Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,808,987 G→T 6.3% S271* (TCG→TAG)  gshA ← glutamate‑cysteine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,808,9870GT 6.3% 1037.9 / 28.5 278S271* (TCG→TAG) gshAglutamate‑cysteine ligase
Reads supporting (aligned to +/- strand):  ref base G (123/136);  new base T (12/6);  total (136/142)
Fisher's exact test for biased strand distribution p-value = 1.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 281 total aligned reads displayed.

GTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCG  >  CP009273/2808838‑2809136
                                                                                                                                                     |                                                                                                                                                     
gTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCg                                                                                                                                                       >  1:3503005/1‑150 (MQ=255)
 tAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGAACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGa                                                                                                                                                      >  2:196583/1‑150 (MQ=255)
     aGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGc                                                                                                                                                  >  1:4294695/1‑150 (MQ=255)
     aGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGc                                                                                                                                                  >  1:465421/1‑150 (MQ=255)
        gcATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGtt                                                                                                                                               <  1:3604883/150‑1 (MQ=255)
        gcATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGtt                                                                                                                                               <  2:4507247/150‑1 (MQ=255)
         cATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGttt                                                                                                                                              >  2:4094512/1‑150 (MQ=255)
         cATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGttt                                                                                                                                              >  2:1842215/1‑150 (MQ=255)
         cATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGgtt                                                                                                                                              >  2:2486146/1‑150 (MQ=255)
           tCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCTATCTTCGCGTACTCTTCCGATGGCGTTTTg                                                                                                                                            <  1:2653605/150‑1 (MQ=255)
             aGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGAt                                                                                                                                          <  1:4347627/150‑1 (MQ=255)
              gAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCTATGGCGTTTTGAtt                                                                                                                                         <  2:2180500/150‑1 (MQ=255)
                  gCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCt                                                                                                                                     >  1:1558614/1‑150 (MQ=255)
                  gCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCt                                                                                                                                     <  2:1686124/150‑1 (MQ=255)
                  gCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCt                                                                                                                                     >  1:1558771/1‑150 (MQ=255)
                   cGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTg                                                                                                                                    >  1:1999358/1‑150 (MQ=255)
                    gACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGt                                                                                                                                   >  2:50524/1‑150 (MQ=255)
                    gACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGt                                                                                                                                   >  1:1683203/1‑150 (MQ=255)
                      cTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCTATGGCGTTTTGATTGCCTGttt                                                                                                                                 >  2:1318936/1‑150 (MQ=255)
                       tCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTa                                                                                                                                >  2:4435532/1‑150 (MQ=255)
                       tCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTa                                                                                                                                <  2:2425048/150‑1 (MQ=255)
                        cgccgcTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCAATGGCGTTTTGATTGCCTGTTTaa                                                                                                                               >  2:2330502/1‑150 (MQ=255)
                            gcTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGcc                                                                                                                           >  1:3276903/1‑150 (MQ=255)
                              tGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCg                                                                                                                         <  2:2312679/150‑1 (MQ=255)
                                cGGGTAACGCGTTTTGGACTAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCt                                                                                                                       >  1:3881091/1‑150 (MQ=255)
                                cGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCt                                                                                                                       >  2:2633971/1‑150 (MQ=255)
                                  ggTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTAc                                                                                                                     <  1:98345/150‑1 (MQ=255)
                                  ggTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTAc                                                                                                                     <  1:3701470/150‑1 (MQ=255)
                                  ggTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTAc                                                                                                                     <  1:2918369/150‑1 (MQ=255)
                                  ggTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTAc                                                                                                                     >  2:3816496/1‑150 (MQ=255)
                                   gTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACg                                                                                                                    <  1:3868926/150‑1 (MQ=255)
                                   gTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACg                                                                                                                    <  1:4092567/150‑1 (MQ=255)
                                       cgcgTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACt                                                                                                                >  2:2915722/1‑150 (MQ=255)
                                       cgcgTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTGAAGGCCCGCTACGTACt                                                                                                                >  2:2916324/1‑150 (MQ=255)
                                         cgTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCg                                                                                                              <  2:1121963/150‑1 (MQ=255)
                                            tttGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTaa                                                                                                           >  2:719410/1‑150 (MQ=255)
                                            tttGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTaa                                                                                                           <  2:537990/150‑1 (MQ=255)
                                                gACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGAt                                                                                                       <  2:4035246/150‑1 (MQ=255)
                                                gACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGAt                                                                                                       >  1:3285338/1‑150 (MQ=255)
                                                  cGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCg                                                                                                     <  1:7979/150‑1 (MQ=255)
                                                   gAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGt                                                                                                    >  1:4110877/1‑150 (MQ=255)
                                                    aaTCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGtt                                                                                                   >  1:546995/1‑150 (MQ=255)
                                                          cgcgTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTGACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGt                                                                                             <  2:3138342/150‑1 (MQ=255)
                                                           gcgTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTa                                                                                            <  1:4253739/150‑1 (MQ=255)
                                                              tACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATa                                                                                         >  1:2135559/1‑150 (MQ=255)
                                                                 aGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCTATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCa                                                                                      >  1:486800/1‑150 (MQ=255)
                                                                 aGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCa                                                                                      <  2:2272531/150‑1 (MQ=255)
                                                                   ttCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCTATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAg                                                                                    >  2:607937/1‑150 (MQ=255)
                                                                      gTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGAtt                                                                                 >  1:3785170/1‑150 (MQ=255)
