Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,438,410 C→A 20.5% A210A (GCG→GCT)  yfcA ← UPF0721 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,438,4100CA20.5% 729.5 / 126.9 253A210A (GCG→GCT) yfcAUPF0721 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (109/92);  new base A (16/36);  total (125/128)
Fisher's exact test for biased strand distribution p-value = 3.00e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 258 total aligned reads displayed.

TGTCCGTGGCTGTCGTAAAGTAATTTGGCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGG  >  CP009273/2438261‑2438559
                                                                                                                                                     |                                                                                                                                                     
  tCCGTGGCTGTCGTAAAGTAATTTGGCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGc                                                                                                                                                     <  2:1985696/150‑1 (MQ=255)
     gTGGCTGTCGTAAAGTAATTTGGCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCa                                                                                                                                                  <  1:3082621/150‑1 (MQ=255)
     gTGGCTGTCGTAAAGTAATTTGGCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCa                                                                                                                                                  >  2:3615206/1‑150 (MQ=255)
      tGGCTGTCGTAAAGTAATTTGGCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCaa                                                                                                                                                 >  1:2737199/1‑150 (MQ=255)
       ggCTGTCGTAAAGTAATTTGGCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGTCCGTAGCCCaaa                                                                                                                                                >  2:3766674/1‑150 (MQ=255)
            tCGTAAAGTAATTTGGCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACt                                                                                                                                           <  2:2935102/150‑1 (MQ=255)
               tAAAGTAATTTGGCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTg                                                                                                                                        >  2:221034/1‑150 (MQ=255)
               tAAAGTAATTTGGCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTg                                                                                                                                        >  2:4029408/1‑150 (MQ=255)
                aaaGTAATTTGGCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTgc                                                                                                                                       >  1:1884534/1‑150 (MQ=255)
                 aaGTAATTTGGCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTgcc                                                                                                                                      >  1:354525/1‑150 (MQ=255)
                  aGTAATTTGGCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTgccg                                                                                                                                     >  1:1031111/1‑150 (MQ=255)
                  aGTAATTTGGCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTgccg                                                                                                                                     <  1:3464469/150‑1 (MQ=255)
                    tAATTTGGCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTgccgcc                                                                                                                                   >  1:1147787/1‑150 (MQ=255)
                      aTTTGGCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCga                                                                                                                                 <  1:2429514/150‑1 (MQ=255)
                       tttGGCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCgag                                                                                                                                >  2:664208/1‑150 (MQ=255)
                         tGGCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGaa                                                                                                                              <  1:3889405/150‑1 (MQ=255)
                          ggCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGAAt                                                                                                                             <  1:731524/150‑1 (MQ=255)
                          ggCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGAAt                                                                                                                             <  1:624137/150‑1 (MQ=255)
                           gCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATa                                                                                                                            <  1:1383513/150‑1 (MQ=255)
                              cTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAAc                                                                                                                         <  1:1928382/150‑1 (MQ=255)
                               tcatcaCCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAAca                                                                                                                        <  1:905779/150‑1 (MQ=255)
                               tcatcaCCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAAca                                                                                                                        >  1:2529310/1‑150 (MQ=255)
                               tcatcaCCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGAATAAAca                                                                                                                        <  1:564475/150‑1 (MQ=255)
                               tcatcaCCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGAATAAAca                                                                                                                        <  2:2945438/150‑1 (MQ=255)
                                catcaCCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAAcag                                                                                                                       <  2:2046525/150‑1 (MQ=255)
                                catcaCCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAAcag                                                                                                                       <  2:2118272/150‑1 (MQ=255)
                                catcaCCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAAcag                                                                                                                       <  2:330684/150‑1 (MQ=255)
                                    aCCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGt                                                                                                                   <  1:4241598/150‑1 (MQ=255)
                                     ccgccgAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTa                                                                                                                  <  1:146898/150‑1 (MQ=255)
                                       gccgAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTaaa                                                                                                                >  2:1367408/1‑150 (MQ=255)
                                       gccgAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTaaa                                                                                                                >  2:3118308/1‑150 (MQ=255)
                                         cgAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATAACTTTGCCGCCGAGAATAAACAGCAGTAAAcc                                                                                                              >  1:115246/1‑150 (MQ=255)
