Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 631,333 G→A 5.1% N231N (AAC→AAT)  ybdN ← PAPS reductase‑like domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273631,3330GA 5.1% 1055.0 / 28.3 294N231N (AAC→AAT) ybdNPAPS reductase‑like domain protein
Reads supporting (aligned to +/- strand):  ref base G (136/142);  new base A (8/7);  total (144/150)
Fisher's exact test for biased strand distribution p-value = 7.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01

Only 100 of 299 total aligned reads displayed.

CCACTTTTTACCCCGCTGACTCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACGTTGTTTATT  >  CP009273/631184‑631482
                                                                                                                                                     |                                                                                                                                                     
  aCTTTTTACCCCGCTGACTCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGtt                                                                                                                                                     <  1:1487303/150‑1 (MQ=255)
  aCTTTTTACCCCGCTGACTCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGtt                                                                                                                                                     <  2:4735025/150‑1 (MQ=255)
   cTTTTTACCCCGCTGACTCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTta                                                                                                                                                    >  2:404121/1‑150 (MQ=255)
    tttttACCCCGCTGACTCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTtat                                                                                                                                                   <  2:2710776/150‑1 (MQ=255)
       ttACCCCGCTGACTCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACa                                                                                                                                                <  2:2211879/150‑1 (MQ=255)
         aCCCCGCTGACTCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGt                                                                                                                                              <  1:607177/150‑1 (MQ=255)
             cGCTGACTCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGt                                                                                                                                          <  1:2602731/150‑1 (MQ=255)
              gCTGACTCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGtt                                                                                                                                         <  1:1189712/150‑1 (MQ=255)
               cTGACTCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTg                                                                                                                                        <  1:2777775/150‑1 (MQ=255)
                tGACTCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGc                                                                                                                                       >  2:4674090/1‑150 (MQ=255)
                tGACTCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGc                                                                                                                                       >  2:2620280/1‑150 (MQ=255)
                tGACTCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGc                                                                                                                                       >  1:1103089/1‑150 (MQ=255)
                 gACTCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCa                                                                                                                                      >  2:4706320/1‑150 (MQ=255)
                 gACTCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCa                                                                                                                                      >  1:4295058/1‑150 (MQ=255)
                 gACTCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGAGAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCa                                                                                                                                      >  1:1859318/1‑150 (MQ=255)
                  aCTCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCag                                                                                                                                     >  1:2249790/1‑150 (MQ=255)
                    tCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCagag                                                                                                                                   >  2:3162383/1‑150 (MQ=255)
                     cGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAgaga                                                                                                                                  >  2:3806551/1‑150 (MQ=255)
                      gTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGAc                                                                                                                                 >  2:2597041/1‑150 (MQ=255)
                        gcgcACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACtt                                                                                                                               <  1:3716285/150‑1 (MQ=255)
                         cgcACATTGCTGCGCAGAGATCAGGTTCGAGAACGTGATAGAGCCATTATCATTGTCGTTGCTCCGTGACAAAAGGTTCTCAAATTCGAATTTGACGCAGAGGAACGCCTGACTGATACATTAGGTTATACAGTGGTTTGCAGAGACttt                                                                                                                              <  2:741494/150‑1 (MQ=255)
                         cgcACATCGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACttt                                                                                                                              <  2:4289612/150‑1 (MQ=255)
                         cgcACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACttt                                                                                                                              <  1:3510925/150‑1 (MQ=255)
                          gcACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTtg                                                                                                                             <  2:130558/150‑1 (MQ=255)
                          gcACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTtg                                                                                                                             >  1:301856/1‑150 (MQ=255)
                            acaTAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCTGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTtgat                                                                                                                           >  2:3381945/1‑150 (MQ=255)
                            acaTAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTtgat                                                                                                                           <  1:1297826/150‑1 (MQ=255)
                            acaTAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTtgat                                                                                                                           <  2:4216595/150‑1 (MQ=255)
                                 gcagcCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGAttt                                                                                                                      <  2:4255574/150‑1 (MQ=255)
                                  cagcCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTg                                                                                                                     >  2:1782523/1‑150 (MQ=255)
                                  cagcCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTg                                                                                                                     >  1:670564/1‑150 (MQ=255)
