Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 619,048 C→A 74.6% A307S (GCC→TCC)  fepB ← iron‑enterobactin transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273619,0480CA74.6% 386.3 / 220.8 256A307S (GCC→TCC) fepBiron‑enterobactin transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (30/35);  new base A (91/100);  total (121/135)
Fisher's exact test for biased strand distribution p-value = 8.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01

Only 100 of 259 total aligned reads displayed.

AGCGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCATTT  >  CP009273/618902‑619196
                                                                                                                                                  |                                                                                                                                                    
aGCGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCt                                                                                                                                                   >  1:2938022/1‑150 (MQ=255)
  cGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTgt                                                                                                                                                 <  2:2702464/150‑1 (MQ=255)
   ggTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTgta                                                                                                                                                >  1:3674888/1‑150 (MQ=255)
   ggTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTgta                                                                                                                                                <  2:1971525/150‑1 (MQ=255)
        tGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAAt                                                                                                                                           <  1:4166305/150‑1 (MQ=255)
         ggTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGAAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATc                                                                                                                                          >  2:4134820/1‑150 (MQ=255)
           tttGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCa                                                                                                                                        <  2:59596/150‑1 (MQ=255)
           tttGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCa                                                                                                                                        <  2:398913/150‑1 (MQ=255)
            ttgttgATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAg                                                                                                                                       >  1:2632959/1‑150 (MQ=255)
            ttgttgATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGATTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAg                                                                                                                                       <  2:1768660/150‑1 (MQ=255)
                 gATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGa                                                                                                                                  >  1:2661261/1‑150 (MQ=255)
                  aTTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGaa                                                                                                                                 <  2:3388935/150‑1 (MQ=255)
                   ttATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAAc                                                                                                                                <  2:3750909/150‑1 (MQ=255)
                   ttATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAAc                                                                                                                                <  2:1379219/150‑1 (MQ=255)
                           gtgtTTTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGt                                                                                                                        >  1:3910474/1‑150 (MQ=255)
                           gtgtTGTTAGTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGt                                                                                                                        <  1:1106542/150‑1 (MQ=255)
                            tgttgtTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGtt                                                                                                                       >  1:817830/1‑150 (MQ=255)
                              ttgttATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTcc                                                                                                                     <  2:281522/150‑1 (MQ=255)
                              ttgttATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTcc                                                                                                                     <  2:781925/150‑1 (MQ=255)
                               tgttATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTccc                                                                                                                    <  1:527107/150‑1 (MQ=255)
                               tgttATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTccc                                                                                                                    <  1:1318976/150‑1 (MQ=255)
                                  tATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAgc                                                                                                                 >  2:3412025/1‑150 (MQ=255)
                                   aTTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAgcg                                                                                                                >  2:3015383/1‑150 (MQ=255)
                                     tgctggtgctggTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCa                                                                                                              >  1:2759013/1‑150 (MQ=255)
                                      gctggtgctggtGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCAt                                                                                                             >  1:3251294/1‑150 (MQ=255)
                                       ctggtgctggtgGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATa                                                                                                            <  1:2528098/150‑1 (MQ=255)
                                        tggtgctggtgGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATaa                                                                                                           >  1:705265/1‑150 (MQ=255)
                                        tggtgctggtgGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGACTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATaa                                                                                                           <  1:2497189/150‑1 (MQ=255)
                                         ggtgctggtgGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATaaa                                                                                                          >  1:2147600/1‑150 (MQ=255)
                                          gtgctggtgGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAAc                                                                                                         <  2:181377/150‑1 (MQ=255)
                                              tggtggAGTTGCGACATTTTCGCCCGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGc                                                                                                     >  2:1323959/1‑150 (MQ=255)
                                              tggtggAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTAGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGc                                                                                                     >  2:3596124/1‑150 (MQ=255)
                                                 tggAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTg                                                                                                  >  1:2912025/1‑150 (MQ=255)
                                                   gAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCAACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGAATCGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGtt                                                                                                >  1:1021097/1‑150 (MQ=255)
                                                      ttGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTg                                                                                             <  2:847285/150‑1 (MQ=255)
