Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,503,015 C→A 14.5% intergenic (‑110/‑94) glk ← / → yfeO glucokinase/putative ion channel protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,503,0150CA14.5% 942.3 / 95.0 290intergenic (‑110/‑94)glk/yfeOglucokinase/putative ion channel protein
Reads supporting (aligned to +/- strand):  ref base C (132/115);  new base A (23/20);  total (155/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 307 total aligned reads displayed.

TGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAAT  >  CP009273/2502866‑2503164
                                                                                                                                                     |                                                                                                                                                     
tGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTc                                                                                                                                                       >  1:1022401/1‑150 (MQ=255)
  gTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTcac                                                                                                                                                     >  1:4214963/1‑150 (MQ=255)
  gTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTcac                                                                                                                                                     >  2:2725290/1‑150 (MQ=255)
    gccgccCACCTCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTcacac                                                                                                                                                   <  1:1423118/150‑1 (MQ=255)
     ccgccCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTcacaca                                                                                                                                                  >  1:1786126/1‑150 (MQ=255)
     ccgccCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTcacaca                                                                                                                                                  <  1:4667761/150‑1 (MQ=255)
        cccACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAAtt                                                                                                                                               <  2:5019150/150‑1 (MQ=255)
             aTCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACttt                                                                                                                                          >  2:1295047/1‑150 (MQ=255)
               cACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTAt                                                                                                                                        <  2:4638059/150‑1 (MQ=255)
                aCCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATc                                                                                                                                       <  2:5132677/150‑1 (MQ=255)
                  cGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGt                                                                                                                                     >  1:3291813/1‑150 (MQ=255)
                   gACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGtt                                                                                                                                    >  2:796283/1‑150 (MQ=255)
                   gACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGtt                                                                                                                                    <  1:3098421/150‑1 (MQ=255)
                   gACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGtt                                                                                                                                    >  2:2546250/1‑150 (MQ=255)
                     cTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTc                                                                                                                                  <  1:4450506/150‑1 (MQ=255)
                      tAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCa                                                                                                                                 >  1:337134/1‑150 (MQ=255)
                      tAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCa                                                                                                                                 >  2:2579799/1‑150 (MQ=255)
                         tGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCa                                                                                                                              <  1:4281324/150‑1 (MQ=255)
                          gCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACACTTACTTTATCGTTTCAGCAc                                                                                                                             >  2:3033483/1‑150 (MQ=255)
                          gCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCAc                                                                                                                             <  1:3021995/150‑1 (MQ=255)
                           cATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCAcc                                                                                                                            <  2:4768869/150‑1 (MQ=255)
                             tACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTAACACAATTACTTTATCGTTTCAGCACCaa                                                                                                                          <  2:1120424/150‑1 (MQ=255)
                              aCTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAAt                                                                                                                         <  2:4892252/150‑1 (MQ=255)
                              aCTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTAACACAATTACTTTATCGTTTCAGCACCAAt                                                                                                                         <  1:941147/150‑1 (MQ=255)
                                tttGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTAACACAATTACTTTATCGTTTCAGCACCAATTg                                                                                                                       >  1:2744786/1‑150 (MQ=255)
                                  tGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCa                                                                                                                     <  1:1321730/150‑1 (MQ=255)
                                   gTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAg                                                                                                                    >  2:932560/1‑150 (MQ=255)
                                     cATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCg                                                                                                                  >  2:382021/1‑150 (MQ=255)
                                      aTTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGa                                                                                                                 >  1:4970763/1‑150 (MQ=255)
                                      aTTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGa                                                                                                                 >  1:1720529/1‑150 (MQ=255)
                                         cttcAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGc                                                                                                              <  2:1033254/150‑1 (MQ=255)
                                          ttcAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGcc                                                                                                             <  1:876418/150‑1 (MQ=255)
