Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,709,841 T→A 13.5% intergenic (‑33/‑272) grcA ← / → ung autonomous glycyl radical cofactor/uracil‑DNA‑glycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,709,8410TA13.5% 874.1 / 104.4 289intergenic (‑33/‑272)grcA/ungautonomous glycyl radical cofactor/uracil‑DNA‑glycosylase
Reads supporting (aligned to +/- strand):  ref base T (125/125);  new base A (16/23);  total (141/148)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 296 total aligned reads displayed.

TGCATAACCTGCTTTTGCAACGATGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCG  >  CP009273/2709692‑2709990
                                                                                                                                                     |                                                                                                                                                     
tGCATAACCTGCTTTTGCAACGATGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGt                                                                                                                                                       <  1:2757198/150‑1 (MQ=255)
  cATAACCTGCTTTTGCAACGATGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTc                                                                                                                                                     <  1:3674846/150‑1 (MQ=255)
  cATAACCTGCTTTTGCAACGATGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGATc                                                                                                                                                     <  2:3986351/150‑1 (MQ=255)
     aaCCTGCTTTTGCAACGATGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGc                                                                                                                                                  >  2:8742/1‑150 (MQ=255)
        cTGCTTTTGCAACGATGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGTCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTg                                                                                                                                               >  2:2289928/1‑150 (MQ=255)
         tGCTTTTGCAACGATGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGt                                                                                                                                              <  2:2223075/150‑1 (MQ=255)
          gCTTTTGCAACGATGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTc                                                                                                                                             <  2:4112001/150‑1 (MQ=255)
            ttttGCAACGATGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGATCAGCCTGTcgc                                                                                                                                           >  1:1336879/1‑150 (MQ=255)
                 cAACGATGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCa                                                                                                                                      >  1:2435346/1‑150 (MQ=255)
                 cAACGAAGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCa                                                                                                                                      <  2:3842534/150‑1 (MQ=255)
                   gcGATGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAAt                                                                                                                                    >  1:2028297/2‑150 (MQ=255)
                     gATGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTg                                                                                                                                  <  1:1464101/150‑1 (MQ=255)
                     gATGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTg                                                                                                                                  >  1:69864/1‑150 (MQ=255)
                     gATGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTg                                                                                                                                  <  1:3826556/150‑1 (MQ=255)
                      aTGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTgg                                                                                                                                 >  1:3186585/1‑150 (MQ=255)
                      aTGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGATCAGCCTGTCGCGGCCAATTgg                                                                                                                                 <  1:1814640/150‑1 (MQ=255)
                      aTGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCAGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTgg                                                                                                                                 >  2:320850/1‑150 (MQ=255)
                       tGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGt                                                                                                                                <  1:436862/150‑1 (MQ=255)
                       tGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGt                                                                                                                                <  1:2314915/150‑1 (MQ=255)
                        gCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTa                                                                                                                               >  2:3061225/1‑150 (MQ=255)
                        gCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTa                                                                                                                               >  2:1745962/1‑150 (MQ=255)
                         cAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTaa                                                                                                                              >  2:1748142/1‑150 (MQ=255)
                              cgcTTCGCCTTTTTAGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCTTTATCGGCTTTAGTATTCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCa                                                                                                                         <  2:1433301/150‑1 (MQ=255)
                                cTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCAtt                                                                                                                       <  2:152412/150‑1 (MQ=255)
                                 ttCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTATCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCATCCCCGGCAAAAGTATTTGATGTGATCAGCCTGTCGAGGCCAATTTGTAAAACCAttg                                                                                                                      >  2:4261338/1‑150 (MQ=255)
                                 ttCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGATCAGCCTGTCGCGGCCAATTGGTAAAACCAttg                                                                                                                      >  2:1139668/1‑150 (MQ=255)
                                  tCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCAttgt                                                                                                                     >  2:4232426/1‑150 (MQ=255)
                                  tCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATATGGATACCTGTAATCATGTGATGCATCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCAttgg                                                                                                                     >  1:2206835/1‑149 (MQ=255)
                                   cGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCAttgtt                                                                                                                    >  1:1969380/1‑150 (MQ=255)
                                   cGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCAttgtt                                                                                                                    <  1:3018036/150‑1 (MQ=255)
                                     ccTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGc                                                                                                                  >  1:2518408/1‑150 (MQ=255)
