Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,722,475 G→T 43.1% D128Y (GAC→TAC)  gloA → glyoxalase I, Ni‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,722,4750GT43.1% 287.6 / inf 280D128Y (GAC→TAC) gloAglyoxalase I, Ni‑dependent
Reads supporting (aligned to +/- strand):  ref base G (67/92);  new base T (63/58);  total (130/150)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 280 total aligned reads displayed.

GTAGATAACGCCGCTGAAGCGTGCGAAAAAATCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACT  >  CP009273/1722328‑1722623
                                                                                                                                                   |                                                                                                                                                    
gTAGATAACGCCGCTGAAGCGTGCGAAAAAATCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATAc                                                                                                                                                    <  1:3873315/150‑1 (MQ=255)
gTAGATAACGCCGCTGAAGCGTGCGAAAAAATCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATAc                                                                                                                                                    <  1:2114245/150‑1 (MQ=255)
 tAGATAACGCCGCTGAAGCGTGCGAAAAAATCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACg                                                                                                                                                   <  1:608525/150‑1 (MQ=255)
    aTAACGCCGCTGAAGCGTGCGAAAAAATCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCg                                                                                                                                                <  1:4403608/150‑1 (MQ=255)
    aTAACGCCGCTGAAGCGTGCGAAAAAATCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCg                                                                                                                                                <  1:3034408/150‑1 (MQ=255)
     tAACGCCGCTGAAGCGTGCGAAAAAATCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCgg                                                                                                                                               <  1:4631274/150‑1 (MQ=255)
     tAACGCCGCTGAAGCGTGCGAAAAAATCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCgg                                                                                                                                               <  2:3267009/150‑1 (MQ=255)
      aaCGCCGCTGAAGCGTGCGAAAAAATCCGTCAAAAGGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGATGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGt                                                                                                                                              <  2:3549101/150‑1 (MQ=255)
      aaCGCCGCTGAAGCGTGCGAAAAAATCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGt                                                                                                                                              >  1:3296320/1‑150 (MQ=255)
           cgcTGAAGCGTGCGAAAAAATCCGTCAACACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCgg                                                                                                                                         <  2:438949/150‑1 (MQ=255)
             cTGAAGCGTGCGAAAAAATCCGTCAAAACGGGGGTAACGTGACCCGTGACGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGCAGAGAAATACTCCGGTCGCGGTc                                                                                                                                       >  1:2255377/1‑150 (MQ=255)
              tGAAGCGTGCGAAAAAATCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGTGTCCGGAAAAAGGCGGTACAACGGTTAACTCGATTGTGGACGATCCGGACGGTTACAAAATAGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCt                                                                                                                                      >  1:2909214/1‑150 (MQ=255)
               gAAGCGTGCGAAAAAATCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTg                                                                                                                                     >  1:1819139/1‑150 (MQ=255)
                   cGTGCGAAAAAATCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCa                                                                                                                                 <  1:2092260/150‑1 (MQ=255)
                     tGCGAAAAAATCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAAc                                                                                                                               >  1:2973762/1‑150 (MQ=255)
                          aaaaaTCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAAtc                                                                                                                          >  1:1034756/1‑150 (MQ=255)
                           aaaaTCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAAtct                                                                                                                         >  2:554650/1‑150 (MQ=255)
                             aaTCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTcc                                                                                                                       >  2:110373/1‑150 (MQ=255)
                              aTCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCt                                                                                                                      <  2:1116202/150‑1 (MQ=255)
                              aTCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCt                                                                                                                      <  2:1116137/150‑1 (MQ=255)
                                ccGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGc                                                                                                                    <  2:2885377/150‑1 (MQ=255)
                                 cGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGcc                                                                                                                   <  1:2010404/150‑1 (MQ=255)
                                 cGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGcc                                                                                                                   <  1:3822995/150‑1 (MQ=255)
                                  gTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCg                                                                                                                  >  2:1898443/1‑150 (MQ=255)
                                     aaaaCGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGc                                                                                                               <  2:2413251/150‑1 (MQ=255)
                                     aaaaCGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGc                                                                                                               >  1:2432551/1‑150 (MQ=255)
                                       aaCGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGt                                                                                                             >  1:3376475/1‑150 (MQ=255)
                                        aCGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTg                                                                                                            <  2:3157039/150‑1 (MQ=255)
                                        aCGGGGGTAAAGTGACCAGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACTGTTACAAATTTGAGTTAATCGAAGAGTACGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTg                                                                                                            <  2:4461290/150‑1 (MQ=255)
                                         cGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGa                                                                                                           >  2:4271553/1‑150 (MQ=255)
