Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F202 I0 R1	1397	275.7	9089454	95.2%	8653160	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,270,316	G→T	57.7%	E137* (GAA→TAA)	yjcE →	putative cation/proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,270,316	0	G	T	57.7%	38.8 / inf	232	E137* (GAA→TAA)	yjcE	putative cation/proton antiporter
Reads supporting (aligned to +/- strand): ref base G (46/52); new base T (66/68); total (112/120)
Fisher's exact test for biased strand distribution p-value = 7.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

Only 100 of 234 total aligned reads displayed.

CCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATG  >  CP009273/4270168‑4270465

ccTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGa                                                                                                                                                      >  2:1811457/1‑150 (MQ=255)

    gcgctggcgctgGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAggg                                                                                                                                                  <  2:3476948/150‑1 (MQ=255)

    gcgctggcgctgGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAggg                                                                                                                                                  <  2:1630687/150‑1 (MQ=255)

    gcgctggcgctgGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAggg                                                                                                                                                  >  1:225067/1‑150 (MQ=255)

        tggcgctggTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCa                                                                                                                                              >  1:4544704/1‑150 (MQ=255)

         ggcgctggTGGTGGTTACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCAt                                                                                                                                             <  2:1179014/150‑1 (MQ=255)

         ggcgctggTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGCTTGTTGGCTAAGGGCGCAt                                                                                                                                             >  2:2636381/1‑150 (MQ=255)

            gctggTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATccc                                                                                                                                          <  1:2270540/150‑1 (MQ=255)

            gctggTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATccc                                                                                                                                          >  2:2302935/1‑150 (MQ=255)

            gctggTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATccc                                                                                                                                          <  1:1365918/150‑1 (MQ=255)

               ggtggtggtCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGaa                                                                                                                                       <  2:897843/150‑1 (MQ=255)

                gtggtggtCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGaaa                                                                                                                                      <  1:1000953/150‑1 (MQ=255)

                 tggtggtCACCGTGGTCGGCATTGGCTTCCTTATTTAGTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGaaaa                                                                                                                                     >  2:3229143/1‑150 (MQ=255)

                   gtggtAACCGGGGTCGGCATTGGCTTCCTTATTTACTGGTTTCTTCCGTTCATTCCGCTGATCCCGGCCTTTGCGCTGGCGTCTGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGaaaaaa                                                                                                                                   <  2:2238601/150‑1 (MQ=255)

                    tggtCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGaaaaaaa                                                                                                                                  <  1:4194883/150‑1 (MQ=255)

                     ggtCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAAt                                                                                                                                 <  1:3195468/150‑1 (MQ=255)

                            gTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGc                                                                                                                          >  1:3098941/1‑150 (MQ=255)

                            gTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGc                                                                                                                          <  2:1119600/150‑1 (MQ=255)

                              ggTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCAt                                                                                                                        <  1:1188733/150‑1 (MQ=255)

                               gTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCAtt                                                                                                                       <  1:2781706/150‑1 (MQ=255)

                                  ggCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTg                                                                                                                    >  1:1169756/1‑150 (MQ=255)

                                  ggCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTg                                                                                                                    >  1:1171274/1‑150 (MQ=255)

                                  ggCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTg                                                                                                                    <  1:53794/150‑1 (MQ=255)

                                       tGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAggg                                                                                                               <  1:2495721/150‑1 (MQ=255)

                                        ggCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGc                                                                                                              >  1:3309024/1‑150 (MQ=255)

                                             ccTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGc                                                                                                         >  2:3052255/1‑150 (MQ=255)

                                              cTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCg                                                                                                        <  2:1346530/150‑1 (MQ=255)

                                               ttATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGt                                                                                                       <  1:691700/150‑1 (MQ=255)

                                                tATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGtt                                                                                                      <  2:3959504/150‑1 (MQ=255)

                                                tATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGtt                                                                                                      >  1:1286964/1‑150 (MQ=255)

                                                   ttACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTtgat                                                                                                   >  1:4217352/1‑150 (MQ=255)

                                                   ttACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTtgat                                                                                                   >  2:1395346/1‑150 (MQ=255)

                                                    tACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCCTGGGCATTTTGCAGGGCGAAGCGTtgctg                                                                                                  >  2:2914131/1‑150 (MQ=255)

