Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F224 I0 R1 1457 284.0 9290224 95.7% 8890744 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,092,998 G→T 39.9% A83A (GCG→GCT)  yiiM → 6‑N‑hydroxylaminopurine resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,092,9980GT39.9% 371.5 / 295.5 275A83A (GCG→GCT) yiiM6‑N‑hydroxylaminopurine resistance protein
Reads supporting (aligned to +/- strand):  ref base G (74/91);  new base T (56/54);  total (130/145)
Fisher's exact test for biased strand distribution p-value = 3.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

GACGGAGCTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATCAGCGATATA  >  CP009273/4092851‑4093145
                                                                                                                                                   |                                                                                                                                                    
 aCGGAGCTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCtttt                                                                                                                                                   <  2:336138/150‑1 (MQ=255)
 aCGGAGCTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGttt                                                                                                                                                   >  2:795514/1‑150 (MQ=255)
  cGGAGCTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGTGCGCGGGAATTTCCTGAACAGGCTGAGATGTTTGTTGCGCCTGCGTTTg                                                                                                                                                  >  2:1289207/1‑150 (MQ=255)
   ggAGCTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTgg                                                                                                                                                 >  2:2710361/1‑150 (MQ=255)
   ggAGCTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTgg                                                                                                                                                 >  1:3318642/1‑150 (MQ=255)
   ggAGCTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTgg                                                                                                                                                 >  2:3793820/1‑150 (MQ=255)
    gAGCTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGt                                                                                                                                                >  1:3399457/1‑150 (MQ=255)
       cTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGaa                                                                                                                                             <  2:704618/150‑1 (MQ=255)
       cTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGaa                                                                                                                                             >  1:2774822/1‑150 (MQ=255)
       cTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGaa                                                                                                                                             >  2:1734923/1‑150 (MQ=255)
        tGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGaaa                                                                                                                                            >  2:3047295/1‑150 (MQ=255)
        tGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGaaa                                                                                                                                            >  1:3092139/1‑150 (MQ=255)
             cTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACctc                                                                                                                                       <  1:2812241/150‑1 (MQ=255)
               ggAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCtctc                                                                                                                                     >  2:738024/1‑150 (MQ=255)
                gAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCa                                                                                                                                    <  1:2623998/150‑1 (MQ=255)
                 aaGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCaa                                                                                                                                   <  2:3282206/150‑1 (MQ=255)
                   ggTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAAcc                                                                                                                                 <  1:3082241/150‑1 (MQ=255)
                      gACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGAc                                                                                                                              >  1:3521412/1‑150 (MQ=255)
                       aCGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCATCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCACTCAACCGACg                                                                                                                             >  1:1992925/1‑150 (MQ=255)
                        cGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACgg                                                                                                                            >  2:2558040/1‑150 (MQ=255)
                        cGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACgg                                                                                                                            >  2:1073006/1‑150 (MQ=255)
                         gAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGc                                                                                                                           >  1:4533103/1‑150 (MQ=255)
                          aGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGcc                                                                                                                          <  1:2415140/150‑1 (MQ=255)
                          aGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGcc                                                                                                                          >  1:207228/1‑150 (MQ=255)
                           gCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCt                                                                                                                         >  1:2959780/1‑150 (MQ=255)
                            cAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTg                                                                                                                        >  1:947536/1‑150 (MQ=255)
                             aGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGa                                                                                                                       >  1:4385195/1‑150 (MQ=255)
                             aGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGa                                                                                                                       <  2:3959755/150‑1 (MQ=255)
                                cGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACgg                                                                                                                    >  2:421664/1‑150 (MQ=255)
                                 ggAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGa                                                                                                                   <  1:2640528/150‑1 (MQ=255)
                                 ggAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGa                                                                                                                   <  1:3282839/150‑1 (MQ=255)
