Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 481,667 G→A 87.1% A151T (GCG→ACG)  acrR → transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273481,6670GA87.1% 512.3 / 56.7 178A151T (GCG→ACG) acrRtranscriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (6/17);  new base A (81/74);  total (87/91)
Fisher's exact test for biased strand distribution p-value = 2.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

ACAGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTG  >  CP009273/481520‑481813
                                                                                                                                                   |                                                                                                                                                  
aCAGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACGGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACg                                                                                                                                                  >  2:3095433/1‑150 (MQ=255)
    aagaaCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGAACGTATAGAACAAACGTTAAAACATTGTATTGAAACGaaaa                                                                                                                                              >  2:2321297/1‑150 (MQ=255)
      gaaCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATg                                                                                                                                            <  2:762395/150‑1 (MQ=255)
       aaCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAAAGAAAATGt                                                                                                                                           >  2:3253568/1‑150 (MQ=255)
       aaCGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAAATTGTCGGAGAAAAGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATATAACAAACGTTAAAACATTGTATTGAAACGAAAAAGt                                                                                                                                           >  1:596742/1‑150 (MQ=255)
          ggCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGc                                                                                                                                        >  2:2667158/1‑150 (MQ=255)
            tgTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCt                                                                                                                                      >  2:1055412/2‑150 (MQ=255)
            cGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCt                                                                                                                                      <  1:3870924/150‑1 (MQ=255)
             gTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTg                                                                                                                                     >  2:1911083/1‑150 (MQ=255)
               cGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCg                                                                                                                                   <  1:4511440/150‑1 (MQ=255)
                gATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCgg                                                                                                                                  >  2:129076/1‑150 (MQ=255)
                 attattGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGa                                                                                                                                 >  2:3750345/1‑150 (MQ=255)
                 attattGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGa                                                                                                                                 >  2:1355688/1‑150 (MQ=255)
                  ttattGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGAt                                                                                                                                <  1:1077735/150‑1 (MQ=255)
                  ttattGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGAt                                                                                                                                <  1:1077251/150‑1 (MQ=255)
                     ttGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTa                                                                                                                             >  2:1437318/1‑150 (MQ=255)
                     ttGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTa                                                                                                                             <  1:3247230/150‑1 (MQ=255)
                       gATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAAt                                                                                                                           <  2:2730102/150‑1 (MQ=255)
                       gATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAAt                                                                                                                           <  2:3663774/150‑1 (MQ=255)
                           gagaTTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGAcg                                                                                                                       <  1:4245083/150‑1 (MQ=255)
                             gaTTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGAcgcg                                                                                                                     <  1:14032/150‑1 (MQ=255)
                             gaTTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGAcgcg                                                                                                                     <  1:3309107/150‑1 (MQ=255)
                                tataTTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTcg                                                                                                                  >  1:3113629/1‑150 (MQ=255)
                                  tatTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTcgcg                                                                                                                >  1:3730186/1‑150 (MQ=255)
                                  tatTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACAGTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTcgcg                                                                                                                >  2:1169260/1‑150 (MQ=255)
                                  tatTACACAAATGCGAATTTGTCTGAGAAATGGATGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATAGTATTGAAACGAAAATGTTTCCTGCTGATTTAATTACGCGTcgcg                                                                                                                >  2:2281046/1‑150 (MQ=255)
                                   atTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCgcgc                                                                                                               <  2:1802745/150‑1 (MQ=255)
                                    ttCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCgca                                                                                                              >  1:1898717/1‑150 (MQ=255)
                                      ccACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCgcagc                                                                                                            >  1:1976243/1‑150 (MQ=255)
                                       cacaAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCgcagca                                                                                                           >  1:2239903/1‑150 (MQ=255)
                                       cacaAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCgcagca                                                                                                           <  2:3474555/150‑1 (MQ=255)
                                         caAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAat                                                                                                         <  2:4196110/150‑1 (MQ=255)
                                          aaaTGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAatt                                                                                                        <  2:4291903/150‑1 (MQ=255)
                                           aaTGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGTAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAatta                                                                                                       >  1:1592635/1‑150 (MQ=255)
                                            aTGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAattat                                                                                                      >  2:3701797/1‑150 (MQ=255)
                                            aTGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAattat                                                                                                      <  1:1168581/150‑1 (MQ=255)