                                                      cagggTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGatgaa                                                                                                >  1:3447822/3‑150 (MQ=255)

                                                      cTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGatgaa                                                                                                <  1:1445163/150‑1 (MQ=255)

                                                      cTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGatgaa                                                                                                >  1:3447594/1‑150 (MQ=255)

                                                          gtggtgCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGAc                                                                                            <  2:4225491/150‑1 (MQ=255)

                                                          gtggtgCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGAc                                                                                            >  1:847509/1‑150 (MQ=255)

                                                           tggtgCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACg                                                                                           >  2:943518/1‑150 (MQ=255)

                                                            ggtgCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTACACCGACCGATGCTGTGGCGCTCTCAGGGATTGTTGGGTCAGGGCTCATCCCGAAAAATATCATAGGCATTTTGCAGGGCGAAGCGGTGATGAATGACGc                                                                                          >  2:1587977/1‑150 (MQ=255)

                                                               gCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTc                                                                                       >  2:1723679/1‑150 (MQ=255)

                                                               gCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTc                                                                                       >  1:896196/1‑150 (MQ=255)

                                                               gCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTc                                                                                       >  1:896209/1‑150 (MQ=255)

                                                                ccGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTcc                                                                                      >  1:3002846/1‑150 (MQ=255)

                                                                 cGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCg                                                                                     >  1:3878519/1‑150 (MQ=255)

                                                                   ggCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGt                                                                                   >  1:1864033/1‑150 (MQ=255)

                                                                     cATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCt                                                                                 <  2:2791188/150‑1 (MQ=255)

                                                                      aTTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGACTGACGCCTCCGGTCTg                                                                                >  2:3106731/1‑150 (MQ=255)

                                                                      aTTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTg                                                                                >  2:3107150/1‑150 (MQ=255)

                                                                       ttCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTgg                                                                               >  1:556889/1‑150 (MQ=255)

                                                                         ccGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGgtg                                                                             <  1:136596/150‑1 (MQ=255)

                                                                          cGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGgtgt                                                                            >  1:2012915/1‑150 (MQ=255)

                                                                           gCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTc                                                                           <  1:4059695/150‑1 (MQ=255)

                                                                           gCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTc                                                                           <  1:2868259/150‑1 (MQ=255)

                                                                           gCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTc                                                                           >  1:1390163/1‑150 (MQ=255)

                                                                              gATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGtt                                                                        >  2:1997138/1‑150 (MQ=255)

                                                                                tCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGa                                                                      >  1:2841990/1‑150 (MQ=255)

                                                                                tCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGa                                                                      >  2:1816186/1‑150 (MQ=255)

                                                                                 cccTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGaa                                                                     <  1:4476014/150‑1 (MQ=255)

                                                                                  ccTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAg                                                                    <  1:1269256/150‑1 (MQ=255)

                                                                                   cTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGt                                                                   <  2:1050991/150‑1 (MQ=255)

                                                                                      ccTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTg                                                                <  1:1719891/150‑1 (MQ=255)

                                                                                      ccTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTg                                                                <  1:1575130/150‑1 (MQ=255)

                                                                                      ccTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTg                                                                <  2:2592772/150‑1 (MQ=255)

                                                                                       cTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGc                                                               >  1:3992212/1‑150 (MQ=255)

                                                                                            cgcTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAg                                                          <  2:2655329/150‑1 (MQ=255)

                                                                                             gcTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGc                                                         >  2:2203290/1‑150 (MQ=255)

                                                                                              cTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCa                                                        <  2:1408804/150‑1 (MQ=255)

                                                                                                 gcggcgGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTg                                                     <  1:2519867/150‑1 (MQ=255)

                                                                                                  cggcggTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTgg                                                    <  2:344288/150‑1 (MQ=255)

                                                                                                     cggTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGACTGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGa                                                 <  1:1135172/150‑1 (MQ=255)

                                                                                                          cTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATgggg                                            >  2:3986670/1‑150 (MQ=255)

                                                                                                               tcCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACgat                                       <  1:679267/150‑1 (MQ=255)

                                                                                                               tcCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACgat                                       >  1:3315212/1‑150 (MQ=255)