                                   agaAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGaaa                                                                                                                 >  1:1277273/1‑150 (MQ=255)
                                           gTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGtt                                                                                                         <  1:1704725/150‑1 (MQ=255)
                                             tCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑ta                                                                                                      <  1:132631/150‑1 (MQ=255)
                                             tCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑ta                                                                                                      <  1:133600/150‑1 (MQ=255)
                                             tCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑ta                                                                                                      <  1:1500412/150‑1 (MQ=255)
                                             tCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑ta                                                                                                      <  1:3688964/150‑1 (MQ=255)
                                             tCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑ta                                                                                                      <  1:581170/150‑1 (MQ=255)
                                               aCGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑tata                                                                                                    <  2:1393031/150‑1 (MQ=255)
                                                 ggcggGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATg                                                                                                  <  1:1201329/150‑1 (MQ=255)
                                                 ggcggGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATg                                                                                                  >  1:636953/1‑150 (MQ=255)
                                                  gcggGCCAGTCAGTGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATgg                                                                                                 <  1:1858421/150‑1 (MQ=255)
                                                    gggCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGc                                                                                               >  2:1512806/1‑150 (MQ=255)
                                                    gggCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGc                                                                                               >  2:1221260/1‑150 (MQ=255)
                                                     ggCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCg                                                                                              >  2:2886592/1‑150 (MQ=255)
                                                       ccAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGat                                                                                            >  1:3352502/1‑150 (MQ=255)
                                                         agacagaGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGatat                                                                                          <  1:1064043/150‑1 (MQ=255)
                                                            cagaGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATAtttt                                                                                       >  1:2700307/1‑150 (MQ=255)
                                                             agagCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTc                                                                                      >  2:2786243/1‑150 (MQ=255)
                                                               agCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCg                                                                                    <  1:2833523/150‑1 (MQ=255)
                                                                 cgcTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCt                                                                                  >  2:3657047/1‑150 (MQ=255)
                                                                    tgtgTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTggg                                                                               <  2:4050602/150‑1 (MQ=255)
                                                                     gtgtCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTgggg                                                                              <  1:836349/150‑1 (MQ=255)
                                                                       gtCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGgag                                                                            >  1:1452285/1‑150 (MQ=255)
                                                                          aTTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAgg                                                                         >  2:3059386/1‑150 (MQ=255)
                                                                          aTTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAgg                                                                         <  2:1857356/150‑1 (MQ=255)
                                                                           ttATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGc                                                                        >  1:717412/1‑150 (MQ=255)
                                                                           ttATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCGGGGGAGAGGc                                                                        >  1:4121824/1‑150 (MQ=255)
                                                                            tATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGGTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCa                                                                       >  2:918722/1‑150 (MQ=255)
                                                                               ccTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTa                                                                    >  2:3694001/1‑150 (MQ=255)
                                                                                   gTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTc                                                                >  2:4555967/1‑150 (MQ=255)
                                                                                   gTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTc                                                                >  1:4022149/1‑150 (MQ=255)
                                                                                    tGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCa                                                               <  1:3950260/150‑1 (MQ=255)
                                                                                      aGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAgg                                                             <  2:4076172/150‑1 (MQ=255)
                                                                                      aGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAgg                                                             <  1:2926092/150‑1 (MQ=255)
                                                                                         aTTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTca                                                          <  1:4214999/150‑1 (MQ=255)
                                                                                         aTTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTca                                                          >  1:214724/1‑150 (MQ=255)
