Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,041,026 C→A 14.5% D459Y (GAC→TAC)  gcvP ← glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,041,0260CA14.5% 1043.3 / 108.1 320D459Y (GAC→TAC) gcvPglycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base C (137/136);  new base A (28/19);  total (165/155)
Fisher's exact test for biased strand distribution p-value = 2.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 321 total aligned reads displayed.

CGGGATCATCGCCTGATTCAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGCTACGCAGGTTGA  >  CP009273/3040877‑3041172
                                                                                                                                                     |                                                                                                                                                  
cGGGATCATCGCCTGATTCAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTc                                                                                                                                                    >  2:2392906/1‑150 (MQ=255)
 gggATCATCGCCTGATTCAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCg                                                                                                                                                   <  2:1090431/150‑1 (MQ=255)
 gggATCATCGCCTGATTCAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCg                                                                                                                                                   <  2:4796938/150‑1 (MQ=255)
  ggATCATCGCCTGATTCAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTAGt                                                                                                                                                  >  1:3979605/1‑150 (MQ=255)
    atcatcGCCTGATTCAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGa                                                                                                                                                >  2:1295218/1‑150 (MQ=255)
     tcatcGCCTGATTCAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAg                                                                                                                                               >  2:2039205/1‑150 (MQ=255)
      catcGCCTGATTCAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGc                                                                                                                                              >  2:528386/1‑150 (MQ=255)
      catcGCCTGATTCAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGc                                                                                                                                              >  2:4164245/1‑150 (MQ=255)
       atcGCCTGATTCAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTAGTGAGcc                                                                                                                                             >  1:1507968/1‑150 (MQ=255)
         cGCCTGATTCAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCAc                                                                                                                                           <  1:1030469/150‑1 (MQ=255)
         cGCCTGATTCAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCAc                                                                                                                                           <  2:2550547/150‑1 (MQ=255)
            cTGATTCAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTc                                                                                                                                        <  1:4642940/150‑1 (MQ=255)
            cTGATTCAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTc                                                                                                                                        >  2:864255/1‑150 (MQ=255)
            cTGATTCAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTc                                                                                                                                        >  1:4275974/1‑150 (MQ=255)
             tGATTCAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCt                                                                                                                                       <  2:5039196/150‑1 (MQ=255)
                 tCAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGt                                                                                                                                   <  1:922472/150‑1 (MQ=255)
                  cAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTc                                                                                                                                  <  2:1475581/150‑1 (MQ=255)
                   aGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTcc                                                                                                                                 >  1:5031380/1‑150 (MQ=255)
                     cgcCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAg                                                                                                                               >  1:3714208/1‑150 (MQ=255)
                     cgcCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTAGTGAGCCACGTCTTTGTCCAg                                                                                                                               <  2:1456240/150‑1 (MQ=255)
                         aGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCgtg                                                                                                                           >  1:3059217/1‑150 (MQ=255)
                           aTCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTc                                                                                                                         <  1:534483/150‑1 (MQ=255)
                            tCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCg                                                                                                                        <  1:4298510/150‑1 (MQ=255)
                             cTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGa                                                                                                                       <  2:3352727/150‑1 (MQ=255)
                                 aCGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTc                                                                                                                   >  1:2257393/1‑150 (MQ=255)
                                  cGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTcc                                                                                                                  >  2:1414702/1‑150 (MQ=255)
                                    cTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTAGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAg                                                                                                                <  2:2648956/150‑1 (MQ=255)
                                     tCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAgg                                                                                                               >  1:938133/1‑150 (MQ=255)
                                        aGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCg                                                                                                            <  1:4686311/150‑1 (MQ=255)
                                        aGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCg                                                                                                            <  1:1968306/150‑1 (MQ=255)
                                           gAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTgg                                                                                                         <  2:1955890/150‑1 (MQ=255)
                                             gTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGtt                                                                                                       >  2:1021583/1‑150 (MQ=255)
                                              tGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTa                                                                                                      >  1:1309246/1‑150 (MQ=255)
                                               gCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTAt                                                                                                     <  1:476626/150‑1 (MQ=255)
                                                cATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATc                                                                                                    <  1:1776825/150‑1 (MQ=255)
                                                cATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATc                                                                                                    <  1:626404/150‑1 (MQ=255)
                                                cATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATc                                                                                                    >  2:2778029/1‑150 (MQ=255)
                                                cATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATc                                                                                                    <  1:1475328/150‑1 (MQ=255)
                                                 atatAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCg                                                                                                   >  2:4056236/1‑150 (MQ=255)
                                                     aGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTAGTGAGCCACGTCTTTGTCCAGCGTGTCGCTGTCCAGGCCGTGGTTATCGCCca                                                                                               <  1:3205763/150‑1 (MQ=255)
                                                        gcATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTAGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCcagca                                                                                            >  1:322818/1‑150 (MQ=255)
                                                           tcatTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAgcagca                                                                                         >  1:3357766/1‑150 (MQ=255)
                                                           tcatTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAgcagca                                                                                         <  2:4009345/150‑1 (MQ=255)
                                                           tcatTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTAGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAgcagca                                                                                         >  1:2868067/1‑150 (MQ=255)
                                                            catTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCAc                                                                                        >  2:2074528/1‑150 (MQ=255)
                                                              tttCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGt                                                                                      <  2:2316015/150‑1 (MQ=255)
                                                                    tttCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTAGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGaaaa                                                                                <  2:3114574/150‑1 (MQ=255)
                                                                          tgtgGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCa                                                                          >  2:1955930/1‑150 (MQ=255)
                                                                           gtgGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCAt                                                                         >  1:4423532/1‑150 (MQ=255)
