Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,086,311 A→T 29.9% intergenic (‑128/+173) frvA ← / ← rhaM putative enzyme IIA component of PTS/L‑rhamnose mutarotase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,086,3110AT29.9% 519.9 / 295.3 314intergenic (‑128/+173)frvA/rhaMputative enzyme IIA component of PTS/L‑rhamnose mutarotase
Reads supporting (aligned to +/- strand):  ref base A (113/107);  new base T (33/61);  total (146/168)
Fisher's exact test for biased strand distribution p-value = 9.46e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 318 total aligned reads displayed.

GCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGG  >  CP009273/4086163‑4086459
                                                                                                                                                    |                                                                                                                                                    
  ttGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCAt                                                                                                                                                   <  1:4538950/150‑1 (MQ=255)
      aGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGGGTAATGCACATAATg                                                                                                                                               >  2:3126793/1‑150 (MQ=255)
       gTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATgg                                                                                                                                              <  2:4372383/150‑1 (MQ=255)
        tAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGt                                                                                                                                             <  2:543419/150‑1 (MQ=255)
        tAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGt                                                                                                                                             >  1:1805162/1‑150 (MQ=255)
         aaGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGtt                                                                                                                                            >  1:1077955/1‑150 (MQ=255)
          agagCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTa                                                                                                                                           >  1:2714025/1‑150 (MQ=255)
           gagCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTAt                                                                                                                                          <  1:3731926/150‑1 (MQ=255)
             gCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAAGGGTGGAGCGATTAAGTGATGAGATGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTAttt                                                                                                                                        <  2:1084254/150‑1 (MQ=255)
               tGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTtaa                                                                                                                                      >  1:422449/1‑150 (MQ=255)
                gCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTtaaa                                                                                                                                     >  2:4568896/1‑150 (MQ=255)
                  cATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTtaaata                                                                                                                                   <  2:2806192/150‑1 (MQ=255)
                     tAATCGGACTCCATAAAATATATTTTAATCACTTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAAc                                                                                                                                <  2:4332668/150‑1 (MQ=255)
                      aaTCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAAcc                                                                                                                               >  2:2344366/1‑150 (MQ=255)
                       aTCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACca                                                                                                                              >  1:3879242/1‑150 (MQ=255)
                       aTCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACca                                                                                                                              >  2:463404/1‑150 (MQ=255)
                       aTCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACca                                                                                                                              >  1:1420751/1‑150 (MQ=255)
                          ggACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACAt                                                                                                                           <  1:2614390/150‑1 (MQ=255)
                            aCTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGa                                                                                                                         <  2:1738192/150‑1 (MQ=255)
                            aCTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGa                                                                                                                         >  1:2118084/1‑150 (MQ=255)
                             cTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGaa                                                                                                                        >  2:1946844/1‑150 (MQ=255)
                                  tAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCAtt                                                                                                                   >  1:688744/1‑150 (MQ=255)
                                    aaaTATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTaa                                                                                                                 <  1:1005962/150‑1 (MQ=255)
                                     aaTATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAAt                                                                                                                <  2:3792173/150‑1 (MQ=255)
                                     aaTATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAAt                                                                                                                <  2:3422370/150‑1 (MQ=255)
                                      atatatTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATg                                                                                                               >  2:2618385/1‑150 (MQ=255)
                                      atatatTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATg                                                                                                               <  1:149378/150‑1 (MQ=255)
                                       tatatTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATgg                                                                                                              <  1:3139225/150‑1 (MQ=255)
                                       tatatTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATgg                                                                                                              >  2:4498156/1‑150 (MQ=255)
                                       tatatTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATgg                                                                                                              >  2:3549637/1‑150 (MQ=255)
                                       tatatTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATgg                                                                                                              >  2:1133842/1‑150 (MQ=255)
                                        atatTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATggg                                                                                                             <  1:788608/150‑1 (MQ=255)
                                            tttAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTAt                                                                                                         <  1:3111905/150‑1 (MQ=255)
                                             ttAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTAtt                                                                                                        >  1:1731798/1‑150 (MQ=255)
                                               aaTCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCa                                                                                                      >  1:3840240/1‑150 (MQ=255)
                                                  cAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCatta                                                                                                   <  2:1345249/150‑1 (MQ=255)
                                                   aaTTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCattat                                                                                                  <  1:2782516/150‑1 (MQ=255)
                                                     ttGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTAttt                                                                                                <  2:3444696/150‑1 (MQ=255)
                                                      tGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTAtttt                                                                                               >  2:1612616/1‑150 (MQ=255)
                                                      tGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTAtttt                                                                                               >  2:3673911/1‑150 (MQ=255)
                                                       gTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTAttttt                                                                                              >  2:301744/1‑150 (MQ=255)
                                                       gTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTAttttt                                                                                              <  1:60112/150‑1 (MQ=255)
                                                             gtggAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGAtt                                                                                        <  1:307527/150‑1 (MQ=255)
                                                              tggAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGAttt                                                                                       >  2:2831022/1‑150 (MQ=255)
                                                               ggAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTa                                                                                      <  1:463404/150‑1 (MQ=255)
                                                                gAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTAc                                                                                     <  1:136134/150‑1 (MQ=255)
                                                                    gATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTcac                                                                                 >  1:1583133/1‑150 (MQ=255)
                                                                    gATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTcac                                                                                 >  1:2737596/1‑150 (MQ=255)
                                                                        aaGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATg                                                                             <  1:3506135/150‑1 (MQ=255)