                                                                                         aTTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTca                                                          <  1:1658852/150‑1 (MQ=255)
                                                                                            aTCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagcc                                                       <  1:3468878/150‑1 (MQ=255)
                                                                                             tctcTACTGGGCGCGGGAATGTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagcca                                                      <  2:3230514/150‑1 (MQ=255)
                                                                                              ctctACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTcagccag                                                     <  1:3584127/150‑1 (MQ=255)
                                                                                                ctACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCagccagcc                                                   >  1:2977696/1‑150 (MQ=255)
                                                                                                    tGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCgcgc                                               >  2:3457194/1‑150 (MQ=255)
                                                                                                    tGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCgcgc                                               >  2:1879655/1‑150 (MQ=255)
                                                                                                      ggCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTc                                             <  2:1801028/150‑1 (MQ=255)
                                                                                                         gcgGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGcc                                          >  1:937005/1‑150 (MQ=255)
                                                                                                           gggAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCtt                                        >  1:380127/1‑150 (MQ=255)
                                                                                                           gggAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCtt                                        >  2:3719656/1‑150 (MQ=255)
                                                                                                            ggAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTg                                       <  1:795514/150‑1 (MQ=255)
                                                                                                            ggAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTg                                       <  2:207111/150‑1 (MQ=255)
                                                                                                              aaTTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCt                                     <  2:3406887/150‑1 (MQ=255)
                                                                                                                tttCCGGCACAGGCGGCGTTGTTTGTTGCGCCTGCGTTTTGTGAAAACCTCACAACCGACGGCCTGCCGGAAAGTAATGTTATATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCta                                    <  2:3356547/150‑1 (MQ=255)
                                                                                                                 ttCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCtata                                  <  1:2642047/150‑1 (MQ=255)
                                                                                                                 ttCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCtata                                  <  1:4424666/150‑1 (MQ=255)
                                                                                                                  tCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATaa                                 <  2:1241912/150‑1 (MQ=255)
                                                                                                                  tCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATaa                                 <  2:522720/150‑1 (MQ=255)
                                                                                                                    cGGAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAAACGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAAc                               <  1:3669365/150‑1 (MQ=255)
                                                                                                                      gAACAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTc                             >  1:107755/1‑150 (MQ=255)
                                                                                                                       aaCAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCa                            >  1:4024632/1‑150 (MQ=255)
                                                                                                                        aCAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCaa                           >  2:125071/1‑150 (MQ=255)
                                                                                                                         cAGGCGGAGTTGTTTGTTGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCCATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAAt                          >  2:908575/1‑150 (MQ=255)
                                                                                                                            gCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTAc                       <  2:1374495/150‑1 (MQ=255)
                                                                                                                              ggAGTTGTTTGTTGCGCCTGCTTTTGGTTAAAACCTCTAAACCGACGGCCTGACGGAAAGTAATTTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCa                     >  2:2737620/1‑150 (MQ=255)
                                                                                                                              ggAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCa                     <  1:4451712/150‑1 (MQ=255)
                                                                                                                                 gttgttTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCTGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACAAttt                  >  2:2504586/1‑150 (MQ=255)
                                                                                                                                   tgtttgttGCGCCTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTg                <  2:4155380/150‑1 (MQ=255)
                                                                                                                                            cgcCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATCAGCg       >  2:4346042/1‑150 (MQ=255)
                                                                                                                                              ccTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATCAGCGat     <  1:1777615/150‑1 (MQ=255)
                                                                                                                                              ccTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATCAGCGat     <  2:2959780/150‑1 (MQ=255)
                                                                                                                                               cTGCTTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATCAGCGata    >  2:4264093/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GACGGAGCTGGGGCTGGAAGGTGACGAGCAGGCGGAGAAAAAGGTTCACGGCGGGCCAGACAGAGCGCTGTGTCATTATCCTCGTGAGCATTATCTCTACTGGGCGCGGGAATTTCCGGAACAGGCGGAGTTGTTTGTTGCGCCTGCGTTTGGTGAAAACCTCTCAACCGACGGCCTGACGGAAAGTAATGTT‑TATATGGGCGATATTTTCCGCTGGGGAGAGGCATTAATTCAGGTCAGCCAGCCGCGCTCGCCTTGCTATAAACTCAATTACCATTTTGATATCAGCGATATA  >  CP009273/4092851‑4093145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A