Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,783,587 C→A 19.7% R83S (CGC→AGC)  lhgO → L‑2‑hydroxyglutarate oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,783,5870CA19.7% 807.9 / 131.5 274R83S (CGC→AGC) lhgOL‑2‑hydroxyglutarate oxidase
Reads supporting (aligned to +/- strand):  ref base C (115/105);  new base A (34/20);  total (149/125)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 276 total aligned reads displayed.

AAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACC  >  CP009273/2783438‑2783736
                                                                                                                                                     |                                                                                                                                                     
aaaaaGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTc                                                                                                                                                       <  2:1684977/150‑1 (MQ=255)
  aaaGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGc                                                                                                                                                     >  2:1474714/1‑150 (MQ=255)
   aaGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGACACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCt                                                                                                                                                    >  1:580068/1‑150 (MQ=255)
   aaGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCt                                                                                                                                                    <  1:3684171/150‑1 (MQ=255)
    agagTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTa                                                                                                                                                   >  2:2810250/1‑150 (MQ=255)
     gagTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTAc                                                                                                                                                  >  1:4143371/1‑150 (MQ=255)
      agTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACg                                                                                                                                                 <  2:729115/150‑1 (MQ=255)
            ccccGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACt                                                                                                                                           <  1:938397/150‑1 (MQ=255)
            ccccGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACt                                                                                                                                           <  1:3603649/150‑1 (MQ=255)
              ccGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGc                                                                                                                                         >  2:2369526/1‑150 (MQ=255)
               cGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCg                                                                                                                                        <  2:1014455/150‑1 (MQ=255)
                    tGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAg                                                                                                                                   >  2:3653452/1‑150 (MQ=255)
                    tGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCAAg                                                                                                                                   >  1:2918706/1‑150 (MQ=255)
                     gTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGa                                                                                                                                  >  1:3386162/1‑150 (MQ=255)
                           aGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTgg                                                                                                                            <  2:2559106/150‑1 (MQ=255)
                           aGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCAAGATGCTgg                                                                                                                            <  1:3802598/150‑1 (MQ=255)
                           aGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCAAGATGCTgg                                                                                                                            <  1:3123137/150‑1 (MQ=255)
                              cGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTg                                                                                                                         <  2:4545405/150‑1 (MQ=255)
                               gggCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCAAGATGCTGGTTGc                                                                                                                        >  2:4159924/1‑150 (MQ=255)
                                ggCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGcc                                                                                                                       <  1:4060503/150‑1 (MQ=255)
                                ggCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGcc                                                                                                                       <  1:4449732/150‑1 (MQ=255)
                                ggCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCAAGATGCTGGTTGcc                                                                                                                       <  2:2961308/150‑1 (MQ=255)
                                    acaacaGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGt                                                                                                                   <  2:1179884/150‑1 (MQ=255)
                                      aacaGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTcc                                                                                                                 >  1:347387/1‑150 (MQ=255)
                                            ggcgTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGAtctc                                                                                                           <  1:3176138/150‑1 (MQ=255)
                                            ggcgTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGAtctc                                                                                                           <  2:1754176/150‑1 (MQ=255)
                                              cgTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGa                                                                                                         >  2:3685980/1‑150 (MQ=255)
                                               gTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGaa                                                                                                        <  1:1341404/150‑1 (MQ=255)
                                                tGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGaaa                                                                                                       <  1:2534291/150‑1 (MQ=255)
                                                 gATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAAt                                                                                                      <  2:1955398/150‑1 (MQ=255)
                                                      aTGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAAc                                                                                                 <  1:46022/150‑1 (MQ=255)
                                                      aTGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAAc                                                                                                 >  1:654246/1‑150 (MQ=255)
                                                       tGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACg                                                                                                <  2:1927474/150‑1 (MQ=255)
                                                          cGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGAt                                                                                             >  1:710244/1‑150 (MQ=255)
                                                          cGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGAt                                                                                             <  2:474922/150‑1 (MQ=255)
                                                          cGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGAt                                                                                             <  2:66669/150‑1 (MQ=255)
                                                           ggggTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATg                                                                                            >  1:565549/1‑150 (MQ=255)
                                                              gTCTATTACACGCCCGGCAGCCTGAAGGAGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAACGCCTTTTGCGATCAAAACGGCATTCGCTTCGACAACTGCTGCAAGATGATGGTTTCCACGTCCGATCTCGAAATGGAACGGATtcgc                                                                                         <  2:2175191/150‑1 (MQ=255)
                                                               tCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATgcgcg                                                                                        >  1:2543876/1‑150 (MQ=255)
                                                               tCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATgcgcg                                                                                        >  2:2086099/1‑150 (MQ=255)
                                                                 tATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGcc                                                                                      >  2:4630430/1‑150 (MQ=255)
                                                                    tACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAACGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTa                                                                                   <  1:1406406/150‑1 (MQ=255)
                                                                     acacGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTAt                                                                                  >  1:2425500/1‑150 (MQ=255)
                                                                     acacGCCAGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTAt                                                                                  >  2:3467797/1‑150 (MQ=255)
                                                                      cacGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATg                                                                                 >  1:3548404/1‑150 (MQ=255)
                                                                       acGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATgg                                                                                <  1:3974996/150‑1 (MQ=255)
                                                                          cccGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGaa                                                                             >  2:908271/1‑150 (MQ=255)
                                                                           ccGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAAc                                                                            >  1:2374554/1‑150 (MQ=255)
                                                                           ccGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAAc                                                                            >  1:2892631/1‑150 (MQ=255)
                                                                             ggCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGc                                                                          >  2:3345404/1‑150 (MQ=255)
