Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 480,820 C→T 90.1% D86N (GAC→AAC)  acrA ← multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273480,8200CT90.1% 799.4 / 62.0 253D86N (GAC→AAC) acrAmultidrug efflux system
Reads supporting (aligned to +/- strand):  ref base C (14/11);  new base T (112/116);  total (126/127)
Fisher's exact test for biased strand distribution p-value = 5.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 253 total aligned reads displayed.

ACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCA  >  CP009273/480674‑480966
                                                                                                                                                  |                                                                                                                                                  
aCCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCt                                                                                                                                                 <  2:2919394/150‑1 (MQ=255)
   gAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTAcc                                                                                                                                              >  1:482078/1‑150 (MQ=255)
    aGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACct                                                                                                                                             >  2:4525020/1‑150 (MQ=255)
       aGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACcttct                                                                                                                                          <  1:3765001/150‑1 (MQ=255)
        gTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACcttctt                                                                                                                                         <  2:4098426/150‑1 (MQ=255)
          ttCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTg                                                                                                                                       >  1:1052370/1‑150 (MQ=255)
          ttCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTg                                                                                                                                       >  1:1052761/1‑150 (MQ=255)
          ttCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTg                                                                                                                                       >  1:437673/1‑150 (MQ=255)
                 aaCGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTAc                                                                                                                                >  2:1848096/1‑150 (MQ=255)
                 aaCGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTAc                                                                                                                                <  1:3133975/150‑1 (MQ=255)
                  aCGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACg                                                                                                                               >  2:83968/1‑150 (MQ=255)
                    gATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCt                                                                                                                             >  2:1017106/1‑150 (MQ=255)
                     aTTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCtt                                                                                                                            <  1:1233321/150‑1 (MQ=255)
                           cGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGAt                                                                                                                      >  1:3462662/1‑150 (MQ=255)
                            gTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATa                                                                                                                     >  1:1813789/1‑150 (MQ=255)
                            gTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATa                                                                                                                     >  1:562261/1‑150 (MQ=255)
                            gTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATa                                                                                                                     >  1:2825336/1‑150 (MQ=255)
                             tCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATaa                                                                                                                    <  2:3112418/150‑1 (MQ=255)
                              cAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAAt                                                                                                                   >  1:4102708/1‑150 (MQ=255)
                              cAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAAt                                                                                                                   >  1:2486277/1‑150 (MQ=255)
                              cAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAAt                                                                                                                   >  1:2649168/1‑150 (MQ=255)
                               aaTTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATc                                                                                                                  <  2:4450853/150‑1 (MQ=255)
                                aTTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATcc                                                                                                                 <  2:390200/150‑1 (MQ=255)
                                     gcgATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTa                                                                                                            <  2:2579425/150‑1 (MQ=255)
                                      cgATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTaa                                                                                                           <  1:1595164/150‑1 (MQ=255)
                                          atTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTg                                                                                                       <  2:2016601/150‑1 (MQ=255)
                                          atTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTg                                                                                                       <  1:4474153/150‑1 (MQ=255)
                                            tGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCAGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAg                                                                                                     >  2:303989/1‑150 (MQ=255)
                                             ggCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAgg                                                                                                    >  1:4056911/1‑150 (MQ=255)
                                              gCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGa                                                                                                   <  1:1986875/150‑1 (MQ=255)
                                                tGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACg                                                                                                 >  1:1199396/1‑150 (MQ=255)
                                                      cTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTc                                                                                           <  1:2396307/150‑1 (MQ=255)
                                                       tGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCt                                                                                          >  2:2400788/1‑150 (MQ=255)
                                                          gCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCg                                                                                       <  2:1570569/150‑1 (MQ=255)
                                                           cTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGa                                                                                      >  2:1288251/1‑150 (MQ=255)
                                                             ttCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATc                                                                                    >  1:1342841/1‑150 (MQ=255)
                                                              tCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATcc                                                                                   >  1:890647/1‑150 (MQ=255)
                                                               cGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg                                                                                  <  1:2365224/150‑1 (MQ=255)
