Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,130,516 C→A 31.1% D456Y (GAC→TAC)  gss ← fused glutathionylspermidine amidase/glutathionylspermidine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,130,5160CA31.1% 548.1 / 221.8 273D456Y (GAC→TAC) gssfused glutathionylspermidine amidase/glutathionylspermidine synthetase
Reads supporting (aligned to +/- strand):  ref base C (106/82);  new base A (45/40);  total (151/122)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 273 total aligned reads displayed.

GAGGATCGGCAGAATCGCTTTGTTGCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCC  >  CP009273/3130370‑3130664
                                                                                                                                                  |                                                                                                                                                    
gagGATCGGCAGAATCGCTTTGTTGCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCt                                                                                                                                                   >  1:2009084/1‑150 (MQ=255)
 agGATCGGCAGAATCGCTTTGTTGCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTa                                                                                                                                                  <  1:81929/150‑1 (MQ=255)
    aTCGGCAGAATCGCTTTGTTGCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACt                                                                                                                                               <  2:3190987/150‑1 (MQ=255)
     tCGGCAGAATCGCTTTGTTGCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACtt                                                                                                                                              >  1:247562/1‑150 (MQ=255)
      cGGCAGAATCGCTTTGTTGCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTc                                                                                                                                             >  2:3421595/1‑150 (MQ=255)
        gCAGAATCGCTTTGTTGCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCAc                                                                                                                                           <  1:1255092/150‑1 (MQ=255)
        gCAGAATCGCTTTGTTGCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCAc                                                                                                                                           <  1:1255179/150‑1 (MQ=255)
           gAATCGCTTTGTTGCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGaa                                                                                                                                        >  1:4415261/1‑150 (MQ=255)
            aaTCGCTTTGTTGCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAAt                                                                                                                                       >  2:4641431/1‑150 (MQ=255)
             aTCGCTTTGTTGCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGATTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATc                                                                                                                                      >  2:3242654/1‑150 (MQ=255)
                  tttGTTGCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATc                                                                                                                                 >  1:2378971/1‑150 (MQ=255)
                     gttgCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCaaa                                                                                                                              >  2:781844/1‑150 (MQ=255)
                      ttgCCGGGGATCACCGTCCACAGCTGCTCATAGACCAGCACTTCCGGTCGAAGCAATACTTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCATCAAACTCACTGTCGCTAACTTCACGAATCTGATCATAc                                                                                                                             <  1:2544519/150‑1 (MQ=255)
                      ttgCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAAc                                                                                                                             <  2:563924/150‑1 (MQ=255)
                      ttgCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAAc                                                                                                                             <  1:1335634/150‑1 (MQ=255)
                      ttgCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAAc                                                                                                                             <  2:903821/150‑1 (MQ=255)
                       tgCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAAcg                                                                                                                            >  2:1364499/1‑150 (MQ=255)
                       tgCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAAcg                                                                                                                            <  2:1490049/150‑1 (MQ=255)
                          cGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCgg                                                                                                                         >  1:1172308/1‑150 (MQ=255)
                           ggggATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAGGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGt                                                                                                                        >  2:1633048/1‑150 (MQ=255)
                           ggggATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGt                                                                                                                        >  1:885035/1‑150 (MQ=255)
                             ggATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCCGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGttt                                                                                                                      >  2:286995/1‑150 (MQ=255)
                             ggATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGttt                                                                                                                      <  2:1113324/150‑1 (MQ=255)
                               aTCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTcc                                                                                                                    >  2:1851765/1‑150 (MQ=255)
                                 cACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCTCTAACTTCACGAATCTGATCAAACGCGGTTTCCCa                                                                                                                  >  1:774850/1‑150 (MQ=255)
                                 cACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCa                                                                                                                  <  2:2460255/150‑1 (MQ=255)
                                 cACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCa                                                                                                                  >  2:457961/1‑150 (MQ=255)
                                           aGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGtt                                                                                                        >  2:1706894/1‑150 (MQ=255)
                                           aGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGtt                                                                                                        <  1:2763314/150‑1 (MQ=255)
                                             cGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGtttt                                                                                                      <  2:4626530/150‑1 (MQ=255)
                                             cGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGtttt                                                                                                      <  2:3885737/150‑1 (MQ=255)
