Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F54 I0 R1 640 319.5 10493376 95.3% 10000187 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 579,710 G→A 24.4% A192T (GCA→ACA)  appY → global transcriptional activator; DLP12 prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273579,7100GA24.4% 748.4 / 256.2 363A192T (GCA→ACA) appYglobal transcriptional activator; DLP12 prophage
Reads supporting (aligned to +/- strand):  ref base G (133/140);  new base A (58/30);  total (192/171)
Fisher's exact test for biased strand distribution p-value = 6.71e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

Only 100 of 365 total aligned reads displayed.

CTGGAATAATCAGTTTTAATATTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACA  >  CP009273/579561‑579859
                                                                                                                                                     |                                                                                                                                                     
 tGGAATAATCAGTTTTAATATTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGc                                                                                                                                                      >  1:703232/1‑150 (MQ=255)
  ggAATAATCAGTTTTAATATTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATACa                                                                                                                                                     <  2:2910902/150‑1 (MQ=255)
    aataatCAGTTTTAATATTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCaaa                                                                                                                                                   >  1:4081665/1‑150 (MQ=255)
    aataatCAGTTTTAATATTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCaaa                                                                                                                                                   <  1:1669865/150‑1 (MQ=255)
       aatCAGTTTTAATATTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCaaaaaa                                                                                                                                                <  1:4085743/150‑1 (MQ=255)
         tCAGTTTTAATATTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATACAAAAAAAc                                                                                                                                              <  1:631775/150‑1 (MQ=255)
          cAGTTTTAATATTGAGCGGCAATGTCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACt                                                                                                                                             <  1:4793388/150‑1 (MQ=255)
          cAGTTTTAATATTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACt                                                                                                                                             <  1:4809187/150‑1 (MQ=255)
            gTTTTAATATTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCa                                                                                                                                           <  1:386440/150‑1 (MQ=255)
            gTTTTAATATTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCa                                                                                                                                           <  1:1548586/150‑1 (MQ=255)
             ttttAATATTGAGCGGCAATTGCATTTAAAAGATATTGCGTAATTTATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAATTGATGAATGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCAt                                                                                                                                          <  2:590902/150‑1 (MQ=255)
             ttttAATATTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCAt                                                                                                                                          <  1:4417699/150‑1 (MQ=255)
               ttAATATTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATaa                                                                                                                                        >  2:4376166/1‑150 (MQ=255)
                tAATATTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAAc                                                                                                                                       <  2:2521966/150‑1 (MQ=255)
                  atatTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACtt                                                                                                                                     <  2:2380844/150‑1 (MQ=255)
                  atatTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACtt                                                                                                                                     <  1:3138067/150‑1 (MQ=255)
                   tagtGAGCGGCAATGTCATTTAAAAGATATTGCGGAATTGATTTATACTAGTGAAAGTTTAATAAAAAAAAGATTAAGTGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATACAAAAAAACTCATAACTTc                                                                                                                                    <  2:5044810/147‑1 (MQ=255)
                    atTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATACAAAAAAACTCATAACTTCa                                                                                                                                   <  2:2031123/150‑1 (MQ=255)
                       gAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAAc                                                                                                                                <  1:389304/150‑1 (MQ=255)
                        aGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAAct                                                                                                                               <  1:2772928/150‑1 (MQ=255)
                        aGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAAct                                                                                                                               >  2:3893928/1‑150 (MQ=255)
                        aGCGGAAATGGCATTTAATAGATATTGCGGAATTGATTTATACTAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAAct                                                                                                                               <  2:3791237/150‑1 (MQ=255)
                              aaTGTCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTAtt                                                                                                                         <  2:414230/150‑1 (MQ=255)
                                 ggCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTa                                                                                                                      >  1:615355/1‑150 (MQ=255)
                                   cATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATACAAAAAAACTCATAAATTCAAACTCTTATTATATc                                                                                                                    >  2:463560/1‑150 (MQ=255)
                                    aTTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCa                                                                                                                   >  1:3482832/1‑150 (MQ=255)
                                            gATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTa                                                                                                           <  2:4693294/150‑1 (MQ=255)
                                              tatTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGc                                                                                                         <  2:805134/150‑1 (MQ=255)
                                                   cGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGa                                                                                                    >  2:4905567/1‑150 (MQ=255)
                                                    ggAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGaa                                                                                                   >  2:4570476/1‑150 (MQ=255)
                                                     gAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGaca                                                                                                  >  2:3335128/1‑148 (MQ=255)