                                                                                                                    ccgaTGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCt                                  <  2:4203250/150‑1 (MQ=255)

                                                                                                                    ccgaTGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCt                                  <  2:2422647/150‑1 (MQ=255)

                                                                                                                     cgaTGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCtt                                 >  2:4535902/1‑150 (MQ=255)

                                                                                                                     cgaTGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCtt                                 >  1:3118505/1‑150 (MQ=255)

                                                                                                                        tGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCAc                              <  1:1062438/150‑1 (MQ=255)

                                                                                                                           tgtgGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGt                           >  2:2043185/1‑150 (MQ=255)

                                                                                                                           tgtgGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGt                           >  2:426516/1‑150 (MQ=255)

                                                                                                                               gcgcTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTcggc                       >  1:3975403/1‑150 (MQ=255)

                                                                                                                                  ctctCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGt                    >  2:46013/1‑150 (MQ=255)

                                                                                                                                    ctcCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGc                  <  1:3397956/150‑1 (MQ=255)

                                                                                                                                    ctcCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGc                  <  1:1120345/150‑1 (MQ=255)

                                                                                                                                      ccGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGa                >  1:2136183/1‑150 (MQ=255)

                                                                                                                                      ccGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGa                >  2:3596345/1‑150 (MQ=255)

                                                                                                                                       cGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGAc               >  2:1450748/1‑150 (MQ=255)

                                                                                                                                       cGGGATTGTTGGAGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGATGATGAATGACGCCTCCGGTCTGGTGTCGTTGATGTTTGCCGTAGCAGTGGCGATGGGGTCGATGATCTTCACCGTCGGCGGTGCGAc               <  2:1516922/150‑1 (MQ=255)

                                                                                                                                        gggATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACg              <  1:2722499/150‑1 (MQ=255)

                                                                                                                                        gggATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACg              >  1:4152074/1‑150 (MQ=255)

                                                                                                                                          gATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGt            >  2:2925772/1‑150 (MQ=255)

                                                                                                                                           aTTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTc           <  1:1450619/150‑1 (MQ=255)

                                                                                                                                                tgGCTAATGGCGCATCCCGAAAAAAATCATGGGCATATTGCAGGGCGAAGAGTAGATGAATGACGCCTCCTGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGTTGCGACGGTCGAAtt      >  2:2109382/1‑150 (MQ=255)

                                                                                                                                                tgGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAAtt      >  2:553867/1‑150 (MQ=255)

                                                                                                                                                tgGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAAtt      <  2:3870543/150‑1 (MQ=255)

                                                                                                                                                tgGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAAtt      >  2:2501975/1‑150 (MQ=255)

                                                                                                                                                tgGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAAtt      >  2:2109428/1‑150 (MQ=255)

                                                                                                                                                  gCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTa    <  2:1473301/150‑1 (MQ=255)

                                                                                                                                                   cGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTAt   <  1:2467623/150‑1 (MQ=255)

CCTCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATG  >  CP009273/4270168‑4270465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATG  >  CP009273/4270170‑4270465

TCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAG                                                                                                                                                    <  SRR19973076.866754/150‑1 (MQ=60)

 CGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGG                                                                                                                                                   <  SRR19973076.2128063/150‑1 (MQ=60)

  GCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGG                                                                                                                                                  >  SRR19973076.225454/1‑150 (MQ=60)

    GCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCG                                                                                                                                                <  SRR19973076.3021574/150‑1 (MQ=60)

      TGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCA                                                                                                                                              >  SRR19973076.4551446/1‑150 (MQ=60)

          GCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCC                                                                                                                                          <  SRR19973076.2273795/150‑1 (MQ=60)

          GCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCC                                                                                                                                          <  SRR19973076.2273615/150‑1 (MQ=60)

          GCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCC                                                                                                                                          <  SRR19973076.1367960/150‑1 (MQ=60)

          GCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCC                                                                                                                                          <  SRR19973076.1367927/150‑1 (MQ=60)

              GTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAA                                                                                                                                      <  SRR19973076.1002439/150‑1 (MQ=60)

               TGGTGGTCTCCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCAGTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAA                                                                                                                                     <  SRR19973076.2525907/150‑1 (MQ=60)

