Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 629,276 A→T 43.2% K79* (AAA→TAA)  ybdL → methionine aminotransferase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273629,2760AT43.2% 137.7 / inf 257K79* (AAA→TAA) ybdLmethionine aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (55/91);  new base T (53/58);  total (108/149)
Fisher's exact test for biased strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 257 total aligned reads displayed.

CAGGCGATTAACCTGTCGCAAGGCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTA  >  CP009273/629132‑629424
                                                                                                                                                 |                                                                                                                                                    
    cGATTAACCTGTCGCAAGGCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAAc                                                                                                                                             <  2:600399/150‑1 (MQ=255)
    cGATTAACCTGTCGCAAGGCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAAc                                                                                                                                             >  2:2755720/1‑150 (MQ=255)
    cGATTAACCTGTCGCAAGGCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAAc                                                                                                                                             <  2:1501631/150‑1 (MQ=255)
        tAACCTGTCGCAAGGCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGttt                                                                                                                                         <  1:856570/150‑1 (MQ=255)
         aaCCTGTCGCAAGGCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTg                                                                                                                                        <  1:2149781/150‑1 (MQ=255)
          aCCTGTCGCAAGGCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGt                                                                                                                                       >  1:2449992/1‑150 (MQ=255)
          aCCTGTCGCAAGGCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGt                                                                                                                                       <  1:2181044/150‑1 (MQ=255)
          aCCTGTCGCAAGGCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGt                                                                                                                                       <  1:4420532/150‑1 (MQ=255)
            cTGTCGCAAGGCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTAt                                                                                                                                     <  1:3294607/150‑1 (MQ=255)
             tGTCGCAAGGCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATg                                                                                                                                    >  1:1907758/1‑150 (MQ=255)
             tGTCGCAAGGCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATg                                                                                                                                    <  2:3106379/150‑1 (MQ=255)
               tCGCAAGGCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGc                                                                                                                                  <  1:1233211/150‑1 (MQ=255)
                  cAAGGCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTAt                                                                                                                               >  1:3425776/1‑150 (MQ=255)
                   aaGGCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATc                                                                                                                              <  2:133930/150‑1 (MQ=255)
                    aGGCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCa                                                                                                                             >  2:2848613/1‑150 (MQ=255)
                     ggCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCaa                                                                                                                            >  1:3822887/1‑150 (MQ=255)
                     ggCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCaa                                                                                                                            >  1:3822883/1‑150 (MQ=255)
                       cTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAAcc                                                                                                                          >  2:3582402/1‑150 (MQ=255)
                       cTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGACAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAAcc                                                                                                                          >  1:1641346/1‑150 (MQ=255)
                        tttCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCa                                                                                                                         >  2:1871184/1‑150 (MQ=255)
                           ccTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGAt                                                                                                                      <  1:731607/150‑1 (MQ=255)
                            cTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATg                                                                                                                     <  1:281582/150‑1 (MQ=255)
                            cTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATg                                                                                                                     <  1:282156/150‑1 (MQ=255)
                             tGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGc                                                                                                                    <  2:4399704/150‑1 (MQ=255)
                              gATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGcc                                                                                                                   <  2:218939/150‑1 (MQ=255)
                               aTTTTGATGGTCCACGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCg                                                                                                                  >  2:3513366/1‑150 (MQ=255)
                                 tttGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGAt                                                                                                                <  2:2337889/150‑1 (MQ=255)
                                 tttGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCAAAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGAt                                                                                                                <  1:3208547/150‑1 (MQ=255)
                                  ttGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATa                                                                                                               <  2:2070431/150‑1 (MQ=255)
                                     aTGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCg                                                                                                            <  1:2537245/150‑1 (MQ=255)
                                     aTGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCg                                                                                                            >  1:3520630/1‑150 (MQ=255)
                                     aTGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCg                                                                                                            <  1:4023501/150‑1 (MQ=255)
                                       ggTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGat                                                                                                          >  1:3436290/1‑150 (MQ=255)
                                       ggTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGat                                                                                                          >  1:3436204/1‑150 (MQ=255)
                                        gTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGata                                                                                                         >  1:4045644/1‑150 (MQ=255)
                                          ccGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATc                                                                                                       <  2:1706479/150‑1 (MQ=255)
                                              gcTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCg                                                                                                   >  2:2893308/1‑150 (MQ=255)
                                              gcTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCg                                                                                                   >  1:852257/1‑150 (MQ=255)
                                              gcTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCg                                                                                                   >  1:2950390/1‑150 (MQ=255)
                                                tATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTa                                                                                                 <  1:2955225/150‑1 (MQ=255)
                                                tATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTa                                                                                                 >  1:3600673/1‑150 (MQ=255)
                                                tATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTa                                                                                                 <  2:3873035/150‑1 (MQ=255)
                                                tATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTa                                                                                                 <  1:919277/150‑1 (MQ=255)
                                                  tttACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAAc                                                                                               <  1:2993423/150‑1 (MQ=255)
                                                      cAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCa                                                                                           <  2:2739553/150‑1 (MQ=255)
                                                       aGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTTTATGGCTATCAACCAGATGCCGATAGCGATATCAACGTAACGGCAg                                                                                          <  2:3860113/150‑1 (MQ=255)
                                                       aGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAg                                                                                          <  2:1020679/150‑1 (MQ=255)
                                                          aGCGGCTGGCGCACCACGTTGCACAGGGGTCACACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAgggg                                                                                       >  2:3660429/1‑150 (MQ=255)
                                                           gCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGc                                                                                      <  1:2596151/150‑1 (MQ=255)
