Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,373,129 C→A 10.5% D332Y (GAT→TAT)  menF ← isochorismate synthase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,373,1290CA10.5% 1001.6 / 57.0 271D332Y (GAT→TAT) menFisochorismate synthase 2
Reads supporting (aligned to +/- strand):  ref base C (121/121);  new base A (15/14);  total (136/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

TTCGCTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCT  >  CP009273/2372980‑2373278
                                                                                                                                                     |                                                                                                                                                     
ttCGCTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCAcatc                                                                                                                                                       <  2:1616589/150‑1 (MQ=255)
 tCGCTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCAcatca                                                                                                                                                      >  2:3959212/1‑150 (MQ=255)
  cGCTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCAcatcat                                                                                                                                                     >  1:3852963/1‑150 (MQ=255)
  cGCTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCAcatcat                                                                                                                                                     >  2:470266/1‑150 (MQ=255)
  cGCTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCAcatcat                                                                                                                                                     >  1:2890026/1‑150 (MQ=255)
    cTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATcc                                                                                                                                                   <  2:3785358/150‑1 (MQ=255)
    cTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATcc                                                                                                                                                   <  2:3656330/150‑1 (MQ=255)
    cTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATcc                                                                                                                                                   >  2:3442598/1‑150 (MQ=255)
    cTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATcc                                                                                                                                                   <  1:4378132/150‑1 (MQ=255)
    cTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATcc                                                                                                                                                   <  1:2383461/150‑1 (MQ=255)
     tttGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCg                                                                                                                                                  >  2:1553895/1‑150 (MQ=255)
        gttgtAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCtt                                                                                                                                               >  2:4125731/1‑150 (MQ=255)
           gtAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTttgt                                                                                                                                            <  1:4305679/150‑1 (MQ=255)
              aTGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGa                                                                                                                                         >  2:3186547/1‑150 (MQ=255)
               tGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAg                                                                                                                                        <  2:3097925/150‑1 (MQ=255)
                gagagaTAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGt                                                                                                                                       >  1:4552172/1‑150 (MQ=255)
                  gagaTAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGa                                                                                                                                     <  1:3217720/150‑1 (MQ=255)
                   agaTAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGaa                                                                                                                                    >  1:3030246/1‑150 (MQ=255)
                    gaTAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTGTGTTGAGTGAAg                                                                                                                                   >  2:3974729/1‑150 (MQ=255)
                          ccGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGa                                                                                                                             <  1:4438298/150‑1 (MQ=255)
                                 aCCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAgcg                                                                                                                      >  2:3059750/1‑150 (MQ=255)
                                      cGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGaa                                                                                                                 <  2:813708/150‑1 (MQ=255)
                                      cGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGaa                                                                                                                 >  1:884332/1‑150 (MQ=255)
                                      cGTACCAGTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGaa                                                                                                                 <  2:480447/150‑1 (MQ=255)
                                       gTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAg                                                                                                                >  2:4068207/1‑150 (MQ=255)
                                         aCCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGAt                                                                                                              <  1:669916/150‑1 (MQ=255)
                                           cATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGATTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGAtgc                                                                                                            <  1:2672829/150‑1 (MQ=255)
                                             ttCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGAtgctg                                                                                                          <  1:1457819/150‑1 (MQ=255)
                                                tcGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCAc                                                                                                       >  2:2395731/1‑150 (MQ=255)
                                                tcGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCAc                                                                                                       <  2:1660588/150‑1 (MQ=255)
                                                 cGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACt                                                                                                      >  1:3740783/1‑150 (MQ=255)
                                                  gggTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACtt                                                                                                     <  1:1968823/150‑1 (MQ=255)
                                                   ggTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACttt                                                                                                    <  2:4539543/150‑1 (MQ=255)
                                                    gTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTa                                                                                                   <  1:1654694/150‑1 (MQ=255)
                                                     tGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTac                                                                                                  <  1:819928/150‑1 (MQ=255)
                                                     tGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTac                                                                                                  <  2:1904441/150‑1 (MQ=255)
                                                      gAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTacg                                                                                                 <  1:3249591/150‑1 (MQ=255)
                                                      gAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTacg                                                                                                 >  2:1156201/1‑150 (MQ=255)
                                                        aCGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTacgca                                                                                               >  2:634410/1‑150 (MQ=255)
                                                           gTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTacgcagac                                                                                            <  2:4010018/150‑1 (MQ=255)
                                                                 gACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTa                                                                                      <  1:1789938/150‑1 (MQ=255)
                                                                  aCGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTAc                                                                                     <  1:1587346/150‑1 (MQ=255)
                                                                  aCGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTAc                                                                                     <  1:2197471/150‑1 (MQ=255)
                                                                   cGGGCGATAATCTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGATGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTAcc                                                                                    <  1:3381735/150‑1 (MQ=255)
                                                                   cGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTAcc                                                                                    >  1:4218788/1‑150 (MQ=255)
                                                                        gATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTgcgg                                                                               >  2:3298264/1‑150 (MQ=255)
                                                                        gATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTgcgg                                                                               <  1:910229/150‑1 (MQ=255)
                                                                        gATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGATGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGAAACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTgcgg                                                                               >  2:3718206/1‑150 (MQ=255)
                                                                         aTAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTgcggc                                                                              >  1:2678235/1‑150 (MQ=255)