                                            cAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCtt                                                                                                           >  2:123900/1‑150 (MQ=255)
                                            cAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCtt                                                                                                           >  2:1473561/1‑150 (MQ=255)
                                            cAACTGCTCCGCTAAAGTCAAAATAATTCTTTATCACACTGTAAATACCTTGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCtt                                                                                                           >  1:534018/1‑150 (MQ=255)
                                               cTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTAACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCttttt                                                                                                        <  1:364389/150‑1 (MQ=255)
                                                tGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTg                                                                                                       >  2:4716472/1‑150 (MQ=255)
                                                tGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTAACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCtttttt                                                                                                       >  1:781369/1‑149 (MQ=255)
                                                      gCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCt                                                                                                 <  1:2860192/150‑1 (MQ=255)
                                                       cTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTAACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTg                                                                                                >  2:3110349/1‑150 (MQ=255)
                                                       cTAAAGTCAAAATAATTCTTTCTCACACTGTAAATAACTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTAACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTg                                                                                                >  2:1609727/1‑150 (MQ=255)
                                                        tAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTgg                                                                                               >  2:1841141/1‑150 (MQ=255)
                                                        tAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTgg                                                                                               >  2:4501038/1‑150 (MQ=255)
                                                        tAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTAACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTgg                                                                                               >  2:1954182/1‑150 (MQ=255)
                                                               aaaaTAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACt                                                                                        >  1:2621085/1‑150 (MQ=255)
                                                                aaaTAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTAACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTa                                                                                       >  2:1551516/1‑150 (MQ=255)
                                                                     atTCTTTCTCACACTGTAAATACCTGGGTGGATAACAACTTAGCCGGAAAGGCAGTCTCACTGTAAATATCGATATTTGTAACAATATTAGTTTATATTTTCAGCACCAATTTGAGTTATTCCTTTTTGCATGATGTGCAAACtttttat                                                                                  <  2:1011104/150‑7 (MQ=255)
                                                                       tctttctCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCt                                                                                <  2:4481143/150‑1 (MQ=255)
                                                                         tttctCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGa                                                                              <  2:2617749/150‑1 (MQ=255)
                                                                           tctcACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGAcc                                                                            <  1:3183058/150‑1 (MQ=255)
                                                                            ctcACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGAccc                                                                           >  1:603446/1‑150 (MQ=255)
                                                                              cacaCTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCg                                                                         >  2:336476/1‑150 (MQ=255)
                                                                              cacaCTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCg                                                                         <  1:3795751/150‑1 (MQ=255)
                                                                              cacaCTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTAACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCACACTAAGTATCTGACCCCg                                                                         >  1:1121091/1‑150 (MQ=255)
                                                                               acacTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGc                                                                        >  1:3467889/1‑150 (MQ=255)
                                                                                cacTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCa                                                                       >  1:1885326/1‑150 (MQ=255)
                                                                                 acTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCAt                                                                      <  2:4445906/150‑1 (MQ=255)
                                                                                   tGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGTCAAACTAAGTATCTGACCCCGCATaa                                                                    <  2:1726618/150‑1 (MQ=255)
                                                                                    gTAAATACCTGGGGGAATAACAACAACGCCGGAAAGTCAGGCTCCCTGTAAATATCGATCTGTGTCACAAAATTACTTTATCGTTTCAGCACCAATTGCAGCTATGCCTTTTTTATAGCTGGGAAAACTAAGTATCTGACCCCGCATAAg                                                                   <  2:3276962/150‑1 (MQ=255)
                                                                                      aaaTACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGa                                                                 <  2:2979114/150‑1 (MQ=255)
                                                                                      aaaTACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGa                                                                 >  1:1046034/1‑150 (MQ=255)
                                                                                        aTACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAAt                                                               >  2:518947/1‑150 (MQ=255)
                                                                                        aTACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAAt                                                               >  1:2243610/1‑150 (MQ=255)
                                                                                        aTACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAAt                                                               >  2:1742529/1‑150 (MQ=255)
                                                                                           ccTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGa                                                            <  2:3180885/150‑1 (MQ=255)
                                                                                             tGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAAc                                                          <  2:824420/150‑1 (MQ=255)
