Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,766,617 A→T 17.2% intergenic (+75/‑152) ydiM → / → ydiN MFS superfamily sugar transport 1 family protein/MFS transporter superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,766,6170AT17.2% 592.3 / 108.1 220intergenic (+75/‑152)ydiM/ydiNMFS superfamily sugar transport 1 family protein/MFS transporter superfamily protein
Reads supporting (aligned to +/- strand):  ref base A (88/92);  new base T (22/16);  total (110/110)
Fisher's exact test for biased strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 221 total aligned reads displayed.

TTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGA  >  CP009273/1766471‑1766766
                                                                                                                                                  |                                                                                                                                                     
tttATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCa                                                                                                                                                    <  2:3234201/150‑1 (MQ=255)
 ttATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCaa                                                                                                                                                   >  2:1253375/1‑150 (MQ=255)
  tATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCaaa                                                                                                                                                  <  1:1953622/150‑1 (MQ=255)
  tATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCGAACTAAGGAAAATCGCGATCaaa                                                                                                                                                  <  2:533051/150‑1 (MQ=255)
         ttACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGACAATCGCGATCAAaaacaaac                                                                                                                                           >  1:2069277/1‑150 (MQ=255)
          tACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACt                                                                                                                                          >  2:1458748/1‑150 (MQ=255)
           aCGCAGCCTCAAAAAAACGCGGAATCACTCTCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTa                                                                                                                                         <  2:4158192/150‑1 (MQ=255)
           aCGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTa                                                                                                                                         <  1:114192/150‑1 (MQ=255)
               aGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGTTCAAAAACAAACTATGAc                                                                                                                                     <  1:4480101/150‑1 (MQ=255)
               aGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGAc                                                                                                                                     >  2:2218766/1‑150 (MQ=255)
                 ccGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACAt                                                                                                                                   <  1:1011994/150‑1 (MQ=255)
                 ccGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACAt                                                                                                                                   <  1:3957585/150‑1 (MQ=255)
                      aaaaaaCGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAat                                                                                                                              >  1:4181707/1‑150 (MQ=255)
                       aaaaaCGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAata                                                                                                                             >  1:2464676/1‑150 (MQ=255)
                        aaaaCGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGTTCAAAAACAAACTATGACATGCAatat                                                                                                                            <  2:2914876/150‑1 (MQ=255)
                            cgcgGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGTTCAAAAACAAACTATGACATGCAATATTCCt                                                                                                                        >  1:1007336/1‑150 (MQ=255)
                            cgcgGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGTTCAAAAACAAACTATGACATGCAATATTCCt                                                                                                                        >  1:3285538/1‑150 (MQ=255)
                            cgcgGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCt                                                                                                                        <  1:4153171/150‑1 (MQ=255)
                            cgcgGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCt                                                                                                                        <  1:2436351/150‑1 (MQ=255)
                                 cATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAATGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGaaa                                                                                                                   >  1:3651773/1‑150 (MQ=255)
                                  aTCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGTTCAAAAACAAACTATGACATGCAATATTCCTGGAAAc                                                                                                                  <  1:1535533/150‑1 (MQ=255)
                                  aTCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAAc                                                                                                                  >  2:3024019/1‑150 (MQ=255)
                                    cACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACAt                                                                                                                >  1:3875471/1‑150 (MQ=255)
                                        cGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAAc                                                                                                            <  2:1378431/150‑1 (MQ=255)
                                        cGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAAc                                                                                                            <  2:2655041/150‑1 (MQ=255)
                                        cGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAAc                                                                                                            <  2:2655571/150‑1 (MQ=255)
                                         gCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGTTCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACt                                                                                                           >  2:1678303/1‑150 (MQ=255)
                                          cTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACtt                                                                                                          >  2:4516660/1‑150 (MQ=255)
                                          cTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACtt                                                                                                          <  1:440856/150‑1 (MQ=255)
                                          cTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACtt                                                                                                          >  1:2844991/1‑150 (MQ=255)
                                           tAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACttt                                                                                                         <  1:3449601/150‑1 (MQ=255)
