Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,124,159 C→A 12.0% A67A (GCG→GCT)  wcaD ← putative colanic acid polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,124,1590CA12.0% 741.1 / 61.2 217A67A (GCG→GCT) wcaDputative colanic acid polymerase
Reads supporting (aligned to +/- strand):  ref base C (125/66);  new base A (16/10);  total (141/76)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 222 total aligned reads displayed.

AAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAG  >  CP009273/2124010‑2124308
                                                                                                                                                     |                                                                                                                                                      
aaaCGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAAc                                                                                                                                                        <  1:2659520/150‑1 (MQ=255)
aaaCGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAAc                                                                                                                                                        <  1:963225/150‑1 (MQ=255)
aaaCGTAATATCTTGCGATGGTTGTGTGGCGAAATTGATTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAAc                                                                                                                                                        <  1:3229075/150‑1 (MQ=255)
  aCGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGc                                                                                                                                                      >  2:4516594/1‑150 (MQ=255)
    ttAATATATTGCGATGGTTGTGTGGCTAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCg                                                                                                                                                    <  1:630311/149‑1 (MQ=255)
    gTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCg                                                                                                                                                    >  2:447695/1‑150 (MQ=255)
     tAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGt                                                                                                                                                   >  2:4351181/1‑150 (MQ=255)
     tAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGt                                                                                                                                                   >  2:2154421/1‑150 (MQ=255)
        tatCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAAAGCCGTGAg                                                                                                                                                >  2:1211628/1‑150 (MQ=255)
           cTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTcc                                                                                                                                             >  1:1902683/1‑150 (MQ=255)
                 aTGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGcc                                                                                                                                       >  2:4325807/1‑150 (MQ=255)
                    gTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAAt                                                                                                                                    >  1:1479498/1‑150 (MQ=255)
                      tgtgtgGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATcc                                                                                                                                  >  2:1934414/1‑150 (MQ=255)
                        tgtgGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAGTATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCt                                                                                                                                <  2:4288587/150‑1 (MQ=255)
                        tgtgGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCt                                                                                                                                >  1:2060539/1‑150 (MQ=255)
                        tgtgGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCt                                                                                                                                <  2:1830099/150‑1 (MQ=255)
                          tgGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAAAGCCGTGAGTCCCGCGCCAATCCCTaa                                                                                                                              <  1:3261464/150‑1 (MQ=255)
                          tgGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAAAGCCGTGAGTCCCGCGCCAATCCCTaa                                                                                                                              <  1:3382274/150‑1 (MQ=255)
                           ggCGAAATTTTTTTAAAACGAATACTCCACACCATGCAAATAATGATCAAAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAg                                                                                                                             <  2:4510912/150‑1 (MQ=255)
                           ggCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAg                                                                                                                             <  2:649407/150‑1 (MQ=255)
                              gAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCa                                                                                                                          <  2:160035/150‑1 (MQ=255)
                               aaaTTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCaa                                                                                                                         <  2:2763425/150‑1 (MQ=255)
                               aaaTTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCaa                                                                                                                         >  1:57451/1‑150 (MQ=255)
                               aaaTTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCaa                                                                                                                         >  2:3943929/1‑150 (MQ=255)
                                 aTTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAAAGCCGTGAGTCCCGCGCCAATCCCTAAGGCAatc                                                                                                                       >  2:4558998/1‑150 (MQ=255)
                                   tGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAatcat                                                                                                                     <  1:961427/150‑1 (MQ=255)
                                    gTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAatcatc                                                                                                                    >  1:3830277/1‑150 (MQ=255)
                                     ttttAAAACGAATACTCCACACCATGCAAATAATGATCACAATATAGACAAACAGCATAGTTGATGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAAtcatca                                                                                                                   <  1:89124/150‑1 (MQ=255)
                                       ttAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAAAGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑t                                                                                                                <  1:357737/150‑1 (MQ=255)
                                       ttAAAACGAATACGCCACACCATGACAATAATTATCAAAATATACACAAACAGATTTTTTGATGTGACTTTTTTACGGGCATCCAGCGACTGACCAAACAGATTTTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑t                                                                                                                <  1:12693/150‑1 (MQ=255)
                                        tAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑tt                                                                                                               >  1:915666/1‑150 (MQ=255)