                  TGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAA                                                                                                                                  <  SRR19973076.4201078/150‑1 (MQ=60)

                  TGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAA                                                                                                                                  <  SRR19973076.4201105/150‑1 (MQ=60)

                   GGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAAT                                                                                                                                 <  SRR19973076.3200107/150‑1 (MQ=60)

                   GGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAAT                                                                                                                                 <  SRR19973076.3200094/150‑1 (MQ=60)

                      CACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCAT                                                                                                                              <  SRR19973076.446691/150‑1 (MQ=60)

                        CCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGG                                                                                                                            <  SRR19973076.1304955/150‑1 (MQ=60)

                          GTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGC                                                                                                                          >  SRR19973076.3103434/1‑150 (MQ=60)

                            GGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCAT                                                                                                                        <  SRR19973076.1190502/150‑1 (MQ=60)

                             GTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATT                                                                                                                       <  SRR19973076.2785749/150‑1 (MQ=60)

                                GGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTG                                                                                                                    >  SRR19973076.1173011/1‑150 (MQ=60)

                                GGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTG                                                                                                                    >  SRR19973076.1171489/1‑150 (MQ=60)

                                GGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTG                                                                                                                    >  SRR19973076.1171483/1‑150 (MQ=60)

                                GGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTG                                                                                                                    <  SRR19973076.53912/150‑1 (MQ=60)

                                GGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTG                                                                                                                    <  SRR19973076.53893/150‑1 (MQ=60)

                                GGCATTGGCTTCCTTATGTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGAGTGTTGGCGACGGGCGCATCCCGAAAAAAATCATGGGCATTTTG                                                                                                                    >  SRR19973076.1171506/1‑150 (MQ=60)

                                     TGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGG                                                                                                               <  SRR19973076.4160453/150‑1 (MQ=60)

                                     TGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGG                                                                                                               <  SRR19973076.2499342/150‑1 (MQ=60)

                                     TGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGG                                                                                                               <  SRR19973076.2499347/150‑1 (MQ=60)

                                     TGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGG                                                                                                               <  SRR19973076.4160419/150‑1 (MQ=60)

                                      GGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGC                                                                                                              >  SRR19973076.3314481/1‑150 (MQ=60)

                                      GGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGC                                                                                                              >  SRR19973076.3313834/1‑150 (MQ=60)

                                         ttccttatttactgggtggtgccgggcattcttttgtttgttttttttgttgttggttttgttttttttttgtttttTGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAA                                                                                                           <  SRR19973076.2867537/73‑1 (MQ=60)

                                             TTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGT                                                                                                       <  SRR19973076.692738/150‑1 (MQ=60)

                                             TTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGT                                                                                                       <  SRR19973076.692692/150‑1 (MQ=60)

                                              TATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTT                                                                                                      >  SRR19973076.1288869/1‑150 (MQ=60)

                                              TATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTT                                                                                                      >  SRR19973076.1288827/1‑150 (MQ=60)

                                               ATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTG                                                                                                     <  SRR19973076.1449192/150‑1 (MQ=60)

                                               ATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTG                                                                                                     <  SRR19973076.1447179/150‑1 (MQ=60)

                                                 TTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGAT                                                                                                   >  SRR19973076.222284/1‑150 (MQ=60)

                                                 TTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGAT                                                                                                   >  SRR19973076.4223578/1‑150 (MQ=60)

                                                    CTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAA                                                                                                <  SRR19973076.1447281/150‑1 (MQ=60)

                                                    CTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAA                                                                                                >  SRR19973076.3452581/1‑150 (MQ=60)

                                                    CTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAA                                                                                                >  SRR19973076.3452667/1‑150 (MQ=60)

                                                    CTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAA                                                                                                >  SRR19973076.3452787/1‑150 (MQ=60)

                                                    CTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAA                                                                                                <  SRR19973076.45052/150‑1 (MQ=60)

                                                    CAGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAA                                                                                                >  SRR19973076.3452809/1‑150 (MQ=60)

                                                     TGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAAT                                                                                               >  SRR19973076.4004034/1‑150 (MQ=60)

                                                        GTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGAC                                                                                            >  SRR19973076.848751/1‑150 (MQ=60)

                                                             GCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTC                                                                                       >  SRR19973076.897540/1‑150 (MQ=60)

