Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,761,733 T→A 6.8% V222E (GTA→GAA)  yfjP → CP4‑57 prophage; 50S ribosome‑binding GTPase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,761,7330TA 6.8% 961.9 / 46.3 295V222E (GTA→GAA) yfjPCP4‑57 prophage; 50S ribosome‑binding GTPase family protein
Reads supporting (aligned to +/- strand):  ref base T (147/128);  new base A (14/6);  total (161/134)
Fisher's exact test for biased strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

Only 100 of 307 total aligned reads displayed.

ACAAAATATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGC  >  CP009273/2761584‑2761882
                                                                                                                                                     |                                                                                                                                                     
    aaTATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGgtagta                                                                                                                                                   <  1:3600244/150‑1 (MQ=255)
    aaTATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGgtagta                                                                                                                                                   >  1:1805810/1‑150 (MQ=255)
       atCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAgcg                                                                                                                                                >  2:1436189/1‑150 (MQ=255)
       atCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAgcg                                                                                                                                                <  2:3314313/150‑1 (MQ=255)
       atCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAgcg                                                                                                                                                >  2:278843/1‑150 (MQ=255)
         cAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTACCGCt                                                                                                                                              >  2:4514161/1‑150 (MQ=255)
          aGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTa                                                                                                                                             >  1:2503211/1‑150 (MQ=255)
          aGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTa                                                                                                                                             <  2:2504732/150‑1 (MQ=255)
               cAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTTATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCa                                                                                                                                        >  1:1138502/1‑150 (MQ=255)
               cAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCa                                                                                                                                        >  1:3462694/1‑150 (MQ=255)
                aaaaTCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCaa                                                                                                                                       <  2:2266064/150‑1 (MQ=255)
                   aTCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACAt                                                                                                                                    >  2:3822248/1‑150 (MQ=255)
                    tCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATc                                                                                                                                   >  2:3073619/1‑150 (MQ=255)
                       gcctgcTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCt                                                                                                                                >  1:3493276/1‑150 (MQ=255)
                        cctgcTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCtt                                                                                                                               >  2:3228796/1‑150 (MQ=255)
                        cctgcTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCtt                                                                                                                               <  1:2572443/150‑1 (MQ=255)
                        cctgcTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCtt                                                                                                                               >  2:947215/1‑150 (MQ=255)
                          tgctgcATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTc                                                                                                                             >  1:9962/1‑150 (MQ=255)
                          tgctgcATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGAAGTAGCGCTACTGCAACATCCCTTTc                                                                                                                             >  1:3910510/1‑150 (MQ=255)
                             tgcATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGaa                                                                                                                          >  2:4607688/1‑150 (MQ=255)
                               cATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGaaca                                                                                                                        >  2:4431088/1‑150 (MQ=255)
                                aTGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGaacaa                                                                                                                       >  2:1529315/1‑150 (MQ=255)
                                aTGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGaacaa                                                                                                                       >  2:2870387/1‑150 (MQ=255)
                                aTGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGaacaa                                                                                                                       >  2:4091325/1‑150 (MQ=255)
                                  gAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACg                                                                                                                     <  1:1243581/150‑1 (MQ=255)
                                    gCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGt                                                                                                                   >  1:1948968/1‑150 (MQ=255)
                                      tATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAg                                                                                                                 >  1:3166271/1‑150 (MQ=255)
                                      tATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAg                                                                                                                 >  1:4322013/1‑150 (MQ=255)
                                       aTTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGc                                                                                                                >  2:2578620/1‑150 (MQ=255)
                                         tCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGccc                                                                                                              >  2:1800822/1‑150 (MQ=255)
                                          ccAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCg                                                                                                             >  1:1538824/1‑150 (MQ=255)
                                           cAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCgg                                                                                                            >  2:1922610/1‑150 (MQ=255)
                                            aGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCggg                                                                                                           <  2:1467344/150‑1 (MQ=255)
                                            aGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCggg                                                                                                           >  1:3003666/1‑150 (MQ=255)
                                             gCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGa                                                                                                          >  1:102499/1‑150 (MQ=255)
                                              cccGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAg                                                                                                         >  1:1316786/1‑150 (MQ=255)
                                               ccGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGc                                                                                                        >  1:3786154/1‑150 (MQ=255)
                                                  tGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAgg                                                                                                     <  2:371627/150‑1 (MQ=255)
                                                     aTCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCAc                                                                                                  <  1:2947307/150‑1 (MQ=255)
                                                      tCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACg                                                                                                 <  2:3786560/150‑1 (MQ=255)
                                                         ggTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGa                                                                                              <  2:2307934/150‑1 (MQ=255)
                                                              gtgCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGAAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAtt                                                                                         <  2:1268643/150‑1 (MQ=255)
                                                              gtgCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGAAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGAtt                                                                                         <  2:1014077/150‑1 (MQ=255)
                                                                gCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTc                                                                                       >  1:3339871/1‑150 (MQ=255)
                                                                      tCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGaa                                                                                 >  1:1870831/1‑150 (MQ=255)
                                                                       cGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGaaa                                                                                <  1:367657/150‑1 (MQ=255)
                                                                         gTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAAcc                                                                              <  2:4320089/150‑1 (MQ=255)
                                                                           tCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGt                                                                            <  2:3500717/150‑1 (MQ=255)
                                                                            cGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTa                                                                           >  2:4267551/1‑150 (MQ=255)
