Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,425,587 C→A 8.7% D279Y (GAC→TAC)  folC ← bifunctional folylpolyglutamate synthase/ dihydrofolate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,425,5870CA 8.7% 1079.0 / 47.5 286D279Y (GAC→TAC) folCbifunctional folylpolyglutamate synthase/ dihydrofolate synthase
Reads supporting (aligned to +/- strand):  ref base C (128/133);  new base A (14/11);  total (142/144)
Fisher's exact test for biased strand distribution p-value = 5.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 288 total aligned reads displayed.

CCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGTCATGATCGGTGACGGAAT  >  CP009273/2425440‑2425736
                                                                                                                                                   |                                                                                                                                                     
ccAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTcgc                                                                                                                                                     >  1:1244936/1‑150 (MQ=255)
      cgcgCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATgg                                                                                                                                               <  1:514754/150‑1 (MQ=255)
      cgcgCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATgg                                                                                                                                               <  1:2347936/150‑1 (MQ=255)
       gcgcCCGTTTTTCGGTAGCGCTGTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGc                                                                                                                                              <  2:4467180/150‑1 (MQ=255)
        cgcCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCa                                                                                                                                             >  1:2491138/1‑150 (MQ=255)
        cgcCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTAGCGAATGGCa                                                                                                                                             >  1:3564368/1‑150 (MQ=255)
        cgcCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTAGCGAATGGCa                                                                                                                                             >  1:3329287/1‑150 (MQ=255)
          cccGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCAtt                                                                                                                                           >  1:1594725/1‑150 (MQ=255)
           ccGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCAttt                                                                                                                                          <  1:1035534/150‑1 (MQ=255)
           ccGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCAttt                                                                                                                                          <  1:595922/150‑1 (MQ=255)
           ccGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCAttt                                                                                                                                          >  2:2825519/1‑150 (MQ=255)
           ccGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCAttt                                                                                                                                          >  2:412785/1‑150 (MQ=255)
               ttttCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCAc                                                                                                                                      <  1:1366345/150‑1 (MQ=255)
                         cgcTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAg                                                                                                                            <  1:1181428/150‑1 (MQ=255)
                           cTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGcc                                                                                                                          >  2:497445/1‑150 (MQ=255)
                              tCATACGCCCGGGGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGc                                                                                                                       >  2:1432907/1‑150 (MQ=255)
                                aTACGCCCGGTGAGTTATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTAGCGAATGGCATTTTCACTGACTTCCAGCCCGCTg                                                                                                                     <  2:4416090/150‑1 (MQ=255)
                                 tACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTAGCGAATGGCATTTTCACTGACTTCCAGCCCGCTgg                                                                                                                    >  2:289809/1‑150 (MQ=255)
                                   cGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCa                                                                                                                  >  1:651732/1‑150 (MQ=255)
                                    gCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCAc                                                                                                                 <  2:2084718/150‑1 (MQ=255)
                                    gCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCAc                                                                                                                 >  2:1580901/1‑150 (MQ=255)
                                        ggTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGACTGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAg                                                                                                             >  1:141991/1‑150 (MQ=255)
                                        ggTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAg                                                                                                             >  1:2641476/1‑150 (MQ=255)
                                        ggTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAg                                                                                                             <  1:4320374/150‑1 (MQ=255)
                                        ggTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAg                                                                                                             >  1:1020152/1‑150 (MQ=255)
                                         gTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGt                                                                                                            <  2:191140/150‑1 (MQ=255)
                                          tGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTg                                                                                                           <  1:844112/150‑1 (MQ=255)
                                            agttatTCCGTCGCATGTGGATGATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCAGGCAAAATTGCGCTGGCAATCCCGGCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCCCGCAGTgcc                                                                                                         <  2:3572637/147‑1 (MQ=255)
                                             gaTATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCAGGCAATCCCGTAGCGAATGGCAATTTCACTGACTTCCAGCCCGCTGGCACGCAGTgccg                                                                                                        >  1:3747976/1‑150 (MQ=255)
                                               tatTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTgccgcc                                                                                                      >  2:643334/1‑150 (MQ=255)
                                                atTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCa                                                                                                     >  2:2624375/1‑150 (MQ=255)