                                                             GCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTC                                                                                       >  SRR19973076.897527/1‑150 (MQ=60)

                                                             GCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTC                                                                                       >  SRR19973076.897528/1‑150 (MQ=60)

                                                             GCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTC                                                                                       <  SRR19973076.1056720/150‑1 (MQ=60)

                                                              CCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCC                                                                                      >  SRR19973076.3007215/1‑150 (MQ=60)

                                                              CCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCC                                                                                      >  SRR19973076.3004220/1‑150 (MQ=60)

                                                               CGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCG                                                                                     >  SRR19973076.3884243/1‑150 (MQ=60)

                                                               CGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCG                                                                                     >  SRR19973076.3884252/1‑150 (MQ=60)

                                                               CGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCG                                                                                     >  SRR19973076.1359089/1‑150 (MQ=60)

                                                                 GGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGT                                                                                   >  SRR19973076.1866727/1‑150 (MQ=60)

                                                                 GGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGT                                                                                   >  SRR19973076.3629210/1‑150 (MQ=60)

                                                                   CATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCT                                                                                 <  SRR19973076.1091543/150‑1 (MQ=60)

                                                                    ATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTG                                                                                >  SRR19973076.706636/1‑150 (MQ=60)

                                                                     TTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGG                                                                               >  SRR19973076.557773/1‑150 (MQ=60)

                                                                       CCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTG                                                                             <  SRR19973076.3409506/150‑1 (MQ=60)

                                                                       CCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTG                                                                             <  SRR19973076.136836/150‑1 (MQ=60)

                                                                        CGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGT                                                                            >  SRR19973076.2015732/1‑150 (MQ=60)

                                                                        CGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGT                                                                            >  SRR19973076.2015857/1‑150 (MQ=60)

                                                                         GCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTC                                                                           <  SRR19973076.4065693/150‑1 (MQ=60)

                                                                         GCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTC                                                                           <  SRR19973076.949621/150‑1 (MQ=60)

                                                                         GCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTC                                                                           <  SRR19973076.2872408/150‑1 (MQ=60)

                                                                         GCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTC                                                                           <  SRR19973076.2872368/150‑1 (MQ=60)

                                                                         GCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTC                                                                           <  SRR19973076.2872318/150‑1 (MQ=60)

                                                                         GCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTC                                                                           <  SRR19973076.1189888/150‑1 (MQ=60)

                                                                         GCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTC                                                                           >  SRR19973076.1392269/1‑150 (MQ=60)

                                                                         GCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTC                                                                           >  SRR19973076.1392214/1‑150 (MQ=60)

                                                                         GCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTC                                                                           >  SRR19973076.1391483/1‑150 (MQ=60)

                                                                              TCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGA                                                                      >  SRR19973076.2846103/1‑150 (MQ=60)

                                                                              TCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGA                                                                      >  SRR19973076.2844382/1‑150 (MQ=60)

                                                                               CCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAA                                                                     <  SRR19973076.4482677/150‑1 (MQ=60)

                                                                               CCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAA                                                                     <  SRR19973076.4482681/150‑1 (MQ=60)

                                                                                CCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAG                                                                    <  SRR19973076.1522907/150‑1 (MQ=60)

                                                                                CCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAG                                                                    <  SRR19973076.1271135/150‑1 (MQ=60)

                                                                                   GCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTT                                                                 >  SRR19973076.336316/1‑150 (MQ=60)

                                                                                    CCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTG                                                                <  SRR19973076.1722389/150‑1 (MQ=60)

                                                                                    CCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTG                                                                <  SRR19973076.1577444/150‑1 (MQ=60)

                                                                                     CTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGC                                                               >  SRR19973076.3998060/1‑150 (MQ=60)

                                                                                     CTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGC                                                               >  SRR19973076.3998100/1‑150 (MQ=60)

                                                                                        TGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGT                                                            >  SRR19973076.1202592/1‑150 (MQ=60)

                                                                                               GCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTG                                                     <  SRR19973076.2523534/150‑1 (MQ=60)

                                                                                               GCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTG                                                     <  SRR19973076.2517496/150‑1 (MQ=60)

                                                                                                   CGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGACTGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGA                                                 <  SRR19973076.1136844/150‑1 (MQ=60)