                                                                gCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTTATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg                                                                                 >  1:3087696/1‑150 (MQ=255)
                                                                 ccATATCCCCTTTCGCACTGTCGTATGTCGGCCGCTTGGTAGCATGAACAATCTTATAGAGTGATACACCGTCTTCGCTTTTGCTTCCCTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGt                                                                                <  2:4497165/150‑1 (MQ=255)
                                                                    gATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAgg                                                                             >  1:3883496/1‑150 (MQ=255)
                                                                       cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCAc                                                                          >  2:4153519/1‑150 (MQ=255)
                                                                       cACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCCCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGACATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCAc                                                                          >  2:3555762/1‑150 (MQ=255)
                                                                         ccTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                                                                        >  2:3118357/1‑150 (MQ=255)
                                                                          cTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTgg                                                                       <  2:1971338/150‑1 (MQ=255)
                                                                          cTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTgg                                                                       >  2:1139448/1‑150 (MQ=255)
                                                                             tCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGc                                                                    <  2:2940873/150‑1 (MQ=255)
                                                                               gCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGa                                                                  <  2:2526093/150‑1 (MQ=255)
                                                                               gCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGa                                                                  >  1:3346366/1‑150 (MQ=255)
                                                                               gCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTCCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGa                                                                  >  1:822650/1‑150 (MQ=255)
                                                                                cACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAc                                                                 >  1:4359524/1‑150 (MQ=255)
                                                                                  cTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATACTCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAccc                                                               <  2:1165704/150‑1 (MQ=255)
                                                                                  cTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAccc                                                               >  2:1235781/1‑150 (MQ=255)
                                                                                      cGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGaa                                                           <  2:437673/150‑1 (MQ=255)
                                                                                      cGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGaa                                                           <  1:3902458/150‑1 (MQ=255)
                                                                                       gTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAg                                                          <  2:2183920/150‑1 (MQ=255)
                                                                                       gTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAg                                                          >  1:1331554/1‑150 (MQ=255)
                                                                                        tATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGc                                                         <  1:3472596/150‑1 (MQ=255)
                                                                                        tATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGc                                                         >  2:3717384/1‑150 (MQ=255)
                                                                                         aTGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCt                                                        >  2:2325816/1‑150 (MQ=255)
                                                                                         aTGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCt                                                        <  2:3963648/150‑1 (MQ=255)
                                                                                         aTGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCt                                                        >  2:852880/1‑150 (MQ=255)
                                                                                          tGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTc                                                       <  2:244744/150‑1 (MQ=255)
                                                                                           gTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCg                                                      >  2:1365676/1‑150 (MQ=255)
                                                                                            tCGCCTGATAGGTCGCAGGATCAATCTGAGAGAGAGAGACACCTGCGTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCgg                                                     <  2:65491/150‑1 (MQ=255)
                                                                                             cGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGt                                                    <  1:497600/150‑1 (MQ=255)
                                                                                                 tGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg                                                >  2:2760658/1‑150 (MQ=255)
                                                                                                    tAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATc                                             >  1:1532073/1‑150 (MQ=255)
                                                                                                     aGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCt                                            <  2:1637158/150‑1 (MQ=255)
                                                                                                       gTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGc                                          <  2:2250006/150‑1 (MQ=255)
                                                                                                            aGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAgg                                     >  2:648300/1‑150 (MQ=255)
                                                                                                              taTCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGtt                                   >  1:2675171/2‑150 (MQ=255)
                                                                                                               aTCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTc                                  >  1:4226229/1‑150 (MQ=255)
                                                                                                                tCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCa                                 <  1:3029916/150‑1 (MQ=255)
                                                                                                                tCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCa                                 <  1:4169871/150‑1 (MQ=255)
                                                                                                                 cAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAg                                >  1:1032837/1‑150 (MQ=255)
                                                                                                                 cAATCTGATAGAGAGAGACAACTGCATCGATGTTGCTACCTTCTTTGAAATTACGATTCAGGATAATCCCGCTAACTTGAGGACGAACATCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAg                                >  2:4000313/1‑150 (MQ=255)
                                                                                                                  aaTCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGt                               >  2:748799/1‑150 (MQ=255)
                                                                                                                  aaTCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGt                               <  2:1881034/150‑1 (MQ=255)
