Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,636,214 T→A 43.4% intergenic (‑11/+274) rodZ ← / ← rlmN MreB assembly cytoskeletal protein/dual specificity 23S rRNA m(2)A2503, tRNA m(2)A37 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,636,2140TA43.4% 115.7 / inf 235intergenic (‑11/+274)rodZ/rlmNMreB assembly cytoskeletal protein/dual specificity 23S rRNA m(2)A2503, tRNA m(2)A37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (56/77);  new base A (58/44);  total (114/121)
Fisher's exact test for biased strand distribution p-value = 2.61e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01

Only 100 of 238 total aligned reads displayed.

TGTCGCGTACCGTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTG  >  CP009273/2636065‑2636360
                                                                                                                                                     |                                                                                                                                                  
tGTCGCGTACCGTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGt                                                                                                                                                    <  1:2811979/150‑1 (MQ=255)
tGTCGCGTACCGTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGt                                                                                                                                                    <  1:4021454/150‑1 (MQ=255)
   cgcgTACCGTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACg                                                                                                                                                 >  2:4078341/1‑150 (MQ=255)
        aCCGTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTaa                                                                                                                                            >  2:4171094/1‑150 (MQ=255)
        aCCGTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTaa                                                                                                                                            <  2:476662/150‑1 (MQ=255)
          cGTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAAt                                                                                                                                          >  2:1641008/1‑150 (MQ=255)
           gTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATg                                                                                                                                         >  2:4605483/1‑150 (MQ=255)
           gTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATg                                                                                                                                         <  1:3403416/150‑1 (MQ=255)
           gTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATg                                                                                                                                         <  1:3402992/150‑1 (MQ=255)
            tGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATga                                                                                                                                        <  2:2511656/150‑1 (MQ=255)
             ggAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATgag                                                                                                                                       >  2:2448179/1‑150 (MQ=255)
             ggAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATgag                                                                                                                                       >  1:4581673/1‑150 (MQ=255)
             ggAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATgag                                                                                                                                       >  1:2545639/1‑150 (MQ=255)
               aaaCCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGaa                                                                                                                                     >  1:529580/1‑150 (MQ=255)
                  ccTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTg                                                                                                                                  >  2:1999711/1‑150 (MQ=255)
                   cTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTga                                                                                                                                 <  2:2900267/150‑1 (MQ=255)
                    ttCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTgag                                                                                                                                <  2:1388175/150‑1 (MQ=255)
                     tCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTgaga                                                                                                                               >  1:3714274/1‑150 (MQ=255)
                      cAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGAt                                                                                                                              <  1:3546407/150‑1 (MQ=255)
                       aGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATg                                                                                                                             >  2:1509004/1‑150 (MQ=255)
                       aGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTGCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATg                                                                                                                             >  2:1080323/1‑150 (MQ=255)
                           aaaGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCgg                                                                                                                         >  2:2203984/1‑150 (MQ=255)
                            aaGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGt                                                                                                                        >  1:224888/1‑150 (MQ=255)
                                  cTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACca                                                                                                                  >  2:639541/1‑150 (MQ=255)
                                   tCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACcat                                                                                                                 <  2:1176622/150‑1 (MQ=255)
                                        aaCGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGc                                                                                                            >  1:2449353/1‑150 (MQ=255)
                                        aaCGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGc                                                                                                            >  1:1044306/1‑150 (MQ=255)
                                         aCGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCt                                                                                                           >  1:167435/1‑150 (MQ=255)
                                           ggCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCa                                                                                                         >  1:351430/1‑150 (MQ=255)
                                                gctgACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCcac                                                                                                    >  2:3855771/1‑150 (MQ=255)
                                                gctgACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCcac                                                                                                    <  1:3554784/150‑1 (MQ=255)
                                                   gACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACAcc                                                                                                 <  1:1136181/150‑1 (MQ=255)
                                                   gACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACAcc                                                                                                 >  2:936542/1‑150 (MQ=255)
                                                       aaGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGa                                                                                             >  1:1932409/1‑150 (MQ=255)
                                                       aaGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGa                                                                                             <  1:381154/150‑1 (MQ=255)
                                                            cTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACAt                                                                                        <  2:1860573/150‑1 (MQ=255)
                                                                 tgttCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCgtt                                                                                   <  2:2742255/150‑1 (MQ=255)
                                                                 tgttCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCgtt                                                                                   <  2:3030895/150‑1 (MQ=255)
                                                                    tCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCgttaag                                                                                <  1:326531/150‑1 (MQ=255)
                                                                    tCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGACTTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCgttaag                                                                                >  2:3794680/1‑150 (MQ=255)
                                                                      gcgAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCgttaagtt                                                                              <  1:774622/150‑1 (MQ=255)
                                                                          gCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAAcc                                                                          <  2:152468/150‑1 (MQ=255)
                                                                          gCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAAcc                                                                          <  2:4124318/150‑1 (MQ=255)
                                                                            aTTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGt                                                                        >  2:859927/1‑150 (MQ=255)
                                                                             ttACGCAGGCGAGCGCCGGTAGTAAGTGCTTCANTTTGGTCGTNCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGtt                                                                       <  1:2931442/150‑1 (MQ=255)
                                                                                cGCAGGCGAGCGGCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATc                                                                    <  1:3194124/150‑1 (MQ=255)
                                                                                     gCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACa                                                               <  1:4183890/150‑1 (MQ=255)
                                                                                      cGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAg                                                              <  1:1730800/150‑1 (MQ=255)