                                                                                                   CGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGA                                                 <  SRR19973076.1136836/150‑1 (MQ=60)

                                                                                                   CGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGA                                                 <  SRR19973076.1136225/150‑1 (MQ=60)

                                                                                                           TCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACG                                         <  SRR19973076.738876/150‑1 (MQ=60)

                                                                                                             TCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGAT                                       <  SRR19973076.680285/150‑1 (MQ=60)

                                                                                                             TCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGAT                                       >  SRR19973076.3106455/1‑150 (MQ=60)

                                                                                                             TCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGAT                                       >  SRR19973076.3320027/1‑150 (MQ=60)

                                                                                                             TCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGAT                                       >  SRR19973076.3320021/1‑150 (MQ=60)

                                                                                                                   CGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTT                                 >  SRR19973076.3123035/1‑150 (MQ=60)

                                                                                                                   CGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTT                                 >  SRR19973076.3123029/1‑150 (MQ=60)

                                                                                                                   CGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTT                                 >  SRR19973076.3123006/1‑150 (MQ=60)

                                                                                                                      TGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCAC                              <  SRR19973076.1064012/150‑1 (MQ=60)

                                                                                                                            GGCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGG                        >  SRR19973076.913475/1‑150 (MQ=60)

                                                                                                                             GCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGC                       >  SRR19973076.3981254/1‑150 (MQ=60)

                                                                                                                             GCGCTCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGC                       >  SRR19973076.3981316/1‑150 (MQ=60)

                                                                                                                             GCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGC                       <  SRR19973076.617407/150‑1 (MQ=60)

                                                                                                                               GCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGG                     <  SRR19973076.4113718/150‑1 (MQ=60)

                                                                                                                                 TCTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTG                   <  SRR19973076.163535/150‑1 (MQ=60)

                                                                                                                                  CTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGC                  <  SRR19973076.3402878/150‑1 (MQ=60)

                                                                                                                                  CTCCGGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGC                  <  SRR19973076.1121998/150‑1 (MQ=60)

                                                                                                                                    CCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGA                >  SRR19973076.2139283/1‑150 (MQ=60)

                                                                                                                                    CCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGA                >  SRR19973076.2139255/1‑150 (MQ=60)

                                                                                                                                      GGGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACG              <  SRR19973076.2726463/150‑1 (MQ=60)

                                                                                                                                      GGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACG              >  SRR19973076.4158204/1‑150 (MQ=60)

                                                                                                                                       GGATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGG             <  SRR19973076.2640223/150‑1 (MQ=60)

                                                                                                                                         ATTGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTC           <  SRR19973076.1452747/150‑1 (MQ=60)

                                                                                                                                           TGTTGGCTAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGA         <  SRR19973076.547502/150‑1 (MQ=60)

                                                                                                                                           TGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGA         <  SRR19973076.2748687/150‑1 (MQ=60)

                                                                                                                                            GTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAA        <  SRR19973076.163542/150‑1 (MQ=60)

                                                                                                                                              TGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATT      <  SRR19973076.1814065/150‑1 (MQ=60)

                                                                                                                                                 CGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTAT   <  SRR19973076.2471206/150‑1 (MQ=60)

                                                                                                                                                 CGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTAT   <  SRR19973076.2471201/150‑1 (MQ=60)

                                                                                                                                                 CGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTAT   <  SRR19973076.2470051/150‑1 (MQ=60)

                                                                                                                                                  GAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATG  <  SRR19973076.11900/150‑1 (MQ=60)

                                                                                                                                                  GAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATG  <  SRR19973076.622149/150‑1 (MQ=60)

TCGCGCTGGCGCTGGTGGTGGTCACCGTGGTCGGCATTGGCTTCCTTATTTACTGGGTGGTGCCGGGCATTCCGCTGATCCCTGCCTTTGCGCTGGCGGCGGTGCTTTCTCCGACCGATGCTGTGGCGCTCTCCGGGATTGTTGGCGAAGGGCGCATCCCGAAAAAAATCATGGGCATTTTGCAGGGCGAAGCGTTGATGAATGACGCCTCCGGTCTGGTGTCGTTGAAGTTTGCCGTAGCAGTGGCGATGGGGACGATGATCTTCACCGTCGGCGGTGCGACGGTCGAATTTATG  >  CP009273/4270170‑4270465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`