Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,822,975 C→A 14.7% A339A (GCG→GCT)  astD ← succinylglutamic semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,822,9750CA14.7% 887.4 / 82.9 264A339A (GCG→GCT) astDsuccinylglutamic semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (111/114);  new base A (27/12);  total (138/126)
Fisher's exact test for biased strand distribution p-value = 2.43e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

AAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTG  >  CP009273/1822826‑1823124
                                                                                                                                                     |                                                                                                                                                     
 aaGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCg                                                                                                                                                      >  2:3049166/1‑150 (MQ=255)
 aaGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCg                                                                                                                                                      >  2:3277597/1‑150 (MQ=255)
   gTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCt                                                                                                                                                    <  2:1572692/150‑1 (MQ=255)
   gTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCt                                                                                                                                                    <  2:4429118/150‑1 (MQ=255)
       cATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCa                                                                                                                                                >  1:4456197/1‑150 (MQ=255)
          aaCGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGtt                                                                                                                                             >  2:1077851/1‑150 (MQ=255)
          aaCGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGtt                                                                                                                                             >  1:2885571/1‑150 (MQ=255)
            cGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTtgc                                                                                                                                           <  2:2757961/150‑1 (MQ=255)
             gCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATAGCTTCCAGTtgct                                                                                                                                          <  2:547680/150‑1 (MQ=255)
                aGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATAGCTTCCAGTTGCTGcc                                                                                                                                       >  1:326997/1‑150 (MQ=255)
                   cgcgCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATg                                                                                                                                    <  2:2129426/150‑1 (MQ=255)
                     cgcAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCa                                                                                                                                  >  1:4241380/1‑150 (MQ=255)
                      gcAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAg                                                                                                                                 <  2:1273239/150‑1 (MQ=255)
                        aaTAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATAGCTTCCAGTTGCTGCCATGCAGTa                                                                                                                               >  1:1611109/1‑150 (MQ=255)
                         aTAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTaa                                                                                                                              >  1:3798450/1‑150 (MQ=255)
                              ggTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAaccacc                                                                                                                         >  1:3238133/1‑150 (MQ=255)
                               gTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACct                                                                                                                        <  1:368122/150‑1 (MQ=255)
                                tCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACctg                                                                                                                       >  2:2866516/1‑150 (MQ=255)
                                  cGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACctgct                                                                                                                     <  2:1060760/150‑1 (MQ=255)
                                  cGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACctgct                                                                                                                     <  1:2723733/150‑1 (MQ=255)
                                  cGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACctgct                                                                                                                     <  1:1767074/150‑1 (MQ=255)
                                    aaCACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCtgt                                                                                                                   >  1:4049456/1‑150 (MQ=255)
                                     acacCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCtgtg                                                                                                                  <  1:1019301/150‑1 (MQ=255)
                                          tctTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATAGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGcc                                                                                                             <  1:3971478/150‑1 (MQ=255)
                                              cATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGtt                                                                                                         >  1:41247/1‑150 (MQ=255)
                                              cATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGtt                                                                                                         >  2:1870096/1‑150 (MQ=255)
                                              cATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATAGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGtt                                                                                                         >  1:205722/1‑150 (MQ=255)
                                               aTCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTc                                                                                                        <  1:264378/150‑1 (MQ=255)
                                               aTCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTc                                                                                                        <  1:1255347/150‑1 (MQ=255)
                                                   ggTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGaa                                                                                                    >  1:3735204/1‑150 (MQ=255)
                                                     tACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAAt                                                                                                  >  1:2109560/1‑150 (MQ=255)
                                                     tACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAAt                                                                                                  >  2:4454237/1‑150 (MQ=255)
                                                       cGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCa                                                                                                <  1:1086552/150‑1 (MQ=255)
                                                       cGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCa                                                                                                <  1:2392677/150‑1 (MQ=255)
                                                            gCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCg                                                                                           <  2:2747235/150‑1 (MQ=255)
                                                            gCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCg                                                                                           <  2:3719548/150‑1 (MQ=255)
                                                              aaCGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGcc                                                                                         >  2:3680380/1‑150 (MQ=255)
                                                              aaCGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATAGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGcc                                                                                         >  2:4131642/1‑150 (MQ=255)
                                                              aaCGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATAGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGcc                                                                                         >  2:4010495/1‑150 (MQ=255)
                                                              aaCGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATAGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGcc                                                                                         >  2:3745169/1‑150 (MQ=255)
                                                              aaCGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATAGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGcc                                                                                         >  2:3631982/1‑150 (MQ=255)
                                                               aCGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCa                                                                                        >  2:432322/1‑150 (MQ=255)
                                                                   cTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATaa                                                                                    >  2:3157761/1‑150 (MQ=255)
                                                                    tGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATaaa                                                                                   >  2:591875/1‑150 (MQ=255)
                                                                     gTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAAc                                                                                  >  1:2174953/1‑150 (MQ=255)
                                                                       cATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACgg                                                                                <  1:250804/150‑1 (MQ=255)
                                                                         tttCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCt                                                                              <  2:2633308/150‑1 (MQ=255)
                                                                         tttCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCt                                                                              <  1:227826/150‑1 (MQ=255)
                                                                          ttCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTg                                                                             >  2:2572313/1‑150 (MQ=255)
                                                                          ttCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATAGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTg                                                                             >  1:3839396/1‑150 (MQ=255)
