Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,732,093 G→T 34.0% intergenic (‑291/‑8) ynhF ← / → purR stress response membrane/transcriptional repressor, hypoxanthine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,732,0930GT34.0% 409.5 / 224.6 241intergenic (‑291/‑8)ynhF/purRstress response membrane/transcriptional repressor, hypoxanthine‑binding
Reads supporting (aligned to +/- strand):  ref base G (87/72);  new base T (50/32);  total (137/104)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

Only 100 of 244 total aligned reads displayed.

CTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTA  >  CP009273/1731944‑1732242
                                                                                                                                                     |                                                                                                                                                     
 ttACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGa                                                                                                                                                      <  2:4309648/150‑1 (MQ=255)
  tACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGaa                                                                                                                                                     <  1:3470067/150‑1 (MQ=255)
    cacTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAaat                                                                                                                                                   >  1:2961733/1‑150 (MQ=255)
    cacTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAaat                                                                                                                                                   <  1:4426405/150‑1 (MQ=255)
     acTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTCTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAaatg                                                                                                                                                  >  2:2196575/1‑150 (MQ=255)
         tttGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAaatggaat                                                                                                                                              <  1:4103808/150‑1 (MQ=255)
         tttGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAaatggaat                                                                                                                                              <  1:3339437/150‑1 (MQ=255)
              gTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGcaa                                                                                                                                         >  2:558544/1‑150 (MQ=255)
               tACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGcaac                                                                                                                                        <  1:3374112/150‑1 (MQ=255)
               tACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGcaac                                                                                                                                        >  1:4205399/1‑150 (MQ=255)
                aCTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGcaaca                                                                                                                                       <  1:3484695/150‑1 (MQ=255)
                 cTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGcaacaa                                                                                                                                      >  1:1838170/1‑150 (MQ=255)
                 cTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGcaacaa                                                                                                                                      >  1:2544958/1‑150 (MQ=255)
                     ccATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATaaa                                                                                                                                  <  2:4166972/150‑1 (MQ=255)
                        ttGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGa                                                                                                                               >  1:1479730/1‑150 (MQ=255)
                          gACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATg                                                                                                                             >  1:4109572/1‑150 (MQ=255)
                          gACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATg                                                                                                                             >  1:54351/1‑150 (MQ=255)
                           aCCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGt                                                                                                                            <  1:1086289/150‑1 (MQ=255)
                               cTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCg                                                                                                                        <  2:3852546/150‑1 (MQ=255)
                               cTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCg                                                                                                                        <  2:3203321/150‑1 (MQ=255)
                                ttCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGa                                                                                                                       <  1:4618843/150‑1 (MQ=255)
                                  ccTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGaaa                                                                                                                     >  1:1302935/1‑150 (MQ=255)
                                   cTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAAc                                                                                                                    >  1:3107442/1‑150 (MQ=255)
                                   cTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAAc                                                                                                                    <  2:742709/150‑1 (MQ=255)
                                       cGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGc                                                                                                                >  2:839690/1‑150 (MQ=255)
                                       cGCTCCGTGTCGTTTTTACGGCGTACCGAAACAATTTTGTTGTGCGTAAGGTTTGTAAAGGCAAACGTTTACCTTTCGATTTTGCAGTAGATTAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAAAAAAATATTTAGCGAAACGatc                                                                                                                >  1:3189353/1‑148 (MQ=255)
                                        gCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCa                                                                                                               >  2:4450096/1‑150 (MQ=255)
                                         cTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCaa                                                                                                              <  2:1772931/150‑1 (MQ=255)
                                          tCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCaaa                                                                                                             >  1:288270/1‑150 (MQ=255)
                                              tgtCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGtt                                                                                                         >  1:886557/1‑150 (MQ=255)
                                              tgtCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGtt                                                                                                         >  1:2397021/1‑150 (MQ=255)
                                              tgtCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGtt                                                                                                         >  1:645888/1‑150 (MQ=255)
                                                tCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTc                                                                                                       <  2:43199/150‑1 (MQ=255)
                                                 cGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTcc                                                                                                      >  1:1527718/1‑150 (MQ=255)
                                                  gTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCa                                                                                                     <  1:417501/150‑1 (MQ=255)
                                                       tCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACa                                                                                                <  2:1197313/150‑1 (MQ=255)
                                                            cGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACtgt                                                                                           >  2:2137227/1‑150 (MQ=255)
                                                            cGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACtgt                                                                                           >  2:4514860/1‑150 (MQ=255)
                                                              tACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTgtgt                                                                                         >  1:4012964/1‑150 (MQ=255)
                                                                ccGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTca                                                                                       <  1:2986956/150‑1 (MQ=255)
                                                                  gCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTcaca                                                                                     >  2:3496342/1‑150 (MQ=255)
                                                                      cacTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTg                                                                                 >  1:1128619/1‑150 (MQ=255)
                                                                       acTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGa                                                                                >  2:3160045/1‑150 (MQ=255)
                                                                        cTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGAt                                                                               >  2:2079267/1‑150 (MQ=255)
                                                                        cTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGAt                                                                               >  2:618079/1‑150 (MQ=255)
                                                                          tttGTTGTGCGTATGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATca                                                                             <  1:495888/150‑1 (MQ=255)
                                                                          tttGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATca                                                                             >  2:1333163/1‑150 (MQ=255)
                                                                           ttgttgTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATcaa                                                                            <  1:2624403/150‑1 (MQ=255)
                                                                                gtgCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACaaaa                                                                       >  2:3145680/1‑150 (MQ=255)