                                                                                             tGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAAc                                                          >  1:401959/1‑150 (MQ=255)
                                                                                                gggCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAAATGCAGCGATGCATTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATg                                                       >  2:2938659/1‑150 (MQ=255)
                                                                                                 ggCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGc                                                      <  2:2255991/150‑1 (MQ=255)
                                                                                                      aacaacaacGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCAt                                                 >  1:4094524/1‑150 (MQ=255)
                                                                                                         aacaacGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTTCAGCGATGCCTTTATGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCcg                                              >  1:455086/1‑150 (MQ=255)
                                                                                                           caacGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCcgcg                                            >  2:252259/1‑150 (MQ=255)
                                                                                                            aacGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGa                                           <  1:3913640/150‑1 (MQ=255)
                                                                                                                ccGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCa                                       >  1:2231221/1‑150 (MQ=255)
                                                                                                                ccGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCa                                       <  1:135306/150‑1 (MQ=255)
                                                                                                                  ggAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGa                                     >  1:3983276/1‑150 (MQ=255)
                                                                                                                     aaGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAAcc                                  <  1:4810653/150‑1 (MQ=255)
                                                                                                                       ggcaggcTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCAt                                >  1:228540/1‑150 (MQ=255)
                                                                                                                        gcaggcTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCAtg                               >  2:771712/1‑150 (MQ=255)
                                                                                                                         caggcTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCAtgt                              >  1:1546653/1‑150 (MQ=255)
                                                                                                                          aggcTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCAtgtt                             >  1:722834/1‑150 (MQ=255)
                                                                                                                             cTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATgttgtt                          <  1:299247/150‑1 (MQ=255)
                                                                                                                             cTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATgttgtt                          <  2:3912892/150‑1 (MQ=255)
                                                                                                                                ccTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGttatt                       >  2:1653758/1‑150 (MQ=255)
                                                                                                                                  tGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTtattat                     <  2:4246263/150‑1 (MQ=255)
                                                                                                                                  tGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTtattat                     >  1:5036085/1‑150 (MQ=255)
                                                                                                                                    tAAATATCGATCTGGGTAACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCg                   <  1:4043408/150‑1 (MQ=255)
                                                                                                                                      aaTATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCt                 >  2:563708/1‑150 (MQ=255)
                                                                                                                                       atatCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTc                <  1:1295047/150‑1 (MQ=255)
                                                                                                                                         atCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTccc              >  1:2419915/1‑150 (MQ=255)
                                                                                                                                         atCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTccc              >  1:2039615/1‑150 (MQ=255)
                                                                                                                                         atCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTccc              <  1:4608764/150‑1 (MQ=255)
                                                                                                                                         atCGATCTGGGTAACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTccc              >  2:2384698/1‑150 (MQ=255)
                                                                                                                                              tCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCccgccg         >  2:683590/1‑150 (MQ=255)
                                                                                                                                              tCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCccgccg         >  1:4897279/1‑150 (MQ=255)
                                                                                                                                               cTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGt        >  1:1654727/1‑150 (MQ=255)
                                                                                                                                                tGGGTAACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTg       >  1:1469925/1‑150 (MQ=255)
                                                                                                                                                tGGGTAACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTg       >  2:4152376/1‑150 (MQ=255)
                                                                                                                                                    tCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCaa   >  2:4966021/1‑150 (MQ=255)
                                                                                                                                                    tCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCaa   >  2:2207810/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGTGCCGCCCACATCACCGACTAATGCATACTTTGTCATTCTTCAACTGCTCCGCTAAAGTCAAAATAATTCTTTCTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAAT  >  CP009273/2502866‑2503164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A