Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,225,300 C→A 11.9% Q93K (CAG→AAG)  fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,225,3000CA11.9% 956.9 / 67.2 268Q93K (CAG→AAG) fadH2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base C (101/135);  new base A (10/22);  total (111/157)
Fisher's exact test for biased strand distribution p-value = 2.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 270 total aligned reads displayed.

TTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCG  >  CP009273/3225151‑3225447
                                                                                                                                                     |                                                                                                                                                   
  tATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAg                                                                                                                                                   >  2:1645140/1‑150 (MQ=255)
      ccGAAAGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACATGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGACAGCCAGATCCCACACCATCGCACAATTACCGAAGCGGTACATCAGGAAg                                                                                                                                               <  1:1790264/150‑1 (MQ=255)
       cGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATaaggaagg                                                                                                                                              <  1:4208527/150‑1 (MQ=255)
        gAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAggc                                                                                                                                             >  2:2763081/1‑150 (MQ=255)
        gAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAggc                                                                                                                                             >  2:554934/1‑150 (MQ=255)
         atcgcgCCCGTCTCGGCGTGGCGCTGATTGTCAGCGGCGGCTTTTCACCAGATTTAACAGGCGTTGGCATTGTAGGTGGCTCAATGCTCAACGACGCCTGCCAGATCCCACTCCATCGCACGATTACCGTTGCGGTACATCAGGAAggcg                                                                                                                                            <  2:688615/148‑1 (MQ=255)
          aCGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAggcgg                                                                                                                                           >  1:3763856/1‑150 (MQ=255)
           cgcgCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAggcggc                                                                                                                                          <  2:3391012/150‑1 (MQ=255)
            gcgcCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCa                                                                                                                                         <  1:851610/150‑1 (MQ=255)
                ccGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAAt                                                                                                                                     >  2:82051/1‑150 (MQ=255)
                ccGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAAt                                                                                                                                     <  2:3396128/150‑1 (MQ=255)
                 cGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATa                                                                                                                                    <  2:1031948/150‑1 (MQ=255)
                 cGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATa                                                                                                                                    <  2:2037128/150‑1 (MQ=255)
                  gTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAg                                                                                                                                   <  2:647330/150‑1 (MQ=255)
                      cGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGTGGCAAAATAGCCCt                                                                                                                               <  2:3841742/150‑1 (MQ=255)
                      cGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCt                                                                                                                               <  1:85574/150‑1 (MQ=255)
                      cGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACACCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCt                                                                                                                               >  1:541242/1‑150 (MQ=255)
                         cGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCa                                                                                                                            <  2:4316189/150‑1 (MQ=255)
                             gcgcTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAAtt                                                                                                                        <  1:3809660/150‑1 (MQ=255)
                               gcTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAAtttt                                                                                                                      <  1:2698994/150‑1 (MQ=255)
                                cTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTg                                                                                                                     <  1:1067541/150‑1 (MQ=255)
                                  gATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCa                                                                                                                   >  2:3799663/1‑150 (MQ=255)
                                   aTTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCAt                                                                                                                  >  1:4239263/1‑150 (MQ=255)
                                    ttGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATa                                                                                                                 <  2:1377079/150‑1 (MQ=255)
                                     tGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATAc                                                                                                                <  2:23224/150‑1 (MQ=255)
                                       tCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCg                                                                                                              <  2:1162661/150‑1 (MQ=255)
                                        cAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCgg                                                                                                             >  1:246424/1‑150 (MQ=255)
                                        cAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCgg                                                                                                             >  2:1921656/1‑150 (MQ=255)
                                         aGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCggg                                                                                                            <  1:499218/150‑1 (MQ=255)
                                         aGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCggg                                                                                                            >  2:2070126/1‑150 (MQ=255)
                                          gcggcgGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGgc                                                                                                           >  2:342749/1‑150 (MQ=255)
                                           cggcggCAGTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCTCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGgcg                                                                                                          <  2:857118/150‑1 (MQ=255)
                                              cggcATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTa                                                                                                       <  1:3331631/150‑1 (MQ=255)
                                              cggcATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTa                                                                                                       >  1:3693240/1‑150 (MQ=255)
                                              cggcATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTa                                                                                                       <  1:304041/150‑1 (MQ=255)
                                              cggcATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTa                                                                                                       >  1:1315445/1‑150 (MQ=255)
                                                 cATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAg                                                                                                    >  1:1357909/1‑150 (MQ=255)
                                                 cATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAg                                                                                                    <  1:4132907/150‑1 (MQ=255)
                                                    tGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTa                                                                                                 >  1:310207/1‑150 (MQ=255)
                                                    tGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTa                                                                                                 >  2:4585043/1‑150 (MQ=255)
                                                     gCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTAc                                                                                                <  2:262637/150‑1 (MQ=255)
                                                     gCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTAc                                                                                                >  1:3211939/1‑150 (MQ=255)
                                                         cAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAAc                                                                                            <  1:3200930/150‑1 (MQ=255)
                                                              ttAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCAt                                                                                       <  1:3989292/150‑1 (MQ=255)
                                                                aaCAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCt                                                                                     <  1:3714564/150‑1 (MQ=255)
                                                                aaCAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCt                                                                                     <  1:2366950/150‑1 (MQ=255)
                                                                     gCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCg                                                                                <  1:2771272/150‑1 (MQ=255)
                                                                       gTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGcc                                                                              >  2:3416339/1‑150 (MQ=255)
                                                                         tGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGcccc                                                                            <  1:4617663/150‑1 (MQ=255)