                                  cagcCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTg                                                                                                                     >  1:525636/1‑150 (MQ=255)
                                      ccAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATa                                                                                                                 <  2:3890579/150‑1 (MQ=255)
                                       cAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATAc                                                                                                                <  2:1741365/150‑1 (MQ=255)
                                          cGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAg                                                                                                             >  2:1450484/1‑150 (MQ=255)
                                            aTCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGt                                                                                                           <  2:1168481/150‑1 (MQ=255)
                                             tCCGGTTCGATAACGTGATAGAGCCAGAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTc                                                                                                          <  2:1717155/150‑1 (MQ=255)
                                                ggTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAg                                                                                                       >  2:4267135/1‑150 (MQ=255)
                                                 gTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGa                                                                                                      <  1:4488192/150‑1 (MQ=255)
                                                 gTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGa                                                                                                      <  1:892361/150‑1 (MQ=255)
                                                        aaCGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGc                                                                                               >  2:1262352/1‑150 (MQ=255)
                                                           gTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCAc                                                                                            >  2:1097059/1‑150 (MQ=255)
                                                           gTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCAc                                                                                            <  1:3892330/150‑1 (MQ=255)
                                                           gTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGATTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCAc                                                                                            >  2:1474661/1‑150 (MQ=255)
                                                            tGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACt                                                                                           >  2:4509410/1‑150 (MQ=255)
                                                            tGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACt                                                                                           <  1:2333825/150‑1 (MQ=255)
                                                            tGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACt                                                                                           >  1:1657113/1‑150 (MQ=255)
                                                             gATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGCTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACtt                                                                                          <  1:1954915/150‑1 (MQ=255)
                                                              aTAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACttt                                                                                         <  2:1904538/150‑1 (MQ=255)
                                                                  agCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAg                                                                                     >  1:3285570/1‑150 (MQ=255)
                                                                   gCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGt                                                                                    <  1:4107872/150‑1 (MQ=255)
                                                                    ccATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGATTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTc                                                                                   >  2:2181074/1‑150 (MQ=255)
                                                                           ccTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATg                                                                            <  2:2424566/150‑1 (MQ=255)
                                                                            cTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATgg                                                                           >  1:89825/1‑150 (MQ=255)
                                                                             ttGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATggg                                                                          <  2:1392360/150‑1 (MQ=255)
                                                                             ttGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATggg                                                                          >  2:1963754/1‑150 (MQ=255)
                                                                                    tGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATg                                                                   >  1:763053/1‑150 (MQ=255)
                                                                                    tGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATg                                                                   <  2:1645262/150‑1 (MQ=255)
                                                                                    tGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATg                                                                   <  1:3991466/150‑1 (MQ=255)
                                                                                      cTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTa                                                                 >  1:2068150/1‑150 (MQ=255)
                                                                                        ccGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTAcc                                                               <  1:2759081/150‑1 (MQ=255)
                                                                                         cGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCa                                                              >  1:1610904/1‑150 (MQ=255)
                                                                                             ccAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTa                                                          >  2:2276103/1‑150 (MQ=255)
                                                                                              cAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGATTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCAAGTCGTAAATGGGGTAAATGTACCAGCTAt                                                         >  2:1145592/1‑150 (MQ=255)
                                                                                               aaaaGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATg                                                        <  2:3734322/150‑1 (MQ=255)
                                                                                                aaaGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGa                                                       <  1:4748566/150‑1 (MQ=255)
                                                                                                  aGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGAcc                                                     >  2:3569340/1‑150 (MQ=255)