                                           tAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACttt                                                                                                         <  2:3663560/150‑1 (MQ=255)
                                           tAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACttt                                                                                                         <  2:888943/150‑1 (MQ=255)
                                            aaaGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTa                                                                                                        >  2:2079534/1‑150 (MQ=255)
                                               ggAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGTTCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGc                                                                                                     <  2:3180797/150‑1 (MQ=255)
                                               ggAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGc                                                                                                     >  1:3705012/1‑150 (MQ=255)
                                                 aaaaTGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCa                                                                                                   <  1:3095244/150‑1 (MQ=255)
                                                 aaaaTGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCa                                                                                                   <  1:829496/150‑1 (MQ=255)
                                                    aTGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGt                                                                                                >  1:2965219/1‑150 (MQ=255)
                                                      gTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGTTCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTAc                                                                                              <  2:624220/150‑1 (MQ=255)
                                                      gTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTAc                                                                                              <  2:1389336/150‑1 (MQ=255)
                                                           tCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAgg                                                                                         <  1:2694221/150‑1 (MQ=255)
                                                             cGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGa                                                                                       >  1:733285/1‑150 (MQ=255)
                                                              gggtgggtAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGaa                                                                                      >  1:3215935/1‑150 (MQ=255)
                                                              gggtgggtAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGaa                                                                                      <  2:3948370/150‑1 (MQ=255)
                                                               ggtgggtAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGaaa                                                                                     <  1:4510076/150‑1 (MQ=255)
                                                               ggtgggtAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGaaa                                                                                     <  2:3651773/150‑1 (MQ=255)
                                                                gtgggtAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGaaaa                                                                                    <  2:2319520/150‑1 (MQ=255)
                                                                 tgggtAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAat                                                                                   >  2:1296672/1‑150 (MQ=255)
                                                                  gggtAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAatc                                                                                  <  2:4306567/150‑1 (MQ=255)
                                                                  gggtAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAatc                                                                                  <  2:4305078/150‑1 (MQ=255)
                                                                  gggtAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAatc                                                                                  >  1:516626/1‑150 (MQ=255)
                                                                     tAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAatcatc                                                                               >  1:2284110/1‑150 (MQ=255)
                                                                       aTGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCtt                                                                             <  1:3414796/150‑1 (MQ=255)
                                                                       aTGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCtt                                                                             >  1:353322/1‑150 (MQ=255)
                                                                               ttCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAg                                                                     <  1:3348972/150‑1 (MQ=255)
                                                                                 cTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGTTCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTa                                                                   >  1:1881975/1‑150 (MQ=255)
                                                                                  ttttCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTaa                                                                  >  2:2588049/1‑150 (MQ=255)
                                                                                    ttCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAAtt                                                                >  1:2039409/1‑150 (MQ=255)
                                                                                      cAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTAt                                                              >  1:1637291/1‑150 (MQ=255)
                                                                                       aGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATg                                                             >  2:4525421/1‑150 (MQ=255)
                                                                                        ggTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGt                                                            >  1:4284818/1‑150 (MQ=255)
                                                                                           cATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGTTCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTaaa                                                         >  1:2498609/1‑150 (MQ=255)
                                                                                           cATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTaaa                                                         <  2:2339738/150‑1 (MQ=255)
                                                                                            aTGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGTTCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAAc                                                        <  1:1572086/150‑1 (MQ=255)
                                                                                              gCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCg                                                      >  1:4516488/1‑150 (MQ=255)