                                          gagaCGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAAccg                                                                                                             <  1:3805313/150‑1 (MQ=255)
                                              cGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGa                                                                                                         <  1:2364188/150‑1 (MQ=255)
                                              cGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGa                                                                                                         >  2:3691572/1‑150 (MQ=255)
                                                aTAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATg                                                                                                       >  1:141814/1‑150 (MQ=255)
                                                  aaCAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGtt                                                                                                     <  2:711963/150‑1 (MQ=255)
                                                   aCAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGttt                                                                                                    <  2:4103016/150‑1 (MQ=255)
                                                   aCAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGttt                                                                                                    <  2:1464588/150‑1 (MQ=255)
                                                    cAATCATCGGGCGGATCAGCTTTTTTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTg                                                                                                   >  2:390815/1‑150 (MQ=255)
                                                    cAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTg                                                                                                   >  1:3065720/1‑150 (MQ=255)
                                                     aaTCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGa                                                                                                  >  2:3938261/1‑150 (MQ=255)
                                                      atcatcGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGAc                                                                                                 <  2:2541954/150‑1 (MQ=255)
                                                      atcatcGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGAc                                                                                                 <  1:2667248/150‑1 (MQ=255)
                                                      atcatcGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGAc                                                                                                 >  2:1115983/1‑150 (MQ=255)
                                                      atcatcGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGAc                                                                                                 <  2:795269/150‑1 (MQ=255)
                                                       tcatcGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGAcg                                                                                                >  2:2515708/1‑150 (MQ=255)
                                                          tcGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAAACAAATTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGAcgttg                                                                                             >  1:2061246/1‑150 (MQ=255)
                                                          tcGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGAcgttg                                                                                             <  1:743792/150‑1 (MQ=255)
                                                               cGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGAcgttgcgttg                                                                                        >  2:3383308/1‑150 (MQ=255)
                                                               cGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGAcgttgcgttg                                                                                        <  2:3477519/150‑1 (MQ=255)
                                                                ggATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAGACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGa                                                                                       <  2:1824057/150‑1 (MQ=255)
                                                                ggATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGa                                                                                       <  2:2805507/150‑1 (MQ=255)
                                                                  aTCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGt                                                                                     >  2:271349/1‑150 (MQ=255)
                                                                   tCAGCTTTTGTCCTTTGCTCAACACCAGTCGTTAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTa                                                                                    >  1:3471863/1‑150 (MQ=255)
                                                                     aGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAAt                                                                                  >  1:450290/1‑150 (MQ=255)
                                                                            gTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGt                                                                           >  1:1039817/1‑150 (MQ=255)
                                                                              ccTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGa                                                                         <  1:2188277/150‑1 (MQ=255)
                                                                              ccTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGa                                                                         >  1:1510742/1‑150 (MQ=255)
                                                                                 ttGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGcc                                                                      <  1:3302617/150‑1 (MQ=255)
                                                                                 ttGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGcc                                                                      <  1:1766515/150‑1 (MQ=255)
                                                                                  tGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCg                                                                     <  1:4518694/150‑1 (MQ=255)
                                                                                   gCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGt                                                                    <  2:189043/150‑1 (MQ=255)
                                                                                    cTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTg                                                                   >  1:3766148/1‑150 (MQ=255)
                                                                                    cTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTg                                                                   <  2:4277345/150‑1 (MQ=255)
                                                                                     tCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTgg                                                                  >  2:4205756/1‑150 (MQ=255)
                                                                                       aaCACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCt                                                                >  1:2425863/1‑150 (MQ=255)