                                            aTGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAattat                                                                                                      <  2:3332449/150‑1 (MQ=255)
                                            aTGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAattat                                                                                                      <  2:3332729/150‑1 (MQ=255)
                                              gCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAAttatta                                                                                                    <  2:2654551/150‑1 (MQ=255)
                                                     tGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCgg                                                                                             >  2:2448176/1‑150 (MQ=255)
                                                       tCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACTGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCt                                                                                           <  2:213676/150‑1 (MQ=255)
                                                         ggAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCtat                                                                                         <  1:1900250/150‑1 (MQ=255)
                                                         ggAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCtat                                                                                         <  1:1900255/150‑1 (MQ=255)
                                                          gagaAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCtata                                                                                        <  1:1437318/150‑1 (MQ=255)
                                                          gagaAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCtata                                                                                        <  2:2589937/150‑1 (MQ=255)
                                                             aaaTGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATAttt                                                                                     <  2:320722/150‑1 (MQ=255)
                                                             aaaTGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATAttt                                                                                     <  2:603342/150‑1 (MQ=255)
                                                              aaTGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTc                                                                                    >  2:1177814/1‑150 (MQ=255)
                                                               aTGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTcc                                                                                   <  2:2737558/150‑1 (MQ=255)
                                                                        gtgCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATg                                                                          <  2:3994527/150‑1 (MQ=255)
                                                                        gtgCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATg                                                                          <  2:1607503/150‑1 (MQ=255)
                                                                           cAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGaa                                                                       <  2:4469389/150‑1 (MQ=255)
                                                                             aCAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGaaaa                                                                     >  1:3935257/1‑150 (MQ=255)
                                                                              cAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAAc                                                                    >  2:3103751/1‑150 (MQ=255)
                                                                               aGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACt                                                                   <  2:1856571/150‑1 (MQ=255)
                                                                                  cacaACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGc                                                                <  2:579286/150‑1 (MQ=255)
                                                                                   acaACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGct                                                               <  2:818452/150‑1 (MQ=255)
                                                                                    caACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGctc                                                              >  1:4712074/1‑150 (MQ=255)
                                                                                    caACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGctc                                                              >  1:4710763/1‑150 (MQ=255)
                                                                                     aaCGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGctct                                                             <  1:1860591/150‑1 (MQ=255)
                                                                                     aaCGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGctct                                                             >  2:459577/1‑150 (MQ=255)
                                                                                      aCGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCtt                                                            <  1:1721231/150‑1 (MQ=255)
                                                                                       cGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCttt                                                           <  1:2667158/150‑1 (MQ=255)
                                                                                       cGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCttt                                                           <  1:2940094/150‑1 (MQ=255)
                                                                                       cGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCttt                                                           >  1:1594368/1‑150 (MQ=255)
                                                                                        gTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTg                                                          >  1:365221/1‑150 (MQ=255)
                                                                                               ctgtctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCa                                                   >  2:1424163/1‑150 (MQ=255)
                                                                                                   ctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATTCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCACCGCAATCt                                               >  1:94320/1‑150 (MQ=255)
                                                                                                   ctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCt                                               <  1:4138975/150‑1 (MQ=255)
                                                                                                   ctgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCt                                               >  1:4250002/1‑150 (MQ=255)
                                                                                                    tgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCtt                                              >  1:1743408/1‑150 (MQ=255)
                                                                                                    tgGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCtt                                              <  1:2306515/150‑1 (MQ=255)
                                                                                                     ggAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCttt                                             <  1:3557293/150‑1 (MQ=255)
                                                                                                     ggAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCttt                                             >  2:4036174/1‑150 (MQ=255)
                                                                                                      gAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCtttt                                            <  2:3164960/150‑1 (MQ=255)
                                                                                                                  cGTATAGAACAAACGTTAAAACATTGTATTGAAACGAATATGGTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGATATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTaaaaaa                                <  1:2276176/150‑1 (MQ=255)