                                                      aaTTGATTTAGACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAAt                                                                                                 <  1:750343/150‑1 (MQ=255)
                                                       aTTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAAtg                                                                                                >  1:1104686/1‑150 (MQ=255)
                                                           aTTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGc                                                                                            <  2:4235878/150‑1 (MQ=255)
                                                              tataCGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCtat                                                                                         <  1:2495724/150‑1 (MQ=255)
                                                               ataCGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCtata                                                                                        <  2:3399632/150‑1 (MQ=255)
                                                               ataCGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCtata                                                                                        <  2:3508545/150‑1 (MQ=255)
                                                                  cGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTCTAACa                                                                                     >  2:3837249/1‑150 (MQ=255)
                                                                        aaaGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAGTATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACtt                                                                               >  1:1604261/1‑150 (MQ=255)
                                                                        aaaGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACtt                                                                               >  1:4215459/1‑150 (MQ=255)
                                                                          attataataaAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttca                                                                             <  2:3689274/146‑1 (MQ=255)
                                                                          aGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttca                                                                             >  1:3944745/1‑150 (MQ=255)
                                                                           gTTTAATAAAAAAAAGATTAAGTGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcat                                                                            <  2:361382/150‑1 (MQ=255)
                                                                           gTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcat                                                                            <  2:931881/150‑1 (MQ=255)
                                                                               aataaAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatattt                                                                        >  1:2139122/1‑150 (MQ=255)
                                                                                ataaAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTATATAAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttc                                                                       >  2:3567958/1‑150 (MQ=255)
                                                                                ataaAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttc                                                                       <  2:1789971/150‑1 (MQ=255)
                                                                                  aaaaaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttcat                                                                     <  1:4591401/150‑1 (MQ=255)
                                                                                  aaaaaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttcat                                                                     <  2:1735102/150‑1 (MQ=255)
                                                                                   aaaaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttcata                                                                    >  1:770193/1‑150 (MQ=255)
                                                                                   aaaaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttcata                                                                    >  2:677896/1‑150 (MQ=255)
                                                                                    aaaaaaGATTAAGGGATGAAGGAACGTCATTTACTGACATATTGAGAGATACTAGGATGAGGTATGCAAAAACACTCATAACTTCAACCTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGTCTATAACAGTCCttcattgttcatct                                                                   >  2:3198862/1‑148 (MQ=255)
                                                                                    aaaaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACttcatatttcatat                                                                   <  1:2631005/150‑1 (MQ=255)
                                                                                      aaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATAtgt                                                                 <  1:377147/150‑1 (MQ=255)
                                                                                      aaaaGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATAtgt                                                                 <  2:2387632/150‑1 (MQ=255)
                                                                                          gATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCAt                                                             <  2:2151189/150‑1 (MQ=255)
                                                                                           aTTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCAtt                                                            >  1:4371756/1‑150 (MQ=255)
                                                                                           aTTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCAtt                                                            >  1:2545880/1‑150 (MQ=255)
                                                                                           aTTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCAtt                                                            >  2:3059079/1‑150 (MQ=255)
                                                                                            ttAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCAttt                                                           <  1:955136/150‑1 (MQ=255)
                                                                                               aGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTaaa                                                        <  2:2626255/150‑1 (MQ=255)
                                                                                               aGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTaaa                                                        >  1:5020261/1‑150 (MQ=255)
                                                                                                   aTGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGatt                                                    >  1:1761230/1‑150 (MQ=255)
                                                                                                   aTGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGatt                                                    >  2:4759179/1‑150 (MQ=255)
                                                                                                     gAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGattat                                                  >  2:3089222/1‑150 (MQ=255)
                                                                                                     gAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGattat                                                  >  1:367835/1‑150 (MQ=255)