                                                          gACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACt                                                                                         <  2:3456301/150‑1 (MQ=255)
                                                          gACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACt                                                                                         <  2:314667/150‑1 (MQ=255)
                                                             aTTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTgca                                                                                      >  1:1670865/1‑150 (MQ=255)
                                                               tttCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTgcagg                                                                                    >  1:4412359/1‑150 (MQ=255)
                                                                 tCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTgcaggca                                                                                  <  1:1221934/150‑1 (MQ=255)
                                                                  cGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTgcaggcag                                                                                 >  1:2758855/1‑150 (MQ=255)
                                                                    ccAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGt                                                                               <  1:3904442/150‑1 (MQ=255)
                                                                      aGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTgc                                                                             <  2:1406548/150‑1 (MQ=255)
                                                                      aGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTgc                                                                             >  2:4101286/1‑150 (MQ=255)
                                                                      aGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTgc                                                                             <  1:2817222/150‑1 (MQ=255)
                                                                         cgccgccgcAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGcgcg                                                                          <  1:3043740/150‑1 (MQ=255)
                                                                          gccgccgcAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGa                                                                         <  2:4315277/150‑1 (MQ=255)
                                                                            cgccgcAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGagc                                                                       <  1:4206623/150‑1 (MQ=255)
                                                                            cgccgcAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGagc                                                                       <  2:3027906/150‑1 (MQ=255)
                                                                            cgccgcAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGagc                                                                       <  2:1894224/150‑1 (MQ=255)
                                                                             gccgcAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGTGCGagca                                                                      <  2:2465684/150‑1 (MQ=255)
                                                                              ccgcAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGagcag                                                                     <  2:2379804/150‑1 (MQ=255)
                                                                                  aGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCGTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGa                                                                 >  1:3454798/1‑150 (MQ=255)
                                                                                   ggTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGAt                                                                <  1:4520468/150‑1 (MQ=255)
                                                                                   ggTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGAt                                                                <  2:288996/150‑1 (MQ=255)
                                                                                    gTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGAtt                                                               <  2:2880824/150‑1 (MQ=255)
                                                                                    gTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGAtt                                                               <  2:269897/150‑1 (MQ=255)
                                                                                     tGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTa                                                              >  1:361013/1‑150 (MQ=255)
                                                                                     tGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTa                                                              <  1:941350/150‑1 (MQ=255)
                                                                                      gACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAg                                                             <  1:1327966/150‑1 (MQ=255)
                                                                                         aGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCAt                                                          >  2:2000830/1‑150 (MQ=255)
                                                                                          gCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATa                                                         <  2:1692050/150‑1 (MQ=255)
                                                                                            gTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATaaa                                                       <  1:3400279/150‑1 (MQ=255)
                                                                                              ccGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATa                                                     >  2:802851/1‑150 (MQ=255)
                                                                                               cGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAg                                                    <  2:534266/150‑1 (MQ=255)
                                                                                               cGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAg                                                    <  1:1506605/150‑1 (MQ=255)
                                                                                                  cAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCAt                                                 >  1:1649617/1‑150 (MQ=255)
                                                                                                  cAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCAt                                                 >  2:1746690/1‑150 (MQ=255)
                                                                                                  cAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCAt                                                 <  2:1597966/150‑1 (MQ=255)
                                                                                                  cAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCAt                                                 <  1:774587/150‑1 (MQ=255)
                                                                                                       aaTGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCa                                            >  2:2007345/1‑150 (MQ=255)
                                                                                                       aaTGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCa                                            <  2:3934868/150‑1 (MQ=255)
                                                                                                        aTGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCAt                                           >  2:484353/1‑150 (MQ=255)
                                                                                                         tGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATc                                          <  2:3287007/150‑1 (MQ=255)
                                                                                                         tGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATc                                          <  2:3287043/150‑1 (MQ=255)
                                                                                                          gCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCt                                         <  2:636434/150‑1 (MQ=255)
