCGCAGTGCGGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGt             >  2:399520/1‑90 (MQ=255)
                                                            aaaCGCGGAAACTTTTGCCGCAGTGCGGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTa        >  2:268144/1‑90 (MQ=255)
                                                                  ggAAACTTTTGCCGCAGTGCGGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTg  >  1:325351/1‑90 (MQ=255)
                                                                                      |                                                                     
TGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTG  >  NZ_CP009273/3776424‑3776579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAACGTTG  >  NZ_CP009273/3776412‑3776603
                                                                                                  |                                                                                             
TCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCGG                                                                                              >  SRR3722101.150019/1‑100 (MQ=60)
   CCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCGGCAG                                                                                           <  SRR3722101.111605/100‑1 (MQ=60)
     GCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCGGCAGCT                                                                                         <  SRR3722101.276139/100‑1 (MQ=60)
                                                        AAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCGGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTG                                      <  SRR3722101.195178/100‑1 (MQ=60)
                                                                    CTGTAAACGCGGAAACTTTTGCCGCAGTGCGGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTG                          >  SRR3722101.330939/1‑100 (MQ=60)
                                                                                            CAGTGCGGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCtgtctcttatacacatctgacgctgccgacgaatgtcaatgtgtagatc  >  SRR3722101.193045/1‑51 (MQ=60)
                                                                                                  |                                                                                             
TCCCCGCACCAATGGCAATAACGTTTTTCACCGCGCCGCCAAGCTGCACGCCAATGAAATCCGGATTGCTGTAAACGCGGAAACTTTTGCCGCAGTGCAGCAGCTGCTGGAGATCATCGGCAAAGGTCTGATCGGTCGAGGCCAGCGAAATAGCTGTCGGTAAACCTGCCGCCAGTTCTTTCGCAAACGTTG  >  NZ_CP009273/3776412‑3776603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