                                                                                                                    tataGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaaga                              >  1:2661732/1‑150 (MQ=255)
                                                                                                                    tataGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaaga                              <  2:2581171/150‑1 (MQ=255)
                                                                                                                    tataGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaaga                              <  1:705805/150‑1 (MQ=255)
                                                                                                                    tataGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaaga                              >  1:2538110/1‑150 (MQ=255)
                                                                                                                     ataGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaagaa                             <  1:637430/150‑1 (MQ=255)
                                                                                                                     ataGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaagaa                             >  1:744196/1‑150 (MQ=255)
                                                                                                                         aaCAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGccc                         <  2:15402/150‑1 (MQ=255)
                                                                                                                          aCAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCcg                        >  2:4450151/1‑150 (MQ=255)
                                                                                                                          aCAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCcg                        >  2:588080/1‑150 (MQ=255)
                                                                                                                          aCAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCcg                        >  2:4449904/1‑150 (MQ=255)
                                                                                                                          aCAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGAAGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAATGAAGCCcg                        >  2:4450022/1‑150 (MQ=255)
                                                                                                                           cAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCcgc                       >  1:4660189/1‑150 (MQ=255)
                                                                                                                            aaaCGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCcgcg                      >  1:822752/1‑150 (MQ=255)
                                                                                                                            aaaCGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCcgcg                      >  1:3188530/1‑150 (MQ=255)
                                                                                                                              ccGTTAAACCATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGAt                    <  1:4250140/149‑1 (MQ=255)
                                                                                                                               cGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGAtt                   >  1:1423692/1‑150 (MQ=255)
                                                                                                                               cGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGAtt                   >  2:3645070/1‑150 (MQ=255)
                                                                                                                                 ttAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCAGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTAc                 >  1:24608/1‑150 (MQ=255)
                                                                                                                                  tAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACg                >  2:172404/1‑150 (MQ=255)
                                                                                                                                   aaaaCCTTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTAAGt               <  2:4154821/150‑1 (MQ=255)
                                                                                                                                      aCATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGc            >  1:4309573/1‑150 (MQ=255)
                                                                                                                                       cATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGcc           <  1:1473293/150‑1 (MQ=255)
                                                                                                                                            tATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCtt      >  1:3418978/1‑150 (MQ=255)
                                                                                                                                                gAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTg  >  2:633772/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACAGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTG  >  CP009273/481520‑481813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGACAGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGG  >  CP009273/481518‑481814
                                                                                                                                                     |                                                                                                                                                   
TGACAGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAA                                                                                                                                                     <  SRR19973082.1559552/150‑1 (MQ=60)
         AACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAAATTGTCGGAGAAAAGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATATAACAAACGTTAAAACATTGTATTGAAACGAAAAAGT                                                                                                                                            >  SRR19973082.600985/1‑150 (MQ=60)
              CGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCT                                                                                                                                       <  SRR19973082.3894658/150‑1 (MQ=60)
                 CGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCG                                                                                                                                    >  SRR19973082.4655125/1‑150 (MQ=60)
                 CGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCG                                                                                                                                    <  SRR19973082.4538878/150‑1 (MQ=60)
                    TTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGAT                                                                                                                                 <  SRR19973082.1085090/150‑1 (MQ=60)
                    TTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGAT                                                                                                                                 <  SRR19973082.1084602/150‑1 (MQ=60)
                       TTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTA                                                                                                                              <  SRR19973082.3267422/150‑1 (MQ=60)
                       TTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTA                                                                                                                              <  SRR19973082.3267170/150‑1 (MQ=60)
                         GATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAAT                                                                                                                            <  SRR19973082.2277570/150‑1 (MQ=60)
                             GAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACG                                                                                                                        <  SRR19973082.4270949/150‑1 (MQ=60)
                             GAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACG                                                                                                                        <  SRR19973082.4270916/150‑1 (MQ=60)
                             GAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACG                                                                                                                        <  SRR19973082.1876794/150‑1 (MQ=60)
