Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 531,155 G→A 36.9% intergenic (+2/‑11) gcl → / → hyi glyoxylate carboligase/hydroxypyruvate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273531,1550GA36.9% 272.5 / 268.7 242intergenic (+2/‑11)gcl/hyiglyoxylate carboligase/hydroxypyruvate isomerase
Reads supporting (aligned to +/- strand):  ref base G (56/96);  new base A (46/43);  total (102/140)
Fisher's exact test for biased strand distribution p-value = 3.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01

Only 100 of 252 total aligned reads displayed.

TCGGGTACCGGTAGTCGTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAAC  >  CP009273/531006‑531304
                                                                                                                                                     |                                                                                                                                                     
tCGGGTACCGGTAGTCGTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAgg                                                                                                                                                       <  2:552406/150‑1 (MQ=255)
tCGGGTACCGGTAGTCGTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAgg                                                                                                                                                       <  2:1130707/150‑1 (MQ=255)
  gggTACCGGTAGTCGTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGga                                                                                                                                                     <  2:3029293/150‑1 (MQ=255)
  gggTACCGGTAGTCGTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGga                                                                                                                                                     <  1:935701/150‑1 (MQ=255)
  gggTACCGGTAGTCGTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGga                                                                                                                                                     >  2:1713339/1‑150 (MQ=255)
   ggTACCGGTAGTCGTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGgag                                                                                                                                                    <  1:3104563/150‑1 (MQ=255)
       ccGGTAGTCGTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGataa                                                                                                                                                >  2:466997/1‑150 (MQ=255)
        cGGTAGTCGTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGataaa                                                                                                                                               >  1:3904021/1‑150 (MQ=255)
         ggTAGTCGTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGataaat                                                                                                                                              <  2:223267/150‑1 (MQ=255)
         ggTAGTCGTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGataaat                                                                                                                                              >  1:2367926/1‑150 (MQ=255)
            aGTCGTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAaataat                                                                                                                                           >  1:636070/1‑150 (MQ=255)
             gTCGTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATg                                                                                                                                          <  2:4060498/150‑1 (MQ=255)
             gTCGTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATg                                                                                                                                          <  2:3631544/150‑1 (MQ=255)
             gTCGTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATg                                                                                                                                          <  2:825771/150‑1 (MQ=255)
              tCGTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGt                                                                                                                                         <  1:1230796/150‑1 (MQ=255)
               cGTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGtt                                                                                                                                        <  1:120059/150‑1 (MQ=255)
                gTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTa                                                                                                                                       >  1:4128040/1‑150 (MQ=255)
                  ggAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACg                                                                                                                                     <  2:1060948/150‑1 (MQ=255)
                  ggAAGTTAGTCTCGAGCGAGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTGGAAGATATCGCCGATAACGAAGCGGACGCACCGAATGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACg                                                                                                                                     <  2:2478538/150‑1 (MQ=255)
                   gAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGt                                                                                                                                    <  2:238408/150‑1 (MQ=255)
                   gAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGt                                                                                                                                    >  2:4572400/1‑150 (MQ=255)
                    aaGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGtt                                                                                                                                   >  2:1928303/1‑150 (MQ=255)
                     aGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGttt                                                                                                                                  <  2:491357/150‑1 (MQ=255)
                       ttATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTtct                                                                                                                                <  2:3395257/150‑1 (MQ=255)
                        tATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTtctc                                                                                                                               >  1:4097866/1‑150 (MQ=255)
                         aTTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTctct                                                                                                                              >  2:2422079/1‑150 (MQ=255)
                            ctcGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCt                                                                                                                           >  1:1841144/1‑150 (MQ=255)
                                aGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAAtt                                                                                                                       >  2:3600111/1‑150 (MQ=255)
                                aGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAAtt                                                                                                                       >  2:1174858/1‑150 (MQ=255)
                                aGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAAtt                                                                                                                       <  2:770547/150‑1 (MQ=255)
                                  cGTGTGACCAATATTTCGATGGGCAGCGAACTGTATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTa                                                                                                                     <  1:1408363/150‑1 (MQ=255)