                                                                                                                  aaTCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGt                               >  2:359222/1‑150 (MQ=255)
                                                                                                                  aaTCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGt                               <  1:3874842/150‑1 (MQ=255)
                                                                                                                    tCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGttt                             >  2:3283537/1‑150 (MQ=255)
                                                                                                                     cTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGtttt                            <  1:1591869/150‑1 (MQ=255)
                                                                                                                      tGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTg                           >  1:3582713/1‑150 (MQ=255)
                                                                                                                      tGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTg                           <  2:1950714/150‑1 (MQ=255)
                                                                                                                      tGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTg                           <  1:3194257/150‑1 (MQ=255)
                                                                                                                         tAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACt                        <  1:1947556/150‑1 (MQ=255)
                                                                                                                         tAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACt                        <  1:1424908/150‑1 (MQ=255)
                                                                                                                         tAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACt                        >  2:1601254/1‑150 (MQ=255)
                                                                                                                          agagagagACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTg                       >  2:2640774/1‑150 (MQ=255)
                                                                                                                            agagagaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGtt                     >  2:3861717/1‑150 (MQ=255)
                                                                                                                            agagagaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGtt                     <  1:2140559/150‑1 (MQ=255)
                                                                                                                             gagagaCACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTta                    <  1:1409587/150‑1 (MQ=255)
                                                                                                                                  acacCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACg               <  1:16248/150‑1 (MQ=255)
                                                                                                                                   cacCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGc              >  1:1029813/1‑150 (MQ=255)
                                                                                                                                       tGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAc          >  1:683584/1‑150 (MQ=255)
                                                                                                                                        gCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAcg         >  2:1437708/1‑150 (MQ=255)
                                                                                                                                          ttCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAcggc       <  2:1199396/150‑1 (MQ=255)
                                                                                                                                            cGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAcggcgg     <  1:2052919/150‑1 (MQ=255)
                                                                                                                                               tGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCa  <  1:2226907/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCA  >  CP009273/480674‑480966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCT  >  CP009273/480671‑480969
                                                                                                                                                     |                                                                                                                                                     
AGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTT                                                                                                                                                       >  SRR19973080.3775299/1‑150 (MQ=60)
 GTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTG                                                                                                                                                      >  SRR19973080.2863715/1‑150 (MQ=60)
 GTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTG                                                                                                                                                      <  SRR19973080.3688863/150‑1 (MQ=60)
    CCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTA                                                                                                                                                   <  SRR19973080.3693155/150‑1 (MQ=60)
      GAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACC                                                                                                                                                 >  SRR19973080.485145/1‑150 (MQ=60)
          AGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCT                                                                                                                                             >  SRR19973080.2956854/1‑150 (MQ=60)
          AGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCT                                                                                                                                             <  SRR19973080.3785507/150‑1 (MQ=60)
             TTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTG                                                                                                                                          >  SRR19973080.440393/1‑150 (MQ=60)
             TTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTG                                                                                                                                          >  SRR19973080.1058553/1‑150 (MQ=60)
             TTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTG                                                                                                                                          >  SRR19973080.1058937/1‑150 (MQ=60)
             TTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTG                                                                                                                                          >  SRR19973080.1058945/1‑150 (MQ=60)
                    AACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTAC                                                                                                                                   <  SRR19973080.3151009/150‑1 (MQ=60)
                    AACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTAC                                                                                                                                   <  SRR19973080.3151228/150‑1 (MQ=60)
                       GATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCT                                                                                                                                <  SRR19973080.523079/150‑1 (MQ=60)
                        ATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTT                                                                                                                               <  SRR19973080.1240794/150‑1 (MQ=60)
                        ATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTT                                                                                                                               <  SRR19973080.1240768/150‑1 (MQ=60)
                        ATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTT                                                                                                                               <  SRR19973080.2219711/150‑1 (MQ=60)
                         TTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTC                                                                                                                              <  SRR19973080.4274480/150‑1 (MQ=60)