                               GATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCG                                                                                                                      <  SRR19973082.14240/150‑1 (MQ=60)
                               GATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCG                                                                                                                      <  SRR19973082.3329561/150‑1 (MQ=60)
                                  TATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCG                                                                                                                   >  SRR19973082.3132557/1‑150 (MQ=60)
                                   ATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGC                                                                                                                  >  SRR19973082.3647690/1‑150 (MQ=60)
                                   ATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGC                                                                                                                  >  SRR19973082.217349/1‑150 (MQ=60)
                                   AGATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTCATGACGCGTCGC                                                                                                                  >  SRR19973082.1940687/1‑150 (MQ=60)
                                    TATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCG                                                                                                                 >  SRR19973082.3753270/1‑150 (MQ=60)
                                      TTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCA                                                                                                               >  SRR19973082.1910885/1‑150 (MQ=60)
                                      TTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCA                                                                                                               >  SRR19973082.1911319/1‑150 (MQ=60)
                                        CCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGC                                                                                                             >  SRR19973082.1988605/1‑150 (MQ=60)
                                         CACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCA                                                                                                            >  SRR19973082.2254004/1‑150 (MQ=60)
                                           CAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAAT                                                                                                          <  SRR19973082.4104723/150‑1 (MQ=60)
                                            AAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATT                                                                                                         >  SRR19973082.4180254/1‑150 (MQ=60)
                                             AATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGTAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTA                                                                                                        >  SRR19973082.1603098/1‑150 (MQ=60)
                                             AATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGTAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTA                                                                                                        >  SRR19973082.1603164/1‑150 (MQ=60)
                                              ATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTAT                                                                                                       <  SRR19973082.1729306/150‑1 (MQ=60)
                                              ATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTAT                                                                                                       >  SRR19973082.141109/1‑150 (MQ=60)
                                              ATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTAT                                                                                                       <  SRR19973082.1176234/150‑1 (MQ=60)
                                                  GAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATG                                                                                                   <  SRR19973082.2301684/150‑1 (MQ=60)
                                                    ATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCG                                                                                                 >  SRR19973082.1454113/1‑150 (MQ=60)
                                                    ATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCG                                                                                                 >  SRR19973082.3712909/1‑150 (MQ=60)
                                                          CGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACGTTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTA                                                                                           <  SRR19973082.84061/150‑1 (MQ=60)
                                                          CGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTA                                                                                           <  SRR19973082.2549330/150‑1 (MQ=60)
                                                           GGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTAT                                                                                          <  SRR19973082.1912428/150‑1 (MQ=60)
                                                           GGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTAT                                                                                          >  SRR19973082.393709/1‑150 (MQ=60)
                                                           GGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTAT                                                                                          <  SRR19973082.1912423/150‑1 (MQ=60)
                                                            GAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA                                                                                         <  SRR19973082.1447091/150‑1 (MQ=60)
                                                                    GCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGC                                                                                 >  SRR19973082.4320966/1‑150 (MQ=60)
                                                                           TGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGG                                                                          <  SRR19973082.3558519/150‑1 (MQ=60)
                                                                               ACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAA                                                                      >  SRR19973082.3959206/1‑150 (MQ=60)
                                                                                      CAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTC                                                               >  SRR19973082.4740445/1‑150 (MQ=60)
                                                                                      CAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTC                                                               >  SRR19973082.4739130/1‑150 (MQ=60)
                                                                                       AACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCT                                                              <  SRR19973082.1872644/150‑1 (MQ=60)
                                                                                        ACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTT                                                             <  SRR19973082.1732669/150‑1 (MQ=60)
                                                                                         CGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTT                                                            <  SRR19973082.790558/150‑1 (MQ=60)
                                                                                         CGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTT                                                            >  SRR19973082.1604853/1‑150 (MQ=60)
                                                                                         CGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTT                                                            <  SRR19973082.2958265/150‑1 (MQ=60)
                                                                                         CGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTT                                                            <  SRR19973082.2683573/150‑1 (MQ=60)
                                                                                          GTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTG                                                           >  SRR19973082.367583/1‑150 (MQ=60)