                                     ccTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGc                                                                                                                  <  1:1735028/150‑1 (MQ=255)
                                            cGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAgtg                                                                                                           <  2:1505020/150‑1 (MQ=255)
                                            cGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGATCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAgtg                                                                                                           >  2:441698/1‑150 (MQ=255)
                                             gCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAgtgt                                                                                                          <  1:3928958/150‑1 (MQ=255)
                                              cTGTCCAGCAGCCAGACAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGta                                                                                                         <  1:3690613/150‑1 (MQ=255)
                                                gTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGtata                                                                                                       <  1:1093893/150‑1 (MQ=255)
                                                    agcagcCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGtatatata                                                                                                   >  1:1147420/1‑150 (MQ=255)
                                                    agcagcCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGtatatata                                                                                                   <  1:854518/150‑1 (MQ=255)
                                                      cagcCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGATCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATAct                                                                                                 >  2:1960359/1‑150 (MQ=255)
                                                       agcCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATActc                                                                                                >  1:4016854/1‑150 (MQ=255)
                                                         ccAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATActcct                                                                                              >  2:1172914/1‑150 (MQ=255)
                                                         ccAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATActcct                                                                                              <  2:800020/150‑1 (MQ=255)
                                                         ccAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGATCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATActcct                                                                                              <  2:3541795/150‑1 (MQ=255)
                                                          cAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATActcctc                                                                                             >  2:3707170/1‑150 (MQ=255)
                                                          cAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGATCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATActcctc                                                                                             >  2:4495205/1‑150 (MQ=255)
                                                           aGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCa                                                                                            >  1:4219595/1‑150 (MQ=255)
                                                            gAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCTGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCaa                                                                                           >  1:1604839/1‑150 (MQ=255)
                                                                gagTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGTTTTGATCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAacac                                                                                       <  2:4460131/150‑1 (MQ=255)
                                                                 agTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACAcc                                                                                      <  1:1242949/150‑1 (MQ=255)
                                                                   ttCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCt                                                                                    <  2:2142953/150‑1 (MQ=255)
                                                                    tCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGATCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCtt                                                                                   <  1:949053/150‑1 (MQ=255)
                                                                     cagcagATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTg                                                                                  >  1:1540380/1‑150 (MQ=255)
                                                                       gcagATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGATCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGaa                                                                                <  1:6795/150‑1 (MQ=255)
                                                                          gATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCt                                                                             >  2:3282460/1‑150 (MQ=255)
                                                                           aTCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCtt                                                                            <  2:1357879/150‑1 (MQ=255)
                                                                           aTCGTCGTTAGCGGCTTTAGGAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCTAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCtt                                                                            <  2:485667/150‑1 (MQ=255)
                                                                             cgtcgtTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTg                                                                          >  2:3257446/1‑150 (MQ=255)
                                                                             cgtcgtTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTg                                                                          >  1:58383/1‑150 (MQ=255)
                                                                                cgtTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGAtt                                                                       >  1:1015885/1‑150 (MQ=255)
                                                                                cgtTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGAtt                                                                       <  1:4468278/150‑1 (MQ=255)
                                                                                     gCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAAt                                                                  <  1:4116278/150‑1 (MQ=255)
                                                                                     gCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAAt                                                                  <  2:2376676/150‑1 (MQ=255)
                                                                                       ggCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCa                                                                >  1:3511628/1‑150 (MQ=255)
                                                                                           ttAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATaa                                                            <  1:4142781/150‑1 (MQ=255)
                                                                                                  tCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACcac                                                     <  2:2828275/150‑1 (MQ=255)
