Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F224 I0 R1	1457	284.0	9290224	95.7%	8890744	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,727,929	C→A	37.4%	S1529* (TCA→TAA)	lhr →	putative ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,727,929	0	C	A	37.4%	432.2 / 288.8	285	S1529* (TCA→TAA)	lhr	putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand): ref base C (83/95); new base A (48/59); total (131/154)
Fisher's exact test for biased strand distribution p-value = 8.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

AAAAGAGGAATTACTCGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTC  >  CP009273/1727780‑1728076

aaaaGAGGAATTACTCGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTc                                                                                                                                                     <  2:66064/150‑1 (MQ=255)

aaaaGAGGAATTACTCGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTc                                                                                                                                                     >  1:1767671/1‑150 (MQ=255)

   agagGAATTACTCGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAg                                                                                                                                                  >  2:4108994/1‑150 (MQ=255)

    gagGAATTACTCGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGt                                                                                                                                                 >  1:2418752/1‑150 (MQ=255)

    gagGAATTACTCGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGt                                                                                                                                                 >  2:1447923/1‑150 (MQ=255)

      ggAATTACTCGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTc                                                                                                                                               <  2:3664486/150‑1 (MQ=255)

       gAATTACTCGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCg                                                                                                                                              <  2:1907954/150‑1 (MQ=255)

          ttACTCGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCca                                                                                                                                           <  2:782150/150‑1 (MQ=255)

           tACTCGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCcac                                                                                                                                          <  1:2020802/150‑1 (MQ=255)

             cTCGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACaa                                                                                                                                        >  1:3469693/1‑150 (MQ=255)

              tCGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAg                                                                                                                                       >  1:1420636/1‑150 (MQ=255)

              tCGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAg                                                                                                                                       <  2:2752868/150‑1 (MQ=255)

              tCGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAg                                                                                                                                       <  2:1544479/150‑1 (MQ=255)

              tCGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAg                                                                                                                                       <  2:3220369/150‑1 (MQ=255)

               cGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAgg                                                                                                                                      <  2:3608842/150‑1 (MQ=255)

               cGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAgg                                                                                                                                      >  1:2612519/1‑150 (MQ=255)

               cGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAgg                                                                                                                                      >  2:3561440/1‑150 (MQ=255)

                      gagGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGAt                                                                                                                               >  2:890219/1‑150 (MQ=255)

                       agGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGAtt                                                                                                                              <  1:3664249/150‑1 (MQ=255)

                         gTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTgg                                                                                                                            <  1:3110813/150‑1 (MQ=255)

                         gTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTgg                                                                                                                            >  1:2914087/1‑150 (MQ=255)

                          ttttCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTggg                                                                                                                           <  2:1042564/150‑1 (MQ=255)

                             tCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATATTCAAGTTCGCCACAAGGGCTGGATTGGGGAt                                                                                                                        <  1:3852813/150‑1 (MQ=255)

                                aCGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATaga                                                                                                                     <  1:713807/150‑1 (MQ=255)

                                 cgcgCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATagag                                                                                                                    >  2:1302801/1‑150 (MQ=255)

                                   cgcTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGaa                                                                                                                  >  1:1956563/1‑150 (MQ=255)

                                     cTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAg                                                                                                                <  2:2429385/150‑1 (MQ=255)

                                     cTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAg                                                                                                                <  2:2653171/150‑1 (MQ=255)

                                      tGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAgg                                                                                                               >  1:2584188/1‑150 (MQ=255)

                                      tGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAgg                                                                                                               <  2:645447/150‑1 (MQ=255)

                                      tGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAgg                                                                                                               >  1:4520538/1‑150 (MQ=255)

                                       gACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGa                                                                                                              >  2:907825/1‑150 (MQ=255)

                                         cTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACATAAGAGAATGATCTACCGTTCCGGCAAACGCCGATGTATACGCTGCAGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACt                                                                                                            >  1:4246482/1‑150 (MQ=255)

                                             cGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACg                                                                                                        <  2:2451778/150‑1 (MQ=255)

                                                  tGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGc                                                                                                   >  1:3282313/1‑150 (MQ=255)

                                                   gCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGcc                                                                                                  <  2:4009771/150‑1 (MQ=255)

                                                    cGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGccc                                                                                                 <  1:3492493/150‑1 (MQ=255)

                                                     gTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCg                                                                                                >  2:204963/1‑150 (MQ=255)

                                                       cgcgAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGtt                                                                                              >  1:3323506/1‑150 (MQ=255)

                                                        gcgAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTc                                                                                             >  2:212306/1‑150 (MQ=255)

                                                            aCCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCAt                                                                                         <  1:1672191/150‑1 (MQ=255)

                                                              cACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATcc                                                                                       >  1:3716113/1‑150 (MQ=255)

                                                                    tGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGt                                                                                 <  1:899150/150‑1 (MQ=255)

                                                                      cgcTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTAt                                                                               <  2:4073059/150‑1 (MQ=255)

                                                                        cTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTg                                                                             <  1:2088865/150‑1 (MQ=255)

                                                                        cTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTg                                                                             <  2:4212132/150‑1 (MQ=255)

                                                                        cTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTg                                                                             >  1:96167/1‑150 (MQ=255)

                                                                        cTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTg                                                                             >  2:4379702/1‑150 (MQ=255)

                                                                          ttACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCa                                                                           <  2:3942695/150‑1 (MQ=255)

                                                                           tACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAg                                                                          <  1:1405604/150‑1 (MQ=255)

                                                                           tACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAg                                                                          <  1:2969950/150‑1 (MQ=255)

                                                                           tACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAg                                                                          <  2:3162799/150‑1 (MQ=255)

                                                                              gCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGAc                                                                       <  1:946064/150‑1 (MQ=255)