                                                                                                          gCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCt                                         <  2:812194/150‑1 (MQ=255)
                                                                                                           cTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCtt                                        >  1:3963293/1‑150 (MQ=255)
                                                                                                              aaaaCAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCt                                     >  1:3716541/1‑150 (MQ=255)
                                                                                                              aaaaCAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCt                                     >  1:3315704/1‑150 (MQ=255)
                                                                                                                aaCAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGa                                   >  2:4217549/1‑150 (MQ=255)
                                                                                                                 aCAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGAt                                  <  1:517786/150‑1 (MQ=255)
                                                                                                                 aCAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGAt                                  >  2:1592422/1‑150 (MQ=255)
                                                                                                                  cAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATc                                 >  2:1209159/1‑150 (MQ=255)
                                                                                                                  cAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATc                                 <  2:2300920/150‑1 (MQ=255)
                                                                                                                    gcgcCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCAc                               <  2:384119/150‑1 (MQ=255)
                                                                                                                        cTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGc                           <  1:1976733/150‑1 (MQ=255)
                                                                                                                             gCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACa                      <  1:1732494/150‑1 (MQ=255)
                                                                                                                              ccTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAg                     >  1:1205979/1‑150 (MQ=255)
                                                                                                                                   ccAGCACTTGCATGGCGATGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATa                >  2:3662221/1‑150 (MQ=255)
                                                                                                                                   ccAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATa                <  2:2553921/150‑1 (MQ=255)
                                                                                                                                        aCTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGAc           <  2:103301/150‑1 (MQ=255)
                                                                                                                                        aCTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGAc           >  1:3798642/1‑150 (MQ=255)
                                                                                                                                        aCTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGAc           >  1:1839307/1‑150 (MQ=255)
                                                                                                                                           tGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCg        >  2:698862/1‑150 (MQ=255)
                                                                                                                                             cATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGcc      >  1:1999734/1‑150 (MQ=255)
                                                                                                                                               tGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCAt    >  1:2531963/1‑150 (MQ=255)
                                                                                                                                               tGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCAt    <  2:4140967/150‑1 (MQ=255)
                                                                                                                                                ggCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCAtt   <  1:1382084/150‑1 (MQ=255)
                                                                                                                                                 gcgcTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCAttt  <  1:251538/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AGCGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCATTT  >  CP009273/618902‑619196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCATTTA  >  CP009273/618902‑619197
                                                                                                                                                  |                                                                                                                                                     
AGCGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCT                                                                                                                                                    >  SRR19973076.2945325/1‑150 (MQ=60)
AGCGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCT                                                                                                                                                    >  SRR19973076.2942287/1‑150 (MQ=60)
 GCGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTG                                                                                                                                                   <  SRR19973076.2802649/150‑1 (MQ=60)
  CGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGTTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGT                                                                                                                                                  >  SRR19973076.4459126/1‑150 (MQ=60)
  CGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGT                                                                                                                                                  <  SRR19973076.3393385/150‑1 (MQ=60)
  CGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGT                                                                                                                                                  <  SRR19973076.3173631/150‑1 (MQ=60)
  CGGTTTTGGTTTGTTGATTATCGGCGTGTTGTAATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGCTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGT                                                                                                                                                  >  SRR19973076.4458715/1‑150 (MQ=60)
   GGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTA                                                                                                                                                 >  SRR19973076.3680254/1‑150 (MQ=60)
   GGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTA                                                                                                                                                 >  SRR19973076.1408624/1‑150 (MQ=60)
        TGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAAT                                                                                                                                            <  SRR19973076.4172459/150‑1 (MQ=60)
            TTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAG                                                                                                                                        >  SRR19973076.2636793/1‑150 (MQ=60)
            TTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAG                                                                                                                                        >  SRR19973076.2636782/1‑150 (MQ=60)
                 GATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGA                                                                                                                                   >  SRR19973076.2665130/1‑150 (MQ=60)
                  ATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAA                                                                                                                                  >  SRR19973076.4064589/1‑150 (MQ=60)