                                                  tCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAgc                                                                                                   <  2:4003628/150‑1 (MQ=255)
                                                     gccgcATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCt                                                                                                >  1:2803136/1‑150 (MQ=255)
                                                     gccgcATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCt                                                                                                >  1:2115342/1‑150 (MQ=255)
                                                     gccgcATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCt                                                                                                <  2:3504965/150‑1 (MQ=255)
                                                      ccgcATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTg                                                                                               <  1:2759068/150‑1 (MQ=255)
                                                        gcATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGtt                                                                                             >  1:765964/1‑150 (MQ=255)
                                                        gcATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGtt                                                                                             <  1:1724475/150‑1 (MQ=255)
                                                        gcATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGtt                                                                                             <  1:1892047/150‑1 (MQ=255)
                                                        gcATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTAGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGtt                                                                                             <  1:3198975/150‑1 (MQ=255)
                                                           tgtgGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCg                                                                                          >  2:4348398/1‑150 (MQ=255)
                                                             tgGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGc                                                                                        >  2:958426/1‑150 (MQ=255)
                                                                 ttATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTc                                                                                    >  1:3447960/1‑150 (MQ=255)
                                                                 ttATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTc                                                                                    <  2:469895/150‑1 (MQ=255)
                                                                  tATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCg                                                                                   >  1:4551159/1‑150 (MQ=255)
                                                                     gcgcGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGtt                                                                                <  1:3047959/150‑1 (MQ=255)
                                                                       gcgACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGc                                                                              <  1:1869482/150‑1 (MQ=255)
                                                                               aaaaaTAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAg                                                                      <  2:1755027/150‑1 (MQ=255)
                                                                                 aaaTAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCg                                                                    >  2:4233044/1‑150 (MQ=255)
                                                                                  aaTAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCgg                                                                   >  1:4173606/1‑150 (MQ=255)
                                                                                  aaTAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCgg                                                                   <  2:252872/150‑1 (MQ=255)
                                                                                    tAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCa                                                                 <  2:4271164/150‑1 (MQ=255)
                                                                                     aaCGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCaa                                                                >  1:143994/1‑150 (MQ=255)
                                                                                     aaCGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCaa                                                                <  1:699703/150‑1 (MQ=255)
                                                                                      aCGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAAc                                                               <  2:4546312/150‑1 (MQ=255)
                                                                                       cgcgGGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACg                                                              <  1:1288191/150‑1 (MQ=255)
                                                                                        gcgTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTAGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACgg                                                             <  2:3024645/150‑1 (MQ=255)
                                                                                                 cTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTc                                                    <  1:2981031/150‑1 (MQ=255)
                                                                                                  tCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTcc                                                   <  2:2117402/150‑1 (MQ=255)
                                                                                                   cGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCa                                                  <  1:3840831/150‑1 (MQ=255)
                                                                                                    gCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACACGCGGCAACGGCAGATTGTCCAg                                                 >  2:242420/1‑150 (MQ=255)
                                                                                                    gCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAg                                                 <  2:32870/150‑1 (MQ=255)
                                                                                                    gCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAg                                                 <  2:6769/150‑1 (MQ=255)
                                                                                                     cTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGc                                                >  1:2618918/1‑150 (MQ=255)
                                                                                                      tCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTAGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCg                                               >  2:2395396/1‑150 (MQ=255)
                                                                                                          aaTCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGcc                                           <  1:1929567/150‑1 (MQ=255)
                                                                                                          aaTCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGcc                                           <  1:2377148/150‑1 (MQ=255)