                                  cGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTa                                                                                                                     <  2:1821443/150‑1 (MQ=255)
                                  cGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTa                                                                                                                     >  2:1501309/1‑150 (MQ=255)
                                     gtgACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCg                                                                                                                  >  2:4096386/1‑150 (MQ=255)
                                       gACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGAt                                                                                                                >  2:3149556/1‑150 (MQ=255)
                                       gACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGAt                                                                                                                <  1:2693722/150‑1 (MQ=255)
                                        aCCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATg                                                                                                               <  1:1021841/150‑1 (MQ=255)
                                          cAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGATATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGtt                                                                                                             <  1:3687631/150‑1 (MQ=255)
                                          cAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGtt                                                                                                             >  2:3833517/1‑150 (MQ=255)
                                           aaTATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTa                                                                                                            >  1:405464/1‑150 (MQ=255)
                                             tatTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTAtt                                                                                                          <  2:401064/150‑1 (MQ=255)
                                                  cGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGgag                                                                                                     <  2:4515831/150‑1 (MQ=255)
                                                   gATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGgaga                                                                                                    <  1:2593643/150‑1 (MQ=255)
                                                      gggCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAata                                                                                                 <  2:68004/150‑1 (MQ=255)
                                                          aGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGAt                                                                                             >  1:1951502/1‑150 (MQ=255)
                                                          aGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGAt                                                                                             >  2:2600729/1‑150 (MQ=255)
                                                            cGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGAttt                                                                                           <  2:1805073/150‑1 (MQ=255)
                                                             gAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGAtttt                                                                                          <  2:3193744/150‑1 (MQ=255)
                                                              aaCTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTtc                                                                                         >  1:4582122/1‑150 (MQ=255)
                                                                 tGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCg                                                                                      >  2:2624524/1‑150 (MQ=255)
                                                                   gATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGcc                                                                                    <  2:76668/150‑1 (MQ=255)
                                                                   gATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACAATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGcc                                                                                    >  2:1985033/1‑150 (MQ=255)
                                                                       aCGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGtt                                                                                <  1:4120039/150‑1 (MQ=255)
                                                                       aCGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGtt                                                                                <  1:21388/150‑1 (MQ=255)
                                                                            aTGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTgag                                                                           >  2:733196/1‑150 (MQ=255)
                                                                             tGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATGTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTgaga                                                                          >  1:3328724/1‑150 (MQ=255)
                                                                               gAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGATATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGaaa                                                                        <  1:785788/150‑1 (MQ=255)
                                                                               gAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGaaa                                                                        >  2:4490890/1‑150 (MQ=255)
                                                                                   ttGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCGGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTg                                                                    <  1:2179430/150‑1 (MQ=255)
                                                                                   ttGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTg                                                                    >  1:3789201/1‑150 (MQ=255)
                                                                                    tGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTgc                                                                   >  2:3857384/1‑150 (MQ=255)
                                                                                     gAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTgcg                                                                  <  1:1713339/150‑1 (MQ=255)
                                                                                     gAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTgcg                                                                  >  1:3518906/1‑150 (MQ=255)
                                                                                         atatCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAAGAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAgt                                                              <  2:1606109/150‑1 (MQ=255)
                                                                                            tCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGTGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGtgtg                                                           <  1:1490271/150‑1 (MQ=255)
                                                                                             cGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTgg                                                          <  2:2800266/150‑1 (MQ=255)