                                                                             aTGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCTCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTcc                                                                        <  2:2532959/150‑1 (MQ=255)
                                                                             aTGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTcc                                                                        <  2:4249071/150‑1 (MQ=255)
                                                                             aTGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTcc                                                                        >  1:812312/1‑150 (MQ=255)
                                                                              tGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCg                                                                       >  1:2556202/1‑150 (MQ=255)
                                                                                gAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCa                                                                     >  2:2603355/1‑150 (MQ=255)
                                                                                 aaGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCAt                                                                    <  2:4076509/150‑1 (MQ=255)
                                                                                 aaGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCAt                                                                    <  1:3601478/150‑1 (MQ=255)
                                                                                  aGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCAtt                                                                   <  1:1008396/150‑1 (MQ=255)
                                                                                  aGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCAtt                                                                   >  1:314565/1‑150 (MQ=255)
                                                                                   ggTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTg                                                                  <  2:1816087/150‑1 (MQ=255)
                                                                                       gcgcAATGCTCAACGACGCCAGCCAGATCCCACTCCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAgg                                                              <  1:2315093/150‑1 (MQ=255)
                                                                                         gcAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGcc                                                            <  1:1397674/150‑1 (MQ=255)
                                                                                         gcAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGcc                                                            <  1:1397443/150‑1 (MQ=255)
                                                                                            aTGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGccccc                                                         <  2:3701995/150‑1 (MQ=255)
                                                                                             tGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCa                                                        >  1:3939133/1‑150 (MQ=255)
                                                                                               cTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATc                                                      <  2:1329259/150‑1 (MQ=255)
                                                                                                tCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCa                                                     >  1:1407073/1‑150 (MQ=255)
                                                                                                tCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCa                                                     <  1:4320964/150‑1 (MQ=255)
                                                                                                 cAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCaa                                                    >  1:1268914/1‑150 (MQ=255)
                                                                                                     gacgCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGt                                                >  2:4356404/1‑150 (MQ=255)
                                                                                                      acgCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGtt                                               <  2:2935854/150‑1 (MQ=255)
                                                                                                        gccagccaGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTc                                             >  1:1906561/1‑150 (MQ=255)
                                                                                                        gccagccaGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTc                                             <  1:600576/150‑1 (MQ=255)
                                                                                                          cagccagATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGt                                           >  1:1224151/1‑150 (MQ=255)
                                                                                                           agccagATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTg                                          <  2:864205/150‑1 (MQ=255)
                                                                                                             ccagATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGcc                                        <  1:3489905/150‑1 (MQ=255)
                                                                                                              cagATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGccc                                       <  1:3430658/150‑1 (MQ=255)
                                                                                                                 aTCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCAt                                    >  1:1963512/1‑150 (MQ=255)
                                                                                                                 aTCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCAt                                    <  1:2291983/150‑1 (MQ=255)
                                                                                                                  tCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATg                                   >  2:3091444/1‑150 (MQ=255)
                                                                                                                  tCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATg                                   <  2:4239263/150‑1 (MQ=255)
                                                                                                                    ccACACCATCGCACCAGTACCGAAGCGGTACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAg                                 <  2:844077/150‑1 (MQ=255)
                                                                                                                     cacaCCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGt                                >  1:1688982/1‑150 (MQ=255)
                                                                                                                       cacCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTa                              >  2:88768/1‑150 (MQ=255)
                                                                                                                       cacCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTa                              >  2:4521802/1‑150 (MQ=255)
                                                                                                                        acCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCTCATGAGTTaa                             >  1:2444445/1‑150 (MQ=255)
                                                                                                                          cATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGc                           <  2:3580143/150‑1 (MQ=255)
                                                                                                                          cATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGc                           <  2:1103234/150‑1 (MQ=255)
                                                                                                                          cATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGc                           >  2:2515747/1‑150 (MQ=255)
                                                                                                                          cATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGc                           <  2:3017684/150‑1 (MQ=255)
                                                                                                                              gCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATg                       >  2:149894/1‑150 (MQ=255)
                                                                                                                                   aTTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAagag                  <  2:4424856/150‑1 (MQ=255)
                                                                                                                                   aTTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAagag                  <  1:2216896/150‑1 (MQ=255)
                                                                                                                                          aaGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGc           >  1:471241/1‑150 (MQ=255)
                                                                                                                                          aaGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGc           >  1:2249522/1‑150 (MQ=255)
                                                                                                                                           aGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCa          <  1:1817286/150‑1 (MQ=255)
                                                                                                                                           aGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCa          >  2:2085093/1‑150 (MQ=255)
                                                                                                                                              ggTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACt       <  1:3042860/150‑1 (MQ=255)
                                                                                                                                                tACATAAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGa     <  1:2084225/150‑1 (MQ=255)
                                                                                                                                                  cATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATc   <  2:3763856/150‑1 (MQ=255)
                                                                                                                                                   aTCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCg  >  2:3199092/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTTATGCCGAACGCGCCCGTCACGGCGTGGCGCTGATTGTCAGCGGCGGCATTGCACCAGATTTAACAGGCGTTGGCATGGAAGGTGGCGCAATGCTCAACGACGCCAGCCAGATCCCACACCATCGCACCATTACCGAAGCGGTACATCAGGAAGGCGGCAAAATAGCCCTGCAAATTTTGCATACCGGGCGCTACAGCTACCAACCGCATCTGGTCGCCCCGTCCGCATTGCAGGCCCCCATCAACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCG  >  CP009273/3225151‑3225447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A