                                                                                                              tGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGa                                       >  2:1110316/1‑150 (MQ=255)
                                                                                                              tGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGa                                       <  2:320820/150‑1 (MQ=255)
                                                                                                              tGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGa                                       >  2:3433975/1‑150 (MQ=255)
                                                                                                               ggAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAg                                      >  1:146890/1‑150 (MQ=255)
                                                                                                               ggAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAg                                      >  1:146988/1‑150 (MQ=255)
                                                                                                                gAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGc                                     >  2:77582/1‑150 (MQ=255)
                                                                                                                 aaaCGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCa                                    >  1:4420600/1‑150 (MQ=255)
                                                                                                                    cGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCg                                 >  1:4193046/1‑150 (MQ=255)
                                                                                                                      tCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCt                               >  2:870193/1‑150 (MQ=255)
                                                                                                                      tCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCt                               >  2:1415443/1‑150 (MQ=255)
                                                                                                                       cccGGCAAAATTGCGCTGGCAATCCCGTCGCGACTGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTa                              >  2:3874734/1‑150 (MQ=255)
                                                                                                                       cccGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTa                              >  1:2154979/1‑150 (MQ=255)
                                                                                                                         cGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTaaa                            >  1:3429876/1‑150 (MQ=255)
                                                                                                                          ggCAAAATTGCGCTGGCAATCCCGTAGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAAc                           >  2:2180920/1‑150 (MQ=255)
                                                                                                                           gCAAAATTGCGCTGGCAATCCCGTAGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACg                          <  1:1635763/150‑1 (MQ=255)
                                                                                                                              aaaTTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGccc                       <  2:1004108/150‑1 (MQ=255)
                                                                                                                              aaaTTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGccc                       <  2:4480069/150‑1 (MQ=255)
                                                                                                                               aaTTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCGACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCa                      >  1:3501816/1‑150 (MQ=255)
                                                                                                                               aaTTGCGCTGGCAATCCCGTAGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCa                      <  2:243360/150‑1 (MQ=255)
                                                                                                                                aTTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAg                     <  2:1180322/150‑1 (MQ=255)
                                                                                                                                aTTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAg                     >  2:937372/1‑150 (MQ=255)
                                                                                                                                  tGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGTACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGTc                   <  1:300970/150‑1 (MQ=255)
                                                                                                                                   gcgcTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGTCa                  <  1:9419/150‑1 (MQ=255)
                                                                                                                                    cgcTGGCAATCCCGTAGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGTCAt                 <  1:2899492/150‑1 (MQ=255)
                                                                                                                                      cTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGTCATGa               <  1:2525777/150‑1 (MQ=255)
                                                                                                                                          cAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGTCATGATCgg           >  2:4555397/1‑150 (MQ=255)
                                                                                                                                            aTCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGTCATGATCGGTg         <  1:2200432/150‑1 (MQ=255)
                                                                                                                                            aTCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGTCATGATCGGTg         >  1:2306488/1‑150 (MQ=255)
                                                                                                                                             tCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGTCATGATCGGTGa        >  2:3308995/1‑150 (MQ=255)
                                                                                                                                              cccGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGTCATGATCGGTGAc       <  2:1991329/150‑1 (MQ=255)
                                                                                                                                                  tCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGTCATGATCGGTGACGGaa   <  2:371924/150‑1 (MQ=255)
                                                                                                                                                  tCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGTCATGATCGGTGACGGaa   <  1:2630238/150‑1 (MQ=255)
                                                                                                                                                   cgcgAATGGCATTTTCACTGACTGCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGTCATGATCGGTGACGGact  >  2:1540164/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CCAGCACGCGCCCGTTTTTCGGTAGCGCTTTCATACGCCCGGTGAGATATTCCGCCGCATGTGGATTATGCGCGACATCAAAAATAACGCGTGGCGACTCGCTCACAATCTGGAAACGTCCCGGCAAAATTGCGCTGGCAATCCCGTCGCGAATGGCATTTTCACTGACTTCCAGCCCGCTGGCACGCAGTGCCGCCAGCGCTGTTGCGGCATTCGGTTGCGGGACAAGCGGCAACGGCAGATTTTCCAGCGTGCCGTGAGCATCGCTAAACGCCCAGTCATGATCGGTGACGGAAT  >  CP009273/2425440‑2425736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A