                  ATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAA                                                                                                                                  <  SRR19973076.1114463/150‑1 (MQ=60)
                        GGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTC                                                                                                                            <  SRR19973076.3295749/150‑1 (MQ=60)
                         GCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCG                                                                                                                           <  SRR19973076.561808/150‑1 (MQ=60)
                           GTGTTTTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGT                                                                                                                         >  SRR19973076.3916255/1‑150 (MQ=60)
                           GTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGT                                                                                                                         >  SRR19973076.3915095/1‑150 (MQ=60)
                           GTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGT                                                                                                                         >  SRR19973076.3916394/1‑150 (MQ=60)
                           GTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGT                                                                                                                         <  SRR19973076.1106706/150‑1 (MQ=60)
                           GTGTTGTTAGTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGT                                                                                                                         <  SRR19973076.1108178/150‑1 (MQ=60)
                            TGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTT                                                                                                                        >  SRR19973076.819449/1‑150 (MQ=60)
                            TGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTT                                                                                                                        >  SRR19973076.819034/1‑150 (MQ=60)
                               TGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCC                                                                                                                     <  SRR19973076.1320710/150‑1 (MQ=60)
                               TGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCC                                                                                                                     <  SRR19973076.1320916/150‑1 (MQ=60)
                               TGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCC                                                                                                                     <  SRR19973076.527945/150‑1 (MQ=60)
                                 TTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAG                                                                                                                   >  SRR19973076.2670960/1‑150 (MQ=60)
                                 TTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAG                                                                                                                   >  SRR19973076.2666919/1‑150 (MQ=60)
                                     TGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCA                                                                                                               >  SRR19973076.2763019/1‑150 (MQ=60)
                                      GCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCAT                                                                                                              >  SRR19973076.3256022/1‑150 (MQ=60)
                                       CTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATA                                                                                                             <  SRR19973076.2531920/150‑1 (MQ=60)
                                       CTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATA                                                                                                             <  SRR19973076.2531778/150‑1 (MQ=60)
                                        TGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAA                                                                                                            >  SRR19973076.706318/1‑150 (MQ=60)
                                        TGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAA                                                                                                            <  SRR19973076.2500832/150‑1 (MQ=60)
                                        TGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGACTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAA                                                                                                            <  SRR19973076.2500819/150‑1 (MQ=60)
                                         GGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAA                                                                                                           >  SRR19973076.2150712/1‑150 (MQ=60)
                                               GGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCT                                                                                                     >  SRR19973076.1812366/1‑150 (MQ=60)
                                                 TGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTG                                                                                                   >  SRR19973076.251990/1‑150 (MQ=60)
                                                 TGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTG                                                                                                   >  SRR19973076.2916259/1‑150 (MQ=60)
                                                   GAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTT                                                                                                 >  SRR19973076.16398/1‑150 (MQ=60)
                                                   GAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCAACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGAATCGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTT                                                                                                 >  SRR19973076.1022618/1‑150 (MQ=60)
                                                          GACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACT                                                                                          >  SRR19973076.2658354/1‑150 (MQ=60)
                                                            CATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGC                                                                                        <  SRR19973076.2868303/150‑1 (MQ=60)
                                                             ATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCA                                                                                       >  SRR19973076.1673307/1‑150 (MQ=60)
                                                             ATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCA                                                                                       >  SRR19973076.1694585/1‑150 (MQ=60)
                                                               TTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGG                                                                                     >  SRR19973076.4418904/1‑150 (MQ=60)
                                                                 TCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCA                                                                                   <  SRR19973076.1223728/150‑1 (MQ=60)
                                                                 TCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCA                                                                                   <  SRR19973076.1223745/150‑1 (MQ=60)
                                                                  CGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAG                                                                                  >  SRR19973076.2762861/1‑150 (MQ=60)
                                                                  CGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAG                                                                                  >  SRR19973076.2762901/1‑150 (MQ=60)
                                                                    CCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGT                                                                                <  SRR19973076.3910216/150‑1 (MQ=60)
                                                                      AGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGC                                                                              <  SRR19973076.2821305/150‑1 (MQ=60)
                                                                      AGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGC                                                                              <  SRR19973076.3802130/150‑1 (MQ=60)
