Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F54 I0 R1 640 319.5 10493376 95.3% 10000187 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,078,521 C→A 27.1% V162V (GTG→GTT)  yeeE ← UPF0394 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,078,5210CA27.1% 545.7 / 200.1 265V162V (GTG→GTT) yeeEUPF0394 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (102/91);  new base A (31/41);  total (133/132)
Fisher's exact test for biased strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 272 total aligned reads displayed.

AGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAG  >  CP009273/2078372‑2078670
                                                                                                                                                     |                                                                                                                                                     
 gcgcAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCa                                                                                                                                                      <  1:3630989/150‑1 (MQ=255)
  cgcAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCAc                                                                                                                                                     <  2:2812604/150‑1 (MQ=255)
   gcAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCAcc                                                                                                                                                    <  1:1650116/150‑1 (MQ=255)
     aaTCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAg                                                                                                                                                  >  2:4924656/1‑150 (MQ=255)
           aCCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATCTGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTATTTCATCACCACCCAGAGCGTTATCACCAGCAAAAc                                                                                                                                            <  2:3343923/150‑1 (MQ=255)
           aCCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAAc                                                                                                                                            <  1:3027470/150‑1 (MQ=255)
           aCCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAAc                                                                                                                                            <  2:1815679/150‑1 (MQ=255)
           aCCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTATTTCATCACCACCCAGAGCGTTATCACCAGCAAAAc                                                                                                                                            <  2:718707/150‑1 (MQ=255)
                tGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGcaa                                                                                                                                       >  1:2780318/1‑150 (MQ=255)
                tGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGcaa                                                                                                                                       <  2:2228156/150‑1 (MQ=255)
                  aGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGcaacc                                                                                                                                     <  1:4014531/150‑1 (MQ=255)
                     actgCTGTTACAAAGGGATGCCAGCGTTTTTAAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGcaaccaac                                                                                                                                  <  2:434116/147‑1 (MQ=255)
                       aGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACgg                                                                                                                                >  2:2362385/1‑150 (MQ=255)
                         cTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGcc                                                                                                                              >  2:773000/1‑150 (MQ=255)
                             gACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCcacac                                                                                                                          >  2:3451806/1‑150 (MQ=255)
                             gACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCcacac                                                                                                                          >  2:5108206/1‑150 (MQ=255)
                              aCAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCcacaca                                                                                                                         <  1:1549579/150‑1 (MQ=255)
                               cAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAg                                                                                                                        <  1:2459124/150‑1 (MQ=255)
                                 aaGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGAc                                                                                                                      >  1:4237709/1‑150 (MQ=255)
                                  aGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACa                                                                                                                     <  1:2492605/150‑1 (MQ=255)
                                   gggATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACaa                                                                                                                    <  2:3752767/150‑1 (MQ=255)
                                      aTGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAAtt                                                                                                                 >  1:1834976/1‑150 (MQ=255)
                                        gCCAGCGTTTTTCAAACATAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTaa                                                                                                               <  1:5137945/150‑1 (MQ=255)
                                        gCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTaa                                                                                                               <  1:2546253/150‑1 (MQ=255)
                                         ccAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTaaa                                                                                                              <  1:815938/150‑1 (MQ=255)
                                         ccAGCGTTTTTCAAACAGAATAGGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTaaa                                                                                                              <  2:4709461/150‑1 (MQ=255)
                                           aGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAACAg                                                                                                            >  1:4980017/1‑150 (MQ=255)
                                              gTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGtct                                                                                                         >  2:4281160/1‑150 (MQ=255)
                                              gTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGtcg                                                                                                         <  2:4156212/150‑2 (MQ=255)
                                                ttttCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCa                                                                                                       >  1:1027506/1‑150 (MQ=255)
                                                 tttCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAg                                                                                                      >  1:2223617/1‑150 (MQ=255)
                                                  ttCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGc                                                                                                     <  1:3352438/150‑1 (MQ=255)
                                                   tCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCa                                                                                                    >  1:3454708/1‑150 (MQ=255)
                                                    cAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCaa                                                                                                   <  1:4553491/150‑1 (MQ=255)
                                                     aaaCAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAAt                                                                                                  >  1:1314356/1‑150 (MQ=255)
                                                       aCAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATag                                                                                                >  1:4147988/1‑150 (MQ=255)
                                                             tatGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTAt                                                                                          <  1:4714016/150‑1 (MQ=255)
                                                              atGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATg                                                                                         >  1:3815566/1‑150 (MQ=255)
                                                                 aGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTc                                                                                      >  1:2048441/1‑150 (MQ=255)
                                                                    gATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGt                                                                                   >  1:1191122/1‑150 (MQ=255)
                                                                    gATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGt                                                                                   >  1:3807079/1‑150 (MQ=255)
                                                                     aTCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATCGAGTTATGTTCAGTa                                                                                  >  2:1531595/1‑150 (MQ=255)
                                                                     aTCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTa                                                                                  <  1:2269360/150‑1 (MQ=255)
                                                                      tCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTAc                                                                                 >  1:1681366/1‑150 (MQ=255)
                                                                      tCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTAc                                                                                 >  1:598359/1‑150 (MQ=255)
                                                                           gTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGATCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAg                                                                            <  2:2001238/150‑1 (MQ=255)
                                                                           gTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAg                                                                            <  1:758901/150‑1 (MQ=255)
                                                                              cggcgcggcgGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGtgc                                                                         >  2:4127843/1‑150 (MQ=255)