                                                                                              gCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGt                                                         <  1:321233/150‑1 (MQ=255)
                                                                                                   tAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTATTGAGAAAGCTGCGCAGTGTGGTTTTcg                                                    >  2:1198034/1‑150 (MQ=255)
                                                                                                    aaCGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTcgc                                                   <  1:1387862/150‑1 (MQ=255)
                                                                                                     aCGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTcgcg                                                  <  2:2147046/150‑1 (MQ=255)
                                                                                                     aCGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTcgcg                                                  <  1:1130322/150‑1 (MQ=255)
                                                                                                       gCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCgcggc                                                <  2:266693/150‑1 (MQ=255)
                                                                                                         aGCGGACGCACCGACTGAAACCTGCTTCATNCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTATTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGt                                              >  1:4282263/1‑150 (MQ=255)
                                                                                                            ggACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGa                                           <  1:1739627/150‑1 (MQ=255)
                                                                                                              aCGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAAt                                         <  2:1916784/150‑1 (MQ=255)
                                                                                                                gCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAAttt                                       <  2:3672061/150‑1 (MQ=255)
                                                                                                                     gACTGAAACCTTCTTCATGCACTATGAATAATGGAGATAAATATTGTTACGTTTATCTGATAATTTATCTATGTTATTTGGATAATATGATTTTCTCTCCCGTTTTGAGAAAGCTTAGCAGTGTTGTTTTCGCGGCGTTGAATTTAtttt                                  <  2:2933744/150‑1 (MQ=255)
                                                                                                                      aCTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGttt                                 >  2:2072145/1‑150 (MQ=255)
                                                                                                                      aCTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGttt                                 <  2:4278503/150‑1 (MQ=255)
                                                                                                                      aCTGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGttt                                 >  1:2871626/1‑150 (MQ=255)
                                                                                                                        tGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTcc                               >  1:1463857/1‑150 (MQ=255)
                                                                                                                        tGAAACCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTcc                               >  1:3436579/1‑150 (MQ=255)
                                                                                                                          aaaCCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCtt                             <  2:1372193/150‑1 (MQ=255)
                                                                                                                          aaaCCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCtt                             <  2:3008493/150‑1 (MQ=255)
                                                                                                                          aaaCCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCtt                             <  2:1049802/150‑1 (MQ=255)
                                                                                                                           aaCCTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTa                            >  2:440866/1‑150 (MQ=255)
                                                                                                                             ccTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATg                          >  2:3312760/1‑150 (MQ=255)
                                                                                                                             ccTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATg                          >  2:3485434/1‑150 (MQ=255)
                                                                                                                             ccTGCTTCATGCACTATGAATAAGAGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATg                          <  1:4096386/150‑1 (MQ=255)
                                                                                                                                    cATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGa                   <  1:2528872/150‑1 (MQ=255)
                                                                                                                                        cACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACAtt               <  1:3886206/150‑1 (MQ=255)
                                                                                                                                        cACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACAtt               <  1:32014/150‑1 (MQ=255)
                                                                                                                                        cACTATGAATAAGAGAGATAAATAATGTTACGTGTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACAtt               <  2:1543681/150‑1 (MQ=255)
                                                                                                                                         aCTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTg              <  2:2327516/150‑1 (MQ=255)
                                                                                                                                          cTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTga             >  2:2001201/1‑150 (MQ=255)
                                                                                                                                             tGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTgaaga          <  1:3085007/150‑1 (MQ=255)
                                                                                                                                                 tAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTa      >  1:753077/1‑150 (MQ=255)
                                                                                                                                                   aGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTaaa    <  2:351710/150‑1 (MQ=255)
                                                                                                                                                     ggAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAAc  >  1:2774987/1‑150 (MQ=255)
                                                                                                                                                     ggAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAAc  <  2:3782338/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCGGGTACCGGTAGTCGTGGAAGTTATTCTCGAGCGTGTGACCAATATTTCGATGGGCAGCGAACTGGATAACGTCATGGAATTTGAAGATATCGCCGATAACGCAGCGGACGCACCGACTGAAACCTGCTTCATGCACTATGAATAAGGGAGATAAATAATGTTACGTTTCTCTGCTAATTTATCGATGTTATTTGGAGAATATGATTTTCTCGCCCGTTTTGAGAAAGCTGCGCAGTGTGGTTTTCGCGGCGTTGAATTTATGTTTCCTTATGACTACGACATTGAAGAATTAAAAC  >  CP009273/531006‑531304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A