                                                                               cTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACg                                                                      >  2:1354371/1‑150 (MQ=255)

                                                                                tAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACgg                                                                     >  2:1349256/1‑150 (MQ=255)

                                                                                tAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACgg                                                                     >  2:3414782/1‑150 (MQ=255)

                                                                                 aaCAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGa                                                                    <  1:2329525/150‑1 (MQ=255)

                                                                                  aCAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGAGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGat                                                                   <  1:1367651/150‑1 (MQ=255)

                                                                                    aGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGatta                                                                 >  2:185695/1‑150 (MQ=255)

                                                                                    aGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGatta                                                                 >  2:2073259/1‑150 (MQ=255)

                                                                                     gAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGattat                                                                <  2:3833822/150‑1 (MQ=255)

                                                                                           aaTGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCcgcg                                                          <  1:3303220/150‑1 (MQ=255)

                                                                                           aaTGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCcgcg                                                          <  2:1744076/150‑1 (MQ=255)

                                                                                           aaTGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCcgcg                                                          <  1:1166606/150‑1 (MQ=255)

                                                                                           aaTGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCcgcg                                                          >  2:2618570/1‑150 (MQ=255)

                                                                                            aTGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGt                                                         >  2:2886222/1‑150 (MQ=255)

                                                                                              gATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTcc                                                       >  2:4507157/1‑150 (MQ=255)

                                                                                                 cTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGc                                                    <  1:2842621/150‑1 (MQ=255)

                                                                                                 cTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGc                                                    <  1:3566677/150‑1 (MQ=255)

                                                                                                 cTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGc                                                    <  1:3566455/150‑1 (MQ=255)

                                                                                                           ggCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACt                                          >  1:1074911/1‑150 (MQ=255)

                                                                                                             cAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTg                                        <  2:2969097/150‑1 (MQ=255)

                                                                                                              aaaCGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGt                                       >  2:4563848/1‑150 (MQ=255)

                                                                                                              aaaCGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGt                                       >  1:4139236/1‑150 (MQ=255)

                                                                                                               aaCGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTa                                      >  2:1770957/1‑150 (MQ=255)

                                                                                                                 cGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTAtt                                    <  1:191711/150‑1 (MQ=255)

                                                                                                                  gCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTAttt                                   <  1:1741516/150‑1 (MQ=255)

                                                                                                                  gCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTAttt                                   <  2:521999/150‑1 (MQ=255)

                                                                                                                   ccGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTa                                  <  2:4170604/150‑1 (MQ=255)

                                                                                                                    cGATGTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAg                                 >  1:4134000/1‑150 (MQ=255)

                                                                                                                     gATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGc                                <  2:3903856/150‑1 (MQ=255)

                                                                                                                        gTTTACGCTGCTGCGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGc                             <  1:4043152/150‑1 (MQ=255)

                                                                                                                             cGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGttt                        <  1:767201/150‑1 (MQ=255)

                                                                                                                                tgctgcGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCtt                     >  1:1885052/1‑150 (MQ=255)

                                                                                                                                 gctgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCttt                    <  1:60048/150‑1 (MQ=255)

                                                                                                                                 gctgcGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGTTTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCttt                    <  2:332285/150‑1 (MQ=255)

                                                                                                                                   tgcGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGa                  >  1:2148900/1‑150 (MQ=255)

                                                                                                                                    gcGTGAGGCGGGGTTTTAAAGTTCGCCACAAGGGGTGGAGTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTGAGACTTGTAGTTAGCGGCCGTTTCTTTgtt                 >  2:1618310/1‑148 (MQ=255)

                                                                                                                                    gcGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCGTTGAt                 >  2:113325/1‑150 (MQ=255)

                                                                                                                                     cGTGAGGCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTATTCAGACTTGTATTTAGCGGCAGTTTCTTTGATc                <  2:1405951/150‑1 (MQ=255)

                                                                                                                                       tGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAg              <  2:3796784/150‑1 (MQ=255)

                                                                                                                                        gAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCATCAGACTTGTATTTAGCGGCAGTTTCTTTTATAAgc             >  1:3174759/1‑150 (MQ=255)

                                                                                                                                         aGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAgct            >  2:3878044/1‑150 (MQ=255)

                                                                                                                                         aGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAgct            >  1:1595239/1‑150 (MQ=255)

                                                                                                                                         aGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTGGTATTTAGCGGCAGTTTCTTTGATCAgct            >  1:3979525/1‑150 (MQ=255)

                                                                                                                                          ggCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAgctg           <  1:470030/150‑1 (MQ=255)

                                                                                                                                           gCGGGATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACTGATGCCCGTTCTCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAgctgc          <  2:2186387/150‑1 (MQ=255)

                                                                                                                                              ggATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCtgctgc       <  2:2576710/150‑1 (MQ=255)

                                                                                                                                              ggATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCtgctgc       >  2:2017933/1‑150 (MQ=255)

                                                                                                                                               gATTTTAAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCa      <  1:2351350/150‑1 (MQ=255)

AAAAGAGGAATTACTCGCCCCAGAGGTTTTCCACGCGCTGACTACCGCACTGCGTCGCGAACCACGGCTGCGCTTTACGCTAACAGAAGTGAATGATCTACCGGTCCGGCAAACGCCGATGTTTACGCTGCTGCGTGAGGCGGGATTTTCAAGTTCGCCACAAGGGCTGGATTGGGGATAGAGAAAGGACTGACGGATGCCCGTTCGCATCCGTCAGTATTGCAGGACGGATTATTCCGCGTCCGGCTCTTCAGACTTGTATTTAGCGGCAGTTTCTTTGATCAGCTGCTGCAGTTC  >  CP009273/1727780‑1728076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A