                                                                               ggcgcggcgGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGtgct                                                                        >  1:2194725/1‑150 (MQ=255)
                                                                               ggcgcggcgGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGtgct                                                                        <  2:4853476/150‑1 (MQ=255)
                                                                                gcgcggcgGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGtgctg                                                                       >  2:497634/1‑150 (MQ=255)
                                                                                gcgcggcgGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGtgctg                                                                       <  1:545594/150‑1 (MQ=255)
                                                                                       ggTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTATCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGtt                                                                >  2:160716/1‑150 (MQ=255)
                                                                                          aaGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGa                                                             >  2:4784039/1‑150 (MQ=255)
                                                                                          aaGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGa                                                             >  2:4533473/1‑150 (MQ=255)
                                                                                           aGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGAt                                                            >  2:1039803/1‑150 (MQ=255)
                                                                                           aGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGAt                                                            <  1:287153/150‑1 (MQ=255)
                                                                                            ggTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGAtt                                                           <  1:1688916/150‑1 (MQ=255)
                                                                                               cgcgACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTaa                                                        >  1:3552894/1‑150 (MQ=255)
                                                                                                   aCTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTAAGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCa                                                    >  2:899432/1‑150 (MQ=255)
                                                                                                    cTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCAc                                                   >  1:4963594/1‑150 (MQ=255)
                                                                                                     tttGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACt                                                  <  2:1721078/150‑1 (MQ=255)
                                                                                                     tttGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACt                                                  <  1:4090435/150‑1 (MQ=255)
                                                                                                     tttGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACt                                                  <  1:3663666/150‑1 (MQ=255)
                                                                                                       tGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAATCCACTgt                                                >  2:2978307/1‑149 (MQ=255)
                                                                                                          gTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCAt                                             <  1:1378609/150‑1 (MQ=255)
                                                                                                           ttttGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCAtg                                            <  1:2947270/150‑1 (MQ=255)
                                                                                                           ttttGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCAtg                                            <  1:2634130/150‑1 (MQ=255)
                                                                                                              tGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTgg                                         <  2:3375008/150‑1 (MQ=255)
                                                                                                               ggCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGga                                        <  1:1401649/150‑1 (MQ=255)
                                                                                                               ggCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGga                                        >  2:5044490/1‑150 (MQ=255)
                                                                                                               ggCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGga                                        >  1:1944801/1‑150 (MQ=255)
                                                                                                                gCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGgag                                       <  2:3012157/150‑1 (MQ=255)
                                                                                                                 cttcttCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGgaga                                      >  1:2093098/1‑150 (MQ=255)
                                                                                                                  ttcttcAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGaa                                     <  1:891956/150‑1 (MQ=255)
                                                                                                                  ttcttcAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGaa                                     <  1:4486884/150‑1 (MQ=255)
                                                                                                                   tcttcAACTCTTTCATCACCACCCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAAc                                    >  1:2473407/1‑150 (MQ=255)
                                                                                                                          ctctTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCAc                             <  2:5178185/150‑1 (MQ=255)
                                                                                                                                catcaCCACCCAGGGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACt                       <  1:137773/150‑1 (MQ=255)
                                                                                                                                catcaCCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACt                       >  2:2440040/1‑150 (MQ=255)
                                                                                                                                 atcaCCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCAcac                      >  1:4024133/1‑148 (MQ=255)
                                                                                                                                   caccacCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAATGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACtcat                    <  2:4415702/150‑1 (MQ=255)
                                                                                                                                   caccacCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACtcat                    >  2:406346/1‑150 (MQ=255)
                                                                                                                                   caccacCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACtcat                    <  1:660107/150‑1 (MQ=255)
                                                                                                                                     ccaccCAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACtcatca                  <  2:4036241/150‑1 (MQ=255)
                                                                                                                                       accCAGAGCGTTATCACCAGCAACACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCAcc                <  2:2118645/150‑1 (MQ=255)
                                                                                                                                       accCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCAcc                <  1:5189543/150‑1 (MQ=255)
                                                                                                                                       accCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCAcc                >  2:2856334/1‑150 (MQ=255)
                                                                                                                                       accCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCAcc                <  1:2601839/150‑1 (MQ=255)
                                                                                                                                        cccAGAGCGTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCa               >  2:689317/1‑150 (MQ=255)
                                                                                                                                         ccAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCat              <  2:3630376/150‑1 (MQ=255)
                                                                                                                                            gagCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCAtata           >  2:3165205/1‑150 (MQ=255)
                                                                                                                                                gTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTg       <  2:4237709/150‑1 (MQ=255)
                                                                                                                                                gTTATAACCAGCAAAACGGCGACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTg       <  1:1807783/150‑1 (MQ=255)
                                                                                                                                                gTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTg       >  2:1359364/1‑150 (MQ=255)
                                                                                                                                                gTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTg       >  2:1359290/1‑150 (MQ=255)
                                                                                                                                                gTTATAACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTg       <  1:4415840/150‑1 (MQ=255)
                                                                                                                                                   aTCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGaaa    <  2:2593546/150‑1 (MQ=255)
                                                                                                                                                    tCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGaaaa   >  1:2112530/1‑150 (MQ=255)
                                                                                                                                                     cACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAg  <  1:3296848/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAG  >  CP009273/2078372‑2078670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A