                                                                         CGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCG                                                                           <  SRR19973076.3048170/150‑1 (MQ=60)
                                                                           CCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAG                                                                         >  SRR19973076.360458/1‑150 (MQ=60)
                                                                           CCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAG                                                                         >  SRR19973076.2139499/1‑150 (MQ=60)
                                                                            CGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGC                                                                        <  SRR19973076.4213338/150‑1 (MQ=60)
                                                                            CGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGC                                                                        <  SRR19973076.4212834/150‑1 (MQ=60)
                                                                            CGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGC                                                                        <  SRR19973076.4212835/150‑1 (MQ=60)
                                                                               CGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATTCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGC                                                                     >  SRR19973076.2941790/1‑150 (MQ=60)
                                                                                  AGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGA                                                                  >  SRR19973076.3459782/1‑150 (MQ=60)
                                                                                  AGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGA                                                                  >  SRR19973076.3459802/1‑150 (MQ=60)
                                                                                  AGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCGTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGA                                                                  >  SRR19973076.3459795/1‑150 (MQ=60)
                                                                                   GGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGAT                                                                 <  SRR19973076.4528140/150‑1 (MQ=60)
                                                                                   GGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGAT                                                                 <  SRR19973076.4527171/150‑1 (MQ=60)
                                                                                     TGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTA                                                               >  SRR19973076.361579/1‑150 (MQ=60)
                                                                                     TGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTA                                                               >  SRR19973076.361587/1‑150 (MQ=60)
                                                                                     TGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTA                                                               <  SRR19973076.942742/150‑1 (MQ=60)
                                                                                     TGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTA                                                               <  SRR19973076.942830/150‑1 (MQ=60)
                                                                                      GACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAG                                                              <  SRR19973076.1329917/150‑1 (MQ=60)
                                                                                      GACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAG                                                              <  SRR19973076.1655212/150‑1 (MQ=60)
                                                                                      GACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAG                                                              <  SRR19973076.1329892/150‑1 (MQ=60)
                                                                                      GACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAG                                                              <  SRR19973076.1655393/150‑1 (MQ=60)
                                                                                        CAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCA                                                            <  SRR19973076.2371909/150‑1 (MQ=60)
                                                                                            GTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAA                                                        <  SRR19973076.3405206/150‑1 (MQ=60)
                                                                                            GTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTAATGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAA                                                        >  SRR19973076.1370970/1‑150 (MQ=60)
                                                                                               CGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAG                                                     <  SRR19973076.2890270/150‑1 (MQ=60)
                                                                                               CGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAG                                                     <  SRR19973076.1508819/150‑1 (MQ=60)
                                                                                                  CAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCAT                                                  >  SRR19973076.3914209/1‑150 (MQ=60)
                                                                                                  CAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCAT                                                  <  SRR19973076.775719/150‑1 (MQ=60)
                                                                                                  CAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCAT                                                  <  SRR19973076.775724/150‑1 (MQ=60)
                                                                                                  CAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCAT                                                  <  SRR19973076.775725/150‑1 (MQ=60)
                                                                                                  CAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCAT                                                  <  SRR19973076.775553/150‑1 (MQ=60)
                                                                                                  CAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCAT                                                  >  SRR19973076.1652031/1‑150 (MQ=60)
                                                                                                   AGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATC                                                 >  SRR19973076.1275455/1‑150 (MQ=60)
                                                                                                   AGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATC                                                 >  SRR19973076.374710/1‑150 (MQ=60)
                                                                                                         TGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATC                                           <  SRR19973076.2782001/150‑1 (MQ=60)
                                                                                                           CTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTT                                         >  SRR19973076.3969129/1‑150 (MQ=60)
                                                                                                              AAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCT                                      >  SRR19973076.3721989/1‑150 (MQ=60)
                                                                                                              AAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCT                                      >  SRR19973076.3721947/1‑150 (MQ=60)
                                                                                                              AAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCT                                      >  SRR19973076.3721688/1‑150 (MQ=60)
                                                                                                              AAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCT                                      >  SRR19973076.3321558/1‑150 (MQ=60)