                                                                                                     gAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGattat                                                  >  1:2275018/1‑150 (MQ=255)
                                                                                                      aaGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGattatt                                                 >  1:3064118/1‑150 (MQ=255)
                                                                                                        ggAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATtattat                                               <  1:4504291/150‑1 (MQ=255)
                                                                                                        ggAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATtattat                                               <  1:1064555/150‑1 (MQ=255)
                                                                                                         gAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATg                                              >  1:1461725/1‑150 (MQ=255)
                                                                                                          aaCGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGTTTATTCTgg                                             >  2:2871647/1‑150 (MQ=255)
                                                                                                          aaCGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATgg                                             >  2:1504669/1‑150 (MQ=255)
                                                                                                          aaCGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATgg                                             >  1:464690/1‑150 (MQ=255)
                                                                                                           aCGTCATTTACTGAAATATTGAGAGAGACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGgt                                            <  2:4199982/150‑1 (MQ=255)
                                                                                                            cGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGgtg                                           <  1:4152942/150‑1 (MQ=255)
                                                                                                               cATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCa                                        >  1:5118305/1‑150 (MQ=255)
                                                                                                                aTTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCAc                                       >  1:4847702/1‑150 (MQ=255)
                                                                                                                aTTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTATTATTCTATCAATGTCGTAGACCAGAAATGTGGCTATAACAGAACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCaa                                       >  1:2249103/1‑149 (MQ=255)
                                                                                                                   tACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGcc                                    >  1:2086743/1‑150 (MQ=255)
                                                                                                                     cTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCAt                                  <  1:846694/150‑1 (MQ=255)
                                                                                                                      tGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCAtc                                 <  1:333179/150‑1 (MQ=255)
                                                                                                                      tGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCAtc                                 <  1:2971298/150‑1 (MQ=255)
                                                                                                                       gAATTATTGAGAGATACTAGGATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCAtct                                <  1:1152191/150‑1 (MQ=255)
                                                                                                                        aaaTATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCAgctc                               >  2:2738979/1‑150 (MQ=255)
                                                                                                                         aaTATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTAATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCa                              >  2:1061327/1‑150 (MQ=255)
                                                                                                                           tatTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCatt                            >  2:1603214/1‑150 (MQ=255)
                                                                                                                            atTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCatta                           >  2:513062/1‑150 (MQ=255)
                                                                                                                             ttGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCattat                          <  1:1271073/150‑1 (MQ=255)
                                                                                                                               gagagaTACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTAttt                        >  1:1530020/1‑150 (MQ=255)
                                                                                                                               gagagaTACTAGGATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTCttt                        >  1:2294564/1‑150 (MQ=255)
                                                                                                                                agagaTACTAGGATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTAtttt                       >  1:4885161/1‑150 (MQ=255)
                                                                                                                                     tACTAGGATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGAGTATTATGGTGTCACGCCATCTCATTATTTTGAGaa                  >  1:2400872/1‑150 (MQ=255)
                                                                                                                                         aGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAaata              <  2:121419/150‑1 (MQ=255)
                                                                                                                                         aGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATAGTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAaata              >  2:2810046/1‑150 (MQ=255)
                                                                                                                                         aGGATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAaata              >  2:4023702/1‑150 (MQ=255)
                                                                                                                                          ggATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAaataa             >  2:1279688/1‑150 (MQ=255)
                                                                                                                                          ggATGAGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAaataa             <  1:3754544/150‑1 (MQ=255)
                                                                                                                                               aGGTATACAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGc        <  1:2819051/150‑1 (MQ=255)
                                                                                                                                                   aTGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTca    >  1:2181126/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGGAATAATCAGTTTTAATATTGAGCGGCAATGGCATTTAAAAGATATTGCGGAATTGATTTATACGAGTGAAAGTTTAATAAAAAAAAGATTAAGGGATGAAGGAACGTCATTTACTGAAATATTGAGAGATACTAGGATGAGGTATGCAAAAAAACTCATAACTTCAAACTCTTATTCTATCAATGTCGTAGCCCAGAAATGTGGCTATAACAGTACTTCATATTTCATATGTGCATTTAAAGATTATTATGGTGTCACGCCATCTCATTATTTTGAGAAAATAATCGGCGTCACA  >  CP009273/579561‑579859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A