                                         aaaaCGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑ttt                                                                                                              >  1:3946184/1‑150 (MQ=255)
                                          aaaCGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑tttt                                                                                                             >  2:3873068/1‑150 (MQ=255)
                                            aCGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑tttttt                                                                                                           >  1:1070470/1‑150 (MQ=255)
                                              gAATACTCCACACCATGCCAATAATGATCACAATATAGACAACCAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTaa                                                                                                         <  1:1152863/150‑1 (MQ=255)
                                               aaTACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATATTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAAc                                                                                                        <  2:1080706/150‑1 (MQ=255)
                                                aTACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACg                                                                                                       >  1:699680/1‑150 (MQ=255)
                                                aTACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACg                                                                                                       >  1:2853206/1‑150 (MQ=255)
                                                    tCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGa                                                                                                   >  1:3543127/1‑150 (MQ=255)
                                                    tCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGa                                                                                                   >  2:472947/1‑150 (MQ=255)
                                                          ccATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGtt                                                                                             >  2:281438/1‑150 (MQ=255)
                                                            aTGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCt                                                                                           >  2:2526339/1‑150 (MQ=255)
                                                            aTGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGGGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCt                                                                                           <  1:4408408/150‑1 (MQ=255)
                                                              gcaaataatGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTaa                                                                                         <  2:3910234/147‑1 (MQ=255)
                                                              gCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTaa                                                                                         <  1:1001666/150‑1 (MQ=255)
                                                                 aataatGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTaaaaa                                                                                      >  1:4296706/1‑150 (MQ=255)
                                                                   taatGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAAca                                                                                    >  1:4705076/1‑150 (MQ=255)
                                                                       gATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAAcagcag                                                                                >  2:2438256/1‑150 (MQ=255)
                                                                        aTCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGt                                                                               >  2:1352715/1‑150 (MQ=255)
                                                                         tCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTa                                                                              >  1:2006211/1‑150 (MQ=255)
                                                                         tCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTa                                                                              >  1:686487/1‑150 (MQ=255)
                                                                          cacaATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTaa                                                                             <  2:4364114/150‑1 (MQ=255)
                                                                          cacaATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAAAGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTaa                                                                             >  2:5083/1‑150 (MQ=255)
                                                                             aaTATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACaa                                                                          <  2:4551318/150‑1 (MQ=255)
                                                                                ataGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGAc                                                                       >  1:2049232/1‑150 (MQ=255)
                                                                                 taGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACa                                                                      >  2:2299286/1‑150 (MQ=255)
                                                                                 taGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACa                                                                      >  2:2299078/1‑150 (MQ=255)
                                                                                   gACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAgg                                                                    >  1:2214620/1‑150 (MQ=255)
                                                                                      aaaCAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTaa                                                                 >  1:637128/1‑150 (MQ=255)
                                                                                        aCAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAAAGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTaaaa                                                               >  1:1772430/1‑150 (MQ=255)
                                                                                        aCAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAAAGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTaaaa                                                               >  1:590471/1‑150 (MQ=255)
                                                                                         cAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAAAGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTaaaaa                                                              >  1:2184516/1‑150 (MQ=255)
                                                                                           gCATATTTGAAGTGACTTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAg                                                            <  1:2362990/150‑1 (MQ=255)
                                                                                             aTAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAATCGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTg                                                          <  2:3694072/150‑1 (MQ=255)
                                                                                                    aaGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATgg                                                   >  1:3346149/1‑150 (MQ=255)
                                                                                                    aaGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAAAGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATgg                                                   >  2:2106641/1‑150 (MQ=255)