                                                                                                  tCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACcac                                                     <  1:4627198/150‑1 (MQ=255)
                                                                                                      gATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACAt                                                 <  2:2843813/150‑1 (MQ=255)
                                                                                                      gATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGATCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACAt                                                 <  2:3359745/150‑1 (MQ=255)
                                                                                                       aTACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGATCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATc                                                >  2:2061535/1‑150 (MQ=255)
                                                                                                          ccTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAg                                             <  2:1278568/150‑1 (MQ=255)
                                                                                                          ccTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAg                                             >  2:1632072/1‑150 (MQ=255)
                                                                                                           cTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGt                                            >  2:2722788/1‑150 (MQ=255)
                                                                                                            tGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTa                                           >  2:4465747/1‑150 (MQ=255)
                                                                                                             gTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTAt                                          >  2:4290925/1‑150 (MQ=255)
                                                                                                              tAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATg                                         <  2:4372955/150‑1 (MQ=255)
                                                                                                               aaTCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATgg                                        >  2:848744/1‑150 (MQ=255)
                                                                                                                  cATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCg                                     >  1:3143212/1‑150 (MQ=255)
                                                                                                                              ccccGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATCCTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGAtttt                         <  2:1605243/150‑1 (MQ=255)
                                                                                                                              ccccGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGAtttt                         <  1:4329903/150‑1 (MQ=255)
                                                                                                                              ccccGGCAAAATTATTTGATTTGATCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGAtttt                         <  2:3633611/150‑1 (MQ=255)
                                                                                                                                 cGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTAtt                      >  2:3335008/1‑150 (MQ=255)
                                                                                                                                 cGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTAtt                      <  2:4343712/150‑1 (MQ=255)
                                                                                                                                   gCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTATTGt                    <  1:3938444/150‑1 (MQ=255)
                                                                                                                                    cAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTATTGtt                   <  2:2333035/150‑1 (MQ=255)
                                                                                                                                    cAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTATTGtt                   >  2:2520718/1‑150 (MQ=255)
                                                                                                                                    cAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTATTGtt                   >  1:3322541/1‑150 (MQ=255)
                                                                                                                                         ttattTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTATTGTTTTACa              >  2:3387998/1‑150 (MQ=255)
                                                                                                                                              tgatttgTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTATTGTTTTACATCAAc         <  2:927082/150‑1 (MQ=255)
                                                                                                                                              tgatttgTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTATTGTTTTACATCAAc         <  2:3470193/150‑1 (MQ=255)
                                                                                                                                               gatttgTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTATTGTTTTACATCAACt        >  1:2596341/1‑150 (MQ=255)
                                                                                                                                               gatttgTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTATTGTTTTACATCAACt        <  2:2024945/150‑1 (MQ=255)
                                                                                                                                                atttgTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAACACCACACATCAAGTATGGTCGCAAATGGATTTTATTGTTTTACATCAACtt       <  1:535290/150‑1 (MQ=255)
                                                                                                                                                atgtgTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTATTGTTTTACATCAACtt       <  1:2648955/147‑1 (MQ=255)
                                                                                                                                                  ttgTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTAt     <  2:505153/150‑1 (MQ=255)
                                                                                                                                                  ttgTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTAt     <  2:254281/150‑1 (MQ=255)
                                                                                                                                                   tgTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATg    <  1:2893934/150‑1 (MQ=255)
                                                                                                                                                    gTTCATACTGTCGCGGCCATTTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCATCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAAACACACATCAAGTATGGTCGCAAATGGATTTTTTTGTTTTACATCAACTTATGc   <  1:320850/150‑1 (MQ=255)
                                                                                                                                                     ttCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCg  >  2:609034/1‑150 (MQ=255)
                                                                                                                                                     ttCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAACGGATTTTATTGTTTTACATCAACTTATGCg  <  1:3374142/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCATAACCTGCTTTTGCAACGATGCAACGCGCTTCGCCTTTTTCGCTGTCCAGCAGCCAGAAAGAGTTCAGCAGATCGTCGTTAGCGGCTTTAGTAATCTGGATACCTGTAATCATGTGATGCCTCCCCGGCAAAATTATTTGATTTGTTCAGCCTGTCGCGGCCAATTGGTAAAACCATTGTTGCTTGAGTGTATATATACTCCTCAAACACCCTTGAATCTTTGATTTAAATCAATAAAAACCACACATCAAGTATGGTCGCAAATGGATTTTATTGTTTTACATCAACTTATGCG  >  CP009273/2709692‑2709990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A