                                              ggCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTc                                                                                                     >  1:1081113/1‑150 (MQ=255)
                                              ggCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTc                                                                                                     <  1:530604/150‑1 (MQ=255)
                                                 tCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCcac                                                                                                  >  1:2069384/1‑150 (MQ=255)
                                                       aCCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAg                                                                                            >  2:2076951/1‑150 (MQ=255)
                                                       aCCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAg                                                                                            >  2:2077263/1‑150 (MQ=255)
                                                         cAGCACTTCCGGGCGCATCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAACCGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGtt                                                                                          <  2:1420908/150‑1 (MQ=255)
                                                         cAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGtt                                                                                          >  2:3800161/1‑150 (MQ=255)
                                                          aGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCCGGTTTTCCACACGCAGTTa                                                                                         >  2:3500521/1‑150 (MQ=255)
                                                           gCACTTCCGGGCGCAGAAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTaa                                                                                        <  1:2881261/150‑1 (MQ=255)
                                                            cACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAAc                                                                                       <  2:2562644/150‑1 (MQ=255)
                                                              cTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCa                                                                                     >  1:3303504/1‑150 (MQ=255)
                                                                 ccGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTc                                                                                  <  1:244872/150‑1 (MQ=255)
                                                                 ccGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTc                                                                                  <  1:2754778/150‑1 (MQ=255)
                                                                 ccGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTc                                                                                  <  2:2111562/150‑1 (MQ=255)
                                                                 ccGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTc                                                                                  <  2:3970781/150‑1 (MQ=255)
                                                                 ccGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTc                                                                                  <  1:3630726/150‑1 (MQ=255)
                                                                  cGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCg                                                                                 >  1:3945411/1‑150 (MQ=255)
                                                                    ggCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGcc                                                                               <  2:4136670/150‑1 (MQ=255)
                                                                        cagcaATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTc                                                                           <  1:4275872/150‑1 (MQ=255)
                                                                          gcaATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTAGCCCTTccc                                                                         <  1:99009/150‑1 (MQ=255)
                                                                           caATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTcccc                                                                        <  1:215207/150‑1 (MQ=255)
                                                                              tACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTTACCAGTCGCCCTTCCCCatc                                                                     <  1:1109883/150‑1 (MQ=255)
                                                                                 gTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCatcaat                                                                  >  2:4452206/1‑150 (MQ=255)
                                                                                 gTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCatcaat                                                                  >  2:157737/1‑150 (MQ=255)
                                                                                   cGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCCCACGCAGTTAACCAGTCGCCCGGCCCCatcaatca                                                                >  1:1030318/1‑150 (MQ=255)
                                                                                      tAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGtt                                                             <  1:3257625/150‑1 (MQ=255)
                                                                                       aaGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTg                                                            >  1:1991871/1‑150 (MQ=255)
                                                                                        aGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGc                                                           >  2:4110437/1‑150 (MQ=255)
                                                                                         gACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGcc                                                          <  1:2277182/150‑1 (MQ=255)
                                                                                              aCTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGgca                                                     >  2:1019969/1‑150 (MQ=255)
                                                                                               cTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACTCAGTTAACCATTCGCCCTTCCCCATCAATCAGTTGCCCGgcag                                                    <  1:3312900/150‑1 (MQ=255)
                                                                                               cTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGgcag                                                    >  2:4122104/1‑150 (MQ=255)
                                                                                                  cGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCAt                                                 <  2:2009872/150‑1 (MQ=255)
                                                                                                   gTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATATGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAAACAGTTGCCCGGCAGCATc                                                >  1:1371920/1‑150 (MQ=255)
                                                                                                       tGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATcccag                                            >  2:3686461/1‑150 (MQ=255)
                                                                                                          ggATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATcccagccc                                         <  2:3012722/150‑1 (MQ=255)