                                          gggggTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGaa                                                                                                          >  1:1182261/1‑150 (MQ=255)
                                          gggggTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGaa                                                                                                          >  1:1725020/1‑150 (MQ=255)
                                          gggggTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGaa                                                                                                          >  1:4152039/1‑150 (MQ=255)
                                          gggggTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGaa                                                                                                          >  2:1055728/1‑150 (MQ=255)
                                            gggTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACt                                                                                                        <  2:4441203/150‑1 (MQ=255)
                                               tAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCAt                                                                                                     >  2:1971150/1‑150 (MQ=255)
                                               tAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCAt                                                                                                     >  1:3234811/1‑150 (MQ=255)
                                               tAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCAt                                                                                                     >  1:3234958/1‑150 (MQ=255)
                                                aaCGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATc                                                                                                    >  2:803692/1‑150 (MQ=255)
                                                aaCGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATc                                                                                                    >  2:3754406/1‑150 (MQ=255)
                                                aaCGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATc                                                                                                    <  2:3080472/150‑1 (MQ=255)
                                                 aCGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATcg                                                                                                   >  2:4548412/1‑150 (MQ=255)
                                                    tGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCgcgc                                                                                                >  2:3003737/1‑150 (MQ=255)
                                                    tGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCgcgc                                                                                                >  2:3003645/1‑150 (MQ=255)
                                                     gACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGcc                                                                                               >  2:492445/1‑150 (MQ=255)
                                                      aCCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGccc                                                                                              <  1:451203/150‑1 (MQ=255)
                                                      aCCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGccc                                                                                              >  2:1696969/1‑150 (MQ=255)
                                                      aCCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGccc                                                                                              <  1:2054321/150‑1 (MQ=255)
                                                       cccGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCg                                                                                             >  2:2165069/1‑150 (MQ=255)
                                                        ccGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGc                                                                                            >  2:2446211/1‑150 (MQ=255)
                                                           tGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATc                                                                                         >  2:4393027/1‑150 (MQ=255)
                                                           tGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATc                                                                                         <  2:27224/150‑1 (MQ=255)
                                                            gAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACACAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCt                                                                                        <  2:608436/150‑1 (MQ=255)
                                                              aGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCttt                                                                                      <  2:2355134/150‑1 (MQ=255)
                                                              aGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCAGGGCGTGAACTCATCGCGCCCGCATCttt                                                                                      >  2:729113/1‑150 (MQ=255)
                                                              aGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCAGGGCGTGAACTCATCGCGCCCGCATCttt                                                                                      >  2:2968554/1‑150 (MQ=255)
                                                                cGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTAc                                                                                    >  1:1441577/1‑150 (MQ=255)
                                                                     ccGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCAt                                                                               <  2:2917838/150‑1 (MQ=255)
                                                                     ccGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCAt                                                                               <  2:2267031/150‑1 (MQ=255)
                                                                       ggTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCg                                                                             <  1:2729104/150‑1 (MQ=255)
                                                                         tAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGAc                                                                           <  1:4619369/150‑1 (MQ=255)
                                                                             aGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGt                                                                       <  2:3972372/150‑1 (MQ=255)
                                                                              ggCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTa                                                                      >  2:3880852/1‑150 (MQ=255)
                                                                              ggCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTa                                                                      <  1:4321694/150‑1 (MQ=255)
                                                                                 ggTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAata                                                                   >  2:2670498/1‑150 (MQ=255)
                                                                                  gTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAatat                                                                  >  1:3563199/1‑150 (MQ=255)
