Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,074,352 C→T 15.7% D215N (GAC→AAC)  tktA ← transketolase 1, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,074,3520CT15.7% 718.6 / 101.8 254D215N (GAC→AAC) tktAtransketolase 1, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base C (97/117);  new base T (21/19);  total (118/136)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 259 total aligned reads displayed.

CGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTT  >  CP009273/3074203‑3074501
                                                                                                                                                     |                                                                                                                                                     
 ggcgCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTcg                                                                                                                                                      >  2:3247572/1‑150 (MQ=255)
   cgcACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTcgcg                                                                                                                                                    >  2:288825/1‑150 (MQ=255)
    gcACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGa                                                                                                                                                   <  2:1825567/150‑1 (MQ=255)
    gcACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGa                                                                                                                                                   >  1:18397/1‑150 (MQ=255)
     cACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGaa                                                                                                                                                  <  2:2281740/150‑1 (MQ=255)
           gggAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGt                                                                                                                                            >  2:3545891/1‑150 (MQ=255)
             gAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTTGCGAATAACGTGc                                                                                                                                          >  1:1199928/1‑150 (MQ=255)
                tCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAg                                                                                                                                       >  1:890700/1‑150 (MQ=255)
                   tGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCg                                                                                                                                    >  2:118094/1‑150 (MQ=255)
                   tGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCg                                                                                                                                    <  1:2600289/150‑1 (MQ=255)
                      gTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTaa                                                                                                                                 >  2:4333526/1‑150 (MQ=255)
                         ccGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCt                                                                                                                              <  1:1518769/150‑1 (MQ=255)
                         ccGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCt                                                                                                                              <  1:3740232/150‑1 (MQ=255)
                          cGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCtt                                                                                                                             <  1:31202/150‑1 (MQ=255)
                          cGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCtt                                                                                                                             >  1:324086/1‑150 (MQ=255)
                            gCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCg                                                                                                                           <  2:138747/150‑1 (MQ=255)
                             cTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGa                                                                                                                          >  1:16804/1‑150 (MQ=255)
                              tttGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGaa                                                                                                                         <  2:3055757/150‑1 (MQ=255)
                                  ttCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGc                                                                                                                     <  1:4095903/150‑1 (MQ=255)
                                     ggggAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCAtt                                                                                                                  <  1:3694772/150‑1 (MQ=255)
                                     ggggAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCAtt                                                                                                                  <  1:4540153/150‑1 (MQ=255)
                                     ggggAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCAtt                                                                                                                  <  1:2378939/150‑1 (MQ=255)
                                     ggggAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCAtt                                                                                                                  <  1:2523527/150‑1 (MQ=255)
                                      gggAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTg                                                                                                                 <  1:1235817/150‑1 (MQ=255)
                                       ggAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTTGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGc                                                                                                                <  2:2513791/150‑1 (MQ=255)
                                        gAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCg                                                                                                               >  1:2972176/1‑150 (MQ=255)
                                         aaccgaaaccgaTGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTAATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCgg                                                                                                              >  1:2223360/1‑150 (MQ=255)
                                          accgaaaccgaTGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTTGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGgt                                                                                                             >  2:1538701/1‑150 (MQ=255)
                                          accgaaaccgaTGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTTGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGgt                                                                                                             >  2:4026552/1‑150 (MQ=255)
                                          accgaaaccgaTGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGgt                                                                                                             >  1:3433756/1‑150 (MQ=255)
                                            cgaaaccgaTGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGgtgt                                                                                                           >  1:2028178/1‑150 (MQ=255)
                                             gaaaccgaTGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTgtc                                                                                                          >  2:458640/1‑150 (MQ=255)
                                              aaactgaTGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTgtcg                                                                                                         <  2:2089634/150‑1 (MQ=255)
                                              aaaccgaTGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTgtcg                                                                                                         >  1:483080/1‑150 (MQ=255)
                                              aaaccgaTGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTgtcg                                                                                                         <  1:1800807/150‑1 (MQ=255)
                                               aaccgaTGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTgtcgt                                                                                                        >  2:3366646/1‑150 (MQ=255)
                                                accgaTGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTgtcgtc                                                                                                       >  1:4121825/1‑150 (MQ=255)
                                                  cgaTGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCgg                                                                                                     >  2:788934/1‑150 (MQ=255)
                                                  cgaTGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCgg                                                                                                     >  2:532478/1‑150 (MQ=255)
                                                  cgaTGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCgg                                                                                                     <  2:2988658/150‑1 (MQ=255)
                                                      gatgGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGaa                                                                                                 <  1:140090/150‑1 (MQ=255)
                                                      gatgGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGaa                                                                                                 <  1:2455712/150‑1 (MQ=255)
                                                         ggTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCa                                                                                              <  2:751917/150‑1 (MQ=255)
                                                               gCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTTGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTc                                                                                        <  2:1904154/150‑1 (MQ=255)
                                                               gCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTc                                                                                        <  2:1981154/150‑1 (MQ=255)
                                                               gCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTc                                                                                        <  2:2735245/150‑1 (MQ=255)
                                                                        cagGGAAGGTTTGTCAGTCACTGCTCGCGCTTCTTCTACTGCGCGTTAGATAGATGCCGCTTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGAcc                                                                               >  2:1475686/1‑150 (MQ=255)
                                                                        cagGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGAcc                                                                               >  2:100748/1‑150 (MQ=255)
                                                                            gAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCg                                                                           <  1:2541279/150‑1 (MQ=255)
                                                                             aaGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGa                                                                          >  2:4309326/1‑150 (MQ=255)