                                                            cGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCg                                                                                     <  2:251985/150‑1 (MQ=255)
                                                             ggCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGa                                                                                    >  1:2731434/1‑150 (MQ=255)
                                                              gCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGAc                                                                                   <  2:2275539/150‑1 (MQ=255)
                                                                tGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACgg                                                                                 <  1:3802170/150‑1 (MQ=255)
                                                                tGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACgg                                                                                 >  2:1687191/1‑150 (MQ=255)
                                                                  gcgcACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGaa                                                                               <  2:2170309/150‑1 (MQ=255)
                                                                   cgcACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAg                                                                              <  1:1620908/150‑1 (MQ=255)
                                                                       ccacGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGtt                                                                          <  1:1079731/150‑1 (MQ=255)
                                                                       ccacGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGtt                                                                          <  2:687972/150‑1 (MQ=255)
                                                                           gTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATAc                                                                      <  2:3175413/150‑1 (MQ=255)
                                                                           gTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATAc                                                                      >  1:3232830/1‑150 (MQ=255)
                                                                               caccGGGGGCAAACCCATTCGCCCCCCTGCACGGCCGTG‑AGGGCTTGGGGCAGGGGTTTGGGCACTAAACGGTACGGTTGTTAGGCCATCAACCATTTGGCGTTTGCGCTATCCCCGTAAAGGCAGGGGCGACGGAAGCGTTATACgcgg                                                                  <  2:145891/150‑1 (MQ=255)
                                                                                   gggggCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATAGCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGAt                                                              >  1:1721180/1‑150 (MQ=255)
                                                                                    gggtCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGAtt                                                             <  2:2620400/150‑1 (MQ=255)
                                                                                       gCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTAcc                                                          >  1:383053/1‑150 (MQ=255)
                                                                                         aaaCCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGc                                                        <  2:3347845/150‑1 (MQ=255)
                                                                                         aaaCCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGc                                                        >  1:1000756/1‑150 (MQ=255)
                                                                                            ccAATCCGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACt                                                     <  2:3194509/150‑1 (MQ=255)
                                                                                            ccAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACt                                                     <  2:1231995/150‑1 (MQ=255)
                                                                                                     gcCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCaa                                            <  2:1142555/150‑1 (MQ=255)
                                                                                                      cccATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAAt                                           >  2:1923004/1‑150 (MQ=255)
                                                                                                       ccATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATg                                          >  1:658508/1‑150 (MQ=255)
                                                                                                          tG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCg                                       <  1:2822417/150‑1 (MQ=255)
                                                                                                           g‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGa                                      >  2:3366036/1‑150 (MQ=255)
                                                                                                           g‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGa                                      <  1:2187947/150‑1 (MQ=255)
                                                                                                           g‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCTGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGa                                      <  1:986652/150‑1 (MQ=255)
                                                                                                           g‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGa                                      >  1:388464/1‑150 (MQ=255)
                                                                                                                ggCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGaa                                  <  2:401805/150‑1 (MQ=255)
                                                                                                                  cGTGCAGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGt                                <  2:3322296/150‑1 (MQ=255)
                                                                                                                   gTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTg                               <  2:3448860/150‑1 (MQ=255)
                                                                                                                       aGGCCTTGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGAttt                           >  1:2281893/1‑150 (MQ=255)
                                                                                                                       aGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGAttt                           <  1:1817416/150‑1 (MQ=255)
                                                                                                                       aGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGAttt                           <  2:4065747/150‑1 (MQ=255)
                                                                                                                          ccTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGtt                        <  1:128055/150‑1 (MQ=255)
                                                                                                                           cTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGttt                       >  2:2473763/1‑150 (MQ=255)
                                                                                                                            ttGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGtttt                      >  2:3821958/1‑150 (MQ=255)
                                                                                                                            ttGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGtttt                      >  2:3822274/1‑150 (MQ=255)
                                                                                                                             tGCGCGAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTg                     <  2:2012639/150‑1 (MQ=255)
                                                                                                                              gcgcGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGa                    <  1:353072/150‑1 (MQ=255)
                                                                                                                                gcgAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATc                  <  2:854653/150‑1 (MQ=255)
                                                                                                                                 cgAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATcc                 <  2:3425776/150‑1 (MQ=255)
                                                                                                                                  gAGGCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATccc                <  2:927678/150‑1 (MQ=255)
                                                                                                                                     gCGATTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGc             <  1:3615838/150‑1 (MQ=255)
                                                                                                                                       gATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGTAGTGATTTGTTTTGATCCCAGCTa           >  2:4210808/1‑150 (MQ=255)
                                                                                                                                        aTTGCTCAGTAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTAt          <  2:2380388/150‑1 (MQ=255)
                                                                                                                                          tGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGa        <  1:1895449/150‑1 (MQ=255)
                                                                                                                                          tGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGa        >  1:1566867/1‑150 (MQ=255)
                                                                                                                                           gCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGAc       <  1:2755690/150‑1 (MQ=255)
                                                                                                                                           gCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGAc       >  2:549171/1‑150 (MQ=255)
                                                                                                                                            cTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACa      <  2:2647742/150‑1 (MQ=255)
                                                                                                                                             tCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTGGTTTTGATCCCAGCTATGACAg     >  1:4315515/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CAGGCGATTAACCTGTCGCAAGGCTTTCCTGATTTTGATGGTCCGCGCTATTTACAGGAGCGGCTGGCGCACCACGTTGCACAGGGGGCAAACCAATACGCGCCCATG‑ACCGGCGTGCAGGCCTTGCGCGAGGCGATTGCTCAGAAAACGGAACGTTTGTATGGCTATCAACCAGATGCCGATAGCGATATCACCGTAACGGCAGGGGCGACGGAAGCGTTATACGCGGCGATTACCGCACTGGTGCGCAATGGCGATGAAGTGATTTGTTTTGATCCCAGCTATGACAGTTA  >  CP009273/629132‑629424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A