                                                                      gTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGAtt                                                                                 >  2:2359542/1‑150 (MQ=255)
                                                                      gTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGAtt                                                                                 >  2:3240827/1‑150 (MQ=255)
                                                                      gTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTAGTAAAGATCGTTGAAGGTAATACCAAGAtt                                                                                 >  1:673072/1‑150 (MQ=255)
                                                                       ttttCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTg                                                                                <  1:104274/150‑1 (MQ=255)
                                                                       ttttCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTg                                                                                >  2:2939873/1‑150 (MQ=255)
                                                                        tttCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGc                                                                               >  1:2232622/1‑150 (MQ=255)
                                                                         ttCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCt                                                                              >  2:2594320/1‑150 (MQ=255)
                                                                         ttCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCt                                                                              >  2:2094971/1‑150 (MQ=255)
                                                                            aaTCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCTATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTg                                                                           >  2:396522/1‑150 (MQ=255)
                                                                            aaTCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTg                                                                           <  2:3038960/150‑1 (MQ=255)
                                                                             aTCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGc                                                                          >  2:4153122/1‑150 (MQ=255)
                                                                              tCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCg                                                                         <  2:3402597/150‑1 (MQ=255)
                                                                                  cAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGAttt                                                                     <  1:2759659/150‑1 (MQ=255)
                                                                                  cAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGAttt                                                                     <  1:3423653/150‑1 (MQ=255)
                                                                                   aaCACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTa                                                                    >  1:2301443/1‑150 (MQ=255)
                                                                                     cacGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTAtt                                                                  <  2:188122/150‑1 (MQ=255)
                                                                                      acGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTg                                                                 <  2:1012281/150‑1 (MQ=255)
                                                                                        gTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGt                                                               >  1:33487/1‑150 (MQ=255)
                                                                                         ttGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATTGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGta                                                              <  2:766384/150‑1 (MQ=255)
                                                                                           gCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGtata                                                            <  1:1817305/150‑1 (MQ=255)
                                                                                            cTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAg                                                           <  2:2128393/150‑1 (MQ=255)
                                                                                            cTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAg                                                           >  1:3956704/1‑150 (MQ=255)
                                                                                             tGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGc                                                          >  2:3749659/1‑150 (MQ=255)
                                                                                              gTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGcc                                                         <  1:1358918/150‑1 (MQ=255)
                                                                                              gTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGcc                                                         >  1:3833255/1‑150 (MQ=255)
                                                                                               ttGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCg                                                        <  1:4127450/150‑1 (MQ=255)
                                                                                               ttGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCg                                                        >  1:3310488/1‑150 (MQ=255)
                                                                                                 gATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCgag                                                      <  1:2359542/150‑1 (MQ=255)
                                                                                                 gATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCgag                                                      >  2:4257275/1‑150 (MQ=255)
                                                                                                 gATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCgag                                                      <  1:3240807/150‑1 (MQ=255)
                                                                                                  aTTTGCAGCCTCTTACCGTCTTTCTCAAGACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCgaga                                                     <  2:3333498/150‑1 (MQ=255)
                                                                                                     tGCAGCCTCTTACCGTCGTTCTCACTACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCg                                                  <  1:3173949/150‑1 (MQ=255)
                                                                                                        aGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTc                                               <  2:1258596/150‑1 (MQ=255)
                                                                                                           ctctTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCaaa                                            >  1:3054436/1‑150 (MQ=255)
                                                                                                           ctctTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCaaa                                            >  1:1487206/1‑150 (MQ=255)
                                                                                                                       ttCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTc                                <  2:117228/150‑1 (MQ=255)
                                                                                                                         ctaaaTACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGc                              >  2:3524142/4‑150 (MQ=255)
                                                                                                                               aCCAATCTTCGCGTACTCTTCCTATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACgg                        >  1:1024788/1‑150 (MQ=255)
                                                                                                                               aCCAATCTTCGCGTACTCTTCCTATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACgg                        >  1:1025648/1‑150 (MQ=255)
                                                                                                                               aCCAATCTTCGCGTACTCTTCCTATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACgg                        >  1:869266/1‑150 (MQ=255)
                                                                                                                               aCCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACgg                        >  1:908105/1‑150 (MQ=255)
                                                                                                                               aCCAATCTTCGCGAACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTNCTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGACGAGATCGCTCAAACGAAGAGAGGTCGCATACgg                        >  1:907197/1‑150 (MQ=255)
                                                                                                                                 cAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCa                      >  2:722453/1‑150 (MQ=255)
                                                                                                                                  aaTCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAg                     >  2:681540/1‑150 (MQ=255)
                                                                                                                                   aTCTTCGCGTACTCTTCCTATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAgg                    <  2:1634709/150‑1 (MQ=255)
                                                                                                                                    tCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGt                   <  2:1740264/150‑1 (MQ=255)
                                                                                                                                     cTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTa                  >  2:386791/1‑150 (MQ=255)
                                                                                                                                     cTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTa                  <  1:2158047/150‑1 (MQ=255)
                                                                                                                                         gcgTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAatac              <  1:4059890/150‑1 (MQ=255)
                                                                                                                                                ctTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGc       >  2:2892010/1‑150 (MQ=255)
                                                                                                                                                 ttCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCa      <  2:1628136/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGATTGCTTTGCGATTTATTGGTATAGCCGAGATCGCTCAAACGAAGAGAGGTCGCATACGGCAGGTAATACATACCGCACTCG  >  CP009273/2808838‑2809136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A