                                                                                                           gATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATcccagccca                                        >  2:3642460/1‑150 (MQ=255)
                                                                                                            aTGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATcccagcccag                                       >  1:66948/1‑150 (MQ=255)
                                                                                                             tGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGt                                      <  2:185315/150‑1 (MQ=255)
                                                                                                              gACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGtt                                     >  2:680090/1‑150 (MQ=255)
                                                                                                              gACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGtt                                     <  1:184280/150‑1 (MQ=255)
                                                                                                              gACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTAGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGtt                                     >  1:2724833/1‑150 (MQ=255)
                                                                                                                 cGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCat                                  <  2:3086913/150‑1 (MQ=255)
                                                                                                                  ggTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatc                                 >  2:2807358/1‑150 (MQ=255)
                                                                                                                  ggTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatc                                 >  1:2675118/1‑150 (MQ=255)
                                                                                                                  ggTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTGCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatc                                 >  1:1510114/1‑150 (MQ=255)
                                                                                                                  ggTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACGTCACGAATCTGATCAAACGCGGTTTACCACGCCCCGGTTTTCCAAAAGCAGTTAAACAGTAGCCCTTCCCCATAAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatc                                 >  1:783511/1‑150 (MQ=255)
                                                                                                                   gTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatcc                                >  1:3070948/1‑150 (MQ=255)
                                                                                                                   gTACGGATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatcc                                >  2:655660/1‑150 (MQ=255)
                                                                                                                    tACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatcca                               <  1:600978/150‑1 (MQ=255)
                                                                                                                       ggATTGGCACCGCAGCAAACTCACGGTCGCTAAATTCAACAATCTGATAAAACGCGGTTTCCCACGCCCAGGTTTTACACACGCAGATAAACAGTAGCCCTTCCCCAAAAATCATATGCCCGGCAGCATCCCAGCCCAGGTCaaccaatc                            >  1:1434481/1‑150 (MQ=255)
                                                                                                                       ggATTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCatccaatc                            >  2:2055091/1‑150 (MQ=255)
                                                                                                                         aTTGGCACCGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGTTTACCCACGCCCAGGTTATCCACACGCAGTTAACCAGTCGCCCTTCCCAATCAATCAGTTCCACGGCAGCATCCCAGCCCAGTTCatccaatcca                          >  1:275278/1‑150 (MQ=255)
                                                                                                                            ggCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGc                       >  1:3816556/1‑150 (MQ=255)
                                                                                                                             gCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCa                      >  2:1330477/1‑150 (MQ=255)
                                                                                                                              cACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCaa                     <  1:3421595/150‑1 (MQ=255)
                                                                                                                                ccGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGa                   <  1:1805441/150‑1 (MQ=255)
                                                                                                                                 cGCAGCAAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGAt                  >  1:2946318/1‑150 (MQ=255)
                                                                                                                                  gcagcaAACTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATa                 <  1:1712796/150‑1 (MQ=255)
                                                                                                                                       aaaCTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATACAATCCACGCAAGATACGCGt            >  1:3154832/1‑150 (MQ=255)
                                                                                                                                        aaCTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGtt           <  1:456979/150‑1 (MQ=255)
                                                                                                                                         aCTCACGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGttt          >  2:2262921/1‑150 (MQ=255)
                                                                                                                                           tCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCa        >  2:2581135/1‑150 (MQ=255)
                                                                                                                                           tCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCa        >  2:1059376/1‑150 (MQ=255)
                                                                                                                                             aCGGTCGCTAACTTCACGAATCTGATCAAACGAGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTAATCCAATCCACGCAAGATACGCGTTTCaaa      <  2:2151313/150‑1 (MQ=255)
                                                                                                                                             aCGGTAGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCaaa      >  1:4304433/1‑150 (MQ=255)
                                                                                                                                               ggTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGc    >  1:2626655/1‑150 (MQ=255)
                                                                                                                                                gTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGcc   <  1:4481777/150‑1 (MQ=255)
                                                                                                                                                 tCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGccc  <  2:3511394/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GAGGATCGGCAGAATCGCTTTGTTGCCGGGGATCACCGTCCACAGCGGCTCAAAGACCAGCACTTCCGGGCGCAGCAATACGTCGATAAGACGCACTTCGTTTTGCGGATGACCGGTACGGATTGGCACCGCAGCAAACTCACGGTCGCTAACTTCACGAATCTGATCAAACGCGGTTTCCCACGCCCAGGTTTTCCACACGCAGTTAACCAGTCGCCCTTCCCCATCAATCAGTTGCCCGGCAGCATCCCAGCCCAGTTCATCCAATCCACGCAAGATACGCGTTTCAAAGCCC  >  CP009273/3130370‑3130664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A