                           CACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAG                                                                                                                            >  SRR19973080.1995186/1‑150 (MQ=60)
                           CACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAG                                                                                                                            >  SRR19973080.1995166/1‑150 (MQ=60)
                           CACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGTAATTACGCTTCAG                                                                                                                            >  SRR19973080.127890/1‑150 (MQ=60)
                           CACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAG                                                                                                                            >  SRR19973080.128344/1‑150 (MQ=60)
                              CGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGAT                                                                                                                         >  SRR19973080.3481646/1‑150 (MQ=60)
                              CGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGAT                                                                                                                         <  SRR19973080.160544/150‑1 (MQ=60)
                              CGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGAT                                                                                                                         <  SRR19973080.595469/150‑1 (MQ=60)
                               GTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATA                                                                                                                        >  SRR19973080.1823485/1‑150 (MQ=60)
                               GTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATA                                                                                                                        >  SRR19973080.1824069/1‑150 (MQ=60)
                               GTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATA                                                                                                                        <  SRR19973080.2137982/150‑1 (MQ=60)
                               GTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATA                                                                                                                        >  SRR19973080.2840875/1‑150 (MQ=60)
                               GTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATA                                                                                                                        >  SRR19973080.565796/1‑150 (MQ=60)
                                TCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAA                                                                                                                       <  SRR19973080.2678872/150‑1 (MQ=60)
                                 CAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAAT                                                                                                                      >  SRR19973080.4125008/1‑150 (MQ=60)
                                 CAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAAT                                                                                                                      >  SRR19973080.2663459/1‑150 (MQ=60)
                                 CAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAAT                                                                                                                      >  SRR19973080.2663256/1‑150 (MQ=60)
                                 CAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAAT                                                                                                                      >  SRR19973080.2662888/1‑150 (MQ=60)
                                 CAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAAT                                                                                                                      >  SRR19973080.2500015/1‑150 (MQ=60)
                                  gctcttccgatcTTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATC                                                                                                                     <  SRR19973080.1422576/138‑1 (MQ=60)
                                  AATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATC                                                                                                                     <  SRR19973080.1049175/150‑1 (MQ=60)
                                   ATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCC                                                                                                                    <  SRR19973080.398063/150‑1 (MQ=60)
                                   ATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCC                                                                                                                    <  SRR19973080.398064/150‑1 (MQ=60)
                                    TTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCC                                                                                                                   <  SRR19973080.2384313/150‑1 (MQ=60)
                                      GCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGC                                                                                                                 >  SRR19973080.1350067/1‑150 (MQ=60)
                                       CGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCT                                                                                                                <  SRR19973080.3896926/150‑1 (MQ=60)
                                       CGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCT                                                                                                                <  SRR19973080.110236/150‑1 (MQ=60)
                                         CGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAA                                                                                                              <  SRR19973080.1604485/150‑1 (MQ=60)
                                           ATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCGTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACT                                                                                                            >  SRR19973080.1543485/1‑150 (MQ=60)
                                            TATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTT                                                                                                           <  SRR19973080.1875975/150‑1 (MQ=60)
                                             ATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTG                                                                                                          <  SRR19973080.4499422/150‑1 (MQ=60)
                                             ATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTG                                                                                                          <  SRR19973080.4498371/150‑1 (MQ=60)
                                             ATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTG                                                                                                          <  SRR19973080.1188564/150‑1 (MQ=60)
                                               TGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAG                                                                                                        >  SRR19973080.3612489/1‑150 (MQ=60)
                                                GGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGG                                                                                                       >  SRR19973080.4079083/1‑150 (MQ=60)
                                                 GCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGA                                                                                                      <  SRR19973080.1998165/150‑1 (MQ=60)
                                                   TGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACG                                                                                                    >  SRR19973080.1206627/1‑150 (MQ=60)
                                                    GCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGA                                                                                                   <  SRR19973080.1356623/150‑1 (MQ=60)
                                                     CAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAA                                                                                                  <  SRR19973080.3561437/150‑1 (MQ=60)
                                                         CTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTC                                                                                              <  SRR19973080.2409597/150‑1 (MQ=60)
                                                         CTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTC                                                                                              <  SRR19973080.2409616/150‑1 (MQ=60)