                                                                             ggCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGc                                                                          >  1:2717780/1‑150 (MQ=255)
                                                                               cAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGcac                                                                        >  2:3301524/1‑150 (MQ=255)
                                                                                aGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGAGCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGcaca                                                                       >  2:605108/1‑150 (MQ=255)
                                                                                   cTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAgcg                                                                    >  2:3123971/1‑150 (MQ=255)
                                                                                      aaGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAgcggcg                                                                 >  1:3808106/1‑150 (MQ=255)
                                                                                       aGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGa                                                                >  2:3749956/1‑150 (MQ=255)
                                                                                        ggCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGaa                                                               <  1:678675/150‑1 (MQ=255)
                                                                                        ggCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGaa                                                               >  2:1350408/1‑150 (MQ=255)
                                                                                         gcgcAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAAc                                                              <  2:553971/150‑1 (MQ=255)
                                                                                         gcgcAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAAc                                                              <  2:4334232/150‑1 (MQ=255)
                                                                                          cgcAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACg                                                             >  1:1499527/1‑150 (MQ=255)
                                                                                          cgcAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACg                                                             >  2:76830/1‑150 (MQ=255)
                                                                                            cAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGt                                                           >  2:3269836/1‑150 (MQ=255)
                                                                                             aGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGTGCGCCTTATGGGAACGCACAGCGGCGAACGGTa                                                          <  1:3541917/150‑1 (MQ=255)
                                                                                             aGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTa                                                          >  2:1500536/1‑150 (MQ=255)
                                                                                                  tGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAg                                                     >  2:78778/1‑150 (MQ=255)
                                                                                                   gCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAACACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAgc                                                    <  1:3292/150‑1 (MQ=255)
                                                                                                   gCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAgc                                                    >  2:3841705/1‑150 (MQ=255)
                                                                                                   gCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAgc                                                    <  2:4013855/150‑1 (MQ=255)
                                                                                                    ccTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAgcg                                                   <  2:408022/150‑1 (MQ=255)
                                                                                                      tGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGcgcg                                                 <  1:859606/150‑1 (MQ=255)
                                                                                                         cGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAAt                                              <  2:3521265/150‑1 (MQ=255)
                                                                                                             aaaCCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCt                                          >  2:3948495/1‑150 (MQ=255)
                                                                                                              aaCCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCtt                                         >  1:1193128/1‑150 (MQ=255)
                                                                                                               aCCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTa                                        >  2:4367943/1‑150 (MQ=255)
                                                                                                                 cgcgCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAAc                                      <  2:4209943/150‑1 (MQ=255)
                                                                                                                   cgcCACCAAAGCCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGc                                    >  1:1006344/1‑150 (MQ=255)
                                                                                                                   cgcCACCAAAGCCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGc                                    >  2:852365/1‑150 (MQ=255)
                                                                                                                    gcCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGcc                                   >  2:3072520/1‑150 (MQ=255)
                                                                                                                    gcCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGcc                                   <  2:2110985/150‑1 (MQ=255)
                                                                                                                      caccaAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCcga                                 >  1:3029388/1‑150 (MQ=255)
                                                                                                                       accaAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCcgac                                >  2:1755895/1‑150 (MQ=255)
                                                                                                                       accaAAGCCTTTTGCGATCAAAACGGCATTAGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCcgac                                >  2:1400103/1‑150 (MQ=255)
                                                                                                                          aaaGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGaa                             >  2:1285916/1‑150 (MQ=255)
                                                                                                                          aaaGCCTATTGCGATCACAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTCTGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCcgacgac                             >  2:3121257/1‑149 (MQ=255)
                                                                                                                            aGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACt                           >  1:1659167/1‑150 (MQ=255)
                                                                                                                            aGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACt                           >  1:4480808/1‑150 (MQ=255)
                                                                                                                            aGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACt                           >  1:2110640/1‑150 (MQ=255)
                                                                                                                              ccTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCNAGCGCGAATGGCTTAACGCCGACGAACTgc                         <  1:4287848/150‑1 (MQ=255)
                                                                                                                               cTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcg                        >  1:4407318/1‑150 (MQ=255)
                                                                                                                                 tttGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgcg                      <  1:3345404/150‑1 (MQ=255)
                                                                                                                                  ttGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgcga                     <  1:2478156/150‑1 (MQ=255)
                                                                                                                                  ttGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgcga                     <  1:4068968/150‑1 (MQ=255)
                                                                                                                                     cGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTgcgcgagcg                  >  2:1455253/1‑150 (MQ=255)
                                                                                                                                          aaaaCGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCCAACTCGAAATGGAACGGATGCGCGCCTTATGGAAACGCACAGCGCCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAAc             >  1:1376023/1‑150 (MQ=255)
                                                                                                                                            aaCGGCATTAGCTACGACAACTGCGTCAAGATGCTGGTAGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGAGGCGAACGGTATCGAGCGCGAATGGCTTAAAGCCGACGAACTGCGCGAGCGCGAACCg           >  1:4557329/1‑150 (MQ=255)
                                                                                                                                              cGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGaa         <  2:2506059/150‑1 (MQ=255)
                                                                                                                                                gCATTAGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAata       >  1:1921635/1‑150 (MQ=255)
                                                                                                                                                  aTTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATc     <  1:1902024/150‑1 (MQ=255)
                                                                                                                                                    tCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCAc   >  1:1793228/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAAAAGAGTCCGCCCCGGCCTGTCACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGCCTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAACGGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAACGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAACGCCGACGAACTGCGCGAGCGCGAACCGAATATCACC  >  CP009273/2783438‑2783736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A