                                                                                                 aGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGTTCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCg                                                   <  1:2064797/150‑1 (MQ=255)
                                                                                                 aGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCg                                                   >  2:272719/1‑150 (MQ=255)
                                                                                                  gATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCgg                                                  <  2:1199605/150‑1 (MQ=255)
                                                                                                   aTCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGga                                                 >  1:4200087/1‑150 (MQ=255)
                                                                                                        aCGTATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACa                                            <  2:3370292/150‑1 (MQ=255)
                                                                                                         cGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACaa                                           >  1:336263/1‑150 (MQ=255)
                                                                                                           gATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACaaaa                                         >  1:2603940/1‑148 (MQ=255)
                                                                                                           gATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAAta                                         >  2:3777126/1‑150 (MQ=255)
                                                                                                              aaataaCTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAAtacgt                                      >  2:3858777/1‑150 (MQ=255)
                                                                                                               aataaCTCTTTCTGCGCTAACTAAGGAAAATCGCGTTCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAAtacgta                                     >  2:3375621/1‑150 (MQ=255)
                                                                                                               aataaCTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAAtacgta                                     <  1:40260/150‑1 (MQ=255)
                                                                                                                ataaCTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAAtacgtac                                    <  2:1024527/150‑1 (MQ=255)
                                                                                                                ataaCTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAAtacgtac                                    >  2:2605547/1‑150 (MQ=255)
                                                                                                                 taaCTCTTTCTGCGCTAACTAAGGAAAATCGCGCTCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAAtacgtacg                                   <  2:1825751/150‑1 (MQ=255)
                                                                                                                    ctctTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAACCCGTCGGAGAACAATACGGACGGTa                                >  2:1756775/1‑150 (MQ=255)
                                                                                                                       tttCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACg                             >  2:2548379/1‑150 (MQ=255)
                                                                                                                         tCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGaa                           >  1:3952307/1‑150 (MQ=255)
                                                                                                                          cTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGaaa                          <  1:3999653/150‑1 (MQ=255)
                                                                                                                           tGCGCTAACTAAGGAAAATCGCGCTCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAAt                         <  1:1437961/150‑1 (MQ=255)
                                                                                                                           tGCGCTAACTAAGGAAAATCGCGATCCAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAAt                         <  1:3853859/150‑1 (MQ=255)
                                                                                                                             cgcTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTa                       >  1:3910963/1‑150 (MQ=255)
                                                                                                                              gcTAACTCAGGAAAATCGCGATCAAAACCAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTAt                      <  2:1973534/150‑1 (MQ=255)
                                                                                                                                taactaaGGAAAATCGCGTTCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCt                    <  1:1071379/150‑1 (MQ=255)
                                                                                                                                   ctaaGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTc                 <  1:4363309/150‑1 (MQ=255)
                                                                                                                                    taaGGAAAATCGCGTTCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCa                >  1:4354290/1‑150 (MQ=255)
                                                                                                                                     aaGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAg               <  1:3694317/150‑1 (MQ=255)
                                                                                                                                          aaaTCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAgg          >  1:1131913/1‑150 (MQ=255)
                                                                                                                                           aaTCGCGTTCAAAAACAAACTCTGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGa         <  1:1543673/150‑1 (MQ=255)
                                                                                                                                            aTCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCttt                  >  2:954438/1‑140 (MQ=255)
                                                                                                                                            aTCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCttt                  <  1:954551/140‑1 (MQ=255)
                                                                                                                                            aTCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAg        <  1:2121747/150‑1 (MQ=255)
                                                                                                                                                cgATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCtgt    <  1:3835299/150‑1 (MQ=255)
                                                                                                                                                 gATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCtgtg   >  2:2451612/1‑150 (MQ=255)
                                                                                                                                                  aTCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGa  >  2:1867385/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TTTATCGGCTTACGCAGCCGCAAAAAAACGCGGCATCACTCGCTAAAGGAAAATGTCGCTCCGGGTGGGTAATGCAATATTCTTTTCAGGTCATGCAAGATCTTACGGATAAATAACTCTTTCTGCGCTAACTAAGGAAAATCGCGATCAAAAACAAACTATGACATGCAATATTCCTGGAAACATAAACTTTATGCCATGTACCCAGGGAAAATCATCTTCAGTATAGTAATTATGTAAACCGTCGGAGAACAATACGTACGGTAACGAAATTATCTTTCAGCAAGGAGCTGTGA  >  CP009273/1766471‑1766766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A