                                                           GGGCTTTCGCCAGATCACCTTTCGCACTGTAGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTG                                                                                            <  SRR19973080.1774761/150‑1 (MQ=60)
                                                             GCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGATACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCG                                                                                          <  SRR19973080.884915/150‑1 (MQ=60)
                                                             GCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCG                                                                                          <  SRR19973080.884964/150‑1 (MQ=60)
                                                               TTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGAT                                                                                        >  SRR19973080.2020051/1‑150 (MQ=60)
                                                               TTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGAT                                                                                        >  SRR19973080.2019717/1‑150 (MQ=60)
                                                                TTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATC                                                                                       >  SRR19973080.1350914/1‑150 (MQ=60)
                                                                 TCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCC                                                                                      >  SRR19973080.896098/1‑150 (MQ=60)
                                                                  CGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCG                                                                                     >  SRR19973080.380162/1‑150 (MQ=60)
                                                                  CGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCG                                                                                     <  SRR19973080.3059163/150‑1 (MQ=60)
                                                                  CGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCG                                                                                     <  SRR19973080.3060393/150‑1 (MQ=60)
                                                                  CGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCG                                                                                     <  SRR19973080.2378384/150‑1 (MQ=60)
                                                                   GCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTTATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGG                                                                                    >  SRR19973080.3104597/1‑150 (MQ=60)
                                                                    CCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGT                                                                                   >  SRR19973080.3210204/1‑150 (MQ=60)
                                                                     CAGATCACCTTTCGCTCTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTA                                                                                  <  SRR19973080.4160226/150‑1 (MQ=60)
                                                                       GATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGG                                                                                >  SRR19973080.3904866/1‑150 (MQ=60)
                                                                       GATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGG                                                                                >  SRR19973080.3904901/1‑150 (MQ=60)
                                                                         TCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCA                                                                              >  SRR19973080.73614/1‑150 (MQ=60)
                                                                           ACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACT                                                                            <  SRR19973080.3153819/150‑1 (MQ=60)
                                                                           ACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACT                                                                            >  SRR19973080.1434522/1‑150 (MQ=60)
                                                                           ACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACT                                                                            <  SRR19973080.3516486/150‑1 (MQ=60)
                                                                            CCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTG                                                                           >  SRR19973080.1055547/1‑150 (MQ=60)
                                                                                 CGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCG                                                                      >  SRR19973080.3446462/1‑150 (MQ=60)
                                                                                  GCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGA                                                                     >  SRR19973080.3364562/1‑150 (MQ=60)
                                                                                  GCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGA                                                                     >  SRR19973080.3364566/1‑150 (MQ=60)
                                                                                  GCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCGTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGA                                                                     >  SRR19973080.1439432/1‑150 (MQ=60)
                                                                                  GCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTCCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGA                                                                     >  SRR19973080.827551/1‑150 (MQ=60)
                                                                                   CACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAC                                                                    >  SRR19973080.4384230/1‑150 (MQ=60)
                                                                                   CACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAC                                                                    >  SRR19973080.4383314/1‑150 (MQ=60)
                                                                                         CGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAA                                                              <  SRR19973080.3923998/150‑1 (MQ=60)
                                                                                          GTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAG                                                             >  SRR19973080.1339582/1‑150 (MQ=60)
                                                                                           TATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGC                                                            <  SRR19973080.3491666/150‑1 (MQ=60)
                                                                                           TATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGC                                                            <  SRR19973080.3491531/150‑1 (MQ=60)
                                                                                                CGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGT                                                       <  SRR19973080.500764/150‑1 (MQ=60)
                                                                                                CGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGT                                                       <  SRR19973080.500767/150‑1 (MQ=60)
                                                                                                CGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGT                                                       <  SRR19973080.500754/150‑1 (MQ=60)
                                                                                                       TAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATC                                                >  SRR19973080.1540896/1‑150 (MQ=60)
                                                                                                       TAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATC                                                <  SRR19973080.3431917/150‑1 (MQ=60)
                                                                                                                 TATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTT                                      >  SRR19973080.2689821/1‑150 (MQ=60)