                                                                                                     aGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGt                                                  >  1:4527591/1‑150 (MQ=255)
                                                                                                       tGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAGTCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGa                                                >  2:2986086/1‑150 (MQ=255)
                                                                                                           gTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATggg                                            >  1:2498451/1‑150 (MQ=255)
                                                                                                           gTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAAAGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATggg                                            <  1:4033755/150‑1 (MQ=255)
                                                                                                            tATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATgggg                                           >  2:4129357/1‑150 (MQ=255)
                                                                                                            tATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATgggg                                           >  2:3698636/1‑150 (MQ=255)
                                                                                                             aTTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGa                                          >  2:4153909/1‑150 (MQ=255)
                                                                                                              tttACTGGCATCCAGCGACTGACCAAACAGATAGTTAAAAGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGaa                                         >  1:1377546/1‑150 (MQ=255)
                                                                                                                  cTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAATTTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAAca                                      <  1:293454/150‑2 (MQ=255)
                                                                                                                  cTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACtt                                     >  2:1727191/1‑150 (MQ=255)
                                                                                                                  cTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACtt                                     >  2:4220491/1‑150 (MQ=255)
                                                                                                                  cTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACtt                                     >  2:1156385/1‑150 (MQ=255)
                                                                                                                    ggCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTc                                   >  1:1193400/1‑150 (MQ=255)
                                                                                                                           aGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACACGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGc                            >  2:442926/1‑150 (MQ=255)
                                                                                                                                 tGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCg                      <  1:1352715/150‑1 (MQ=255)
                                                                                                                                  gACCAAACAGATAGTTAAAAGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGa                     >  2:2915077/1‑150 (MQ=255)
                                                                                                                                   aCCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGAt                    <  1:4515799/150‑1 (MQ=255)
                                                                                                                                      aaaCAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGAtttt                 >  1:2158912/1‑150 (MQ=255)
                                                                                                                                      aaaCAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGAtttt                 <  1:99875/150‑1 (MQ=255)
                                                                                                                                       aaCAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTg                <  2:3326621/150‑1 (MQ=255)
                                                                                                                                       aaCAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTg                <  1:3873068/150‑1 (MQ=255)
                                                                                                                                         cAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGGAAAAAAGTGACGAGGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGAc              >  2:1621130/1‑150 (MQ=255)
                                                                                                                                            aTAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGtt           <  1:4333426/150‑1 (MQ=255)
                                                                                                                                            aTAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGtt           >  1:4272092/1‑150 (MQ=255)
                                                                                                                                            aTAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGtt           <  2:637128/150‑1 (MQ=255)
                                                                                                                                             tAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTa          <  2:1902683/150‑1 (MQ=255)
                                                                                                                                             tAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTa          >  2:3433268/1‑150 (MQ=255)
                                                                                                                                               gTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAAc        <  1:2718278/150‑1 (MQ=255)
                                                                                                                                                ttAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAAcc       >  1:207597/1‑150 (MQ=255)
                                                                                                                                                ttAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAAcc       <  2:3133924/150‑1 (MQ=255)
                                                                                                                                                 tAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACca      >  2:1511553/1‑150 (MQ=255)
                                                                                                                                                  aaaCGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACcag     >  1:3897137/1‑150 (MQ=255)
                                                                                                                                                    aCGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACcagca   <  2:2772330/150‑1 (MQ=255)
                                                                                                                                                     cGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACcagcag  >  1:410476/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AAACGTAATATCTTGCGATGGTTGTGTGGCGAAATTGTTTTAAAACGAATACTCCACACCATGCCAATAATGATCACAATATAGACAAACAGCATAGTTGAAGTGACGTATTTACTGGCATCCAGCGACTGACCAAACAGATAGTTAAACGCCGTGAGTCCCGCGCCAATCCCTAAGGCAATCATCAA‑TTTTTTAACGCTGATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAG  >  CP009273/2124010‑2124308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A