                                                                             atgaGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCa                                                                        >  2:599541/1‑150 (MQ=255)
                                                                               gagaGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAgg                                                                      <  2:446706/150‑1 (MQ=255)
                                                                               gagaGGAGATATGTCGGTAAACAATGACTATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAgg                                                                      <  2:674273/150‑1 (MQ=255)
                                                                                agagGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAggg                                                                     >  2:3109805/1‑150 (MQ=255)
                                                                                agagGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAggg                                                                     >  2:1644598/1‑150 (MQ=255)
                                                                                    gCGATATGTCGGTAAACAATGTCAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAat                                                                 <  2:73224/150‑1 (MQ=255)
                                                                                      gATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAatat                                                               >  2:666574/1‑150 (MQ=255)
                                                                                        tatGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATaa                                                             <  1:510513/150‑1 (MQ=255)
                                                                                        tatGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATaa                                                             >  1:3494434/1‑150 (MQ=255)
                                                                                         atGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAAc                                                            >  2:1532425/1‑150 (MQ=255)
                                                                                         atGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAAc                                                            >  1:710015/1‑150 (MQ=255)
                                                                                          tGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACt                                                           <  1:3637357/150‑1 (MQ=255)
                                                                                           ttCGGTAAAGTATGTGAATTTGAGACAGTGTGAAAGGTCTGATTTGCGGAGTATTGCTCATAATGGTTATTTAAATAATCCTCATTATTCTTTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCTGGTATATATATCtt                                                          <  1:4051083/149‑1 (MQ=255)
                                                                                                  aaCAATGACACTTTGAGCCAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTTATTTACTCACAATGTATCCGGGAAATATAACTTACCGGga                                                   <  2:2290600/150‑1 (MQ=255)
                                                                                                   aCAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGgag                                                  <  1:2336893/150‑1 (MQ=255)
                                                                                                   aCAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGgag                                                  <  1:4570769/150‑1 (MQ=255)
                                                                                                    cAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGgaga                                                 >  1:2946677/1‑150 (MQ=255)
                                                                                                        gACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTa                                             >  2:2479500/1‑150 (MQ=255)
                                                                                                           aaTTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAata                                          <  1:2673432/150‑1 (MQ=255)
                                                                                                            aTTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAatat                                         <  1:1206806/150‑1 (MQ=255)
                                                                                                             tttGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATg                                        <  1:4443617/150‑1 (MQ=255)
                                                                                                               tGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGtt                                      <  1:326836/150‑1 (MQ=255)
                                                                                                                gagaCAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGttt                                     <  1:2355780/150‑1 (MQ=255)
                                                                                                                gagaCAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGttt                                     >  1:2998088/1‑150 (MQ=255)
                                                                                                                   aCAGAGTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAAt                                  >  2:127352/1‑150 (MQ=255)
                                                                                                                       agTGAAAGGTCAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAAttttt                              >  2:1838192/1‑150 (MQ=255)
                                                                                                                        gTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTc                             <  1:2223559/150‑1 (MQ=255)
                                                                                                                                 cAGATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGaaa                    <  1:244290/150‑1 (MQ=255)
                                                                                                                                 cAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGaaa                    >  1:3594932/1‑150 (MQ=255)
                                                                                                                                  aGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAAt                   >  2:3349342/1‑150 (MQ=255)
                                                                                                                                  aGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAAt                   <  2:1235185/150‑1 (MQ=255)
                                                                                                                                  aGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAAt                   >  2:1018746/1‑150 (MQ=255)
                                                                                                                                   gATTTGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAAtt                  <  2:2192455/150‑1 (MQ=255)
                                                                                                                                    aTTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAAttt                 <  2:83393/150‑1 (MQ=255)
                                                                                                                                     tttGCGGAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTa                >  2:2641297/1‑150 (MQ=255)
                                                                                                                                     tttGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGATATGTAATATGTTTAATTTTTCAAATCGAAATTTa                <  2:2118084/150‑1 (MQ=255)
                                                                                                                                     tttGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTa                <  1:2005373/150‑1 (MQ=255)
                                                                                                                                      ttGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTTATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTaa               >  2:961741/1‑150 (MQ=255)
                                                                                                                                       tGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTaaa              >  2:3056284/1‑150 (MQ=255)
                                                                                                                                         cGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAat            >  1:1844451/1‑150 (MQ=255)
                                                                                                                                          ggAGTAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAata           >  1:4163610/1‑150 (MQ=255)
                                                                                                                                           gAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAatat          <  2:4517426/150‑1 (MQ=255)
                                                                                                                                           gAGTAATGAACATAATTGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGTGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAatat          <  2:1547416/150‑1 (MQ=255)
                                                                                                                                            aGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATAtt         >  1:2851821/1‑150 (MQ=255)
                                                                                                                                              tAATGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATtgt       <  2:2225784/150‑1 (MQ=255)
                                                                                                                                               aaTGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATtgtg      <  1:1612616/150‑1 (MQ=255)
                                                                                                                                               aaTGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTTATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAATTCGAAATTTAAAATATtgtg      >  2:422109/1‑150 (MQ=255)
                                                                                                                                               aaTGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTTATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATtgtg      >  2:2773284/1‑150 (MQ=255)
                                                                                                                                               aaTGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATtgtg      <  2:2950981/150‑1 (MQ=255)
                                                                                                                                                aTGCTCATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGc     >  2:4085182/1‑150 (MQ=255)
                                                                                                                                                 tGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGcc    <  1:476604/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATGCACATAATGGTTATTTAAATAAACCACATGAATCATTAATGGGTTATTCATTATTTTTGTGATTTACTCACAATGTATCAGGGAAATATAACTTACCGGGAGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGG  >  CP009273/4086163‑4086459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A