                                                                                                                 TATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTT                                      >  SRR19973080.412768/1‑150 (MQ=60)
                                                                                                                 TATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTT                                      >  SRR19973080.2689738/1‑150 (MQ=60)
                                                                                                                  ATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTC                                     >  SRR19973080.4249232/1‑150 (MQ=60)
                                                                                                                  ATCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTC                                     >  SRR19973080.4443906/1‑150 (MQ=60)
                                                                                                                   TCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCA                                    <  SRR19973080.679596/150‑1 (MQ=60)
                                                                                                                   TCAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCA                                    <  SRR19973080.3046213/150‑1 (MQ=60)
                                                                                                                   TCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCA                                    <  SRR19973080.4192406/150‑1 (MQ=60)
                                                                                                                   TCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCA                                    <  SRR19973080.4192424/150‑1 (MQ=60)
                                                                                                                    CAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAG                                   >  SRR19973080.1037291/1‑150 (MQ=60)
                                                                                                                    CAATCTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAG                                   >  SRR19973080.1038957/1‑150 (MQ=60)
                                                                                                                    CAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAG                                   <  SRR19973080.2183591/150‑1 (MQ=60)
                                                                                                                    CAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAG                                   <  SRR19973080.4091184/150‑1 (MQ=60)
                                                                                                                     AATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGT                                  <  SRR19973080.3896192/150‑1 (MQ=60)
                                                                                                                        CTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTT                               <  SRR19973080.1601176/150‑1 (MQ=60)
                                                                                                                        CTGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTT                               <  SRR19973080.1601184/150‑1 (MQ=60)
                                                                                                                         TGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTG                              <  SRR19973080.3211885/150‑1 (MQ=60)
                                                                                                                         TGATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTG                              >  SRR19973080.3602519/1‑150 (MQ=60)
                                                                                                                          GATAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGA                             >  SRR19973080.4361760/1‑150 (MQ=60)
                                                                                                                            TAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACT                           <  SRR19973080.1958593/150‑1 (MQ=60)
                                                                                                                            TAGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACT                           <  SRR19973080.1433275/150‑1 (MQ=60)
                                                                                                                             AGAGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTG                          >  SRR19973080.2831579/1‑150 (MQ=60)
                                                                                                                               AGAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTT                        <  SRR19973080.2152579/150‑1 (MQ=60)
                                                                                                                                GAGAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTA                       <  SRR19973080.1417915/150‑1 (MQ=60)
                                                                                                                                 AGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTAC                      <  SRR19973080.3909238/150‑1 (MQ=60)
                                                                                                                                  GAGACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACT                     >  SRR19973080.4415160/1‑150 (MQ=60)
                                                                                                                                     ACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACG                  <  SRR19973080.16448/150‑1 (MQ=60)
                                                                                                                                     ACACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACG                  <  SRR19973080.16396/150‑1 (MQ=60)
                                                                                                                                      CACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGC                 >  SRR19973080.1035925/1‑150 (MQ=60)
                                                                                                                                       ACCTGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCC                <  SRR19973080.2487791/150‑1 (MQ=60)
                                                                                                                                          TGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAC             <  SRR19973080.1546959/150‑1 (MQ=60)
                                                                                                                                          TGCTTCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAAC             >  SRR19973080.687636/1‑150 (MQ=60)
                                                                                                                                              TCGATGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCG         >  SRR19973080.1355741/1‑150 (MQ=60)
                                                                                                                                               CGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGG        <  SRR19973080.2064571/150‑1 (MQ=60)
                                                                                                                                                  TGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCA     <  SRR19973080.2239173/150‑1 (MQ=60)
                                                                                                                                                  TGTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCA     <  SRR19973080.2239176/150‑1 (MQ=60)
                                                                                                                                                   GTTGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCAT    >  SRR19973080.3480476/1‑150 (MQ=60)
                                                                                                                                                     TGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCT  <  SRR19973080.4384920/150‑1 (MQ=60)
                                                                                                                                                     TGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCT  >  SRR19973080.533315/1‑150 (MQ=60)
                                                                                                                                                     TGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCT  >  SRR19973080.533235/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
AGTACCGAGCAGTTTCTGATAACGATTCACCGTCAATTGCGCGATATTGGCTGCAGCCTGGGCTTTCGCCAGATCACCTTTCGCACTGTCGTATGTCGCCTGATAGGTCGCAGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAGGTTCAGTTTTGACTGTTACTACGCCAACGGCGGGCATCT  >  CP009273/480671‑480969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