                                                                                      cGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAg                                                              >  1:1213034/1‑150 (MQ=255)
                                                                                      cGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAg                                                              >  2:3303499/1‑150 (MQ=255)
                                                                                      cGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAg                                                              <  2:1553247/150‑1 (MQ=255)
                                                                                       gAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGt                                                             <  1:3435210/150‑1 (MQ=255)
                                                                                       gAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGt                                                             >  1:2315649/1‑150 (MQ=255)
                                                                                       gAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGt                                                             >  2:593443/1‑150 (MQ=255)
                                                                                       gAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGt                                                             >  2:593357/1‑150 (MQ=255)
                                                                                       gAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGt                                                             >  2:1532881/1‑150 (MQ=255)
                                                                                           gcCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATaa                                                         >  1:3368720/1‑150 (MQ=255)
                                                                                            ccGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAg                                                        <  1:150121/150‑1 (MQ=255)
                                                                                               gtagtaAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGAcc                                                     <  2:2578650/150‑1 (MQ=255)
                                                                                                tagtaAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCg                                                    <  2:3286002/150‑1 (MQ=255)
                                                                                                tagtaAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCg                                                    <  1:3834275/150‑1 (MQ=255)
                                                                                                tagtaAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCg                                                    <  2:1923219/150‑1 (MQ=255)
                                                                                                 agtaAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGt                                                   <  1:4277617/150‑1 (MQ=255)
                                                                                                 agtaAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGt                                                   >  1:1936619/1‑150 (MQ=255)
                                                                                                   taAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCt                                                 >  2:94831/1‑150 (MQ=255)
                                                                                                    aaGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCtg                                                <  1:4082019/150‑1 (MQ=255)
                                                                                                    aaGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCtg                                                >  1:844745/1‑150 (MQ=255)
                                                                                                       tGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCtgtgt                                             <  2:3613599/150‑1 (MQ=255)
                                                                                                        gCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTCTAAGACCGTCtgtgtg                                            >  1:4307897/1‑150 (MQ=255)
                                                                                                        gCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCtgtgtg                                            <  2:3718468/150‑1 (MQ=255)
                                                                                                        gCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCtgtgtg                                            <  1:4157699/150‑1 (MQ=255)
                                                                                                        gCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGAGGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCtgtgtg                                            <  2:1092596/150‑1 (MQ=255)
                                                                                                          ttCATTTTGGTCGTGCGTGGCTTCAGTATTCGTTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCt                                          >  2:3823431/1‑150 (MQ=255)
                                                                                                            cATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCg                                        <  1:1315291/150‑1 (MQ=255)
                                                                                                            cATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCg                                        <  1:50489/150‑1 (MQ=255)
                                                                                                            cATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCg                                        >  1:1860142/1‑150 (MQ=255)
                                                                                                             aTTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGt                                       >  2:3574629/1‑150 (MQ=255)
                                                                                                                   gTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACaaa                                 <  2:3261211/150‑1 (MQ=255)
                                                                                                                      gtgcgtgGCTTCAGTATTCAGTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGcc                              <  1:4044442/150‑1 (MQ=255)
                                                                                                                          gtgGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAATCATCCGTTAAGTTAACCGTTATCATCCAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCcaca                          <  1:905057/150‑1 (MQ=255)
                                                                                                                          gtgGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCcaca                          >  2:2739040/1‑150 (MQ=255)
                                                                                                                           tgGCTTCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCcacct                         >  2:4297548/1‑148 (MQ=255)
                                                                                                                             gCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTc                       <  2:3212085/150‑1 (MQ=255)
                                                                                                                              cTTCAGTATTCATTCGCTGCAGGTACGATTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCa                      <  1:3410990/150‑1 (MQ=255)
                                                                                                                                tCAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACt                    <  1:1795522/150‑1 (MQ=255)
                                                                                                                                 cAGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTa                   >  2:4100910/1‑150 (MQ=255)
                                                                                                                                  aGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTaa                  >  2:3343415/1‑150 (MQ=255)
                                                                                                                                  aGTATTCATTCGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTaa                  >  2:1385991/1‑150 (MQ=255)
                                                                                                                                        cattcGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTaa            <  2:4509465/150‑1 (MQ=255)
                                                                                                                                        cattcGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTaa            >  1:2506620/1‑150 (MQ=255)
                                                                                                                                         attcGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAAc           <  1:3028801/150‑1 (MQ=255)
                                                                                                                                          ttcGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACt          >  1:1570223/1‑150 (MQ=255)
                                                                                                                                          ttcGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACt          <  1:66427/150‑1 (MQ=255)
                                                                                                                                          ttcGCTGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACt          <  1:1021454/150‑1 (MQ=255)
                                                                                                                                              cTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACt      <  1:2867193/150‑1 (MQ=255)
                                                                                                                                               tGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTg     >  2:3163584/1‑150 (MQ=255)
                                                                                                                                               tGCAGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTg     <  1:1550253/150‑1 (MQ=255)
                                                                                                                                                 cAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGtt   <  1:2978869/150‑1 (MQ=255)
                                                                                                                                                 cAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGtt   <  2:718462/150‑1 (MQ=255)
                                                                                                                                                  aGGAACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTg  <  2:2125012/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TGTCGCGTACCGTGGAAACCTTCAGGCAAAGTCGCTCGGCAACGGCCTGCTGACTAAGTCCTAGTTGTTCGCGAGCATTACGCAGGCGAGCGCCGGTAGTAAGTGCTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTG  >  CP009273/2636065‑2636360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A