                                                                           aaaCTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGcggcgg                                                                            >  1:3101536/1‑150 (MQ=255)
                                                                            aaCTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGt                                                                           >  1:3277579/1‑150 (MQ=255)
                                                                            aaCTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGt                                                                           <  1:3578889/150‑1 (MQ=255)
                                                                             aCTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTa                                                                          <  2:3293239/150‑1 (MQ=255)
                                                                              cTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTaa                                                                         >  1:3908309/1‑150 (MQ=255)
                                                                              cTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTaa                                                                         >  1:3908210/1‑150 (MQ=255)
                                                                               tGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTaaa                                                                        >  1:1140292/1‑150 (MQ=255)
                                                                               tGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTaaa                                                                        >  1:4114638/1‑150 (MQ=255)
                                                                               tGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTaaa                                                                        >  2:4354948/1‑150 (MQ=255)
                                                                                  cgcgCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACa                                                                     >  1:243740/1‑150 (MQ=255)
                                                                                  cgcgCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACa                                                                     >  2:2845044/1‑150 (MQ=255)
                                                                                      ccAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCa                                                                 <  1:2012418/150‑1 (MQ=255)
                                                                                       cAGATCGCGCGGAAAGCCAGCAACTGCTGCCGTTTGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTCTTGAGTGAAGTCCAGATACAGAGGCGAAGATGCTGCACATTACGCAGACGCAGTACCTGCGGCCGTAAAACAACCAg                                                                >  2:880261/1‑150 (MQ=255)
                                                                                           tCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTc                                                            >  1:3687778/1‑150 (MQ=255)
                                                                                                    aaGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCg                                                   <  2:4031137/150‑1 (MQ=255)
                                                                                                    aaGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCg                                                   >  2:2171830/1‑150 (MQ=255)
                                                                                                    aaGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCg                                                   <  1:169707/150‑1 (MQ=255)
                                                                                                    aaGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCg                                                   <  2:3651565/150‑1 (MQ=255)
                                                                                                          gCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCGGGGTATCGGCCTGt                                             >  2:4429347/1‑150 (MQ=255)
                                                                                                           cAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTa                                            >  2:363487/1‑150 (MQ=255)
                                                                                                              ctgctgCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATc                                         <  2:2416980/150‑1 (MQ=255)
                                                                                                                 ctgcCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGtt                                      >  1:3883778/1‑150 (MQ=255)
                                                                                                                   gcCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGa                                    >  1:3598833/1‑150 (MQ=255)
                                                                                                                    ccGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGAc                                   <  1:2120346/150‑1 (MQ=255)
                                                                                                                     cGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACa                                  >  1:1339409/1‑150 (MQ=255)
                                                                                                                        tGGCTGCAACTGATGTACACAGATCACCTCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGCAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGat                               <  2:4128883/150‑1 (MQ=255)
                                                                                                                        tGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGat                               >  2:72617/1‑150 (MQ=255)
                                                                                                                        tGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGat                               >  2:1781372/1‑150 (MQ=255)
                                                                                                                            tGCAGCTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCt                           >  1:2551232/1‑150 (MQ=255)
                                                                                                                            tGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCt                           >  1:4341157/1‑150 (MQ=255)
                                                                                                                             gCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCtt                          <  2:78137/150‑1 (MQ=255)
                                                                                                                               aaCTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTcc                        >  1:2279889/1‑150 (MQ=255)
                                                                                                                                aCTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTcca                       <  1:2160669/150‑1 (MQ=255)
                                                                                                                                aCTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTcca                       <  2:4585375/150‑1 (MQ=255)
                                                                                                                                aCTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTcca                       <  2:3939074/150‑1 (MQ=255)
                                                                                                                                 cTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccac                      <  2:3850746/150‑1 (MQ=255)
                                                                                                                                 cTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccac                      <  2:3850416/150‑1 (MQ=255)
                                                                                                                                 cTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccac                      >  2:1965689/1‑150 (MQ=255)
                                                                                                                                 cTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccac                      <  2:227464/150‑1 (MQ=255)
                                                                                                                                  tGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccacc                     >  1:2970585/1‑150 (MQ=255)
                                                                                                                                  tGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccacc                     <  1:3964579/150‑1 (MQ=255)
                                                                                                                                   gATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccacca                    <  2:2128478/150‑1 (MQ=255)
                                                                                                                                    aTGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccaccac                   >  2:4256591/1‑150 (MQ=255)
                                                                                                                                      gTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccaccacca                 >  2:1354797/1‑150 (MQ=255)
                                                                                                                                           cAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATg            >  2:4082048/1‑150 (MQ=255)
                                                                                                                                              aTCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTtc         <  2:1481930/150‑1 (MQ=255)
                                                                                                                                               tCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTtct        <  1:3981135/150‑1 (MQ=255)
                                                                                                                                                cacaTCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTtctc       >  2:2073095/1‑150 (MQ=255)
                                                                                                                                                cacaTCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTtctc       >  1:1179788/1‑150 (MQ=255)
                                                                                                                                                  catcatCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTcgc     >  2:2668113/1‑150 (MQ=255)
                                                                                                                                                  catcatCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTcgc     >  2:1246399/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCGCTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCT  >  CP009273/2372980‑2373278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A