                                                                                                              AAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCT                                      >  SRR19973076.3320520/1‑150 (MQ=60)
                                                                                                              AAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCT                                      <  SRR19973076.3842335/150‑1 (MQ=60)
                                                                                                                 ACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGAT                                   <  SRR19973076.518636/150‑1 (MQ=60)
                                                                                                                 ACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGAT                                   <  SRR19973076.518617/150‑1 (MQ=60)
                                                                                                                 ACAGCGCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCCGAT                                   <  SRR19973076.518637/150‑1 (MQ=60)
                                                                                                                      GCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCG                              >  SRR19973076.797198/1‑150 (MQ=60)
                                                                                                                      GCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCG                              >  SRR19973076.2144504/1‑150 (MQ=60)
                                                                                                                      GCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCG                              >  SRR19973076.184120/1‑150 (MQ=60)
                                                                                                                      GCCTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCG                              >  SRR19973076.3263276/1‑150 (MQ=60)
                                                                                                                        CTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTTATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGC                            <  SRR19973076.4098262/150‑1 (MQ=60)
                                                                                                                        CTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGC                            <  SRR19973076.1979601/150‑1 (MQ=60)
                                                                                                                        CTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGC                            <  SRR19973076.1979592/150‑1 (MQ=60)
                                                                                                                        CTTAAGCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGC                            <  SRR19973076.1979609/150‑1 (MQ=60)
                                                                                                                             GCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACA                       <  SRR19973076.1735008/150‑1 (MQ=60)
                                                                                                                             GCCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACA                       <  SRR19973076.1413287/150‑1 (MQ=60)
                                                                                                                              CCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAG                      >  SRR19973076.1207771/1‑150 (MQ=60)
                                                                                                                              CCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAG                      >  SRR19973076.1207681/1‑150 (MQ=60)
                                                                                                                              CCTATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAG                      <  SRR19973076.4188299/150‑1 (MQ=60)
                                                                                                                                TATCCAGCACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGA                    <  SRR19973076.2591526/150‑1 (MQ=60)
                                                                                                                                        ACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGAC            >  SRR19973076.1841936/1‑150 (MQ=60)
                                                                                                                                        ACTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGAC            >  SRR19973076.3804217/1‑150 (MQ=60)
                                                                                                                                         CTTGCATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACT           <  SRR19973076.3009104/150‑1 (MQ=60)
                                                                                                                                             CATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCC       >  SRR19973076.2002650/1‑150 (MQ=60)
                                                                                                                                             CATGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCC       >  SRR19973076.2002612/1‑150 (MQ=60)
                                                                                                                                               TGGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCAT     >  SRR19973076.2535647/1‑150 (MQ=60)
                                                                                                                                                GGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCATT    <  SRR19973076.1384124/150‑1 (MQ=60)
                                                                                                                                                GGCGCGGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGATTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCATT    <  SRR19973076.1383987/150‑1 (MQ=60)
                                                                                                                                                GGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCATT    >  SRR19973076.2913664/1‑150 (MQ=60)
                                                                                                                                                GGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCATT    >  SRR19973076.456264/1‑150 (MQ=60)
                                                                                                                                                GGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCATT    >  SRR19973076.1833611/1‑150 (MQ=60)
                                                                                                                                                GGAGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCATT    >  SRR19973076.456244/1‑150 (MQ=60)
                                                                                                                                                 GCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCATTT   <  SRR19973076.251944/150‑1 (MQ=60)
                                                                                                                                                 GCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCATTT   <  SRR19973076.251958/150‑1 (MQ=60)
                                                                                                                                                 GCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCATTT   <  SRR19973076.252036/150‑1 (MQ=60)
                                                                                                                                                  AGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGACTAGTGACTCGCCATTTA  >  SRR19973076.2826981/1‑150 (MQ=60)
                                                                                                                                                  AGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCATTTA  >  SRR19973076.537002/1‑150 (MQ=60)
                                                                                                                                                  AGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCAGCTTTCTGATCAGCGGCGAACAGGAATAGTGAGTCGCCATGTA  >  SRR19973076.2827215/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
AGCGGTTTTGGTTTGTTGATTATCGGCGTGTTGTTATTGCTGGTGCTGGTGGAGTTGCGACATTTTCGCCAGACGCCGCCGCAGGTGACAGCGTCCGACAGTTAATGCTTAAAACAGCGCCTTAAGCCTATCCAGCACTTGCATGGCGCTGTAGTAATCCAGACGGAACGTCTCGGTTCCCAGCGCATAAACCTGCTTGTTTTGTACTGCAGGCAGGTGCGCGAGCAGCGGATTAGCATAAATAGCATCGGCATCTTTCTGATCACCGGCGAACAGGAATAGTGACTCGCCATTTA  >  CP009273/618902‑619197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