                                                                             gCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAg                                                                          >  2:3542077/1‑150 (MQ=255)
                                                                              ccTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAgg                                                                         <  2:1826708/150‑1 (MQ=255)
                                                                              ccTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAgg                                                                         <  1:2539197/150‑1 (MQ=255)
                                                                                  cAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGcc                                                                     >  2:1927641/1‑150 (MQ=255)
                                                                                   aGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCa                                                                    >  1:1047773/1‑150 (MQ=255)
                                                                                    gTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCAt                                                                   >  1:3339155/1‑150 (MQ=255)
                                                                                       gggACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACt                                                                >  1:482611/1‑150 (MQ=255)
                                                                                       gggACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACt                                                                <  2:584500/150‑1 (MQ=255)
                                                                                       gggACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGACGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACt                                                                >  2:1973484/1‑150 (MQ=255)
                                                                                         gACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCg                                                              >  2:2111566/1‑150 (MQ=255)
                                                                                          aCTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGAAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGa                                                             >  2:314898/1‑150 (MQ=255)
                                                                                            tAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                                                           <  2:4151989/150‑1 (MQ=255)
                                                                                            tAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATa                                                           >  2:2414116/1‑150 (MQ=255)
                                                                                                ggTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGt                                                       >  1:3191156/1‑150 (MQ=255)
                                                                                                ggTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGt                                                       <  1:2791608/150‑1 (MQ=255)
                                                                                                ggTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGt                                                       <  2:392078/150‑1 (MQ=255)
                                                                                                ggTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGt                                                       <  1:4333168/150‑1 (MQ=255)
                                                                                                   gATGGCAGAGCTGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACGGCAACATCCCTTTCGAACATCGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAg                                                    <  2:695112/150‑1 (MQ=255)
                                                                                                    aTGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGc                                                   >  2:2705860/1‑150 (MQ=255)
                                                                                                        cAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt                                               <  1:1733587/150‑1 (MQ=255)
                                                                                                        cAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt                                               <  1:1634225/150‑1 (MQ=255)
                                                                                                        cAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt                                               <  2:480502/150‑1 (MQ=255)
                                                                                                        cAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt                                               <  2:1575190/150‑1 (MQ=255)
                                                                                                        cAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCt                                               >  1:1461731/1‑150 (MQ=255)
                                                                                                          gagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCttt                                             <  2:2650758/150‑1 (MQ=255)
                                                                                                          gagCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCttt                                             <  2:850225/150‑1 (MQ=255)
                                                                                                           agCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTc                                            <  1:552018/150‑1 (MQ=255)
                                                                                                             cGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTccc                                          >  2:1081205/1‑150 (MQ=255)
                                                                                                             cGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTccc                                          >  2:3972456/1‑150 (MQ=255)
                                                                                                              ggATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTcccc                                         >  1:3857530/1‑150 (MQ=255)
                                                                                                                  gatTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTAt                                     >  1:2858123/1‑150 (MQ=255)
                                                                                                                     tAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGTTACGGTAAGCACCTTTCCCCTTATTcc                                  <  1:3153383/150‑1 (MQ=255)
                                                                                                                     tAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTcc                                  <  1:2378235/150‑1 (MQ=255)
                                                                                                                     tAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGAAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACNCGATACGGTAAGCACCTTTCCCCTTATTcc                                  <  1:4281367/150‑1 (MQ=255)
                                                                                                                      aaGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTccc                                 >  2:3245809/1‑150 (MQ=255)
                                                                                                                      aaGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTccc                                 <  1:1402827/150‑1 (MQ=255)
                                                                                                                        gtgtCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGc                               <  2:1714562/150‑1 (MQ=255)
                                                                                                                         tgtCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGcc                              <  2:3195355/150‑1 (MQ=255)
                                                                                                                          gtCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGccc                             <  2:335913/150‑1 (MQ=255)
                                                                                                                           tCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGcccc                            <  1:4568418/150‑1 (MQ=255)
                                                                                                                               ccGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTg                        <  2:4540874/150‑1 (MQ=255)
                                                                                                                                  cgTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCgg                     >  1:4438203/1‑150 (MQ=255)
                                                                                                                                    tGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGAc                   <  1:2272395/150‑1 (MQ=255)
                                                                                                                                    tGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGAc                   <  1:95524/150‑1 (MQ=255)
                                                                                                                                     gAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGAcc                  <  2:3449498/150‑1 (MQ=255)
                                                                                                                                     gAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGAcc                  <  2:2525283/150‑1 (MQ=255)
                                                                                                                                       ggCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCat                >  1:3805445/1‑150 (MQ=255)
                                                                                                                                               gcCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAgg        >  1:2223421/1‑150 (MQ=255)
                                                                                                                                                    gtagtaGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTg   >  1:4302170/1‑150 (MQ=255)
                                                                                                                                                     tagtagCGCTACTGCAACATCCCTTTCTAACAACGGTAGCCCGGGAGCATGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACACGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGc  >  2:1196543/1‑150 (MQ=255)
                                                                                                                                                     tagtagCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGc  >  2:2034453/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACAAAATATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGC  >  CP009273/2761584‑2761882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A