                                                                                    actacGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATAttt                                                                <  1:2477421/150‑1 (MQ=255)
                                                                                     ctacGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTg                                                               <  2:1898270/150‑1 (MQ=255)
                                                                                       acGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTc                                                             <  1:3942931/150‑1 (MQ=255)
                                                                                       acGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTc                                                             >  2:3346265/1‑150 (MQ=255)
                                                                                        cGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCa                                                            <  1:3144721/150‑1 (MQ=255)
                                                                                        cGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCa                                                            <  1:3470978/150‑1 (MQ=255)
                                                                                         ggTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCAt                                                           >  1:4054778/1‑150 (MQ=255)
                                                                                         ggTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCAt                                                           <  1:310437/150‑1 (MQ=255)
                                                                                           ttATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATaa                                                         <  2:1610220/150‑1 (MQ=255)
                                                                                            tATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAAt                                                        <  1:1533988/150‑1 (MQ=255)
                                                                                                gcgTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATgcgc                                                    <  1:3976654/150‑1 (MQ=255)
                                                                                                   tttGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCt                                                 <  2:1957893/150‑1 (MQ=255)
                                                                                                   tttGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCt                                                 <  2:2828803/150‑1 (MQ=255)
                                                                                                   tttGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCt                                                 >  2:3628931/1‑150 (MQ=255)
                                                                                                      gtgGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCa                                              >  1:4125959/1‑150 (MQ=255)
                                                                                                       tgGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCaa                                             >  2:231898/1‑150 (MQ=255)
                                                                                                            gATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTAt                                        >  1:4599212/1‑150 (MQ=255)
                                                                                                              tCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATcc                                      >  2:561934/1‑150 (MQ=255)
                                                                                                              tCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATcc                                      <  1:1712014/150‑1 (MQ=255)
                                                                                                                 ggACGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTa                                   >  1:4383495/1‑150 (MQ=255)
                                                                                                                   aCGGTTACAAAATTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTAtt                                 <  1:688369/150‑1 (MQ=255)
                                                                                                                        tACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAagag                            <  1:641185/150‑1 (MQ=255)
                                                                                                                             aaTTGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCa                       <  1:499744/150‑1 (MQ=255)
                                                                                                                                tGAGTTAATCGAAGAGAAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGAt                    >  2:1547327/1‑150 (MQ=255)
                                                                                                                                tGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGAt                    <  2:1838153/150‑1 (MQ=255)
                                                                                                                                   gTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTc                 <  1:1627165/150‑1 (MQ=255)
                                                                                                                                   gTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTc                 <  1:3194503/150‑1 (MQ=255)
                                                                                                                                   gTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTc                 >  2:3501662/1‑150 (MQ=255)
                                                                                                                                       aTCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGAt             <  1:539468/150‑1 (MQ=255)
                                                                                                                                         cGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATaa           <  2:1275084/150‑1 (MQ=255)
                                                                                                                                             gagaAATACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCt       >  1:495541/1‑150 (MQ=255)
                                                                                                                                                aaaGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCaa    >  2:4105815/1‑150 (MQ=255)
                                                                                                                                                  aGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACt  <  2:1242390/150‑1 (MQ=255)
                                                                                                                                                  aGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACt  <  2:506990/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTAGATAACGCCGCTGAAGCGTGCGAAAAAATCCGTCAAAACGGGGGTAACGTGACCCGTGAAGCGGGTCCGGTAAAAGGCGGTACTACGGTTATCGCGTTTGTGGAAGATCCGGACGGTTACAAAATTGAGTTAATCGAAGAGAAAGACGCCGGTCGCGGTCTGGGCAACTAATCTCCTGCCGGGCGTGAACTCATCGCGCCCGCATCTTTACTGCATCGACAAGTAATATTTGTCATAATGCGCGCTGCAATTTATCCGTATTAAGAGAATCAGATGTCCGATAACGCTCAACT  >  CP009273/1722328‑1722623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A