                                                                             ggTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTAGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTa                                                                       >  2:3851904/1‑150 (MQ=255)
                                                                              gTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTAGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTAc                                                                      >  2:1891149/1‑150 (MQ=255)
                                                                               tAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTAGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACg                                                                     <  1:1832124/150‑1 (MQ=255)
                                                                                aGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGt                                                                    <  1:2398805/150‑1 (MQ=255)
                                                                                 gCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGtt                                                                   <  2:382388/150‑1 (MQ=255)
                                                                                  cGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGttt                                                                  >  1:1110946/1‑150 (MQ=255)
                                                                                   gATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGtttt                                                                 >  2:1766604/1‑150 (MQ=255)
                                                                                   gATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGtttt                                                                 <  2:3384091/150‑1 (MQ=255)
                                                                                      tAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCAc                                                              <  1:4949721/150‑1 (MQ=255)
                                                                                      tAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTAGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCAc                                                              <  1:2300643/150‑1 (MQ=255)
                                                                                           acCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTAGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTg                                                         >  2:1643257/1‑150 (MQ=255)
                                                                                             cGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGgt                                                       >  1:4304109/1‑150 (MQ=255)
                                                                                             cGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGgt                                                       >  1:4495015/1‑150 (MQ=255)
                                                                                              ggATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGgtt                                                      <  2:94258/150‑1 (MQ=255)
                                                                                              ggATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGgtt                                                      >  2:3551876/1‑150 (MQ=255)
                                                                                                 tGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGgttgtt                                                   >  1:1783090/1‑150 (MQ=255)
                                                                                                   ggTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTtc                                                 <  1:2560242/150‑1 (MQ=255)
                                                                                                     tGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTtcat                                               <  2:3446408/150‑1 (MQ=255)
                                                                                                     tGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTtcat                                               <  2:2952991/150‑1 (MQ=255)
                                                                                                     tGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTtcat                                               <  1:892062/150‑1 (MQ=255)
                                                                                                     tGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTtcat                                               >  1:4558842/1‑150 (MQ=255)
                                                                                                       aGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTtcatca                                             >  2:455279/1‑150 (MQ=255)
                                                                                                        ggATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCaa                                            >  1:484141/1‑150 (MQ=255)
                                                                                                           tttCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTAGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAggg                                         >  1:5099762/1‑150 (MQ=255)
                                                                                                            ttCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGt                                        >  1:2034671/1‑150 (MQ=255)
                                                                                                            ttCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTGGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGt                                        <  2:4482035/150‑1 (MQ=255)
                                                                                                              cgtcgtcgCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGa                                      >  2:4850588/1‑150 (MQ=255)
                                                                                                              cgtcgtcgCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGa                                      <  2:1548011/150‑1 (MQ=255)
                                                                                                               gtcgtcgCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGAt                                     >  2:4117085/1‑150 (MQ=255)
                                                                                                                tcgtcgCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATc                                    <  2:1242244/150‑1 (MQ=255)
                                                                                                                 cgtcgCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATcc                                   >  1:602302/1‑150 (MQ=255)
                                                                                                                    cgcgCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCaa                                <  1:1656159/150‑1 (MQ=255)
                                                                                                                    cgcgCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCaa                                >  2:4283679/1‑150 (MQ=255)
                                                                                                                    cgcgCAGCATCGCAGGCTGGATAGAGCGGCTGTAGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCaa                                <  1:3629031/150‑1 (MQ=255)
                                                                                                                      cgcAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAAcc                              <  2:4411211/150‑1 (MQ=255)
                                                                                                                        cagcaTCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGGTGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACcgc                            >  1:3731239/1‑150 (MQ=255)
                                                                                                                        cagcaTCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACcgc                            >  1:1913912/1‑150 (MQ=255)
                                                                                                                         agcaTCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACcgcg                           >  2:679670/1‑150 (MQ=255)
                                                                                                                         agcaTCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACcgcg                           <  2:3947248/150‑1 (MQ=255)
                                                                                                                           caTCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGtt                         <  1:3721350/150‑1 (MQ=255)
                                                                                                                            aTCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTc                        >  1:2630976/1‑150 (MQ=255)
                                                                                                                            aTCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTc                        >  1:2901447/1‑150 (MQ=255)
                                                                                                                                cAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGaa                    <  2:472286/150‑1 (MQ=255)
                                                                                                                                        aTAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGCTa            >  1:4810086/1‑150 (MQ=255)
                                                                                                                                         tAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGCTAc           <  2:2202510/150‑1 (MQ=255)
                                                                                                                                         tAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGCTAc           <  2:460173/150‑1 (MQ=255)
                                                                                                                                           gagCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGCTACGc         >  1:4890441/1‑150 (MQ=255)
                                                                                                                                             gCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGCTACGCAg       <  2:3721454/150‑1 (MQ=255)
                                                                                                                                              cGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGCTACGCAgg      >  1:4319018/1‑150 (MQ=255)
                                                                                                                                              cGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGCTACGCAgg      >  2:3242064/1‑150 (MQ=255)
                                                                                                                                                  tGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGCTACGCAGGTTGa  <  1:2751917/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CGGGATCATCGCCTGATTCAGCGCCAGATCTTTACGCTCCAGCGAGTGCATATAGCGCATCATTTCGGTTTCGCTGTGGTAGCGATTAAACACCGGATGGGTGAGGATTTCGTCGTCGCGCAGCATCGCAGGCTGGATAGAGCGGCTGTCGTGAGCCACGTCTTTGTCCAGCGTGTCGATGTCCAGGCCGTGGTTATCGCCCAGCAGCACGTTGAAAAGCTGCATTACGTTTTCACGCGTGGTTGTTTCATCAAGGGTGATCCCAACCGCGTTCAGAATATCGCTACGCAGGTTGA  >  CP009273/3040877‑3041172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A