                                                                                       aaCACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCt                                                                <  1:4461721/150‑1 (MQ=255)
                                                                                        acacCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCtt                                                               <  1:167673/150‑1 (MQ=255)
                                                                                         cacCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCttt                                                              <  1:2931417/150‑1 (MQ=255)
                                                                                           ccAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTgg                                                            >  2:527199/1‑150 (MQ=255)
                                                                                           ccAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTgg                                                            >  2:1303061/1‑150 (MQ=255)
                                                                                            cAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTGGAGTAATTTGGCGTGAGCCGTGGCTTTGGc                                                           >  2:2181542/1‑150 (MQ=255)
                                                                                             aGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCTATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCg                                                          >  1:209327/1‑150 (MQ=255)
                                                                                                 gTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGt                                                      <  2:4421350/150‑1 (MQ=255)
                                                                                                 gTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGt                                                      <  2:2758650/150‑1 (MQ=255)
                                                                                                    aaCCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTaa                                                   <  2:1148133/150‑1 (MQ=255)
                                                                                                    aaCCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTaa                                                   >  1:2805294/1‑150 (MQ=255)
                                                                                                     aCCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGAATAAACCGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTaaa                                                  <  2:1916408/150‑1 (MQ=255)
                                                                                                      cccATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAAt                                                 >  2:3978147/1‑150 (MQ=255)
                                                                                                        caagcgcgcgcCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGACGTGGCTTTGGCGAGGTTAAATcc                                               >  2:1238372/4‑150 (MQ=255)
                                                                                                        cATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATcc                                               <  1:256777/150‑1 (MQ=255)
                                                                                                         aTGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCg                                              >  1:4375631/1‑150 (MQ=255)
                                                                                                            cgcgcgcCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGcac                                           <  1:3427269/150‑1 (MQ=255)
                                                                                                                cgcCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCg                                       <  2:4504753/150‑1 (MQ=255)
                                                                                                                  ccGAGGAACTGCCCCACCAGCATGACAAAGCCCGTAGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTa                                     <  2:3564021/150‑1 (MQ=255)
                                                                                                                   cGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTaa                                    >  2:4309450/1‑150 (MQ=255)
                                                                                                                    gAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAAc                                   >  1:608151/1‑150 (MQ=255)
                                                                                                                         acggCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAgg                              <  2:4531610/147‑1 (MQ=255)
                                                                                                                         aCTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAgg                              <  1:1304497/150‑1 (MQ=255)
                                                                                                                           tGCCCCACCAGCATGAAAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATTAACAGCAGTAAACCTCCGATGTTTGACGTTGCTTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGTTTAAATCCGCACAGCGTAACGAAGGcc                            <  1:3678469/150‑1 (MQ=255)
                                                                                                                             ccccACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAg                          >  1:3196012/1‑150 (MQ=255)
                                                                                                                                      cATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTaaaa                 >  1:32373/1‑150 (MQ=255)
                                                                                                                                       aTGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAAc                >  1:3813819/1‑150 (MQ=255)
                                                                                                                                           cAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGAcc            <  1:2248922/150‑1 (MQ=255)
                                                                                                                                            aaaGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGAccc           <  2:4637852/150‑1 (MQ=255)
                                                                                                                                                 ccGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATTTTTGACGTTGCGTTGAGTATTTTGGCGTGAGCCGTGGCTTTGGCTAGTTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAg      <  1:4101798/150‑1 (MQ=255)
                                                                                                                                                   gTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAACCCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGcc    <  1:684102/150‑1 (MQ=255)
                                                                                                                                                   gTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGcc    <  1:1367408/150‑1 (MQ=255)
                                                                                                                                                     cGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCgg  >  2:349393/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGTCCGTGGCTGTCGTAAAGTAATTTGGCACTCATCACCGCCGAGACGATAACAATCATCGGGCGGATCAGCTTTTGTCCTTTGCTCAACACCAGTCGTGAACCCATGCGCGCGCCGAGGAACTGCCCCACCAGCATGACAAAGCCCGTCGCCCAAATCACTTTGCCGCCGAGAATAAACAGCAGTAAACCGCCGATGTTTGACGTTGCGTTGAGTAATTTGGCGTGAGCCGTGGCTTTGGCGAGGTTAAATCCGCACAGCGTAACGAAGGCCAGCGCGTAAAACGACCCGGCAGCCGG  >  CP009273/2438261‑2438559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A