                                                                                                    gTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGc                                                   >  2:3966648/1‑150 (MQ=255)
                                                                                                      tCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCt                                                 <  2:3324182/150‑1 (MQ=255)
                                                                                                      tCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCt                                                 <  1:4323714/150‑1 (MQ=255)
                                                                                                       cGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTg                                                <  2:90066/150‑1 (MQ=255)
                                                                                                       cGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTg                                                >  2:934033/1‑150 (MQ=255)
                                                                                                       cGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTg                                                >  1:2939712/1‑150 (MQ=255)
                                                                                                        gCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTgg                                               <  2:3082332/150‑1 (MQ=255)
                                                                                                            aTTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTTCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCg                                           >  2:3742964/1‑150 (MQ=255)
                                                                                                            aTTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCg                                           >  2:2248125/1‑150 (MQ=255)
                                                                                                               cGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGATTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGcc                                        <  2:2097002/150‑1 (MQ=255)
                                                                                                                    atGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGt                                   <  2:2696218/150‑1 (MQ=255)
                                                                                                                       aCGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCa                                >  2:100267/1‑150 (MQ=255)
                                                                                                                          cAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAggg                             <  1:2620280/150‑1 (MQ=255)
                                                                                                                          cAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAggg                             <  1:244855/150‑1 (MQ=255)
                                                                                                                           agagGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGt                            >  1:4275334/1‑150 (MQ=255)
                                                                                                                             agGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATTATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGttt                          <  2:3512422/150‑1 (MQ=255)
                                                                                                                               gAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTAt                        <  2:1417778/150‑1 (MQ=255)
                                                                                                                                ttcGCCTGCCTGTTACATTAGGTTTTACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGTTTTCAGCCACTTTCCAGTCGTTAATGGGGTATATGTACCATCTATGTCCGCCTGGTGCGGCCGTAGTCCAGGGTTTAtc                       <  1:3912045/148‑1 (MQ=255)
                                                                                                                                   gcctgcctGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTAtcgtc                    >  2:1361620/1‑150 (MQ=255)
                                                                                                                                    cctgcctgATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTAtcgtcg                   <  1:3364065/150‑1 (MQ=255)
                                                                                                                                         ctgATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCaaa              <  1:3608235/150‑1 (MQ=255)
                                                                                                                                          tgATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCaaaa             >  1:3087961/1‑150 (MQ=255)
                                                                                                                                           gATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAAc            <  1:81919/150‑1 (MQ=255)
                                                                                                                                           gATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAAc            >  1:617622/1‑150 (MQ=255)
                                                                                                                                             tACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACgt          <  1:4502457/150‑1 (MQ=255)
                                                                                                                                              aCATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACgtt         >  1:4185168/1‑150 (MQ=255)
                                                                                                                                                 ttAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACgttgtt      <  1:4674090/150‑1 (MQ=255)
                                                                                                                                                 ttAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACgttgtt      <  2:672289/150‑1 (MQ=255)
                                                                                                                                                 ttAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACgttgtt      <  2:2858019/150‑1 (MQ=255)
                                                                                                                                                  tAGGTTAGACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACGTTGttt     <  2:3414670/150‑1 (MQ=255)
                                                                                                                                                   aGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCACAACGTTGttta    >  2:2514922/1‑150 (MQ=255)
                                                                                                                                                   aGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACGTTGttta    >  1:293738/1‑150 (MQ=255)
                                                                                                                                                     gTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACGTTGtttatt  <  2:3473095/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCACTTTTTACCCCGCTGACTCGTGCGCACATAGCAGCCCAGCGATCCGGTTCGATAACGTGATAGAGCCATAATCCTTGTCGTTGCTCCGGGCCAAAAGGTTCGCAAATTCGCATATGACGCAGAGGAACGCCTGCCTGATACATTAGGTTATACAGTGGGTTGCAGAGACTTTGATGATTTGCATACCAGGTCCAGATATCAGCCACTTTCCAGTCGTAAATGGGGTAAATGTACCAGCTATGACCGCCTGGTGCGGCCGTAGTCCAGGGTTTATCGTCGGCAAAACGTTGTTTATT  >  CP009273/631184‑631482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A