                                                                             aaGGTTTGTCAGTCACTGCGCGCGCGTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTTGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGa                                                                          <  2:162358/150‑1 (MQ=255)
                                                                               ggTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATa                                                                        <  1:1016339/150‑1 (MQ=255)
                                                                               ggTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATa                                                                        <  1:46906/150‑1 (MQ=255)
                                                                               ggTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATa                                                                        >  1:2386984/1‑150 (MQ=255)
                                                                                    gtcagtcaCTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTTGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAAt                                                                   >  2:1437925/1‑150 (MQ=255)
                                                                                     tcagtcaCTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATa                                                                  <  1:3948434/150‑1 (MQ=255)
                                                                                     tcagtcaCTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATa                                                                  <  1:3398408/150‑1 (MQ=255)
                                                                                          caCTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTTGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGtt                                                             <  2:4215832/150‑1 (MQ=255)
                                                                                            cTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGt                                                           >  1:3856249/1‑150 (MQ=255)
                                                                                             tGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTc                                                          >  1:2190363/1‑150 (MQ=255)
                                                                                              gcgcgcgcTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCa                                                         <  1:1090321/150‑1 (MQ=255)
                                                                                               cgcgcgcTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTTGCGAATAACGTGCCAGCCGTAAGATTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCAt                                                        >  1:86200/1‑150 (MQ=255)
                                                                                                gcgcgcTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATc                                                       <  2:1366367/150‑1 (MQ=255)
                                                                                                 cgcgcTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCg                                                      >  1:60658/1‑150 (MQ=255)
                                                                                                  gcgcTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTTGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGt                                                     <  1:4233287/150‑1 (MQ=255)
                                                                                                    gcTTCTCCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAg                                                   <  2:395327/150‑1 (MQ=255)
                                                                                                        cttctACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTTGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATg                                               <  2:1864084/150‑1 (MQ=255)
                                                                                                          tctACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCa                                             <  1:3685797/150‑1 (MQ=255)
                                                                                                            tACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTTGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAAt                                           >  2:2452405/1‑150 (MQ=255)
                                                                                                             aCTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATc                                          <  2:3714381/150‑1 (MQ=255)
                                                                                                              cTGCTCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCa                                         <  2:3850530/150‑1 (MQ=255)
                                                                                                              cTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTTGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCa                                         <  2:4261099/150‑1 (MQ=255)
                                                                                                                  gcgTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGttt                                     <  2:1747349/150‑1 (MQ=255)
                                                                                                                   cgTTTGATAGATGCCGCGTCATGACCGTCGATGTTGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTa                                    >  2:110329/1‑150 (MQ=255)
                                                                                                                    gTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTAc                                   <  2:1243459/150‑1 (MQ=255)
                                                                                                                     tttGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTAcc                                  <  1:2704870/150‑1 (MQ=255)
                                                                                                                       tGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCATTGTCATCGTAGAATGCAATCAGTTTACCCa                                <  1:587208/150‑1 (MQ=255)
                                                                                                                        gATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAg                               <  1:288825/150‑1 (MQ=255)
                                                                                                                             aTGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCa                          <  1:3355629/150‑1 (MQ=255)
                                                                                                                              tGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAg                         <  2:3184148/150‑1 (MQ=255)
                                                                                                                              tGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAg                         <  2:3621224/150‑1 (MQ=255)
                                                                                                                               gCCGCGTCATGACCGTCGATGTTGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGc                        <  2:3441453/150‑1 (MQ=255)
                                                                                                                                ccGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCg                       <  1:4005706/150‑1 (MQ=255)
                                                                                                                                ccGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCg                       >  1:3586281/1‑150 (MQ=255)
                                                                                                                                 cgcgTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGt                      <  1:374508/150‑1 (MQ=255)
                                                                                                                                        tGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGcc               >  2:3886193/1‑150 (MQ=255)
                                                                                                                                         gACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGTACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCa              <  2:1483843/150‑1 (MQ=255)
                                                                                                                                         gACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCa              >  2:4152880/1‑150 (MQ=255)
                                                                                                                                          aCCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCag             <  1:3039854/150‑1 (MQ=255)
                                                                                                                                           ccGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCaga            >  2:2925152/1‑150 (MQ=255)
                                                                                                                                             gTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCagaga          >  1:4367078/1‑150 (MQ=255)
                                                                                                                                               cGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGc        >  2:2961053/1‑150 (MQ=255)
                                                                                                                                               cGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGc        <  2:483080/150‑1 (MQ=255)
                                                                                                                                                gATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCa       >  2:3156661/1‑150 (MQ=255)
                                                                                                                                                gATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCa       >  2:3377644/1‑150 (MQ=255)
                                                                                                                                                   gTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAAc    <  1:646628/150‑1 (MQ=255)
                                                                                                                                                   gTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAAc    >  2:230741/1‑150 (MQ=255)
                                                                                                                                                    tCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACt   <  1:350621/150‑1 (MQ=255)
                                                                                                                                                    tCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACt   <  2:60688/150‑1 (MQ=255)
                                                                                                                                                    tCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCAGTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACt   <  2:1049273/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGCGCACCGTGGGAGTCGTGGGTACCGGCTTTGTTCGGGGAACCGAAACCGATGATGGTTTTGCACATCAGCAGGGAAGGTTTGTCAGTCACTGCGCGCGCTTCTTCTACTGCGCGTTTGATAGATGCCGCGTCATGACCGTCGATGTCGCGAATAACGTGCCAGCCGTAAGCTTCGAAACGCATTGCGGTGTCGTCGGTGAACCAGCCTTCAACGTGACCATCGATAGAAATACCGTTGTCATCGTAGAATGCAATCAGTTTACCCAGCTTCAGCGTACCCGCCAGAGAGCAAACTT  >  CP009273/3074203‑3074501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A