                                                                                          GTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTG                                                           <  SRR19973082.1421507/150‑1 (MQ=60)
                                                                                                   GTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAAT                                                  <  SRR19973082.3149177/150‑1 (MQ=60)
                                                                                                     CTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATTCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCACCGCAATCT                                                >  SRR19973082.95270/1‑150 (MQ=60)
                                                                                                     CTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCT                                                >  SRR19973082.4275848/1‑150 (MQ=60)
                                                                                                     CTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCT                                                <  SRR19973082.4164334/150‑1 (MQ=60)
                                                                                                      TGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTT                                               <  SRR19973082.2320852/150‑1 (MQ=60)
                                                                                                      TGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTT                                               >  SRR19973082.1754975/1‑150 (MQ=60)
                                                                                                      TGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTT                                               >  SRR19973082.1755736/1‑150 (MQ=60)
                                                                                                      TGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTT                                               <  SRR19973082.2670204/150‑1 (MQ=60)
                                                                                                       GGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTT                                              <  SRR19973082.3578868/150‑1 (MQ=60)
                                                                                                          AAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGA                                           <  SRR19973082.2973538/150‑1 (MQ=60)
                                                                                                                  ACCGTATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAA                                   <  SRR19973082.4456864/150‑1 (MQ=60)
                                                                                                                    CGTATAGAACAAACGTTAAAACATTGTATTGAAACGAATATGGTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGATATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAA                                 <  SRR19973082.2290408/150‑1 (MQ=60)
                                                                                                                      TATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGA                               >  SRR19973082.2553951/1‑150 (MQ=60)
                                                                                                                      TATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGA                               <  SRR19973082.710559/150‑1 (MQ=60)
                                                                                                                      TATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGA                               >  SRR19973082.4740150/1‑150 (MQ=60)
                                                                                                                      TATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGA                               >  SRR19973082.2678289/1‑150 (MQ=60)
                                                                                                                      TATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGA                               >  SRR19973082.2678127/1‑150 (MQ=60)
                                                                                                                       ATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTGTATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAA                              >  SRR19973082.4775857/1‑150 (MQ=60)
                                                                                                                       ATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAA                              >  SRR19973082.749081/1‑150 (MQ=60)
                                                                                                                       ATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAA                              <  SRR19973082.641870/150‑1 (MQ=60)
                                                                                                                       ATAGAACAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAA                              <  SRR19973082.641894/150‑1 (MQ=60)
                                                                                                                             CAAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGC                        >  SRR19973082.4688363/1‑150 (MQ=60)
                                                                                                                              AAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCG                       >  SRR19973082.828139/1‑150 (MQ=60)
                                                                                                                              AAACGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCG                       >  SRR19973082.3207862/1‑150 (MQ=60)
                                                                                                                                CCGTTAAACCATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGAT                     <  SRR19973082.4275986/150‑1 (MQ=60)
                                                                                                                                 CGTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATT                    >  SRR19973082.1433408/1‑150 (MQ=60)
                                                                                                                                  GTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTA                   >  SRR19973082.3240906/1‑150 (MQ=60)
                                                                                                                                  GTTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTA                   <  SRR19973082.1090136/150‑1 (MQ=60)
                                                                                                                                   TTAAAACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCAGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTAC                  >  SRR19973082.24939/1‑150 (MQ=60)
                                                                                                                                        ACATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGC             >  SRR19973082.4335616/1‑150 (MQ=60)
                                                                                                                                         CATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCC            <  SRR19973082.1483210/150‑1 (MQ=60)
                                                                                                                                          ATTGTATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCA           >  SRR19973082.1570153/1‑150 (MQ=60)
                                                                                                                                              TATTGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTT       >  SRR19973082.3440066/1‑150 (MQ=60)
                                                                                                                                                 TGAAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACT    <  SRR19973082.637149/150‑1 (MQ=60)
                                                                                                                                                   AAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGG  >  SRR19973082.556921/1‑150 (MQ=60)
                                                                                                                                                   AAACGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGG  >  SRR19973082.2021943/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
TGACAGAAGAACGGCGTCGATTATTGATGGAGATTATATTCCACAAATGCGAATTTGTCGGAGAAATGGCTGTTGTGCAACAGGCACAACGTAATCTCTGTCTGGAAAGTTATGACCGTATAGAACAAACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTACTGG  >  CP009273/481518‑481814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