                                                                                    gTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACg                                                                   >  1:478989/1‑150 (MQ=255)
                                                                                    gTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACg                                                                   <  1:2098194/150‑1 (MQ=255)
                                                                                     tAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGt                                                                  >  1:3504993/1‑150 (MQ=255)
                                                                                      aaGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGtt                                                                 >  1:744312/1‑150 (MQ=255)
                                                                                       aGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGttt                                                                <  1:962995/150‑1 (MQ=255)
                                                                                       aGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGttt                                                                <  1:962618/150‑1 (MQ=255)
                                                                                        ggTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTtc                                                               >  2:4204919/1‑150 (MQ=255)
                                                                                        ggTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTtc                                                               <  2:870187/150‑1 (MQ=255)
                                                                                          tgtgtAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTtcgt                                                             >  2:253989/1‑150 (MQ=255)
                                                                                            tgtAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTtcgtcg                                                           <  2:2936801/150‑1 (MQ=255)
                                                                                              tAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCt                                                         >  1:775651/1‑150 (MQ=255)
                                                                                               aaaGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTg                                                        >  1:35689/1‑150 (MQ=255)
                                                                                               aaaGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTg                                                        >  2:1747200/1‑150 (MQ=255)
                                                                                               aaaGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTg                                                        >  2:2587572/1‑150 (MQ=255)
                                                                                                 aGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTgaa                                                      >  2:1929004/1‑150 (MQ=255)
                                                                                                 aGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTgaa                                                      >  2:1929170/1‑150 (MQ=255)
                                                                                                 aGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTgaa                                                      >  2:3931500/1‑150 (MQ=255)
                                                                                                   gCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTgaaga                                                    >  1:541681/1‑150 (MQ=255)
                                                                                                     aaaCGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGaaa                                                  >  2:1231664/1‑150 (MQ=255)
                                                                                                      aaCGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAAc                                                 <  1:1750752/150‑1 (MQ=255)
                                                                                                      aaCGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAAc                                                 >  1:2020999/1‑150 (MQ=255)
                                                                                                         gTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAAcgcg                                              >  1:1658154/1‑150 (MQ=255)
                                                                                                           ttACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCa                                            >  2:903918/1‑150 (MQ=255)
                                                                                                            tACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCaa                                           >  2:722213/1‑150 (MQ=255)
                                                                                                            tACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCaa                                           >  2:4577793/1‑150 (MQ=255)
                                                                                                               cTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGc                                        >  2:1659749/1‑150 (MQ=255)
                                                                                                                 tGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCg                                      >  2:2762423/1‑150 (MQ=255)
                                                                                                                   cGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCgtg                                    <  1:3348698/150‑1 (MQ=255)
                                                                                                                   cGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCgtg                                    <  1:3023008/150‑1 (MQ=255)
                                                                                                                    gATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCgtgt                                   <  2:2596576/150‑1 (MQ=255)
                                                                                                                    gATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCgtgt                                   <  2:2961708/150‑1 (MQ=255)
                                                                                                                     aTTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGtgtg                                  >  1:3683399/1‑150 (MQ=255)
                                                                                                                     aTTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGtgtg                                  <  2:1101668/150‑1 (MQ=255)
                                                                                                                         tGCAGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCa                              <  1:1707683/150‑1 (MQ=255)
                                                                                                                           cAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGc                            >  2:477078/1‑150 (MQ=255)
                                                                                                                           cAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGc                            >  1:4397095/1‑150 (MQ=255)
                                                                                                                            aGGAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCg                           >  2:2044866/1‑150 (MQ=255)
                                                                                                                              gAGCTGAAGTTAGGGTCTGGAGTTAAATGGAATGGCAACAATAACAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGAt                         <  2:815079/150‑1 (MQ=255)
                                                                                                                              gAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGAt                         <  2:1479730/150‑1 (MQ=255)
                                                                                                                               aGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACCATAAAAGATGTATCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGAtt                        <  2:3199508/150‑1 (MQ=255)
                                                                                                                                gCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTa                       >  1:3295304/1‑150 (MQ=255)
                                                                                                                                        tAGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTa               >  1:1289174/1‑150 (MQ=255)
                                                                                                                                         aGGGTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTac              >  1:1011575/1‑150 (MQ=255)
                                                                                                                                            gTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACact           >  2:2704306/1‑150 (MQ=255)
                                                                                                                                            gTCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACact           >  2:3096370/1‑150 (MQ=255)
                                                                                                                                            gTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACact           <  2:540377/150‑1 (MQ=255)
                                                                                                                                             tCTGGAGTTAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACacta          <  1:768985/150‑1 (MQ=255)
                                                                                                                                               tGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACactact        <  1:375989/150‑1 (MQ=255)
                                                                                                                                                  aGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTccc     >  2:1553254/1‑150 (MQ=255)
                                                                                                                                                  aGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTccc     >  1:2458631/1‑150 (MQ=255)
                                                                                                                                                   gTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTcccc    >  1:2514523/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTACACTATTTGCGTACTGGCCATTGACCCCTTCCTGACGCTCCGTGTCGTTTTTCCGGCGTACCGCAACACTTTTGTTGTGCGTAAGGTGTGTAAAGGCAAACGTTTACCTTGCGATTTTGCAGGAGCTGAAGTTAGGGTCTGGAGTGAAATGGAATGGCAACAATAAAAGATGTAGCGAAACGAGCAAACGTTTCCACTACAACTGTGTCACACGTGATCAACAAAACACGTTTCGTCGCTGAAGAAACGCGCAACGCCGTGTGGGCAGCGATTAAAGAATTACACTACTCCCCTA  >  CP009273/1731944‑1732242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A