                                                                            cAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCg                                                                           >  2:2179219/1‑150 (MQ=255)
                                                                               tGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGtt                                                                        <  2:3944151/150‑1 (MQ=255)
                                                                               ggagccccGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCGTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGtt                                                                        <  2:922144/146‑1 (MQ=255)
                                                                                    cccGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCa                                                                   <  1:2628824/150‑1 (MQ=255)
                                                                                    cccGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCa                                                                   <  2:3805552/150‑1 (MQ=255)
                                                                                     ccGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATAGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCAt                                                                  >  2:192300/1‑150 (MQ=255)
                                                                                       ggCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATcc                                                                >  1:298818/1‑150 (MQ=255)
                                                                                        gCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATccc                                                               <  2:2501559/150‑1 (MQ=255)
                                                                                         cGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCa                                                              <  2:3531335/150‑1 (MQ=255)
                                                                                         cGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATAGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCa                                                              >  1:975401/1‑150 (MQ=255)
                                                                                          gTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAg                                                             <  2:2217509/150‑1 (MQ=255)
                                                                                           tCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGt                                                            <  1:3679619/150‑1 (MQ=255)
                                                                                            cAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGtt                                                           <  2:492661/150‑1 (MQ=255)
                                                                                            cAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGtt                                                           <  1:3069407/150‑1 (MQ=255)
                                                                                             aGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTg                                                          <  2:3438817/150‑1 (MQ=255)
                                                                                               cAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGcc                                                        >  2:579939/1‑150 (MQ=255)
                                                                                                aaCGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATAGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGccc                                                       >  2:2958219/1‑150 (MQ=255)
                                                                                                   gATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGc                                                    >  1:1288872/1‑150 (MQ=255)
                                                                                                        cccTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTaa                                               >  2:1829927/1‑150 (MQ=255)
                                                                                                         ncTGCTTGNAATAAGCNCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCANTAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAAt                                              >  2:646418/2‑150 (MQ=255)
                                                                                                         ccTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAAt                                              <  1:3524457/150‑1 (MQ=255)
                                                                                                         ccTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAAt                                              <  1:590123/150‑1 (MQ=255)
                                                                                                          cTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATc                                             >  1:3624660/1‑150 (MQ=255)
                                                                                                             cTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCgct                                          <  1:461366/150‑1 (MQ=255)
                                                                                                              ttGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATAGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCgctg                                         <  2:1181849/150‑1 (MQ=255)
                                                                                                                  aataaGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCgctggctg                                     >  2:4604458/1‑150 (MQ=255)
                                                                                                                   ataaGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGa                                    >  1:649866/1‑150 (MQ=255)
                                                                                                                   ataaGCGCGGCGCAAGCAGGGGTCGTCCGCCCATAGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGa                                    >  1:1345007/1‑150 (MQ=255)
                                                                                                                     aaGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACg                                  <  1:497392/150‑1 (MQ=255)
                                                                                                                     aaGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACg                                  >  2:1708116/1‑150 (MQ=255)
                                                                                                                       gcgcGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGc                                <  2:4456197/150‑1 (MQ=255)
                                                                                                                        cgcgGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAATGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCa                               <  1:765521/150‑1 (MQ=255)
                                                                                                                               cAAGCAGGGGTCGTCCGCCCATAGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGAc                        <  1:3993704/150‑1 (MQ=255)
                                                                                                                                 aGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGa                      >  1:2812300/1‑150 (MQ=255)
                                                                                                                                   cAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGc                    >  1:3011384/1‑150 (MQ=255)
                                                                                                                                    aGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCa                   >  1:3463690/1‑150 (MQ=255)
                                                                                                                                     ggggTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCaa                  <  1:2322235/150‑1 (MQ=255)
                                                                                                                                       ggTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGa                <  2:870568/150‑1 (MQ=255)
                                                                                                                                       ggTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGa                >  1:1957247/1‑150 (MQ=255)
                                                                                                                                           gtcCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATACACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACg            <  2:424507/150‑1 (MQ=255)
                                                                                                                                           gtcCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACg            >  1:2009820/1‑150 (MQ=255)
                                                                                                                                           gtcCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACg            >  1:3133137/1‑150 (MQ=255)
                                                                                                                                           gtcCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTCCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACg            >  1:2885172/1‑150 (MQ=255)
                                                                                                                                            accGCCCATCGCTACCAGTTGCTGAAATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGACAATAAACGGATGCGGTTCGTCATCCCAGATGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGc           >  2:1650816/2‑150 (MQ=255)
                                                                                                                                               gCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATc        <  2:3164207/150‑1 (MQ=255)
                                                                                                                                               gCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATc        <  1:365132/150‑1 (MQ=255)
                                                                                                                                                 ccATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGc      >  1:3236654/1‑150 (MQ=255)
                                                                                                                                                  cATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGcc     >  2:2378334/1‑150 (MQ=255)
                                                                                                                                                   aTCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGccc    >  1:1427752/1‑150 (MQ=255)
                                                                                                                                                     cGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTg  <  2:1309363/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAAGTATCATAACGCCAGACGCGCAATAACGGTCCGAACACCTCTTCATCTGGTACGCCAGCAACGCCTGTCATTTCAATGATCCCCGGCGTCAGCAACGATGTCCCTGCTTGTAATAAGCGCGGCGCAAGCAGGGGTCGTCCGCCCATCGCTTCCAGTTGCTGCCATGCAGTAACCACCTGCTGTGCGGCCTGTTCAGAAATCAGCCCGCCAATAAACGGCTGCGGTTCGTCATCCCAGTTGCCCGGCGTTAATCGCTGGCTGACGGCAACCAGACGAGCAAGAAACGCATCGCCCTG  >  CP009273/1822826‑1823124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A